FlyBase Allele Report: Dmel\InR[CA.UAS.cUa]

General Information

Symbol

Dmel\InR^CA.UAS.cUa

Species

D. melanogaster

Name

FlyBase ID

FBal0321362

Feature type

allele

Associated gene

Dmel\InR

Associated Insertion(s)

Carried in Construct

P{UAS-InR.CA.U}

Also Known As

UAS-InR^CA, UAS-InR^del

Key Links

Allele class

Mutagen

in vitro construct - regulatory fusion

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Acevedo et al., 2015)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{UAS-InR.CA.U}

Cytology

Nature of the lesion

Statement

Reference

UAS regulatory sequences drive expression of a constitutively active form of InR.

(Acevedo et al., 2015)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

InR

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol dependence

CEA

(Acevedo et al., 2015)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal chemosensitive behavior | third instar larval stage, with Scer\GAL4^Or47a.PU

(Slankster et al., 2020)

abnormal chemosensitive behavior | third instar larval stage | calorie restriction conditional, with Scer\GAL4^Orco.PU

(Slankster et al., 2020)

chemical resistant | adult stage, with Scer\GAL4^elav.PU

(Acevedo et al., 2015)

chemical resistant | adult stage, with Scer\GAL4^NP1514

(Acevedo et al., 2015)

Phenotype Manifest In

adult pars intercerebralis, with Scer\GAL4^{Stat92E-0092-G4}

(Brent and Rajan, 2020)

terminal bouton | adult stage | increased number, with Scer\GAL4^{Stat92E-0092-G4}

(Brent and Rajan, 2020)

Detailed Description

Statement

Reference

Expression of InR^CA.UAS.cUa under the control of Scer\GAL4^{Stat92E-0092-G4} (in combination with tub-Gal80[ts] to restrict expression to the adult stage) leads to an increase in the presynaptic bouton number of the STAT-expressing pars intercerebralis neurons in the adult brain.

(Brent and Rajan, 2020)

Unlike controls, expression of InR^CA.UAS.cUa under the control of Scer\GAL4^Orco.PU leads to a significant reduction in attraction to the pentyl acetate in starved larvae when compared to non-starved larvae; and more dramatically under the control of Scer\GAL4^Or47a.PU it leads to the repulsion of both starved and non-starved larvae by pentyl acetate.

Expression of InR^CA.UAS.cUa does not alter the levels of food intake (under the control of Scer\GAL4^Orco.PU or Scer\GAL4^Or47a.PU) although it leads to a significant increase in body weight (under the control of Scer\GAL4^Orco.PU) relative to control larvae.

(Slankster et al., 2020)

Expression of InR^CA.UAS.cUa under the control of Scer\GAL4^Gr28b.b.4.7 in adults does not affect food intake of starved or satiated flies, compared to controls.

(Zhan et al., 2016)

Expression of InR^{CA.Scer\UAS.cUa} under the control of Scer\GAL4^elav.PU (in combination with a Gal80[ts] transgene to restrict expression to the adult stage) results in reduced sensitivity to ethanol-induced sedation, and no change in overall locomotor activity, as compared to controls.

Expression of InR^{CA.Scer\UAS.cUa} under the control of Scer\GAL4^{RhoGAP18B-NP1514} results in reduced sensitivity to ethanol-induced sedation, as compared to controls.

(Acevedo et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Scer\GAL4^NP1514/InR^CA.UAS.cUa is a non-suppressor of chemical sensitive | adult stage phenotype of Arf6^unspecified

(Acevedo et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of InR^{CA.Scer\UAS.cUa} under the control of Scer\GAL4^{RhoGAP18B-NP1514} does not suppress the increased ethanol sensitivity of Arf51F^unspecified/Arf51F^unspecified mutant flies.

(Acevedo et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

InR^{CA.Scer\UAS.cUa}

InR^CA.UAS.cUa

InR^CA

(Jang et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

Open Close