FlyBase Allele Report: Dmel\Cyt-c-p[k13905]

General Information

Symbol

Dmel\Cyt-c-p^k13905

Species

D. melanogaster

Name

FlyBase ID

FBal0321474

Feature type

allele

Associated gene

Dmel\Cyt-c-p

Associated Insertion(s)

P{lacW}Cyt-c-p^k13905

Carried in Construct

Also Known As

l(2)k13905

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Arama et al., 2006)

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}Cyt-c-p^k13905

Cytology

Description

The P{lacW}Cyt-c-p^k13905 insertion is lethal over Df(2L)Exel6039 (a deficiency that removes both Cyt-c-p and Cyt-c-d) and this lethality can be rescued by transgenic expression of either Cyt-c-p or Cyt-c-d. However, the lethality of P{lacW}Cyt-c-p^k13905 homozygotes cannot be rescued, suggesting that in addition to lethality associated with the insertion, the chromosome carries an additional unrelated lethal mutation.

(Arama et al., 2006)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:16,719,978..16,719,978 [+]2L:16,719,978..16,719,978 [+]

(Kahsai et al., 2016, Arama et al., 2006, Arama et al., 2003, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Lysates from 4 hour old prepupae heterozygous for Cyt-c-p^k13905 show no reduction in caspase activation compared to wild type.

(Dorstyn et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Cyt-c-p^k13905/Df(2L)Exel6039 has lethal phenotype, suppressible by Scer\GAL4^Tub.PU/Cyt-c-d^UAS.cAa

(Arama et al., 2006)

Cyt-c-p^k13905 has lethal phenotype, suppressible by Cyt-c-d^Z21091

(Arama et al., 2006)

Suppressor of

Statement

Reference

Cyt-c-p^k13905 is a suppressor of male sterile phenotype of Cyt-c-d^Z21091

(Arama et al., 2006)

Cyt-c-p^k13905/l(2)k13905[+] is a suppressor of visible | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

Phenotype Manifest In

Suppressor of

Statement

Reference

Cyt-c-p^k13905/l(2)k13905[+] is a suppressor of eye | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Cyt-c-d^Z21091 complements the lethality of Cyt-c-p^k13905, and Cyt-c-p^k13905 complements the sterility of Cyt-c-d^Z21091. Expression of the Cyt-c-d^Scer\UAS.cAa transgene, under the control of Scer\GAL4^tub, rescues the lethality of Cyt-c-p^k13905/Df(2L)Exel6039 flies.

(Arama et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Cyt-c-p^k13905 is rescued by Scer\GAL4^Tub.PU/Cyt-c-p^UAS.cAb

(Arama et al., 2006)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

11115

y¹ w^67c23; P{lacW}Cyt-c-p^k13905/CyO

Kyoto

111334

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}Cyt-c-p^k13905 P{neoFRT}40A/CyO y⁺

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Deficiency complementation analysis is consistent with the lethality of the line being associated with the insertion.

(Kahsai et al., 2016)

Comments

The Cyt-c-p^k13905 chromosome appears to carry a separable lethal mutation, which cannot be rescued by expression of a wild-type Cyt-c-p transgene.

(Arama et al., 2006)

Complements: Cas^k03902. Complements: glu^k08819. Complements: Mhc^k10423. Complements: l(2)k15102^k15102.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Cyt-c-p^k13905

l(2)k13905

(Arama et al., 2006, Dorstyn et al., 2004, Spradling et al., 1999)

l(2)k13905^k13905

Name Synonyms

Secondary FlyBase IDs

FBal0160645
FBal0064115

References (7)

Report Sections

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