FlyBase Allele Report: Dmel\Atg14[Δ5.2]

General Information

Symbol

Dmel\Atg14^Δ5.2

Species

D. melanogaster

Name

FlyBase ID

FBal0326158

Feature type

allele

Associated gene

Dmel\Atg14

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Katheder et al., 2017)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

P{EPgy2}Atg14^EY14568

(Katheder et al., 2017)

Cytology

Nature of the lesion

Statement

Reference

1191bp deletion that removes the translation start site and two-thirds of the coding region of Atg14.

(Katheder et al., 2017)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by scrib⁶⁷³, Ras85D^V12.UAS

(Katheder et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage | somatic clone

(Katheder et al., 2017)

abnormal size | somatic clone | third instar larval stage

(Katheder et al., 2017)

lethal

(Katheder et al., 2017)

visible | adult stage | somatic clone

(Katheder et al., 2017)

Phenotype Manifest In

eye | somatic clone

(Katheder et al., 2017)

eye-antennal disc | somatic clone | third instar larval stage

(Katheder et al., 2017)

Detailed Description

Statement

Reference

Atg14^Δ5.2 homozygotes are lethal before adulthood.

Atg14^Δ5.2 homozygous clones in mosaic third instar larval eye-antennal imaginal discs occupy a significantly smaller proportion of the disc area and result in decreased eye sizes in adults, as compared to control clones.

(Katheder et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Atg14^Δ5.2/Atg14^Δ5.2 is a suppressor | cell non-autonomous | partially of abnormal size | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg14^EY14568/Atg14^Δ5.2 is a suppressor of abnormal size | somatic clone | third instar larval stage phenotype of Atg13^Δ81, Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg14^EY14568/Atg14^Δ5.2 is a suppressor of neoplasia | somatic clone | third instar larval stage phenotype of Atg13^Δ81, Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg14^Δ5.2/Atg14^Δ5.2 is a suppressor | cell non-autonomous | partially of neoplasia | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg13^Δ81, Atg13^Δ81, Atg14^Δ5.2, Atg14^Δ5.2 is a suppressor | partially | somatic clone of abnormal size | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg13^Δ81, Atg13^Δ81, Atg14^Δ5.2, Atg14^Δ5.2 is a suppressor | partially | somatic clone of neoplasia | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

NOT Suppressor of

Statement

Reference

Atg14^{+tCH322-175F03}, Atg14^EY14568, Atg14^Δ5.2 is a non-suppressor of abnormal size | somatic clone | third instar larval stage phenotype of Atg13^Δ81, Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg14^{+tCH322-175F03}, Atg14^EY14568, Atg14^Δ5.2 is a non-suppressor of neoplasia | somatic clone | third instar larval stage phenotype of Atg13^Δ81, Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Phenotype Manifest In

Suppressor of

Statement

Reference

Atg14^Δ5.2/Atg14^Δ5.2 is a suppressor | cell non-autonomous | partially of eye-antennal disc | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Atg13^Δ81, Atg13^Δ81, Atg14^Δ5.2, Atg14^Δ5.2 is a suppressor | partially | somatic clone of eye-antennal disc | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, scrib⁶⁷³

(Katheder et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The size of scrib⁶⁷³-homozygous, Ras85D^V12.Scer\UAS-expressing eye-antennal imaginal disc clone tumors is significantly suppressed either by Atg14^Δ5.2 homozygosity in neighboring disc cells or by the combination of Atg13^Δ81 homozygosity in clone cells plus Atg14^Δ5.2 homozygosity in neighboring disc cells; these scrib⁶⁷³-homozygous, Ras85D^V12.Scer\UAS-expressing tumors grow to significantly smaller sizes when grafted into the abdomen of Atg14^Δ5.2/Atg14^EY14568 transheterozygous adult hosts (but not if the hosts also possess one copy of Atg14^{+tCH322-175F03}), as compared to control hosts.

(Katheder et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Atg14^Δ5.2 is rescued by Atg14^{+tCH322-175F03}

(Katheder et al., 2017)

Comments

The lethality of Atg14^Δ5.2 homozygotes is rescued by the presence of one copy of Atg14^{+tCH322-175F03}.

(Katheder et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Atg14^Δ5.2

(Billes et al., 2018)

atg14^Δ5.2

(Katheder et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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