FB2021_02, released April 13, 2021

Allele: Hsap\RELAAD.R30B01

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General Information
Symbol
Hsap\RELAAD.R30B01
Species
H. sapiens
Name
FlyBase ID
FBal0328687
Feature type
allele
Associated gene
Hsap\RELA
Associated Insertion(s)
Carried in Construct
P{R30B01-p65.AD}
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Rubin Laboratory at Janelia Farm, 2017)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{R30B01-p65.AD}
      Cytology
      Nature of the lesion
      Statement
      Reference

      The GMR30B01 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

      (Rubin Laboratory at Janelia Farm, 2017)
      Allele components
      Product class / Tool use(s)
      Encoded product / tool
      p65(AD)::Zip+
      Associated Sequence Features
      GMR30B01
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult subesophageal zone | restricted

      Comment: when combined with P{R31H10-GAL4.DBD}

      (Hampel et al., 2017)
      dorsal pedicellar sensillum campaniformium | subset

      Comment: when combined with P{R31H10-GAL4.DBD}

      (Hampel et al., 2017)
      dorsal scabellar sensillum campaniformium | subset

      Comment: when combined with P{R31H10-GAL4.DBD}

      (Hampel et al., 2017)
      mechanosensory neuron of wing margin bristle | subset

      Comment: when combined with P{R31H10-GAL4.DBD}

      (Hampel et al., 2017)
      sensory neuron of sensillum campaniformium of distal group | subset

      Comment: when combined with P{R31H10-GAL4.DBD}

      (Hampel et al., 2017)
      sensory neuron of sensillum campaniformium of ventral radius | subset

      Comment: when combined with P{R31H10-GAL4.DBD}

      (Hampel et al., 2017)
      adult ventral nerve cord | restricted

      Comment: when combined with P{R31H10-GAL4.DBD}

      (Hampel et al., 2017)
      ventral pedicellar sensillum campaniformium | subset

      Comment: when combined with P{R31H10-GAL4.DBD}

      (Hampel et al., 2017)
      ventral scabellar sensillum campaniformium | subset

      Comment: when combined with P{R31H10-GAL4.DBD}

      (Hampel et al., 2017)
      Additional Information
      Statement
      Reference

      In a split-GAL4 system, Hsap\RELAAD.R30B01 drives expression in 5-10 (out of 77) sensillum campaniformium neurons in the proximal part of the wing, a majority of distal 5-6 (out of 8) campaniformium neurons in the distal part of the wing, 7-10 (out of approximately 139) sensillum campaniformium neurons in the halteres and 3-5 (out of approximately 221) mechanosensory neurons that are part of the wing margin bristle when combined with Scer\GAL4DBD.R31H10. Neurons on the wings and halteres send projections to the thoracico-abdominal ganglion, where some ascend to the adult subesophageal zone.

      (Hampel et al., 2017)
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with red light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R31H10 and Hsap\RELAAD.R30B01 elicits significant wing (but not haltere) grooming behavior, compared to controls; this grooming behavior persists after light cessation.

      (Hampel et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\RELAAD.R30B01.T:Zzzz\Zip+
      Hsap\RELAAD.R30B01
      Name Synonyms
      Secondary FlyBase IDs
        References (3)