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General Information
Symbol
Scer\GAL4DBD.R71D01
Species
S. cerevisiae
Name
FlyBase ID
FBal0332309
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
R71D01-DBD, R71D01-ZpGdbd
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

The GMR71D01 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

Allele components
Product class / Tool use(s)
Associated Sequence Features
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

In a split-GAL4 system, Scer\GAL4DBD.R71D01 drives expression in antennal grooming descending neuron 1 when combined with Hsap\RELAAD.R18C11, in antennal grooming brain interneuron 1 and the adult central complex when combined with Hsap\RELAAD.R11B11, and in antennal grooming descending neuron 1, antennal grooming brain interneuron 1, the optic lobe and thoracico-abdominal ganglion when combined with Hsap\RELAAD.R34C03. Antennal grooming descending neuron 1 neurons were seen in close proximity to Ecol\lexAGMR26B12 expressing antennal grooming brain interneuron 2 and Ecol\lexAGMR27H08 expressing zone C/E Johnston organ neurons. Positive GRASP (GFP reconstitution across synaptic partners) signals were seen between C/E Johnston organ neurons and antennal grooming descending neuron 1 using these drivers.

 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Thermogenetic activation (using TrpA1Scer\UAS.(B).cKa at 30-31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R71D01 and Hsap\RELAAD.R11B11 or Scer\GAL4DBD.R71D01 and Hsap\RELAAD.R18C11 results in a significant increase of antennal grooming behavior compared to control flies; this phenotype persists following antennal amputation.

Thermogenetic activation (using TrpA1Scer\UAS.(B).cKa at 30-31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R71D01 and Hsap\RELAAD.R34C03 results in a significant increase of antennal grooming behavior compared to control flies; there is a further significant increase in antennal grooming behavior following antennal amputation.

Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with red light activation) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R71D01 and Hsap\RELAAD.R18C11 induces antennal grooming, which does not persist beyond (some grooming bouts even ending prior) to red light cessation.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Inhibition of synaptic release (using Ctet\TeTxLCTNT.Scer\UAS) in neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R71D01 and Hsap\RELAAD.R11B11 significantly partially suppresses the increase in antennal grooming seen in flies with thermogenetic activation (using TrpA113xlexAop.JFRC26 at 30-31[o]C) of Ecol\lexAGMR27H08 neurons.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Scer\GAL4DBD.R71D01.T:Zzzz\Zip-
Scer\GAL4DBD.R71D01
Name Synonyms
Secondary FlyBase IDs
    References (10)