FlyBase Allele Report: Scer\GAL4[DBD.R71D01]

General Information

Symbol

Scer\GAL4^DBD.R71D01

Species

S. cerevisiae

Name

FlyBase ID

FBal0332309

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{R71D01-GAL4.DBD}

Also Known As

R71D01-DBD, R71D01-ZpGdbd

Allele class

Mutagen

in vitro construct - regulatory fusion

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Rubin Laboratory at Janelia Farm, 2017)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{R71D01-GAL4.DBD}

Cytology

Nature of the lesion

Statement

Reference

The GMR71D01 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

(Rubin Laboratory at Janelia Farm, 2017)

Allele components

Product class / Tool use(s)

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Associated Sequence Features

GMR71D01

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

medullary intrinsic neuron Mi1

Comment: when combined with P{R19F01-p65.AD}

when combined with P{R19F01-p65.AD} (combination referred to as 'SS00809')

when combined with P{R55C05-p65.AD}

(Strother et al., 2018, Strother et al., 2017, Takemura et al., 2017)

adult central complex

Comment: when combined with P{R11B11-p65.AD}

(Hampel et al., 2015)

antennal grooming brain interneuron 1

Comment: when combined with P{R11B11-p65.AD}

when combined with P{R34C03-p65.AD}

(Hampel et al., 2015)

antennal grooming descending neuron 1

Comment: when combined with P{R18C11-p65.AD}

when combined with P{R34C03-p65.AD}

(Hampel et al., 2015)

adult optic lobe | restricted

Comment: when combined with P{R34C03-p65.AD}

(Hampel et al., 2015)

adult ventral nerve cord | restricted

Comment: when combined with P{R34C03-p65.AD}

(Hampel et al., 2015)

Additional Information

Statement

Reference

In a split-GAL4 system, Scer\GAL4^DBD.R71D01 drives expression in antennal grooming descending neuron 1 when combined with Hsap\RELA^AD.R18C11, in antennal grooming brain interneuron 1 and the adult central complex when combined with Hsap\RELA^AD.R11B11, and in antennal grooming descending neuron 1, antennal grooming brain interneuron 1, the optic lobe and thoracico-abdominal ganglion when combined with Hsap\RELA^AD.R34C03. Antennal grooming descending neuron 1 neurons were seen in close proximity to Ecol\lexA^GMR26B12 expressing antennal grooming brain interneuron 2 and Ecol\lexA^GMR27H08 expressing zone C/E Johnston organ neurons. Positive GRASP (GFP reconstitution across synaptic partners) signals were seen between C/E Johnston organ neurons and antennal grooming descending neuron 1 using these drivers.

(Hampel et al., 2015)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Thermogenetic activation (using TrpA1^{Scer\UAS.(B).cKa} at 30-31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R71D01 and Hsap\RELA^AD.R11B11 or Scer\GAL4^DBD.R71D01 and Hsap\RELA^AD.R18C11 results in a significant increase of antennal grooming behavior compared to control flies; this phenotype persists following antennal amputation.

Thermogenetic activation (using TrpA1^{Scer\UAS.(B).cKa} at 30-31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R71D01 and Hsap\RELA^AD.R34C03 results in a significant increase of antennal grooming behavior compared to control flies; there is a further significant increase in antennal grooming behavior following antennal amputation.

Optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with red light activation) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R71D01 and Hsap\RELA^AD.R18C11 induces antennal grooming, which does not persist beyond (some grooming bouts even ending prior) to red light cessation.

(Hampel et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Hsap\RELA^AD.R11B11, Ctet\tetX^TNT-LC.UAS, Scer\GAL4^DBD.R71D01 is a suppressor | partially of abnormal grooming behavior | heat sensitive phenotype of Ecol\lexA^GMR27H08, TrpA1^{13xlexAop.JFRC26}

(Hampel et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Inhibition of synaptic release (using Ctet\TeTxLC^TNT.Scer\UAS) in neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R71D01 and Hsap\RELA^AD.R11B11 significantly partially suppresses the increase in antennal grooming seen in flies with thermogenetic activation (using TrpA1^{13xlexAop.JFRC26} at 30-31[o]C) of Ecol\lexA^GMR27H08 neurons.

(Hampel et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

69066

w¹¹¹⁸; P{R71D01-GAL4.DBD}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^{DBD.R71D01.T:Zzzz\Zip-}

Scer\GAL4^DBD.R71D01

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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