FB2025_01 , released February 20, 2025
Allele: Dmel\PolD3L27
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General Information
Symbol
Dmel\PolD3L27
Species
D. melanogaster
Name
FlyBase ID
FBal0350362
Feature type
allele
Associated gene
Dmel\PolD3
Associated Insertion(s)
P{?EPgy2}PolD3L27
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Ji et al., 2019)
Mutagen
P-element activity
(Ji et al., 2019)
Progenitor genotype
P{EPgy2}PolD3EY15283
(Ji et al., 2019)
Associated Insertion(s)
P{?EPgy2}PolD3L27
Cytology
Description

Mobilization of the progenitor P{EPgy2}Pol32EY15283 insertion, resulting in deletion of nt15255461 to nt15256304 with an additional 162bp of filler sequences from the P element. Since only part of the Pol32 coding region is removed, along with the entire annotated 3'UTR, there is still a possibility that a truncated Pol32 protein with 205 residues is produced, though this is undetectable by Western Blot analyses.

(Ji et al., 2019)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
complex_substitution
2L:15,255,461..15,256,304 [-]
Comment:

An 844 bp deletion resulting from the imprecise excision of P{EPgy2}Pol32EY15283. Reported to map to 2L:15255461..15256304 . 162 bases of P element sequences remain at the site of the excision.

(Ji et al., 2019)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      A small proportion of Pol32L30/Pol32L27 third instar larvae show double strand breaks, which are nearly absent in controls.

      (Ji et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Other
      Statement
      Reference

      PolD1I10/DNApol-delta[+], PolD3L30/PolD3L27 has visible phenotype

      (Ji et al., 2019)

      Df(3L)BSC122/+, PolD3L30/PolD3L27 has visible phenotype

      (Ji et al., 2019)

      PolD2G16501/Pol31[+], PolD3L30/PolD3L27 has visible phenotype

      (Ji et al., 2019)

      DNApol-alpha180[+]/PolA1G13925, PolD3L30/PolD3L27 has visible phenotype

      (Ji et al., 2019)

      Df(3R)Exel6186/+, PolD3L30/PolD3L27 has visible phenotype

      (Ji et al., 2019)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      A small proportion of Pol32L30/Pol32L27 third instar larvae show double strand breaks, both centric and non-centric, which are nearly absent in controls; these become much more frequent in a DNApol-δI10 heterozygous background and are both centric and non-centric.

      A very small proportion of Pol32L30/Pol32L27 adults show an etched abdomen phenotype; this phenotype is only slightly increased in a DNApol-α180G13925/+ background, is moderately increased in a Pol31G16501/+ background, becomes highly prevalent in Df(3L)BSC122/+ or Df(3R)Exel6186/+ backgrounds and becomes nearly fully penetrant in a DNApol-δI10/+ background; the phenotype is not much affected in Df(3R)X3F/+, spn-A1/+, Df(3R)P-21/+, or BlmD2/+ backgrounds.

      (Ji et al., 2019)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Pol32L27
      PolD3L27
      pol32L27
      (Ji et al., 2019)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)