FlyBase Allele Report: Dmel\kis[10D26]

General Information

Symbol

Dmel\kis^10D26

Species

D. melanogaster

Name

FlyBase ID

FBal0351577

Feature type

allele

Associated gene

Dmel\kis

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Gervais et al., 2019)

Progenitor genotype

Cytology

Description

Point mutation (C2317T) in the transcript that encodes the long kis isoform; this is predicted to result in a premature stop codon (FlyBase curator comment: the mutation is reported as occurring at residue Q772, but there is no Q residue at position 772 in the reference sequence, instead the change that introduces the premature stop codon has been mapped to residue Q771).

(Gervais et al., 2019)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:239,877..239,877 [-]

Nucleotide change:

C239877T

Reported nucleotide change:

C2317T

Amino acid change:

Reported amino acid change:

Q772term

Comment:

The mutation is reported to be at aa residue Q772 (codon CAA) but there is no Gln at position 772 in the reference sequence. It was mapped to Q771.

(Gervais et al., 2019)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | adult stage | recessive | somatic clone

(Gervais et al., 2019)

increased occurrence of cell division | adult stage | recessive | somatic clone

(Gervais et al., 2019)

lethal | recessive

(Gervais et al., 2019)

Phenotype Manifest In

adult enterocyte | somatic clone

(Gervais et al., 2019)

adult enteroendocrine cell | somatic clone

(Gervais et al., 2019)

adult midgut epithelium | somatic clone

(Gervais et al., 2019)

intestinal stem cell | adult stage | somatic clone

(Gervais et al., 2019)

Detailed Description

Statement

Reference

As a result of stem cell overproliferation, kis^10D26/kis^10D26 somatic clones in a heterozygous background contain a greater overall number of cells and significantly more intestinal stem cells, enteroendocrine cells and enterocytes in adult flies compared to controls. The ratio between enteroendocrine cells and enterocytes is unaffected.

(Gervais et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

kis^10D26 has increased cell number | adult stage | recessive | somatic clone phenotype, non-enhanceable by Scer\GAL4^Tub.PU/trr^JF03242

(Gervais et al., 2019)

Suppressed by

Statement

Reference

kis^10D26 has increased cell number | recessive | somatic clone | adult stage phenotype, suppressible by Scer\GAL4^Tub.PU/N^{cdc10.UAS.Tag:MYC}

(Gervais et al., 2019)

kis^10D26 has increased cell number | recessive | somatic clone | adult stage phenotype, suppressible by Scer\GAL4^Tub.PU/Egfr^DN.UAS.cBa

(Gervais et al., 2019)

kis^10D26 has increased cell number | recessive | somatic clone | adult stage phenotype, suppressible by Scer\GAL4^Tub.PU/cic^UAS.Tag:HA

(Gervais et al., 2019)

kis^10D26 has increased cell number | recessive | somatic clone | adult stage phenotype, suppressible by bsk^DN.UAS/Scer\GAL4^Tub.PU

(Gervais et al., 2019)

kis^10D26 has increased cell number | recessive | somatic clone | adult stage phenotype, suppressible by dome^HMS01293/Scer\GAL4^Tub.PU

(Gervais et al., 2019)

kis^10D26 has increased cell number | recessive | somatic clone | adult stage phenotype, suppressible by InR^K1409A.UAS/Scer\GAL4^Tub.PU

(Gervais et al., 2019)

kis^10D26 has increased cell number | recessive | somatic clone | adult stage phenotype, suppressible by yki^HMS00041/Scer\GAL4^Tub.PU

(Gervais et al., 2019)

NOT suppressed by

Statement

Reference

kis^10D26 has increased cell number | recessive | somatic clone | adult stage phenotype, non-suppressible by Scer\GAL4^Tub.PU/N^{cdc10.UAS.Tag:MYC}

(Gervais et al., 2019)

kis^10D26 has increased cell number | recessive | somatic clone | adult stage phenotype, non-suppressible by Scer\GAL4^Tub.PU/trr^JF03242

(Gervais et al., 2019)

Enhancer of

Statement

Reference

kis^10D26/kis^10D26 is an enhancer of increased cell number | adult stage | somatic clone phenotype of Scer\GAL4^Tub.PU, trr^JF03242

(Gervais et al., 2019)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

kis^10D26 has adult midgut epithelium | somatic clone phenotype, non-enhanceable by Scer\GAL4^Tub.PU/trr^JF03242

(Gervais et al., 2019)

kis^10D26 has intestinal stem cell | adult stage | somatic clone phenotype, non-enhanceable by Scer\GAL4^Tub.PU/trr^JF03242

(Gervais et al., 2019)

Suppressed by

Statement

Reference

kis^10D26 has adult midgut epithelium | somatic clone phenotype, suppressible by InR^K1409A.UAS/Scer\GAL4^Tub.PU

(Gervais et al., 2019)

kis^10D26 has adult midgut epithelium | somatic clone phenotype, suppressible by yki^HMS00041/Scer\GAL4^Tub.PU

(Gervais et al., 2019)

kis^10D26 has intestinal stem cell | somatic clone | adult stage phenotype, suppressible by Scer\GAL4^Tub.PU/Egfr^DN.UAS.cBa

(Gervais et al., 2019)

kis^10D26 has intestinal stem cell | somatic clone | adult stage phenotype, suppressible by Scer\GAL4^Tub.PU/cic^UAS.Tag:HA

(Gervais et al., 2019)

kis^10D26 has intestinal stem cell | somatic clone | adult stage phenotype, suppressible by bsk^DN.UAS/Scer\GAL4^Tub.PU

(Gervais et al., 2019)

kis^10D26 has intestinal stem cell | somatic clone | adult stage phenotype, suppressible by dome^HMS01293/Scer\GAL4^Tub.PU

(Gervais et al., 2019)

kis^10D26 has intestinal stem cell | somatic clone | adult stage phenotype, suppressible by InR^K1409A.UAS/Scer\GAL4^Tub.PU

(Gervais et al., 2019)

kis^10D26 has intestinal stem cell | somatic clone | adult stage phenotype, suppressible by yki^HMS00041/Scer\GAL4^Tub.PU

(Gervais et al., 2019)

kis^10D26 has intestinal stem cell | somatic clone phenotype, suppressible by Scer\GAL4^Tub.PU/N^{cdc10.UAS.Tag:MYC}

(Gervais et al., 2019)

kis^10D26 has adult midgut epithelium | somatic clone phenotype, suppressible by Scer\GAL4^Tub.PU/Egfr^DN.UAS.cBa

(Gervais et al., 2019)

kis^10D26 has adult midgut epithelium | somatic clone phenotype, suppressible by Scer\GAL4^Tub.PU/cic^UAS.Tag:HA

(Gervais et al., 2019)

kis^10D26 has adult midgut epithelium | somatic clone phenotype, suppressible by bsk^DN.UAS/Scer\GAL4^Tub.PU

(Gervais et al., 2019)

kis^10D26 has adult midgut epithelium | somatic clone phenotype, suppressible by dome^HMS01293/Scer\GAL4^Tub.PU

(Gervais et al., 2019)

NOT suppressed by

Statement

Reference

kis^10D26 has adult enterocyte | somatic clone phenotype, non-suppressible by Scer\GAL4^Tub.PU/N^{cdc10.UAS.Tag:MYC}

(Gervais et al., 2019)

kis^10D26 has adult midgut epithelium | somatic clone phenotype, non-suppressible by Scer\GAL4^Tub.PU/trr^JF03242

(Gervais et al., 2019)

kis^10D26 has intestinal stem cell | somatic clone | adult stage phenotype, non-suppressible by Scer\GAL4^Tub.PU/trr^JF03242

(Gervais et al., 2019)

Enhancer of

Statement

Reference

kis^10D26/kis^10D26 is an enhancer of adult midgut epithelium | adult stage | somatic clone phenotype of Scer\GAL4^Tub.PU, trr^JF03242

(Gervais et al., 2019)

kis^10D26/kis^10D26 is an enhancer of intestinal stem cell | adult stage | somatic clone phenotype of Scer\GAL4^Tub.PU, trr^JF03242

(Gervais et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

kis^EC1

(Gervais et al., 2019)

kis^LM27

(Gervais et al., 2019)

kis¹

(Gervais et al., 2019)

Rescued by

kis^10D26 is rescued by kis^{UAS.L.Tag:polyHis,Tag:FLAG}/Scer\GAL4^Tub.PU

(Gervais et al., 2019)

Partially rescued by

kis^10D26 is partially rescued by kis^FLAP

(Gervais et al., 2019)

kis^10D26 is partially rescued by kis^{K2060R.UAS.L.Tag:polyHis,Tag:FLAG}/Scer\GAL4^Tub.PU

(Gervais et al., 2019)

Not rescued by

kis^10D26 is not rescued by Scer\GAL4^Tub.PU/kis^{UAS.S.Tag:FLAG}

(Gervais et al., 2019)

Comments

Expressing kis^{UAS.L.Tag:polyHis,Tag:FLAG} (but not kis^{UAS.S.Tag:FLAG}) under the control of Scer\GAL4^Tub.PU fully rescues the increased overall number of cells and of intestinal stem cells in kis^10D26/kis^10D26 somatic clones, while kis^FLAP or expressing kis^{K2060R.UAS.L.Tag:polyHis,Tag:FLAG} results in only a partial rescue.

(Gervais et al., 2019)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

kis^10D26

(Gervais et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (1)

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