actin, F-actin, BAP47, 5C actin, β-actin
Gene model reviewed during 5.55
Gene model reviewed during 5.46
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.56
The BRM complex was purified from Drosophila embryos using two distinct purification protocols and the subunit composition was determined. Eight major proteins were consistently observed including brm, Snr1, Bap55 (a novel actin-related protein), Bap60, Bap111, Hsc70-4, and mor proteins. The complex also contains a cytoplasmic actin (Act42A or Act5C).
Interacts with Rab6.
Oxidation of Met-45 by Mical to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Act5C using the Feature Mapper tool.
Comment: reported as dorsal/lateral sensory complexes
GBrowse - Visual display of RNA-Seq signalsView Dmel\Act5C in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Candidate stable intronic sequence RNA (sisRNA) identified within 5'UTR of this gene.
DNA-protein interactions: genome-wide binding profile assayed for Act5C protein in Kc167 cells; see Chromatin_types_NKI collection report. Individual protein-binding experiments listed under "Samples" at GEO_GSE22069 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22069).
S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.
dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a binucleation phenotype with microtubule extensions when assayed in Kc167 cells.
Age related changes in Act5C expression have been studied by Northern analysis.
Analysis of Act5C promoter activation shows that one cis-acting region in P\Tcore and two such regions in P\TKP and P\TKP' elements are associated with activation only when the 20bp sequence around the Zw transcription start site is placed in front of the Act5C promoter. In vitro transcription analysis of P\T sequences activating Zw-Act5C transcriptional gene reveals three distinct cis-acting regions, one in P\Tcore and two in the P\TKP and P\TKP' elements, that are required for overexpression. Putative transcriptional regulatory proteins, identified in gel retardation assays, bind to each of the cis-acting regions.
Nascent chain nuclear run-on assays in KC161 cells reveal different responses to heat shock for different genes. Transcription of His1 is severely inhibited under mild heat shocks, of Act5C decreases proportionally with increasing temperature while that of the core histone genes or the heat shock cognates is repressed only under extreme heat shock. Increased transcription of the heat shock genes is observed within 1-2 mins of heat shock and maximal rates were reached within 2-5 minutes. Rates of transcription vary over a 20-fold range.
Sequence analysis demonstrates insect muscle actins form a distinct family of closely related proteins significantly diverged from cytoplasmic actins.
Translation of actin RNA in early embryos injected with initiation factors has been studied.
Detailed analysis of the proximal promoter and the elements required for its function.
The expression of HIS-C genes during oogenesis has been studied, and compared to periods of DNA synthesis and actin expression during this developmental stage.
Codes for cytoplasmic actin; transcribed throughout development; one of two actin genes transcribed in Kc cells and several other cell lines (FBrf0038990). One of three actin genes expressed in the wing disc during wing development, each with its characteristic profile. Peak expression at 44h of pupal age, little or no expression at 60h rising again at 80h. 44h peak corresponds to time of maximum change in cell shape (FBrf0042391). Transcripts present in the preblastoderm embryo suggesting maternal transcription; during blastoderm formation, Act5C transcripts accumulate at the periphery of the embryo; local concentrations of transcript observed in anterior and posterior midgut rudiments in stage 13 embryos; hybridization also observed in the developing ventral nervous system. Later in embryogenesis, transcript found in all tissues but with dramatic concentrations of transcripts in the anterior and posterior midgut and the proventriculus. Exon specific probes demonstrate that transcripts containing exon 1 tend to be concentrated in anterior portions of early embryos, including the anterior midgut primordium and the proventriculus, as well as in the posterior midgut primordium; during germ-band extension, small local concentrations of exon 2 transcripts are seen in posterior and ventral regions of the embryo (FBrf0049492). A ecdysone regulated gene (FBrf0064343). The Act5C distal promoter is often used in constructs to give 'constitutive' expression of a sequence under its control.