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General Information
Symbol
Dmel\Act5C
Species
D. melanogaster
Name
Actin 5C
Annotation Symbol
CG4027
Feature Type
FlyBase ID
FBgn0000042
Gene Model Status
Stock Availability
Gene Summary
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Multiple isoforms are involved in various cellular functions such as cytoskeleton structure, cell mobility, chromosome movement and muscle contraction. (UniProt, P10987)
Contribute a Gene Snapshot for this gene.
Also Known As

actin, F-actin, BAP47, 5C actin, β-actin

Key Links
Genomic Location
Cytogenetic map
Sequence location
X:5,900,861..5,905,399 [+]
Recombination map
1-14
RefSeq locus
NC_004354 REGION:5900861..5905399
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (7 terms)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (5 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from expression pattern
involved_in tube formation
inferred from genetic interaction with FLYBASE:eys; FB:FBgn0031414
inferred from genetic interaction with FLYBASE:prom; FB:FBgn0259210
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred by curator from GO:0031011
inferred from biological aspect of ancestor with PANTHER:PTN002631617
(assigned by GO_Central )
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (0 terms)
Protein Family (UniProt)
Belongs to the actin family. (P10987)
Summaries
Gene Group (FlyBase)
ACTINS -
Actins are highly conserved eukaryotic filament-forming proteins that are major components of the cytoskeleton. Actin polymerisation is controlled by ATP binding and hydrolysis. Actins are involved in various cell processes including cell movement, muscle contraction, cell trafficking and mechanical support. (Adapted from PMID:21859859).
BRAHMA ASSOCIATED PROTEINS COMPLEX -
The Brahma associated proteins (BAP) complex is an ATP-dependent chromatin remodeling complex. (Adapted from FBrf0192510.)
INO80 COMPLEX -
The INO80 complex is an ATP-dependent chromatin remodeller that can mobilize nucleosomes. (Adapted from PMID:17316710).
POLYBROMO-CONTAINING BRAHMA ASSOCIATED PROTEINS COMPLEX -
Brahma (BRM) complexes are ATP-dependent chromatin remodeling complexes. The Polybromo-containing Brahma associated proteins (PBAP) complex is distinguished by the presence of polybromo and Bap170 subunits. (Adapted from FBrf0192510.)
NON-CANONICAL BRAHMA ASSOCIATED PROTEINS COMPLEX -
Brahma (BRM) complexes are ATP-dependent chromatin remodeling complexes. The non-canonical BAP (ncBAP) complex is distinguished by the presence of a Bicra subunit. (Adapted from FBrf0247407.)
Protein Function (UniProtKB)
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Multiple isoforms are involved in various cellular functions such as cytoskeleton structure, cell mobility, chromosome movement and muscle contraction.
(UniProt, P10987)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
Act5C: Actin in region 5C
Codes for cytoplasmic actin; transcribed throughout development; one of two actin genes transcribed in Kc cells and several other cell lines (Fyrberg, Mahaffy, Bond, and Davidson, 1983, Cell 33: 115-23). One of three actin genes expressed in the wing disc during wing development, each with its characteristic profile. Peak expression at 44h of pupal age, little or no expression at 60h rising again at 80h. 44h peak corresponds to time of maximum change in cell shape (Peterson, Bond, Mitchell, and Davidson, 1985, Dev. Genet. 5: 219-25). Transcripts present in the preblastoderm embryo suggesting maternal transcription; during blastoderm formation, Act5C transcripts accumulate at the periphery of the embryo; local concentrations of transcript observed in anterior and posterior midgut rudiments in stage 13 embryos; hybridization also observed in the developing ventral nervous system. Later in embryogenesis, transcript found in all tissues but with dramatic concentrations of transcripts in the anterior and posterior midgut and the proventriculus. Exon specific probes demonstrate that transcripts containing exon 1 tend to be concentrated in anterior portions of early embryos, including the anterior midgut primordium and the proventriculus, as well as in the posterior midgut primordium; during germ-band extension, small local concentrations of exon 2 transcripts are seen in posterior and ventral regions of the embryo (Burn, Vigoreaux, and Tobin, 1989, Dev. Biol. 131: 345-55).
Gene Model and Products
Number of Transcripts
5
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Act5C for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.55

Gene model reviewed during 5.46

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0070822
1637
376
FBtr0070823
2653
376
FBtr0100662
2252
376
FBtr0100663
1928
376
FBtr0345894
2863
376
Additional Transcript Data and Comments
Reported size (kB)

1.97 (northern blot)

1.9, 1.77 (northern blot)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0070787
41.8
376
5.16
FBpp0070788
41.8
376
5.16
FBpp0100124
41.8
376
5.16
FBpp0100125
41.8
376
5.16
FBpp0311818
41.8
376
5.16
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments

Small Act5C-derived peptides identified by nano LC-MS/MS: SSSSLEKSYELPDGQVI.

The brm core complex appears to comprise 10 polypeptides. These include brm, mor, osa, Bap60, Snr1, Bap111, Bap55, Bap170, Bap26,and Act42A or Act5C proteins.

The BRM complex was purified from Drosophila embryos using two distinct purification protocols and the subunit composition was determined. Eight major proteins were consistently observed including brm, Snr1, Bap55 (a novel actin-related protein), Bap60, Bap111, Hsc70-4, and mor proteins. The complex also contains a cytoplasmic actin (Act42A or Act5C).

External Data
Subunit Structure (UniProtKB)

Interacts with Rab6.

(UniProt, P10987)
Post Translational Modification

Oxidation of Met-45 by Mical to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.

(UniProt, P10987)
Crossreferences
PDB - An information portal to biological macromolecular structures
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Act5C using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
expression microarray
Stage
Tissue/Position (including subcellular localization)
Reference
adult fat body

Comment: cyclical, peak ZT9-12

in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism | ubiquitous

Comment: maternally deposited

Additional Descriptive Data

Expressed cyclically in the adult fat body.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
Expression Deduced from Reporters
Stage
Tissue/Position (including subcellular localization)
Reference
Stage
Tissue/Position (including subcellular localization)
Reference
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{Act5C-GAL4.Hb}
Stage
Tissue/Position (including subcellular localization)
Reference
Stage
Tissue/Position (including subcellular localization)
Reference
Stage
Tissue/Position (including subcellular localization)
Reference
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{act-lacZ.B}
Stage
Tissue/Position (including subcellular localization)
Reference
Stage
Tissue/Position (including subcellular localization)
Reference
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Act5C in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 9-10
  • Stages(s) 13-16
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 46 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 22 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Act5C
Transgenic constructs containing regulatory region of Act5C
Aberrations (Deficiencies and Duplications) ( 3 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
border follicle cell & filopodium
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (43)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
11 of 15
Yes
Yes
4  
10 of 15
No
Yes
1  
5 of 15
No
No
5 of 15
No
No
5 of 15
No
Yes
4 of 15
No
No
4 of 15
No
No
4 of 15
No
Yes
1  
4 of 15
No
No
4 of 15
No
No
3 of 15
No
Yes
3 of 15
No
No
3 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
1  
3 of 15
No
No
1  
3 of 15
No
Yes
3 of 15
No
Yes
2 of 15
No
No
2  
2 of 15
No
No
1  
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1  
2 of 15
No
No
1  
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1  
1 of 15
No
No
6  
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (28)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
12 of 15
Yes
Yes
11 of 15
No
No
4 of 15
No
Yes
4 of 15
No
No
4 of 15
No
No
4 of 15
No
Yes
4 of 15
No
No
4 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
Yes
3 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
Rattus norvegicus (Norway rat) (30)
10 of 13
Yes
Yes
7 of 13
No
Yes
7 of 13
No
Yes
4 of 13
No
Yes
4 of 13
No
Yes
4 of 13
No
No
4 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
No
3 of 13
No
Yes
3 of 13
No
Yes
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (14)
11 of 12
Yes
Yes
10 of 12
No
Yes
3 of 12
No
Yes
3 of 12
No
Yes
3 of 12
No
No
3 of 12
No
Yes
3 of 12
No
No
2 of 12
No
No
2 of 12
No
No
2 of 12
No
No
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
Danio rerio (Zebrafish) (22)
11 of 15
Yes
Yes
11 of 15
Yes
Yes
4 of 15
No
Yes
4 of 15
No
Yes
4 of 15
No
No
4 of 15
No
Yes
4 of 15
No
No
4 of 15
No
Yes
3 of 15
No
Yes
3 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (12)
13 of 15
Yes
Yes
12 of 15
No
Yes
11 of 15
No
Yes
6 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (16)
9 of 9
Yes
Yes
9 of 9
Yes
Yes
8 of 9
No
Yes
8 of 9
No
Yes
8 of 9
No
Yes
8 of 9
No
No
8 of 9
No
Yes
4 of 9
No
Yes
3 of 9
No
Yes
3 of 9
No
Yes
2 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
Yes
1 of 9
No
No
Saccharomyces cerevisiae (Brewer's yeast) (8)
12 of 15
Yes
Yes
0  
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
Schizosaccharomyces pombe (Fission yeast) (2)
10 of 12
Yes
Yes
1 of 12
No
No
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091909C7 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila simulans
Drosophila sechellia
Drosophila sechellia
Drosophila erecta
Drosophila erecta
Drosophila yakuba
Drosophila yakuba
Drosophila ananassae
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila pseudoobscura pseudoobscura
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila persimilis
Drosophila willistoni
Drosophila willistoni
Drosophila virilis
Drosophila virilis
Drosophila mojavensis
Drosophila mojavensis
Drosophila grimshawi
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915055R )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Glossina morsitans
Tsetse fly
Glossina morsitans
Tsetse fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Lucilia cuprina
Australian sheep blowfly
Lucilia cuprina
Australian sheep blowfly
Lucilia cuprina
Australian sheep blowfly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Mayetiola destructor
Hessian fly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W05GN )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X05CQ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G08LD )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (14)
8 of 10
7 of 10
7 of 10
7 of 10
7 of 10
5 of 10
5 of 10
4 of 10
4 of 10
4 of 10
3 of 10
3 of 10
3 of 10
2 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 8 )
    Modifiers Based on Experimental Evidence ( 3 )
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    RNA-protein
    Physical Interaction
    Assay
    References
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Interacts with Rab6.
    (UniProt, P10987 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    X
    Recombination map
    1-14
    Cytogenetic map
    Sequence location
    X:5,900,861..5,905,399 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    5C7-5C7
    Limits computationally determined from genome sequence between P{EP}CG15770EP390 and P{EP}Act5CEP1604&P{EP}EP444
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    5C3-5C6
    (determined by in situ hybridisation) 5C5--8 (determined by in situ hybridisation) 5D1--2 (determined by in situ hybridisation) 5C3--8 (determined by in situ hybridisation) 5C5--6 (determined by in situ hybridisation) 5C5--10 (determined by in situ hybridisation)
    5C5-5C10
    (determined by in situ hybridisation) 5C5--8 (determined by in situ hybridisation)
    5C3-5C8
    (determined by in situ hybridisation) 5C5--8 (determined by in situ hybridisation)
    5C5-5C8
    (determined by in situ hybridisation) 5C5--6 (determined by in situ hybridisation) 5C3--9 (determined by in situ hybridisation) 5D1--2 (determined by in situ hybridisation)
    5C3-5C6
    (determined by in situ hybridisation) 5C5--8 (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (75)
    Genomic Clones (7)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (240)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for database merge of

    Source for merge of: Act5C anon- EST:fe2D2

    Source for merge of: Act5C l(1)G0009

    Source for merge of: Act5C l(1)G0079 l(1)G0330 l(1)G0025 l(1)G0117 l(1)G0420 l(1)G0010 l(1)G0177 l(1)G0245 l(1)G0486

    Additional comments

    The Act5C gene may be derived from the Act57B gene by retroposition.

    "l(1)G0449" may affect "Act5C".

    Other Comments

    Candidate stable intronic sequence RNA (sisRNA) identified within 5'UTR of this gene.

    DNA-protein interactions: genome-wide binding profile assayed for Act5C protein in Kc167 cells; see Chromatin_types_NKI collection report. Individual protein-binding experiments listed under "Samples" at GEO_GSE22069 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22069).

    S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

    dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.

    RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a binucleation phenotype with microtubule extensions when assayed in Kc167 cells.

    Act5C has a role in the individualisation during spermatogenesis.

    Age related changes in Act5C expression have been studied by Northern analysis.

    Ecdysteroid-regulated gene.

    The level of Act5C RNA remains fairly constant throughout the D.melanogaster life span.

    Analysis of Act5C promoter activation shows that one cis-acting region in P\Tcore and two such regions in P\TKP and P\TKP' elements are associated with activation only when the 20bp sequence around the Zw transcription start site is placed in front of the Act5C promoter. In vitro transcription analysis of P\T sequences activating Zw-Act5C transcriptional gene reveals three distinct cis-acting regions, one in P\Tcore and two in the P\TKP and P\TKP' elements, that are required for overexpression. Putative transcriptional regulatory proteins, identified in gel retardation assays, bind to each of the cis-acting regions.

    Nascent chain nuclear run-on assays in KC161 cells reveal different responses to heat shock for different genes. Transcription of His1 is severely inhibited under mild heat shocks, of Act5C decreases proportionally with increasing temperature while that of the core histone genes or the heat shock cognates is repressed only under extreme heat shock. Increased transcription of the heat shock genes is observed within 1-2 mins of heat shock and maximal rates were reached within 2-5 minutes. Rates of transcription vary over a 20-fold range.

    Act5C promoter driven expression is just as efficient in D.melanogaster embryos and in L.cuprina embryos.

    Sequence analysis demonstrates insect muscle actins form a distinct family of closely related proteins significantly diverged from cytoplasmic actins.

    Translation of actin RNA in early embryos injected with initiation factors has been studied.

    Nuclear extracts prepared from developmentally staged embryos were transcriptionally active for several non-muscle genes: Tm2 non-muscle promoter, Act5C, ftz, Adh and en.

    Detailed analysis of the proximal promoter and the elements required for its function.

    The expression of HIS-C genes during oogenesis has been studied, and compared to periods of DNA synthesis and actin expression during this developmental stage.

    Codes for cytoplasmic actin; transcribed throughout development; one of two actin genes transcribed in Kc cells and several other cell lines (FBrf0038990). One of three actin genes expressed in the wing disc during wing development, each with its characteristic profile. Peak expression at 44h of pupal age, little or no expression at 60h rising again at 80h. 44h peak corresponds to time of maximum change in cell shape (FBrf0042391). Transcripts present in the preblastoderm embryo suggesting maternal transcription; during blastoderm formation, Act5C transcripts accumulate at the periphery of the embryo; local concentrations of transcript observed in anterior and posterior midgut rudiments in stage 13 embryos; hybridization also observed in the developing ventral nervous system. Later in embryogenesis, transcript found in all tissues but with dramatic concentrations of transcripts in the anterior and posterior midgut and the proventriculus. Exon specific probes demonstrate that transcripts containing exon 1 tend to be concentrated in anterior portions of early embryos, including the anterior midgut primordium and the proventriculus, as well as in the posterior midgut primordium; during germ-band extension, small local concentrations of exon 2 transcripts are seen in posterior and ventral regions of the embryo (FBrf0049492). A ecdysone regulated gene (FBrf0064343). The Act5C distal promoter is often used in constructs to give 'constitutive' expression of a sequence under its control.

    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 94 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    PDB - An information portal to biological macromolecular structures
    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DPiM - Drosophila Protein interaction map
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (60)
    Reported As
    Symbol Synonym
    A4V404_DROME
    Act5C
    (Shieh et al., 2021, Yang et al., 2021, Barish et al., 2020, Chetverina et al., 2020, Meyer-Nava et al., 2020, Shilova et al., 2020, Vásquez-Procopio et al., 2020, Guan et al., 2019, Kobb et al., 2019, Luhur et al., 2019, Myat et al., 2019, Ikawa and Sugimura, 2018, Ordonez et al., 2018, Richier et al., 2018, Sokolova et al., 2018, Bower et al., 2017, Min et al., 2017, Orr et al., 2017, Lefebvre et al., 2016, Ogneva et al., 2016, Dong et al., 2015, Kollmar, 2015.9.15, Pek et al., 2015, Ashwal-Fluss et al., 2014, Chapin et al., 2014, DeSalvo et al., 2014, dos Santos, 2014, Hsiao et al., 2014, Merlo et al., 2014, Molnár et al., 2014, Nie et al., 2014, Radermacher et al., 2014, Chan et al., 2013, Dantoft et al., 2013, Kwon et al., 2013, Taliaferro et al., 2013, Wilson et al., 2013, Duan et al., 2012, Kaushik et al., 2012, Matzat et al., 2012, White-Cooper, 2012, Adamson et al., 2011, Chan et al., 2011, Cherbas et al., 2011, Clark et al., 2011, Friedman et al., 2011, Jin et al., 2011, Ling and Salvaterra, 2011, Matta et al., 2011, Rees et al., 2011, Rohn et al., 2011, Toku et al., 2011, Xu et al., 2011, Blanco et al., 2010, Kallappagoudar et al., 2010, Kong et al., 2010, Mathur et al., 2010, Monier et al., 2010, Pauli et al., 2010, Popodi, 2010.10.8, Repiso et al., 2010, Saulière et al., 2010, Sens et al., 2010, Venken et al., 2010, Wasbrough et al., 2010, Wasbrough et al., 2010, Hessle et al., 2009, Tanaka et al., 2009, Bai et al., 2008, Fisher et al., 2008, Hanai et al., 2008, Penney et al., 2008, Yang et al., 2008, Clark et al., 2007, Derré et al., 2007, Fulga et al., 2007, Grieder et al., 2007, Muse et al., 2007, Ninov et al., 2007, Quinones-Coello, 2007, Stuart et al., 2007, Beller et al., 2006, Dorus et al., 2006, Dorus et al., 2006, Jiang and Crews, 2006, Mikhaylova et al., 2006, Montana and Littleton, 2006, Ni et al., 2006, Peyre et al., 2006, Stroschein-Stevenson et al., 2006, Walser et al., 2006, Delanoue and Davis, 2005, Laviolette et al., 2005)
    anon-EST:fe2D2
    chrX:5748184..5748304
    β-actin/Bap47
    Name Synonyms
    Dm-actin5C
    anon-fast-evolving-2D2
    cellular cytoskeletal β-actin
    Secondary FlyBase IDs
    • FBgn0025223
    • FBgn0026673
    • FBgn0026683
    • FBgn0026712
    • FBgn0027215
    • FBgn0027255
    • FBgn0027284
    • FBgn0027327
    • FBgn0028278
    • FBgn0040144
    • FBgn0040204
    Datasets (1)
    Study focus (1)
    Experimental Role
    Project
    Project Type
    Title
    • transgene_used
    Protein profiling reveals five principal chromatin types in Drosophila cells.
    References (749)