Bruno, aret, Bru, arrest, Dm Bru
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.45
4.0, 3.7, 3.3, 2.7 (northern blot)
808, 604 (aa)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\bru1 using the Feature Mapper tool.
bru1 mRNA is highly expressed in developing IFMs at 30 and 72 hours APF and is maintained at lower levels in adult IFMs but is not in tubular leg or jump muscles.
aret expression at embryonic stages 1-4 is localized to the posterior pole of the embryo.
GBrowse - Visual display of RNA-Seq signalsView Dmel\bru1 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Changed from 'aret/arrest' to 'bru1/bruno 1' to reflect the nomenclature used in first paper to characterize the gene (FBrf0082225), the preferred usage in the literature since, and the subsequent discovery of additional members of the bruno family (FBrf0129832).
Source for merge of aret NEST:bs01g04 was sequence comparison ( date:030903 ).
Source for merge of aret BcDNA:GM15173 was a shared cDNA ( date:030728 ).
Cell-free assays indicate that aret represses osk translation by two different mechanisms. The first is by interacting with the cup protein that binds the eIF-4E protein, thus preventing recruitment of the small ribosomal subunit. The second is by inducing oligomerization of osk mRNA which is then sequestered in large RNP complexes that cannot be accessed by the 43S preinitiation complex.
Control of germ cell proliferation.
aret has a function in the cytoblast to cyst transition during early oogenesis.
aret protein regulates multiple mRNAs involved in gametogenesis and early embryogenesis.
One readily detectable protein with an apparent molecular weight of 80kD binds specifically to the nos mRNA 3' untranslated region (3'UTR), the protein is called aret. Binding assays demonstrate aret response elements (BRE) exist in the A, B and C region of osk 3' UTR. aret is required for preventing translation of osk mRNA prior to its localisation at the posterior pole of the oocyte.
Postmeiotic differentiation defect.
Mutations cause the production of few, defective, germ cells. In weak alleles their may be small egg chambers that fail to develop.