Gene model reviewed during 5.49
There is only one protein coding transcript and one polypeptide associated with this gene
1861 (aa); 220 (kD observed)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\asp using the Feature Mapper tool.
asp transcript is expressed in cardiogenic mesoderm at embryonic stage 11, but not in the embryonic/larval heart at stage 16.
asp expression in early embryos is diffusely concentrated at the anterior end of the embryo.
GBrowse - Visual display of RNA-Seq signalsView Dmel\asp in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in unfocused spindle microtubules and pole detachment when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in kinetochore-fiber unfocusing and centrosome detachment phenotypes when assayed in S2 cells.
Mitotic S2 cells treated with dsRNA made from templates generated with primers directed against this gene display a striking detachment of centrosomes from spindles and a loss of spindle pole focusing, resulting in an increase in pole-pole spacing. There is also an increase in spindle length and an elevation of the mitotic index.
One of 42 Drosophila genes identified as being most likely to reveal molecular and cellular mechanisms of nervous system development or plasticity relevant to human Mental Retardation disorders.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Area matching Drosophila EST AA539252. Probable intron in gene represented by EST AA539252.
asp protein appears to hold together the microtubule-nucleating γ tubulin ring complexes that organise the mitotic centrosome.
Five genes essential for cell cycle progression, polo, mgr, asp, stg and gnu, are recombined onto a single chromosome, EMS mutations are isolated that fail to complement this chromosome. Genes encoding proteins that interact with the products of any of the five genes are isolated.
Monopolar spindles in asp mutants are hemispindles in which an asymmetric array of microtubules extend from a single centrosome towards condensed chromosomes.
The hypothesis that most mitotic mutants do not lead to lethality in early development due to the effect of maternally provided wild type products has been tested by studying cuticular clones.
Immunocytological techniques have been used to observe the spindle apparatus in asp mutants and the results indicate that non-disjunction in asp can result from the association of condensed chromosomes with hemi-spindle structures in meiosis and mitosis.
Mutants are larval lethal, with a high frequency of aberrant (e.g. polyploid) cells arrested in metaphase in the larval brain. Postmeiotic stages of spermatogenesis are abnormal, with, e.g. multinucleate spermatids. In the absence of maternal product show severe nuclear cycle defects with free centrosomes.
Required for the structure of both mitotic and meiotic spindles.