Gene model reviewed during 5.44
Stop-codon suppression (UAG) postulated; FBrf0216884.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.46
1638 (aa); 185 (kD)
Component of the Brahma complex, which is composed of brm, osa, mor, Snr1/Bap45, dalao/Bap111, Bap55, Bap60 and Act42A/Bap47 (PubMed:10601025, PubMed:10809665). Interacts with asf1 (PubMed:12381660). Associates with the brm-HDAC3-erm repressor complex, composed of brm, HDAC3 and erm (PubMed:24618901). Interacts with erm and HDAC3 (PubMed:24618901).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\brm using the Feature Mapper tool.
brm transcripts are expressed maximally in unfertilized eggs and early embryos, indicating maternal contributions. Levels drop steadily during embryogenesis with a dramatic drop at 16hrs. Low levels of brm transcripts are observed in larvae, pupae, and adult females. The temporal pattern of brm transcription is very similar to that of Snr1.
GBrowse - Visual display of RNA-Seq signalsView Dmel\brm in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: brm CG18438
Source for merge of brm CG18438 was sequence comparison ( date:001104 ).
A chimeric Scer\snf2-brm protein (in which the DNA-dependent ATPase domain of Scer\snf2 has been replaced with the corresponding region of brm) partially rescues the growth defects of S.cerevisiae Scer\snf2 mutant cells.
Fifteen alleles induced by EMS, three alleles induced by γ irradiation and four alleles induced by hybrid dysgenesis.
DNA-protein interactions: genome-wide binding profile assayed for brm protein in S2 cells; GEO accession number GSE32404.
The 'l(3)72Aa' (brm) complementation group comprises 19 EMS-induced mutant alleles.
DNA-protein interactions: genome-wide binding profile assayed for brm protein in Kc167 cells; see Chromatin_types_NKI collection report. Individual protein-binding experiments listed under "Samples" at GEO_GSE22069 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22069).
dsRNA has been made from templates generated with primers directed against this gene. RNAi of brm disrupts the dendritic routing patterns of the ddaD and ddaE neurons, resulting in aberrantly oriented primary dendrites. RNAi also causes defects in muscle, alterations in the number of MD neurons, defects in dendrite morphogenesis but no obvious defects in da dendrite development.
S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.
D.melanogaster contains two distinct brahma (BRM) complexes: the BAP complex, defined by the presence of osa protein and the absence of both polybromo and Bap170 proteins, and the PBAP complex, which contains the polybromo and Bap170 proteins but lacks the osa protein.
The chromatin remodelling activity of the brm complex may play a general role in facilitating transcription by RNA polymerase II.
Pc, Scm, Psc, ph-p and ph-d contribute to the PRC1 (Polycomb repressive complex 1). PRC1 directly antagonizes ATP-dependent remodeling of nucleosomal arrays in a purified system and may directly modulate (and be modified by) SWI/SNF (brm/mor) activity.
Used in an investigation to address the relationship between retrotransposons and retroviruses and the coadaptation of these retroelements to their host genomes. Results indicate retrotransposons are heterogeneous in contrast to retroviruses, suggesting different modes of evolution by slippage-like mechanisms.
The Snr1 and brm proteins are present in a large complex and co-precipitate from extracts, these results suggest that the Drosophila counterpart of the yeast SWI/SNF complex plays an important role in counteracting the repressive effects of chromatin on homeotic gene transcription during development.
Phenotypic studies and genetic interactions suggest that Snr1 and brm act together, and with trx, to regulate homeotic gene transcription. Snr1 and brm proteins are present in a large complex, this complex may play an important role in maintaining homeotic gene transcription during development by counteracting the repressive effect of chromatin.
Severe abnormalities caused by loss of brm expression demonstrates that homeotic genes are not the only target for brm activation. The complex pattern of interallelic complementation suggests that brm may act as a multimer.
brm is one of the 18 loci identified in a screen for dominant modifiers of Pc and/or Antp phenotypes. Alleles of Pc, Pcl, Scm, Dll, brm, kto, Scr and trx show clear dominant enhancement or suppression of AntpScx, whereas alleles of vtd, Vha55, Su(Pc)37D, urd, mor, skd and osa do not.
"Brahma" means "fate" in India.