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General Information
Symbol
Dmel\chic
Species
D. melanogaster
Name
chickadee
Annotation Symbol
CG9553
Feature Type
FlyBase ID
FBgn0000308
Gene Model Status
Stock Availability
Gene Snapshot
chickadee (chic) encodes an actin monomer binding protein that provides the major cellular pool of readily polymerizing ATP-actin monomers. It is involved in oogenesis, spermatogenesis, cell division, bristle formation, cellular morphogenesis, axon growth, filopodia formation, dorsal closure, wound healing and stem cell maintenance. [Date last reviewed: 2019-03-07]
Also Known As

profilin, sand, stranded, chi

Key Links
Genomic Location
Cytogenetic map
Sequence location
2L:5,972,115..5,981,018 [-]
Recombination map

2-18

RefSeq locus
NT_033779 REGION:5972115..5981018
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (28 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from physical interaction with UniProtKB:Q8T4F7
(assigned by UniProt )
inferred from physical interaction with FLYBASE:dia; FB:FBgn0011202
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000176159
(assigned by GO_Central )
Biological Process (22 terms)
Terms Based on Experimental Evidence (17 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:cib; FB:FBgn0026084
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:Imp; FB:FBgn0285926
inferred from mutant phenotype
inferred from high throughput mutant phenotype
inferred from mutant phenotype
(assigned by CACAO )
inferred from mutant phenotype
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000176159
(assigned by GO_Central )
Cellular Component (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000176159
(assigned by GO_Central )
Protein Family (UniProt)
Belongs to the profilin family. (P25843)
Summaries
Protein Function (UniProtKB)
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. This profilin is required for intercellular cytoplasm transport during Drosophila oogenesis.
(UniProt, P25843)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
chic: chickadee (T. Schupbach)
Female sterile; nurse cell contents are not transported into the egg, and homozygous females lay tiny eggs, which remain unfertilized.
Summary (Interactive Fly)
Gene Model and Products
Number of Transcripts
6
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\chic for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.40

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.44

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0079233
1005
126
FBtr0079235
1402
126
FBtr0079234
1513
126
FBtr0079236
2091
126
FBtr0309212
988
126
FBtr0309213
1275
126
Additional Transcript Data and Comments
Reported size (kB)

1.2, 1.0 (northern blot)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0078864
13.7
126
5.05
FBpp0078866
13.7
126
5.05
FBpp0078865
13.7
126
5.05
FBpp0089196
13.7
126
5.05
FBpp0301151
13.7
126
5.05
FBpp0301152
13.7
126
5.05
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio.

(UniProt, P25843)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\chic using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

The 1.2kb chi transcript is abundant in ovaries and elsewhere in adult males and females. It is present in RNA isolated from the seven chi insertion alleles tested.

The 1.0kb chi transcript is ovary specific. It is absent in all seven chickadee insertion alleles.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\chic in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 57 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 17 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of chic
Transgenic constructs containing regulatory region of chic
Deletions and Duplications ( 13 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
actin filament & eye disc | somatic clone
aster & meiotic cell cycle | male
cleavage furrow & spermatocyte
contractile ring & meiotic cell cycle | male
follicle cell & actin filament | somatic clone
photoreceptor cell & eye disc | somatic clone
spindle & meiotic cell cycle | male
trichogen cell & actin filament & pupa
ventral adult lateral neuron & commissure, with Scer\GAL4P2.4.Pdf
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
9 of 15
Yes
Yes
2 of 15
No
Yes
 
10  
2 of 15
No
Yes
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
10 of 15
Yes
Yes
2 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (7)
7 of 13
Yes
Yes
2 of 13
No
Yes
2 of 13
No
Yes
2 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (3)
3 of 12
Yes
Yes
3 of 12
Yes
Yes
2 of 12
No
Yes
Danio rerio (Zebrafish) (4)
3 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (3)
14 of 15
Yes
Yes
6 of 15
No
Yes
6 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (5)
9 of 9
Yes
Yes
9 of 9
Yes
Yes
8 of 9
No
Yes
8 of 9
No
Yes
8 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
14 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
11 of 12
Yes
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190IFD )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150EUO )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0IFM )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0JB2 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G104O )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Ciona intestinalis
Vase tunicate
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 2 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 2 )
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Dmel gene
Ortholog showing functional complementation
Supporting References
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser

Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
RNA-protein
Physical Interaction
Assay
References
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Subunit Structure (UniProtKB)
Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio.
(UniProt, P25843 )
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
External Data
Linkouts
KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2L
Recombination map

2-18

Cytogenetic map
Sequence location
2L:5,972,115..5,981,018 [-]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
26A5-26B2
Limits computationally determined from genome sequence between P{EP}bchsEP2299&P{EP}GpdhEP466 and P{lacW}chick13321
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
26A5-26A6
(determined by in situ hybridisation)
26A-26A
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (23)
Genomic Clones (14)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (265)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
    Commercially Available Antibodies
     
    Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for database merge of
    Additional comments
    Other Comments

    Identified in an RNAi screen for host factors that alter infection of SL2 cells by M.fortuitum.

    dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.

    dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.

    chic regulates actin filament formation throughout the cell cortex in the follicular epithelium.

    cib and chic cooperate in central brain metamorphosis.

    ena protein can bind directly to chic protein. The proline-rich region of ena is responsible for this interaction.

    Mutations in chic disrupt cytokinesis during male meiosis, preventing the formation of both the central spindle and the contractile ring.

    Identification: Enhancer trap screen designed to discover genes involved in the cellular aspects of defense mechanisms, as well as in melanotic tumor formation processes linked to blood cell dysregulation.

    The chic mutant phenotype resembles that caused by disrupting the actin cytoskeleton with cytochalasin D, and that of capu and spir. Mutants in chic resemble those in capu in that they fail to localize stau protein and osk mRNA to the posterior pole of the developing oocyte.

    The chic profilin product can bind to the product of capu in a yeast two hybrid assay.

    Phenotypic analysis of chic male-sterile alleles reveals defects in meiotic cytokinesis and suggests that proper actin assembly is necessary for centrosome separation and migration.

    Induced expression of chic proteins causes aberrant cell shapes reflecting defects in cytokinesis and/or cel shape maintenance.

    Proliferation defect locus.

    Identification: Screen for mutations affecting neuromuscular connectivity, using an antibody to Fas2.

    The network of cytoplasmic actin filaments in each nurse cell is missing in chic egg chambers: this does not account for the chic phenotype. Cytoplasm transport is disrupted as nuclei are not properly held in position during chic nurse cell regression and when flow starts the nuclei are pushed into the ring canals thus blocking the pathway to the oocyte.

    There are at least 10 P element insertions at chic with effects on both male and female fertility and bristle formation. Female sterile mutation caused by loss of ovary specific transcript. P insert in first exon.

    Promotes actin assembly.

    Mutations at the chic locus cause defects in midoogenesis.

    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 73 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    InterPro - A database of protein families, domains and functional sites
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (18)
    Reported As
    Symbol Synonym
    chic
    (Chougule et al., 2020, Kiss et al., 2020, Meltzer and Schuldiner, 2020.6.25, Oliveira da Silva et al., 2020, Samuels et al., 2020, Genovese et al., 2019, Herrera and Bach, 2019, Meltzer et al., 2019, Thuveson et al., 2019, Ghiglione et al., 2018, Lebreton et al., 2018, Segal et al., 2018, Tsai et al., 2018, Zhang et al., 2018, Cheng et al., 2017, Pinheiro et al., 2017, Qu et al., 2017, Taylor et al., 2017, Wu et al., 2017, Kooij et al., 2016, Matsukawa et al., 2016, Schwartz et al., 2016, Yu et al., 2016, Hosono et al., 2015, Ashwal-Fluss et al., 2014, Medioni et al., 2014, Pézeron et al., 2014, Rudrapatna et al., 2014, Shields et al., 2014, Das et al., 2013, de Celis et al., 2013.9.11, Külshammer and Uhlirova, 2013, Kwon et al., 2013, Zheng et al., 2013, Brock et al., 2012, Giansanti and Fuller, 2012, Giansanti et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Murray et al., 2012, Urwyler et al., 2012, Gonçalves-Pimentel et al., 2011, Rohn et al., 2011, Seong et al., 2011, Xu et al., 2011, Zheng et al., 2011, Barkó et al., 2010, Cunha et al., 2010, Okumura et al., 2010, Sánchez-Soriano et al., 2010, Wang et al., 2010, Delon and Brown, 2009, Dworkin et al., 2009, Jiang et al., 2009, Maybeck and Röper, 2009, Warner and Longmore, 2009, Burnett et al., 2008, Estes et al., 2008, Franciscovich et al., 2008, Johnson et al., 2008, Matusek et al., 2008, Noguchi et al., 2008, Pawson et al., 2008, Wang and Riechmann, 2008, Christensen et al., 2007.10.29, Corrigall et al., 2007, Dahlgaard et al., 2007, Dyer et al., 2007, Neuman-Silberberg, 2007, Ninov et al., 2007, Parrish et al., 2007, Quinones-Coello, 2007, Stuart et al., 2007, Wang and Riechmann, 2007, Christensen and Cook, 2006.8.29, Frank et al., 2006, Molnar et al., 2006, Montana and Littleton, 2006, Oshima, 2006, Jagadeeshan and Singh, 2005, Minakhina et al., 2005, Kim et al., 2001)
    Name Synonyms
    Secondary FlyBase IDs
    • FBgn0013731
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (355)