DHR3, Hr46, Hormone receptor-like in 46, Complementation group D, NR1F4
transcription factor - nuclear receptor superfamily - a second tier regulator involved in Drosophila molting - acts negatively on Ecdysone receptor and postively on genes expressed subsequently in the molting hierarchy
Gene model reviewed during 5.56
Gene model reviewed during 6.13
Gene model reviewed during 5.49
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.44
Stop-codon suppression (UAA) postulated; FBrf0216884
Unconventional translation start (ACG) postulated;
9, 7, 5.5 (northern blot)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Hr3 using the Feature Mapper tool.
Hr3 expression is enriched in border follicle cells relative to other cells in the egg chamber.
Hr36 transcript expression peaks coincide with ecdysteriod peaks in embryos, larvae, and pupae.
Hr46 expression was studied in staged third instar larvae and prepupae collected at two hour intervals. Hr46 transcripts can be detected at low levels in late third instar larvae (116-120hr after egg laying). Expression peaks in 2-4hr prepupae and declines significantly by 8hr after puparium formation. In staged prepupal salivary glands, Hr46 transcripts are abundant in 0-2hr prepupal glands, decrease in 4hr glands, and are undetectable at later stages. Hr46 transcripts are rapidly induced by ecdysone.
Peaks of Hr46 expression are observed at midembryogenesis, during larval instars L1 and L2, at the end of L3 extending into the prepupal stage, and during pupal development. Timing suggests that transcription is ecdysone induced. The 9kb Hr46 transcript is observed only during pupal development.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Hr3 in GBrowse 2
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Hr3 Hr46
One or more of the processed transcripts for these genes contain several non-overlapping open reading frames (ORFs). The non-overlapping ORFs are represented by CG33183 (FBgn0000448), CG12912 (FBgn0033497) and CG46321 (FBgn0284237). Different transcripts of Hr3 (CG33138) are dicistronic for CG12912 and CG46321; no one transcript is polycistronic.
Renamed from 'Hr46' to 'Hr3' to reflect overwhelming usage in the literature and the symbol used in the initial paper to identify the gene (FBrf0057540).
Annotations CG11823 and CG12208 merged as CG33183 in release 3 of the genome annotation.
RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Hr46 receptor represses the early genes activated by the late-larval ecdysone pulse and with the help of Eip75B, provides a temporal linkage between the two ecdysone responses by controlling the expression of ftz-f1.
See Koelle, Seagraves and Hogness, PNAS 89:6167--6171 .
Complementation group identified in an EMS and DEB screen to isolate deficiencies that uncover Jra.