FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\dib
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General Information
Symbol
Dmel\dib
Species
D. melanogaster
Name
disembodied
Annotation Symbol
CG12028
Feature Type
protein_coding_gene
FlyBase ID
FBgn0000449
Gene Model Status
Current
Stock Availability
11 publicly available
Enzyme Name (EC)
3-dehydro-2,22-dideoxyecdysone 22-hydroxylase (1.14.15.44)
Gene Summary
disembodied (dib) encodes a cytochrome P450 involved in ecdysteroid biosynthesis. It shows a mitochondrial localization and catalyzes the addition of a hydroxyl group to the 22 carbon of the cholesterol side chain. dib mutants fail to undergo head involution, dorsal closure and to secrete cuticle. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Also Known As

Cyp302a1, cytochrome P450

Key Links
Genomic Location
Cytogenetic map
64A5-64A5
Sequence location
Recombination map
3-10
RefSeq locus
NT_037436 REGION:4062292..4064551
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (12 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables ecdysteroid 22-hydroxylase activity
inferred from direct assay
(Warren et al., 2002)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
enables heme binding
inferred from electronic annotation with InterPro:IPR001128, InterPro:IPR002401, InterPro:IPR036396
(InterPro Project Members, 2004-)
enables iron ion binding
(InterPro Project Members, 2004-)
enables monooxygenase activity
inferred from electronic annotation with InterPro:IPR001128, InterPro:IPR002401, InterPro:IPR036396
(InterPro Project Members, 2004-)
enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
(InterPro Project Members, 2004-)
Biological Process (6 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
involved_in central nervous system development
inferred from mutant phenotype
(Giesen et al., 2003)
involved_in chitin-based embryonic cuticle biosynthetic process
inferred from mutant phenotype
(Chavez et al., 2000)
involved_in dorsal closure
inferred from mutant phenotype
(Chavez et al., 2000)
involved_in ecdysone biosynthetic process
inferred from direct assay
(Warren et al., 2002)
inferred from mutant phenotype
(Chavez et al., 2000)
involved_in head involution
inferred from mutant phenotype
(Chavez et al., 2000)
involved_in midgut development
inferred from mutant phenotype
(Chavez et al., 2000)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in mitochondrion
inferred from direct assay
(Talamillo et al., 2008, Petryk et al., 2003)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the cytochrome P450 family. (Q9NGX9)
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
ecdysteroid 22-hydroxylase activity
3-dehydro-2,22-dideoxyecdysone + 2 reduced [adrenodoxin] + O2 + 2 H(+) = 3-dehydro-2-deoxyecdysone + 2 oxidized [adrenodoxin] + H2O (1.14.15.44)
RHEA 82055: RHEA 83295: RHEA 83299:
Summaries
Gene Snapshot
disembodied (dib) encodes a cytochrome P450 involved in ecdysteroid biosynthesis. It shows a mitochondrial localization and catalyzes the addition of a hydroxyl group to the 22 carbon of the cholesterol side chain. dib mutants fail to undergo head involution, dorsal closure and to secrete cuticle. [Date last reviewed: 2019-03-07]
Gene Group (FlyBase)
CYTOCHROME P450 - MITO CLAN -
Cytochrome P450, or CYP genes, constitute one of the largest families of genes, with representatives in virtually all living organisms. P450 enzymes are heme-thiolate proteins best known for their monooxygenase role, catalyzing the transfer of one atom of molecular oxygen to a substrate and reducing the other to water. The MITO clan includes D. melanogaster CYP12, 49, 301, 302, 314 and 315 family genes. Members are localized to mitochondria and at least some are linked to the ecdysteroid metabolism pathway. (Adapted from FBrf0248421.)
Protein Function (UniProtKB)
Required for CNS development; negatively regulates glial cell division in the embryonic midline. Involved in the metabolism of insect hormones; responsible for ecdysteroid C22-hydroxylase activity. May be involved in the breakdown of synthetic insecticides.
(UniProt, Q9NGX9)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
dib: disembodied
Homozygous lethal; no differentiation of cuticle and head skeleton.
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\dib for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9NGX9)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.44

Gene model reviewed during 5.46

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
dib-RA
FBtr0073205
NM_080071
1975
489
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
dib-PA
FBpp0073061
55.6
489
9.43
Q9NGX9
NP_524810
AAF47831
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\dib using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.19

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 4
organism | anterior
(Chavez et al., 2000)
embryonic stage 5
organism | posterior | striped
(Chavez et al., 2000)
extended germ band stage
embryonic epidermis
(Chavez et al., 2000)
embryonic stage 11 -- 16
prothoracic gland
(Chung et al., 2009)
embryonic stage 14 -- 17
ring gland & embryonic prothoracic segment
(Chavez et al., 2000)
third instar larval stage
ring gland & prothoracic segment
(Chavez et al., 2000)
prothoracic gland
(Chung et al., 2009)
early third instar larval stage
ring gland
(Ou et al., 2016)
oogenesis stage S8 -- S11 & adult stage | female
follicle cell
(Chavez et al., 2000)
Additional Descriptive Data

dib transcripts are first observed at the early syncytial blastoderm stage where they are enriched in the anterior half of the embryo. As cellularization proceeds, posterior stripes appear and by germ band extension, dib is expressed in most epidermal cells. Expression then fades and by stage 12 is only observed in a small number of unidentified cells per segment. During dorsal closure, precursors of the ring gland express dib in the prothoracic region. Expression remains strong in the prothoracic portion of the ring gland through to stage 17. In wandering third instar larvae, expression is again observed in the prothoracic portion of the ring gland and the expression fades in prepupae. In adult females, expression is observed in follicle cells at stage 8 of oogenesis and is greatly diminished by stage 11.

(Chavez et al., 2000)
Marker for
 
ring gland
(Ou et al., 2016)
Subcellular Localization
CV Term
Polypeptide Expression
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\dib in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 14 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 24 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of dib
Transgenic constructs containing regulatory region of dib
Aberrations (Deficiencies and Duplications) ( 14 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal | recessive
lethal - all die during embryonic stage | recessive
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
Sterility
Allele
female semi-fertile, with Scer\GAL4GMR47A04
fertile
Other Phenotypes
Allele
abnormal developmental rate, with Scer\GAL4phtm.PO
abnormal memory | adult stage | RU486 conditional, with Scer\GAL4αTub84B.Switch.PK
increased body size, with Scer\GAL4phtm.PO
some die during embryonic stage | recessive
wild-type
Phenotype manifest in
Allele
cephalopharyngeal skeleton (with dibF8)
cephalopharyngeal skeleton (with dibP3)
embryo | dorsal closure stage
embryo | dorsal closure stage (with dibF8)
embryo | dorsal closure stage (with dibP3)
embryo | embryonic stage 14 (with dibF8)
embryo | embryonic stage 14 (with dibP3)
embryonic/first instar larval cuticle
embryonic/first instar larval cuticle (with dibF8)
embryonic/first instar larval cuticle (with dibP3)
embryonic/larval foregut
embryonic/larval hindgut
embryonic/larval midgut
embryonic head
embryonic midgut constriction (with dibF8)
embryonic midgut constriction (with dibP3)
embryonic ventral epidermis
glial cell
hindgut proper primordium (with dibF8)
hindgut proper primordium (with dibP3)
larval dorsal hair (with dibF8)
larval dorsal hair (with dibP3)
larval longitudinal connective
larval ventral nerve cord
midgut primordium (with dibF8)
midgut primordium (with dibP3)
midline glial cell | ectopic
mouth hook, with Scer\GAL4phtm.PO
presumptive embryonic/larval central nervous system
pupa, with Scer\GAL4phtm.PO
symmetrical commissure
ventral denticle belt (with dibF8)
ventral denticle belt (with dibP3)
yolk granule (with dibF8)
yolk granule (with dibP3)
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (29)
Hsap\CYP24A1
5 of 14
Yes
No
1  
Hsap\CYP27A1
4 of 14
No
No
Hsap\CYP27B1
4 of 14
No
No
Hsap\CYP27C1
4 of 14
No
No
Hsap\CYP11A1
3 of 14
No
No
Hsap\CYP11B1
3 of 14
No
Yes
Hsap\CYP11B2
3 of 14
No
Yes
Hsap\CYP2E1
1 of 14
No
No
Hsap\CYP2J2
1 of 14
No
No
Hsap\CYP2U1
1 of 14
No
No
Hsap\CYP2W1
1 of 14
No
No
Hsap\CYP3A4
1 of 14
No
No
1  
Hsap\CYP3A5
1 of 14
No
No
Hsap\CYP3A7-CYP3A51P
1 of 14
No
No
Hsap\CYP3A7
1 of 14
No
No
Hsap\CYP3A43
1 of 14
No
No
Hsap\CYP4A11
1 of 14
No
No
Hsap\CYP4B1
1 of 14
No
No
Hsap\CYP4F3
1 of 14
No
No
Hsap\CYP4F8
1 of 14
No
No
Hsap\CYP4F12
1 of 14
No
No
Hsap\CYP4V2
1 of 14
No
No
Hsap\CYP4X1
1 of 14
No
No
Hsap\CYP4Z1
1 of 14
No
No
Hsap\CYP19A1
1 of 14
No
No
Hsap\CYP21A2
1 of 14
No
No
Hsap\CYP26A1
1 of 14
No
No
Hsap\CYP46A1
1 of 14
No
No
Hsap\CYP51A1
1 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (21)
Rnor\Cyp24a1
5 of 14
Yes
No
Rnor\Cyp27a1
4 of 14
No
No
Rnor\Cyp27b1
4 of 14
No
No
Rnor\Cyp11a1
3 of 14
No
No
Rnor\Cyp11b2
3 of 14
No
Yes
Rnor\Cyp11b3
3 of 14
No
Yes
Rnor\Cyp2j3
1 of 14
No
No
Rnor\Cyp2j16
1 of 14
No
No
Rnor\Cyp2s1
1 of 14
No
No
Rnor\Cyp3a2
1 of 14
No
No
Rnor\Cyp3a9
1 of 14
No
No
Rnor\Cyp3a18
1 of 14
No
No
Rnor\Cyp3a23-3a1
1 of 14
No
No
Rnor\Cyp3a62
1 of 14
No
No
Rnor\Cyp4a1
1 of 14
No
No
Rnor\Cyp4b1
1 of 14
No
No
Rnor\Cyp4f5
1 of 14
No
No
Rnor\Cyp4f18
1 of 14
No
No
Rnor\Cyp4v3
1 of 14
No
No
Rnor\Cyp11b1
1 of 14
No
Yes
Rnor\LOC680316
1 of 14
No
Yes
Mus musculus (laboratory mouse) (22)
Mmus\Cyp24a1
5 of 14
Yes
No
Mmus\Cyp11a1
4 of 14
No
No
Mmus\Cyp27b1
4 of 14
No
No
Mmus\Cyp11b1
3 of 14
No
No
Mmus\Cyp27a1
3 of 14
No
No
Mmus\Cyp11b2
2 of 14
No
No
Mmus\Cyp2j7
1 of 14
No
No
Mmus\Cyp2j8
1 of 14
No
No
Mmus\Cyp2j11
1 of 14
No
No
Mmus\Cyp2w1
1 of 14
No
No
Mmus\Cyp3a11
1 of 14
No
No
Mmus\Cyp3a13
1 of 14
No
No
Mmus\Cyp3a16
1 of 14
No
No
Mmus\Cyp3a25
1 of 14
No
No
Mmus\Cyp3a41a
1 of 14
No
No
Mmus\Cyp3a41b
1 of 14
No
No
Mmus\Cyp3a44
1 of 14
No
No
Mmus\Cyp3a57
1 of 14
No
No
Mmus\Cyp3a59
1 of 14
No
No
Mmus\Cyp4f37
1 of 14
No
No
Mmus\Cyp4v3
1 of 14
No
No
Mmus\Cyp17a1
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (30)
Xtro\cyp11a1
4 of 13
Yes
Yes
Xtro\cyp24a1
4 of 13
Yes
No
Xtro\LOC100496967
4 of 13
Yes
No
Xtro\cyp27b1
3 of 13
No
No
Xtro\il17d
3 of 13
No
No
Xtro\LOC101731070
3 of 13
No
Yes
Xtro\XB1001094
3 of 13
No
No
Xtro\cyp27a1.3
2 of 13
No
No
Xtro\LOC116407844
2 of 13
No
No
Xtro\XB984297
2 of 13
No
No
Xtro\cyp1c1
1 of 13
No
No
Xtro\cyp1d1
1 of 13
No
No
Xtro\cyp2a6.1
1 of 13
No
No
Xtro\cyp2a6.3
1 of 13
No
No
Xtro\cyp2a6.8
1 of 13
No
No
Xtro\cyp2a6.9
1 of 13
No
No
Xtro\cyp2c8l
1 of 13
No
No
Xtro\cyp26a1
1 of 13
No
No
Xtro\cyp27a1
1 of 13
No
No
Xtro\cyp27c1
1 of 13
No
No
Xtro\cyp46a1.3
1 of 13
No
No
Xtro\LOC100491603
1 of 13
No
No
Xtro\LOC100491773
1 of 13
No
No
Xtro\LOC100497899
1 of 13
No
No
Xtro\LOC100498621
1 of 13
No
No
Xtro\rem2
1 of 13
No
No
Xtro\XB980013
1 of 13
No
No
Xtro\XB5810926
1 of 13
No
No
Xtro\XB5880541
1 of 13
No
No
Xtro\XB5919979
1 of 13
No
No
Danio rerio (Zebrafish) (24)
Drer\cyp24a1
4 of 14
Yes
No
Drer\cyp27a1.2
4 of 14
Yes
No
Drer\cyp27a1.4
4 of 14
Yes
No
Drer\cyp27a7
4 of 14
Yes
No
Drer\cyp27c1
4 of 14
Yes
No
Drer\si:ch211-113j14.1
4 of 14
Yes
No
Drer\si:dkey-91i10.3
4 of 14
Yes
No
Drer\zgc:136333
4 of 14
Yes
No
Drer\cyp11a1
3 of 14
No
No
Drer\cyp11a2
3 of 14
No
No
Drer\cyp11c1
3 of 14
No
Yes
Drer\cyp27b1
2 of 14
No
No
Drer\cyp2n13
1 of 14
No
No
Drer\cyp2p10
1 of 14
No
No
Drer\cyp2v1
1 of 14
No
No
Drer\cyp3a65
1 of 14
No
No
Drer\cyp3c1
1 of 14
No
No
Drer\cyp3c3
1 of 14
No
No
Drer\cyp4v7
1 of 14
No
No
Drer\cyp4v8
1 of 14
No
No
Drer\cyp46a1.2
1 of 14
No
No
Drer\cyp46a1.4
1 of 14
No
No
Drer\tbxas1
1 of 14
No
No
Drer\zgc:123299
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (33)
Cele\cyp-44A1
5 of 14
Yes
No
Cele\cyp-13A1
1 of 14
No
No
Cele\cyp-13A2
1 of 14
No
No
Cele\cyp-13A3
1 of 14
No
No
Cele\cyp-13A5
1 of 14
No
No
Cele\cyp-13A6
1 of 14
No
No
Cele\cyp-13A7
1 of 14
No
No
Cele\cyp-13A10
1 of 14
No
No
Cele\cyp-13A11
1 of 14
No
No
Cele\cyp-13A12
1 of 14
No
No
Cele\cyp-13B1
1 of 14
No
No
Cele\cyp-13B2
1 of 14
No
No
Cele\cyp-25A1
1 of 14
No
No
Cele\cyp-25A2
1 of 14
No
No
Cele\cyp-25A3
1 of 14
No
No
Cele\cyp-25A4
1 of 14
No
No
Cele\cyp-29A2
1 of 14
No
No
Cele\cyp-29A3
1 of 14
No
No
Cele\cyp-29A4
1 of 14
No
No
Cele\cyp-31A2
1 of 14
No
No
Cele\cyp-31A3
1 of 14
No
No
Cele\cyp-32A1
1 of 14
No
No
Cele\cyp-32B1
1 of 14
No
No
Cele\cyp-33C2
1 of 14
No
No
Cele\cyp-33E1
1 of 14
No
No
Cele\cyp-33E2
1 of 14
No
No
Cele\cyp-34A5
1 of 14
No
No
Cele\cyp-34A6
1 of 14
No
No
Cele\cyp-34A7
1 of 14
No
No
Cele\cyp-37A1
1 of 14
No
No
Cele\cyp-37B1
1 of 14
No
No
Cele\cyp-42A1
1 of 14
No
No
Cele\npp-24
1 of 14
No
Yes
Anopheles gambiae (African malaria mosquito) (65)
Agam\AgaP_AGAP005992
12 of 12
Yes
Yes
Agam\AgaP_AGAP005774
6 of 12
No
No
Agam\AgaP_AGAP006082
6 of 12
No
No
Agam\AgaP_AGAP008018
5 of 12
No
No
Agam\AgaP_AGAP008019
5 of 12
No
No
Agam\AgaP_AGAP008020
5 of 12
No
No
Agam\AgaP_AGAP008022
5 of 12
No
No
Agam\AgaP_AGAP012800
5 of 12
No
No
Agam\AgaP_AGAP002429
4 of 12
No
No
Agam\AgaP_AGAP000284
3 of 12
No
No
Agam\AgaP_AGAP008021
3 of 12
No
No
Agam\AgaP_AGAP000088
1 of 12
No
No
Agam\AgaP_AGAP000192
1 of 12
No
No
Agam\AgaP_AGAP000193
1 of 12
No
No
Agam\AgaP_AGAP000194
1 of 12
No
No
Agam\AgaP_AGAP001076
1 of 12
No
No
Agam\AgaP_AGAP001443
1 of 12
No
No
Agam\AgaP_AGAP001861
1 of 12
No
No
Agam\AgaP_AGAP001864
1 of 12
No
No
Agam\AgaP_AGAP002195
1 of 12
No
No
Agam\AgaP_AGAP002197
1 of 12
No
No
Agam\AgaP_AGAP002204
1 of 12
No
No
Agam\AgaP_AGAP002205
1 of 12
No
No
Agam\AgaP_AGAP002206
1 of 12
No
No
Agam\AgaP_AGAP002208
1 of 12
No
No
Agam\AgaP_AGAP002209
1 of 12
No
No
Agam\AgaP_AGAP002210
1 of 12
No
No
Agam\AgaP_AGAP002211
1 of 12
No
No
Agam\AgaP_AGAP002416
1 of 12
No
No
Agam\AgaP_AGAP002417
1 of 12
No
No
Agam\AgaP_AGAP002418
1 of 12
No
No
Agam\AgaP_AGAP002419
1 of 12
No
No
Agam\AgaP_AGAP002555
1 of 12
No
No
Agam\AgaP_AGAP002865
1 of 12
No
No
Agam\AgaP_AGAP002866
1 of 12
No
No
Agam\AgaP_AGAP002868
1 of 12
No
No
Agam\AgaP_AGAP002870
1 of 12
No
No
Agam\AgaP_AGAP003343
1 of 12
No
No
Agam\AgaP_AGAP003608
1 of 12
No
No
Agam\AgaP_AGAP006047
1 of 12
No
No
Agam\AgaP_AGAP006049
1 of 12
No
No
Agam\AgaP_AGAP007480
1 of 12
No
No
Agam\AgaP_AGAP008204
1 of 12
No
No
Agam\AgaP_AGAP008205
1 of 12
No
No
Agam\AgaP_AGAP008208
1 of 12
No
No
Agam\AgaP_AGAP008209
1 of 12
No
No
Agam\AgaP_AGAP008212
1 of 12
No
No
Agam\AgaP_AGAP008214
1 of 12
No
No
Agam\AgaP_AGAP008217
1 of 12
No
No
Agam\AgaP_AGAP008356
1 of 12
No
No
Agam\AgaP_AGAP008358
1 of 12
No
No
Agam\AgaP_AGAP008552
1 of 12
No
No
Agam\AgaP_AGAP009240
1 of 12
No
No
Agam\AgaP_AGAP009241
1 of 12
No
No
Agam\AgaP_AGAP009246
1 of 12
No
No
Agam\AgaP_AGAP010414
1 of 12
No
No
Agam\AgaP_AGAP012292
1 of 12
No
No
Agam\AgaP_AGAP012957
1 of 12
No
No
Agam\AgaP_AGAP013224
1 of 12
No
No
Agam\AgaP_AGAP013241
1 of 12
No
No
Agam\AgaP_AGAP013305
1 of 12
No
No
Agam\AgaP_AGAP013490
1 of 12
No
No
Agam\CYP6P4
1 of 12
No
No
Agam\CYP6Z2
1 of 12
No
No
Agam\CYP9L1
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (63)
Atha\AT2G12190
1 of 13
Yes
No
Atha\BAS1
1 of 13
Yes
No
Atha\CYP71A13
1 of 13
Yes
No
Atha\CYP71A15
1 of 13
Yes
No
Atha\CYP71A18
1 of 13
Yes
No
Atha\CYP71A19
1 of 13
Yes
No
Atha\CYP71A20
1 of 13
Yes
No
Atha\CYP71A23
1 of 13
Yes
No
Atha\CYP71A25
1 of 13
Yes
No
Atha\CYP71A26
1 of 13
Yes
No
Atha\CYP71B4
1 of 13
Yes
No
Atha\CYP71B8
1 of 13
Yes
No
Atha\CYP71B13
1 of 13
Yes
No
Atha\CYP71B14
1 of 13
Yes
No
Atha\CYP71B21
1 of 13
Yes
No
Atha\CYP71B24
1 of 13
Yes
No
Atha\CYP71B25
1 of 13
Yes
No
Atha\CYP71B31
1 of 13
Yes
No
Atha\CYP71B34
1 of 13
Yes
No
Atha\CYP71B35
1 of 13
Yes
No
Atha\CYP71B36
1 of 13
Yes
No
Atha\CYP76C1
1 of 13
Yes
No
Atha\CYP76C3
1 of 13
Yes
No
Atha\CYP76C5
1 of 13
Yes
No
Atha\CYP76C7
1 of 13
Yes
No
Atha\CYP79A2
1 of 13
Yes
No
Atha\CYP81D4
1 of 13
Yes
No
Atha\CYP81D7
1 of 13
Yes
No
Atha\CYP81D11
1 of 13
Yes
No
Atha\CYP81F3
1 of 13
Yes
No
Atha\CYP81F4
1 of 13
Yes
No
Atha\CYP81H1
1 of 13
Yes
No
Atha\CYP82C2
1 of 13
Yes
No
Atha\CYP82C4
1 of 13
Yes
No
Atha\CYP82G1
1 of 13
Yes
No
Atha\CYP83A1
1 of 13
Yes
No
Atha\CYP86B1
1 of 13
Yes
No
Atha\CYP91A2
1 of 13
Yes
No
Atha\CYP93D1
1 of 13
Yes
No
Atha\CYP96A1
1 of 13
Yes
No
Atha\CYP96A4
1 of 13
Yes
No
Atha\CYP96A10
1 of 13
Yes
No
Atha\CYP97A3
1 of 13
Yes
No
Atha\CYP97B3
1 of 13
Yes
No
Atha\CYP703A2
1 of 13
Yes
No
Atha\CYP704A1
1 of 13
Yes
No
Atha\CYP704A2
1 of 13
Yes
No
Atha\CYP705A13
1 of 13
Yes
No
Atha\CYP705A18
1 of 13
Yes
No
Atha\CYP705A20
1 of 13
Yes
No
Atha\CYP705A23
1 of 13
Yes
No
Atha\CYP705A27
1 of 13
Yes
No
Atha\CYP705A28
1 of 13
Yes
No
Atha\CYP705A30
1 of 13
Yes
No
Atha\CYP706A3
1 of 13
Yes
No
Atha\CYP706A6
1 of 13
Yes
No
Atha\CYP709B3
1 of 13
Yes
No
Atha\CYP711A1
1 of 13
Yes
No
Atha\CYP712A2
1 of 13
Yes
No
Atha\CYP714A2
1 of 13
Yes
No
Atha\GA3
1 of 13
Yes
Yes
Atha\LUT1
1 of 13
Yes
No
Atha\TT7
1 of 13
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\DIT2
1 of 13
Yes
No
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:dib. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (57)
Cyp49a1
7 of 13
Cyp301a1
7 of 13
Cyp12a4
6 of 13
Cyp12a5
6 of 13
Cyp12b2
6 of 13
Cyp12c1
6 of 13
Cyp12d1-d
6 of 13
Cyp12d1-p
6 of 13
Cyp12e1
6 of 13
shd
5 of 13
sad
3 of 13
Cyp313a1
2 of 13
Cyp4aa1
1 of 13
Cyp4ac1
1 of 13
Cyp4ac2
1 of 13
Cyp4ac3
1 of 13
Cyp4ad1
1 of 13
Cyp4ae1
1 of 13
Cyp4c3
1 of 13
Cyp4d1
1 of 13
Cyp4d2
1 of 13
Cyp4d8
1 of 13
Cyp4d14
1 of 13
Cyp4d20
1 of 13
Cyp4d21
1 of 13
Cyp4e1
1 of 13
Cyp4e2
1 of 13
Cyp4e3
1 of 13
Cyp4g1
1 of 13
Cyp4g15
1 of 13
Cyp4p1
1 of 13
Cyp4p3
1 of 13
Cyp4s3
1 of 13
Cyp6a2
1 of 13
Cyp6a8
1 of 13
Cyp6a13
1 of 13
Cyp6a14
1 of 13
Cyp6a17
1 of 13
Cyp6a18
1 of 13
Cyp6a19
1 of 13
Cyp6a20
1 of 13
Cyp6a22
1 of 13
Cyp6d4
1 of 13
Cyp6d5
1 of 13
Cyp6g1
1 of 13
Cyp18a1
1 of 13
Cyp28a5
1 of 13
Cyp28d1
1 of 13
Cyp28d2
1 of 13
Cyp311a1
1 of 13
Cyp312a1
1 of 13
Cyp313a2
1 of 13
Cyp313a3
1 of 13
Cyp313a4
1 of 13
Cyp313a5
1 of 13
Cyp313b1
1 of 13
Cyp318a1
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 4 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    CYP24A1; cytochrome P450 family 24 subfamily A member 1
    5 of 14
    126065
     
    CYP27A1; cytochrome P450 family 27 subfamily A member 1
    4 of 14
    606530
    CYP27B1; cytochrome P450 family 27 subfamily B member 1
    4 of 14
    609506
    CYP27C1; cytochrome P450 family 27 subfamily C member 1
    4 of 14
    620605
        CYP11A1; cytochrome P450 family 11 subfamily A member 1
        3 of 14
        118485
        CYP11B1; cytochrome P450 family 11 subfamily B member 1
        3 of 14
        610613
        CYP11B2; cytochrome P450 family 11 subfamily B member 2
        3 of 14
        124080
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Interaction Browsers
        Summary of Genetic Interactions
        Interaction Browsers
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        FlyBase
        External Links
        KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        External Data
        Linkouts
        KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        Class of Gene
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        3L
        Recombination map
        3-10
        Cytogenetic map
        64A5-64A5
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        64A5-64A5
        Limits computationally determined from genome sequence between P{PZ}l(3)rG166rG166 and P{PZ}sinu06524
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        64A5-64A5
        (Dunkov, 2000.6.29)
        Experimentally Determined Recombination Data
        Location

        3-10

        (FlyBase, 2016)

        3-8.6

        (Comeron et al., 2012)

        3-12

        (Tearle and Nusslein-Volhard, 1987)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (11)
        Genomic Clones (17)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (4)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            Antibody Information
            Laboratory Generated Antibodies
             

            polyclonal

            (Simon et al., 2025)
            Commercially Available Antibodies
             
            Cell Line Information
            Publicly Available Cell Lines
             
              Other Stable Cell Lines
               
                Other Comments

                dib encodes a 22-hydroxylase that catalyses the conversion of 2,22-dideoxyecdysone (ketotriol) to 2-deoxyecdysone in S2 cells.

                (Warren et al., 2002)

                Mutant embryos produce little or no cuticle and show severe defects in many late morphogenetic processes such as head involution, dorsal closure and gut development.

                (Chavez et al., 2000)

                Four EMS induced alleles have been identified in a screen for mutations affecting commissure formation in the CNS of the embryo.

                (Hummel et al., 1999)

                Discussed during genetic analysis of the Ras64B-Rop region.

                (Harrison et al., 1995)

                dib mutants display no differentiation of the cuticle or the head skeleton.

                (Jurgens et al., 1984)
                Relationship to Other Genes
                Source for database merge of

                Source for merge of: dib CG12028

                (FlyBase, 1996-)
                Additional comments

                Source for merge of dib CG12028 was sequence comparison ( date:000401 ).

                (FlyBase, 1996-)
                Nomenclature History
                Source for database identify of
                Nomenclature comments
                Etymology
                Synonyms and Secondary IDs (15)
                Reported As
                Symbol Synonym
                302a1
                (Nelson, 2001.2.5)
                CG12028
                (Kim et al., 2018, Keleman et al., 2009.8.5, Whitfield, 2000.9.1)
                CYP302A1
                (Chen and Li, 2007, Rewitz et al., 2007, Ranson et al., 2002, Tijet et al., 2001, Dunkov, 2000.10.12)
                Cyp
                (Gim et al., 2001)
                Cyp27
                (Kim et al., 2018)
                Cyp302a1
                (Scanlan et al., 2020, Seong et al., 2018, Komura-Kawa et al., 2015, Good et al., 2014, Niwa et al., 2011, Rewitz et al., 2007, Kasai and Tomita, 2003)
                Dib
                (Ostalé et al., 2024, Yuan et al., 2024, Wen et al., 2023, Kamiyama and Niwa, 2022, Pan et al., 2020, Liu et al., 2018, Valzania et al., 2014, Talamillo et al., 2013)
                DmCYP302A1
                (Lin et al., 2023)
                dib
                (Liu et al., 2025, Liu et al., 2025, Liu et al., 2024, Frat et al., 2023, Carlson et al., 2022, Gupta et al., 2022, Ohhara et al., 2022, Heredia et al., 2021, Kannangara et al., 2021, Keith et al., 2021, Pan and O'Connor, 2021, Sood et al., 2021, Yoo et al., 2021, Zhao et al., 2021, Ahmed et al., 2020, Huynh et al., 2020, Huynh et al., 2020, Kannangara et al., 2020, Koyama et al., 2020, Scanlan et al., 2020, Zeng et al., 2020, Haines and Eisen, 2018, Huynh et al., 2018, Ignesti et al., 2018, Setiawan et al., 2018, Shimell et al., 2018, Uryu et al., 2018, Zeng et al., 2018, Zhang et al., 2018, Christesen et al., 2017, Transgenic RNAi Project members, 2017-, Di Cara and King-Jones, 2016, Valzania et al., 2016, Borsos et al., 2015, Deady et al., 2015, Jaszczak et al., 2015, Kok et al., 2015, Komura-Kawa et al., 2015, Xie et al., 2015, Bischof and FlyORF project members, 2014.6.20, Danielsen et al., 2014, Good et al., 2014, Koyama et al., 2014, Neuman et al., 2014, Deng and Kerppola, 2013, Koyama et al., 2013, Colombani et al., 2012, Garelli et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Nfonsam et al., 2012, Vamos and Boros, 2012, Guittard et al., 2011, Niwa et al., 2011, Ou et al., 2011, Wang et al., 2011, Beller et al., 2010, Pankotai et al., 2010, Ruaud et al., 2010, Xiang et al., 2010, Reuveny et al., 2009, Walkiewicz and Stern, 2009, Huang et al., 2008, Layalle et al., 2008, Talamillo et al., 2008, McBrayer et al., 2007, Ono et al., 2006, Palanker et al., 2006, Warren et al., 2006, Terashima and Bownes, 2005, Giesen et al., 2003)
                l(3)64Ak
                (Harrison et al., 1995)
                l(3)SH14
                (Harrison et al., 1995)
                l(3)dib
                (Vaskova et al., 2000)
                Name Synonyms
                Disembodied
                (Truman and Riddiford, 2023, Jayakumar et al., 2016, Niwa and Niwa, 2016, Pahi et al., 2015, Valzania et al., 2014, Talamillo et al., 2007)
                cytochrome P450
                (Chung et al., 2009, Gim et al., 2001)
                disembodied
                (Wang et al., 2025, Chan et al., 2023, Christensen et al., 2020, Di Cara and King-Jones, 2016, Ou et al., 2016, Wieschaus and Nüsslein-Volhard, 2016, Borsos et al., 2015, Jaszczak et al., 2015, Koyama et al., 2014, Ishimoto et al., 2013, Koyama et al., 2013, Garelli et al., 2012, Gáliková et al., 2011, Guittard et al., 2011, Ou et al., 2011, Beller et al., 2010, Pankotai et al., 2010, Ruaud et al., 2010, Reuveny et al., 2009, Walkiewicz and Stern, 2009, Huang et al., 2008, Layalle et al., 2008, Talamillo et al., 2008, McBrayer et al., 2007, Rewitz et al., 2007, Palanker et al., 2006, Warren et al., 2006, Terashima and Bownes, 2005, Giesen et al., 2003, Hummel et al., 1999)
                Secondary FlyBase IDs
                • FBgn0035492
                Datasets (0)
                Study focus (0)
                Experimental Role
                Project
                Project Type
                Title
                Study result (0)
                Result
                Result Type
                Title
                External Crossreferences and Linkouts ( 30 )
                Sequence Crossreferences
                NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                Other crossreferences
                AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                FlyMine - An integrated database for Drosophila genomics
                InterPro - A database of protein families, domains and functional sites
                KEGG Genes - Molecular building blocks of life in the genomic space.
                MARRVEL_MODEL - MARRVEL (model organism gene)
                Linkouts
                Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                DroID - A comprehensive database of gene and protein interactions.
                DRSC - Results frm RNAi screens
                Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                Flygut - An atlas of the Drosophila adult midgut
                iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                References (193)