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General Information
Symbol
Dmel\dor
Species
D. melanogaster
Name
deep orange
Annotation Symbol
CG3093
Feature Type
FlyBase ID
FBgn0000482
Gene Model Status
Stock Availability
Gene Summary
Plays a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes probably as part of the class C core vacuole/endosome tethering (CORVET) complex (PubMed:9065698, PubMed:10549280, PubMed:27253064, PubMed:25422373). In larval neuromuscular junctions, essential for endosomal sorting which traffics old or dysfunctional synaptic vesicle proteins through a degradative endolysosomal route (PubMed:25422373). Required for the biogenesis of eye pigment granules (PubMed:10549280). Required to maintain normal levels of rush, which functions in endosome formation and trafficking (PubMed:22160599). (UniProt, Q24314)
Contribute a Gene Snapshot for this gene.
Also Known As

Vps18, EG:171E4.1 , Dof, l(1)7

Key Links
Genomic Location
Cytogenetic map
Sequence location
X:1,665,052..1,668,451 [+]
Recombination map
1-0.2
RefSeq locus
NC_004354 REGION:1665052..1668451
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (35 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from physical interaction with UniProtKB:A4V4S5,UniProtKB:M9PF72,UniProtKB:Q9VHG1,UniProtKB:Q9VRX2
(assigned by UniProt )
inferred from physical interaction with UniProtKB:Q9V3I2
(assigned by UniProt )
inferred from physical interaction with UniProtKB:Q9VRX2
(assigned by UniProt )
inferred from physical interaction with UniProtKB:Q9Y1I2
(assigned by UniProt )
contributes_to syntaxin binding
inferred from physical interaction with FLYBASE:Syx17; FB:FBgn0035540
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000595174
(assigned by GO_Central )
inferred from sequence or structural similarity with UniProtKB:Q9P253
inferred from sequence model
Biological Process (25 terms)
Terms Based on Experimental Evidence (18 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
involved_in cell division
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
involved_in endocytosis
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
Terms Based on Predictions or Assertions (9 terms)
CV Term
Evidence
References
inferred from sequence or structural similarity with SGD:S000004138
inferred from biological aspect of ancestor with PANTHER:PTN000595174
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001154188
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001154188
(assigned by GO_Central )
involved_in organelle fusion
inferred from biological aspect of ancestor with PANTHER:PTN000595174
(assigned by GO_Central )
inferred from sequence or structural similarity with UniProtKB:Q9P253
inferred from sequence or structural similarity with SGD:S000004138
inferred from biological aspect of ancestor with PANTHER:PTN000595174
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000595174
(assigned by GO_Central )
Cellular Component (5 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
located_in endosome
inferred from direct assay
part_of HOPS complex
inferred from mutant phenotype
inferred from direct assay
located_in lysosome
inferred from direct assay
(assigned by UniProt )
located_in synapse
inferred from mutant phenotype
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from sequence or structural similarity with SGD:S000004138
is_active_in endosome
inferred from biological aspect of ancestor with PANTHER:PTN000595174
(assigned by GO_Central )
part_of HOPS complex
inferred from sequence or structural similarity with SGD:S000004138
inferred from biological aspect of ancestor with PANTHER:PTN000595174
(assigned by GO_Central )
Protein Family (UniProt)
Belongs to the VPS18 family. (Q24314)
Summaries
Gene Group (FlyBase)
VPS-C COMPLEXES: HOPS AND CORVET -
The heterohexameric VPS-C complexes, HOPS and CORVET have been extensively characterised in yeast. They interact with SNAREs and Rabs to promote vesicle tethering. The CORVET complex is involved in early endosome fusion, interacting with Rab5-GTP. The HOPS complex interacts with Rab7-GTP on late endosomes (multivesicular bodies) to promote fusion with the lysosomal compartment (and vacuoles) and also autophagosome-lysosome fusion. The VPS-C complexes share common subunits and CORVET to HOPS conversion intermediates (i-CORVET and i-HOPS) have been proposed to accompany early to late endosome maturation. (Adapted from PMID:19575650 and FBrf0223135).
OTHER RING DOMAIN UBIQUITIN LIGASES -
The RING (Really Interesting New Gene) finger domain co-ordinates two Zn2+ ions via conserved Cys and His residues. Most proteins which contain a RING finger are assumed to be ubiquitin (E3) ligases. The RING finger binds ubiquitin-conjugating enzymes (E2) and promotes direct transfer of ubiquitin to the target protein. (Adapted from FBrf0215242 and PMID:19489725).
Protein Function (UniProtKB)
Plays a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes probably as part of the class C core vacuole/endosome tethering (CORVET) complex (PubMed:9065698, PubMed:10549280, PubMed:27253064, PubMed:25422373). In larval neuromuscular junctions, essential for endosomal sorting which traffics old or dysfunctional synaptic vesicle proteins through a degradative endolysosomal route (PubMed:25422373). Required for the biogenesis of eye pigment granules (PubMed:10549280). Required to maintain normal levels of rush, which functions in endosome formation and trafficking (PubMed:22160599).
(UniProt, Q24314)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
dor: deep orange
dor mutants affect a number of developmental processes; severity of effect increases with increasing developmental temperature. Eye color orange, shade depending on allele and temperature. dor reduces eye pigmentation in combination with either cn or v or with bw, indicating reduction in both drosopterins and xanthommatin. Biochemical analyses show xanthommatin and five drosopterins to be reduced to different degrees in dor; levels, but not relative proportions, change according to temperature of development (Counce, 1957, Experientia 13: 354; Puckett and Petty, 1980, Biochem. Genet. 18: 1221-28). Reciprocal transplantation experiments show that eye color is autonomous (Hadorn and Counce). dor females produce no progeny in crosses to dor males at 25, although some allelic combinations able to produce progeny at 18; and a few dor/+ daughters are produced in crosses to + males. Germ-line clones homozygous for the lethal allele dor28 produce collapsed eggs (Perriman, Egstrom, and Mahowald, 1989, Genetics 121: 333-52). The lethal embryos produced by dor mothers reach gastrulation or beyond (Hildreth and Lucchesi, 1967, Dev. Biol. 15: 536-52; Counce, 1969, DIS 44: 101-82). Maternal effect shown to be germ line autonomous by both ovarian (Garen and Gehring, 1972, Proc. Nat. Acad. Sci. USA 69: 2982-85) and pole-cell transplantation (Marsh, van Deusen, Wieschaus, and Gehring, 1977, Dev. Biol. 56: 195-99). Maternal lethal effect rescuable by injections into preblastoderm embryos of cytoplasm from unfertilized eggs of normal females (Garen and Gehring, 1972); dor+ substance present during early stages of vitellogenesis but not detected in yolk of cellular blastoderm embryos (Marsh et al., 1977). Abnormalities of dor cells in culture eliminated by extracts of normal post- but not pregastrulation embryos (Kuroda, 1977, Dev. Growth Differ. 19: 57-66). dor males show variable extents of gonadal dysgenesis depending on culture conditions and genotypic background; abnormalities range from failure of testes to attach to genital ducts to failure of one attached testis to elongate (Lucchesi, Counce, and Hildreth, 1968, J. Exp. Zool. 168: 437-50). Viability and longevity of dor homozygotes and hemizygotes variably reduced depending on allele and temperature; dorl larvae develop melanotic pseudo-tumors (Stark, 1918, J. Exp. Zool. 27: 509-29; Oftedal, 1953, Z. Indukt. Abstamm. Vererbungsl. 85: 408-22) and midgut occlusion (Russell, 1940, J. Exp. Zool. 84: 363-79), dying in late third instar (Bischoff and Lucchesi, 1971). dor/dorl lethal at 29 (Belyaeva, Aizenzon, Semeshin, Kiss, Koczka, Baritcheva, Gorelova, and Zhimulev, 1980, Chromosoma 81: 281-306). dor in combination with ry, ry2 (Lucchesi, 1968, Genetics 59: 37-44) and car (Nash, 1971, DIS 47: 73) causes lethality in pupal stage. Recovery of gynandromorphs with dor car male sectors less than in controls; bilateral gynandromorphs not observed, but distribution of male tissue resembles that of control (Grell, 1976). dor behaves as a semilethal in combination with pd and with cn bw (Lucchesi, 1968).
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\dor for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.42

Gene model reviewed during 5.39

Gene model reviewed during 5.45

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0070269
3161
1002
Additional Transcript Data and Comments
Reported size (kB)

3.1 (northern blot)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0070259
115.3
1002
5.52
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

115 (kD observed)

1002 (aa); 115.3 (kD predicted)

Comments
External Data
Subunit Structure (UniProtKB)

Component of the class C core vacuole/endosome tethering (CORVET) complex composed of at least dor/Vps18, Vps16A, Vps8 and car/Vps33A; unlike in other species, Vps11 is not part of the Drosophila complex (PubMed:27253064). Interacts with car (PubMed:10549280). Interacts with ema (PubMed:20194640).

(UniProt, Q24314)
Crossreferences
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\dor using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

dor protein colocalizes with internalized dextran beads for a limited time in endocytic compartments in third larval instar garland cells. dor protein partially colocalizes with hk protein and with internalized dextran beads to endosomes in Schneider2:Sev cells. hk protein is confined to the perimeter of the large endosomes while dor protein is found in the interior as well.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
located_in endosome
inferred from direct assay
part_of HOPS complex
inferred from mutant phenotype
inferred from direct assay
located_in lysosome
inferred from direct assay
(assigned by UniProt )
located_in synapse
inferred from mutant phenotype
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\dor in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
EMBL-EBI Single Cell Expression Atlas
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 41 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 14 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of dor
Transgenic constructs containing regulatory region of dor
Aberrations (Deficiencies and Duplications) ( 55 )
Inferred from experimentation ( 55 )
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
thorax & macrochaeta | conditional cs
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
15 of 15
Yes
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (1)
13 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (1)
9 of 12
Yes
Yes
Danio rerio (Zebrafish) (1)
14 of 15
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
14 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (1)
8 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
13 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
11 of 12
Yes
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091902FX )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091501O7 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W01AT )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X018T )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G01XI )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (1)
2 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 3 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 4 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Subunit Structure (UniProtKB)
Component of the class C core vacuole/endosome tethering (CORVET) complex composed of at least dor/Vps18, Vps16A, Vps8 and car/Vps33A; unlike in other species, Vps11 is not part of the Drosophila complex (PubMed:27253064). Interacts with car (PubMed:10549280). Interacts with ema (PubMed:20194640).
(UniProt, Q24314 )
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
External Data
Linkouts
Genomic Location and Detailed Mapping Data
Chromosome (arm)
X
Recombination map
1-0.2
Cytogenetic map
Sequence location
X:1,665,052..1,668,451 [+]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
2B5-2B5
Limits computationally determined from genome sequence between P{EP}brEP1515 and P{EP}EP1444&P{EP}CG14818EP1190
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location
Notes

dor has been mapped to the right of su(wa) using a duplication that covers su(wa).

Stocks and Reagents
Stocks (19)
Genomic Clones (10)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (45)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
RNAi and Array Information
Linkouts
DRSC - Results frm RNAi screens
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Laboratory Generated Antibodies
Commercially Available Antibodies
 
Other Information
Relationship to Other Genes
Source for database identify of
Source for database merge of
Additional comments

Mutants can be arranged in a linear sequence of increasing strengths; heterozygotes between different pairs of alleles show intermediate phenotypes; no evidence of interallelic complementation (Bischoff and Lucchesi, 1971). Recombinational mapping by Bischoff (1973) established the order illustrated in Lindsley and Zimm (1992) p.164.

Other Comments

dsRNA has been made from templates generated with primers directed against this gene.

dsRNA made from templates generated with primers directed against dor that is transfected into S2 treated with Listeria monocytogenes reveals a vacuolar escape phenotype in which the bacterial protein listeriolysin O is not required.

dor is required for normal labels of degradation of hh and ptc proteins in the wing disc, but hh signalling is unaffected by this.

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

dor mutations alter trafficking to lysosomes.

dor is part of a protein complex which includes car.

Molecular characterisation of dor.

Localisation of hfw, dor and br are clustered in the X chromosomes of taxonomically distant Drosophila species, suggesting an evolutionary significance of such an organisation.

dor has been located within a puff on the telomeric chromosome of D.funebris, D.virilis, D.hydei, D.repleta, D.mercatorum and D.paranaensis, within a puff on the distal part of the X chromosome of D.kanekoi and within a puff on the proximal portion of the X chromosome of D.pseudoobscura.pseudoobscura.

Lesions in dor reduce or eliminate pigmentation in the eyes and ocelli and block pigmentation of the fat body and tubules: dor is required for normal pigmentation of all four tissues.

The dor locus is subject to T(1;2)dorvar7 position effect variegation.

Lethal dor allele belongs to the class of ecdysone deficient mutations.

dor mutants affect a number of developmental processes; severity of effect increases with increasing developmental temperature. Eye color orange, shade depending on allele and temperature. dor reduces eye pigmentation in combination with either cn or v or with bw, indicating reduction in both drosopterins and xanthommatin. Biochemical analyses show xanthommatin and five drosopterins to be reduced to different degrees in dor; levels, but not relative proportions, change according to temperature of development (Counce, 1957) (Puckett and Petty, 1980). Reciprocal transplantation experiments show that eye color is autonomous (Hadorn and Counce). dor females produce no progeny in crosses to dor males at 25oC, although some allelic combinations able to produce progeny at 18oC; and a few dor/+ daughters are produced in crosses to + males. Germ-line clones homozygous for the lethal allele dor28 produce collapsed eggs (Perriman, Egstrom and Mahowald, 1989). The lethal embryos produced by dor mothers reach gastrulation or beyond (Hildreth and Lucchesi, 1967; Counce, 1969). Maternal effect shown to be germ line autonomous by both ovarian (Garen and Gehring, 1972) and pole-cell transplantation (Marsh, van Deusen, Wieschaus and Gehring, 1977). Maternal lethal effect rescuable by injections into preblastoderm embryos of cytoplasm from unfertilized eggs of normal females (Garen and Gehring, 1972); dor+ substance present during early stages of vitellogenesis but not detected in yolk of cellular blastoderm embryos (Marsh et al., 1977). Abnormalities of dor cells in culture eliminated by extracts of normal post- but not pregastrulation embryos (Kuroda, 1977). dor males show variable extents of gonadal dysgenesis depending on culture conditions and genotypic background; abnormalities range from failure of testes to attach to genital ducts to failure of one attached testis to elongate (Lucchesi, Counce and Hildreth, 1968). Viability and longevity of dor homozygotes and hemizygotes variably reduced depending on allele and temperature; dor8 larvae develop melanotic pseudotumors (Stark, 1918; Oftedal, 1953) and midgut occlusion (Russell, 1940), dying in late third instar (Bischoff and Lucchesi, 1971). dor/dor8 lethal at 29oC (Belyaeva, Aizenzon, Semeshin, Kiss, Koczka, Baritcheva, Gorelova, and Zhimulev, 1980). dor in combination with ry, ry2 (Lucchesi, 1968) and car (Nash, 1971) causes lethality in pupal stage. Recovery of gynandromorphs with dor car male sectors less than in controls; bilateral gynandromorphs not observed, but distribution of male tissue resembles that of control (Grell, 1976). dor behaves as a semi-lethal in combination with pd and with cn bw (Lucchesi, 1968).

Origin and Etymology
Discoverer

E.D. King.

Etymology
Identification
External Crossreferences and Linkouts ( 40 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
Other crossreferences
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
EMBL-EBI Single Cell Expression Atlas
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
KEGG Genes - Molecular building blocks of life in the genomic space.
MARRVEL_MODEL
modMine - A data warehouse for the modENCODE project
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
DRSC - Results frm RNAi screens
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
FlyMine - An integrated database for Drosophila genomics
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Synonyms and Secondary IDs (18)
Reported As
Symbol Synonym
l(1)2Be
l(1)2Bw
l(1)76
Secondary FlyBase IDs
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