Disheveled, Dvl, l(1)G0267, l(1)10Bd
Gene model reviewed during 5.45
Gene model reviewed during 5.50
There is only one protein coding transcript and one polypeptide associated with this gene
Interacts with nkd. This interaction may require zinc.
Phosphorylated. Wg signaling generates the hyperphosphorylated active forms.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\dsh using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\dsh in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: dsh CG1720
Source for merge of dsh CG18361 was sequence comparison ( date:001104 ).
dsRNA made from templates generated with primers directed against this gene.
dsh is necessary for the dorsal-ventral polarization of leading edge cells early in dorsal closure, in the absence of which actin-cable assembly and actin-based cell process formation fails to occur properly in these cells.
fz-mediated signalling provides polarity information to pI, specifying the orientation of mitotic spindles. The orientations of the pIIa and pIIb divisions are independent of fz signalling and are instead defined by the axis of the previous pI division.
dsh interacts antagonistically with N and Dl. A physical interaction of the dsh product with the carboxy terminus of that of N suggests a basis for the interaction. Thus dsh, in addition to transducing wg signal, blocks N signalling directly, explaining the inhibitory cross talk observed between the pathways.
dsh mutations mimic all effects of a wg mutation. sgg, dsh and arm function to transmit the wg signal in the midgut in the same way as they do in the epidermis. Results suggest the wg signal transduction pathway acts in all three germ layers, the ectoderm, mesoderm and endoderm.
wg is expressed in a narrow stripe at the wing margin, dsh is required in the cells responding to wg. Clones of dsh mutant tissue at the wing margin can block the response to wg. The wg signal can traverse several cell diameters of mutant tissue to reach responsive wing cells. Overexpression of dsh potentiates the response to wg, bristle formation is induced large distances from the site of wg expression. wg can signal over a large distance in a sensitized background.
Phenotypic and mosaic analysis of dsh reveals functions in cellular responses to multiple positional signalling mechanisms in epidermal development.
Most alleles lethal, but dsh1, although poorly viable as homozygous females or hemizygous males, appears to be fully viable when heterozygous to a deficiency for the region, suggesting that the reduced viability is unrelated to dsh1. dsh1 flies have deranged thoracic hairs, divergent and blistered wings and ellipsoid eyes. Leg bristles, hairs and bracts display high frequencies of abnormal polarity; extra joints or joint primordia found frequently in the first and second tarsal joints of the first and second pairs of legs; of 270 ectopic joints, 268 displayed inverted polarity [Held, Duarte and Derakhshanian, 1986). Males and females fertile, males weakly so. Homozygotes and hemizygotes for the lethal alleles die as second- to early-third instar larvae when derived from heterozygous mothers; when derived from homozygous germ-line clones, on the other hand, embryos with segment-polarity defects result; only ventral cuticle is present, covered with a lawn of setae; lack dorsal cuticle, posterior spiracles and filzkorper material. At six to seven hours maxillary and labial segments appear to be missing and parasegmental boundaries do not form; cell death apparent in vicinity of tracheal pits, which subsequently fuse; segmental boundaries fail to form; organization of central nervous system seems normal. Loss of cells of posterior segment compartments leads to discontinued production of en+ product. Viability of dsh1/dsh3, for example, when derived from homozygous dsh3 oogenic clones, is normal, indicating that dsh1 is wild type for the early function.