E86
homeodomain transcription factor - confers neural identity in specific neurons of medulla and lamina of the optic lobe
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.52
2.0 (northern blot)
226 (aa)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\bsh using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: reference states >6 hr AEL
The rare bsh transcripts appear at 6hr of embryogenesis and are observed trhoughout development. Highest levels occur between 6 and 24 hours of embryogenesis. In third instar larvae, expression is restricted to the developing optic lobes in cells that have just arisen from the inner and outer proliferation centers. Low levels of staining are observed in adults over the cell body layer of the lamina.
At third larval instar, bsh protein is expressed in the medial most neurons in the developing lamina, overlapping with dac. Some of these neurons will develop into lamina monopolar L5 neurons. At 12h APF, bsh is expressed in 2-4 rows of cells, and at 24h APF, its expression is restricted to the two medial rows of cells, overlapping with EcollacZap-rK568 in the most lateral row. In adults, bsh is expressed in the lamina monopolar L4 and L5 neurons.
bsh protein is expressed in the differentiated neurons of the inner domain of the medulla anlage from third instar larvae and in pupa until 12h APF. It co-localises with hth in the whole of its domain. Thereafter and in adults, bsh protein is found in layers 8 to 10 and in the cortex of the medulla.
bsh protein is firstdetected in embryos in 4 widely separated cells, two in each procephaliclobe, at stage 11. These cells are likely to derive from the neurogenicectoderm. In subsequent stages, the number of bsh-expressing cellsincreases in two bilateral clusters adjacent to the original two cells. Asthe brain continues to develop, the number of bsh-expressing cellscontinues to increase. By stage 16, approximately 30 bsh-expressingcells can be counted in each brain hemisphere. These cells are clusteredin four separate regions of the brain. At stage 16 one of thebsh-expressing cells is closely associated with the terminus to Bolwig'snerve.
Comment: 24h to 72h APF
Comment: 24h to 72h APF
JBrowse - Visual display of RNA-Seq signals
View Dmel\bsh in JBrowse2-54
2-53.7
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
monoclonal
polyclonal
bsh is required and largely sufficient for medulla intrinsic Mi1 neuron specification in the medulla and L4 neuron specification in the lamina. Additionally, it is at least required for L5 neuron specification in the lamina.
The bsh gene may play a highly specialized role in the determination and function of cell type in the brain. The smallest deletion interval that eliminates bsh (the overlap of Df(2L)OD16 and Df(2L)pr65, which may also delete other genes) produces a first instar larval lethal phenotype, and the larval brains are not grossly abnormal.
Identified on the basis of similarity to the eve homeobox sequence.
Source for merge of: bsh Hox11-310
