NURF301, NURF, p301, dNURF, Nurf-215
major subunit of nucleosome remodeling factor (nurf) - multiple domain protein with HMGI/Y and bromodomain motifs - interacts physically and functionally with the TRF2/DREF basal transcription factor to organize nucleosomes downstream of active promoters
Please see the JBrowse view of Dmel\E(bx) for information on other features
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Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Low-frequency RNA-Seq exon junction(s) not annotated.
Apparent introns not annotated: probable artifacts due to repetitive sequence.
Gene model reviewed during 5.45
Component of the NURF complex composed of Caf1-55, E(bx), Nurf-38 and Iswi. Interacts with Trl. Interacts with histone H3-K4Me3.
The second PHD-type zinc finger mediates binding to histone H3K4Me3.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\E(bx) using the Feature Mapper tool.
E(bx) is expressed in all cells in the testis apex including the hub cells, germline stem cells, and cyst progenitor cells.
GBrowse - Visual display of RNA-Seq signals
View Dmel\E(bx) in GBrowse 23-1
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: E(bx) Nurf301
Source for merge of: E(bx) l(3)ry122
Source for merge of: E(bx) CG10894
Source for merge of: Nurf301 CG17135 CG7022
Annotations CG10894, CG17135 and CG7022 merged as CG32346 in release 3 of the genome annotation.
"p230", "p250" and "p300" do not correspond to "E(bx)".
Source for merge of Nurf301 CG17135 CG7022 was sequence comparison ( date:011022 ).
dsRNA has been made from templates generated with primers directed against this gene. RNAi of E(bx) results in increased arborization of ddaD and ddaE neurons, defects in dendrite morphogenesis and reproducible defects in da dendrite development.
E(bx) is required for induction of the heat shock genes and has additional functions during larval blood development.
E(bx) is a component of the NURF complex that is both necessary and sufficient for accurate and efficient nucleosome sliding. It interacts with sequence-specific transcription factors, providing a basis for targeted recruitment of the NURF complex to specific genes.
NURF, a protein complex of four subunits Caf1, Nurf-38, E(bx) and Iswi, is able to facilitate transcription mediated by a Scer\GAL4 derivative carrying a constitutively activating region from Hsf. Once nucleosome remodelling by the DNA binding factor is accomplished a high level of NURF activity is not continuously required for recruitment of the general transcription machinery and transcription initiation. NURF is able to assist gene activation in a chromatin context: remodel nucleosomes for gene activation in chromatin.
Nucleosome remodelling factor is composed of at least four polypeptides that act in concert with the Trl transcription factor at the Hsp70 promoter. Nucleosome remodelling factor acts directly on the nucleosome to perturb its structure.
E. B. Lewis.
