Gene model reviewed during 5.45
Gene model reviewed during 5.54
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Edg78E using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Edg78E in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.
Temporal profile of gene expression is altered in Eip74EF mutant background.
Initial characterization of Edg78E suggests the gene encodes a cuticle protein.
The spatial and temporal expression is as expected for genes that encode pupal cuticle proteins.
mRNA on Northerns: 0.6 kb Identification: Differential screen of a genomic library with cDNA isolated from imaginal discs pulsed with 20-hydroxyecdysone, 20-HE (Fechtel, Natzle, Brown and Fristrom, 1988). Transcripts in imaginal disc tissue, beginning at six to nine hours, following a six-hour 20-HE pulse, but not in the absence or continual presence of 20-HE; mRNA's enriched in membrane-bound polysome fractions of imaginal discs. Translation product from hybrid selected mRNA of Edg78E reacts with polyclonal antibodies prepared against purified pupal cuticle proteins.