l(2)02029, VASP, enb
VASP homolog and SH3 domain protein - acts as processive actin polymerase, stimulating actin addition at the barbed end - cytoskeletal adaptor protein acting in epithelial morphogenesis - implicated in axonal outgrowth and fasciculation
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.50
3.4 (longest cDNA)
684 (aa); 80 (kD observed); 72 (kD predicted)
Interacts with Abl and Src SH3 domains. Binds, in vitro and in vivo, the cytoplasmic domain of robo. Interacts with Zyx102EF and chic.
Tyrosine phosphorylated on multiple sites by Abl kinase. In vitro, phosphorylation on specific tyrosine residues inhibits interaction with Abl and Src SH3 domains.
The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ena using the Feature Mapper tool.
ena protein is expressed in somatic gonadal precursor cells from embryonic stage 12 though gonad coalescence.
ena protein localizes to adherens junctions and the cytoplasm, from after cellularization, through gastrulation and into the extended germband stage. After germband extension, ena protein remains at adherens junctions and concentrates at epidermal tricellular junctions; it is uniformly localized around the amnioserosal cell cortex. During dorsal closure, ena protein becomes concentrated along the front of leading-edge cells, becoming strongly enriched in spots at leading edge adherens junctions, but remaining at tricellular junctions. ena protein is especially enriched in the single row of cells per segment that initiate segmental grooves and in cells at the anterior edge of the dorsal fold that lead during head involution.
During the initialstages of dorsal closure, as leading edge cells begin to elongate, ena protein surrounds the cell membrane but is enriched at sites of cell-cell contact and at adherens junctions of leading edge cells. As closure proceeds, ena protein accumulates at uniformly high levels at the adherens junctions of leading edge cells. ena protein localizes to the cytoplasm in a stripe of epidermal cells at the segmental boundary. ena protein is also observed in the embryonic hindgut, in axons of the embryonic CNS, in adherens junctions of imaginal disc epithelial cells, and in the specialized junctions of the photoreceptor rhabdomeres.
ena protein is first detected in embryonic stage 10 and by stage 11 is found in the endoderm and ectoderm of the extended germ band. During germ band retraction, expression becomes restricted to the CNS and by stage 15 is found primarily in the commissural and longitudinal axons of the CNS. During late embryogenesis, ena is localized primarily to the longitudinal axons.
GBrowse - Visual display of RNA-Seq signalsView Dmel\ena in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: reduced F-actin and altered cell shape. Kc167 cells show change from round to spindle-shaped, with the formation of F-actin puncta and microtubule extensions.
dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.
Mutant phenotype indicates a role for the ena protein in the organisation of the embryonic PNS and CNS. A critical function of Abl is to phosphorylate and negatively regulate ena protein during neural development.
Alleles act as suppressors of Abl mutant phenotype.