Open Close
General Information
Symbol
Dmel\fzy
Species
D. melanogaster
Name
fizzy
Annotation Symbol
CG4274
Feature Type
FlyBase ID
FBgn0001086
Gene Model Status
Stock Availability
Gene Summary
fizzy (fzy) encodes WD40 domain protein required for the full ubiquitin ligase activity of the anaphase-promoting complex/cyclosome (APC/C) in mitosis and meiosis. The product of fzy functions to target substrates for destruction and drive metaphase and anaphase transition. [Date last reviewed: 2018-09-13] (FlyBase Gene Snapshot)
Also Known As

Cdc20, BG:DS02740.14

Key Links
Genomic Location
Cytogenetic map
Sequence location
2L:16,310,956..16,313,097 [+]
Recombination map
2-52
RefSeq locus
NT_033779 REGION:16310956..16313097
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (16 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000460086
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000460086
(assigned by GO_Central )
Biological Process (10 terms)
Terms Based on Experimental Evidence (8 terms)
CV Term
Evidence
References
involved_in female meiosis II
inferred from mutant phenotype
involved_in female meiosis I
inferred from genetic interaction with FLYBASE:cort; FB:FBgn0000351
inferred from mutant phenotype
involved_in mitotic cell cycle
inferred from high throughput mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from sequence or structural similarity with HGNC:24824
inferred from biological aspect of ancestor with PANTHER:PTN000460086
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000460086
(assigned by GO_Central )
inferred from sequence or structural similarity with HGNC:24824
Cellular Component (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
inferred from direct assay
located_in centrosome
inferred from direct assay
located_in kinetochore
inferred from direct assay
located_in spindle
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from sequence or structural similarity with HGNC:24824
inferred from biological aspect of ancestor with PANTHER:PTN000460086
(assigned by GO_Central )
Gene Group (FlyBase)
Protein Family (UniProt)
-
Summaries
Gene Snapshot
fizzy (fzy) encodes WD40 domain protein required for the full ubiquitin ligase activity of the anaphase-promoting complex/cyclosome (APC/C) in mitosis and meiosis. The product of fzy functions to target substrates for destruction and drive metaphase and anaphase transition. [Date last reviewed: 2018-09-13]
Gene Group (FlyBase)
ANAPHASE-PROMOTING COMPLEX -
Anaphase-promoting complex/cyclosome (APC/C) is a multisubunit E3 ubiquitin ligase. Active during mitosis and G1 phases of the cell cycle, it mediates the degradation of cyclins and other key cell cycle regulators triggering the metaphase to anaphase transition and the exit from mitosis. (Adapted from PMID:12208841).
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
fzy: fizzy
Homozygous lethal; ventral outside and central nervous system degenerate.
Summary (Interactive Fly)

WD40 domain protein - homolog of Cdc20 - required for the activity of the anaphase promoting complex in mitosis - functions to target the three mitotic cyclins A, B and B3 for destruction in the egg and drive anaphase progression during meiosis

Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\fzy for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.48

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0080833
1996
526
Additional Transcript Data and Comments
Reported size (kB)

1.9 (longest cDNA)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0080391
57.1
526
8.98
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

526 (aa); 59 (kD observed)

Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\fzy using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

antennal anlage

Comment: reported as procephalic ectoderm anlage

central brain anlage

Comment: reported as procephalic ectoderm anlage

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

visual anlage

Comment: reported as procephalic ectoderm anlage

antennal primordium

Comment: reported as procephalic ectoderm primordium

central brain primordium

Comment: reported as procephalic ectoderm primordium

visual primordium

Comment: reported as procephalic ectoderm primordium

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

fzy protein is ubiquitous in stage 5 to 10 embryos, but its expression is restricted to proliferating cells after stage 11. The highest level of fzy protein in stage 11 embryos is found in the CNS precursors. At stage 14, the neuroblasts and ganglion mother cells at the edges of the ventral nerve cord and the brain lobes contain fzy protein.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
located_in centrosome
inferred from direct assay
located_in kinetochore
inferred from direct assay
located_in spindle
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\fzy in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 13 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 18 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of fzy
Transgenic constructs containing regulatory region of fzy
Aberrations (Deficiencies and Duplications) ( 64 )
Inferred from experimentation ( 64 )
Gene not disrupted in
Inferred from location ( 0 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
mitosis & nuclear chromosome
mitotic cell cycle & epidermis & embryo | ventral
mitotic cell cycle & spindle & epidermis & embryo | ventral
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
6 of 15
No
Yes
4 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
 
2  
6 of 15
No
Yes
4 of 15
No
No
Rattus norvegicus (Norway rat) (3)
13 of 13
Yes
Yes
4 of 13
No
No
2 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (4)
9 of 12
Yes
Yes
3 of 12
No
No
2 of 12
No
Yes
1 of 12
No
Yes
Danio rerio (Zebrafish) (3)
12 of 15
Yes
Yes
4 of 15
No
No
4 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (2)
6 of 15
Yes
Yes
4 of 15
No
No
Arabidopsis thaliana (thale-cress) (9)
8 of 9
Yes
Yes
8 of 9
Yes
Yes
7 of 9
No
Yes
7 of 9
No
Yes
7 of 9
No
Yes
5 of 9
No
Yes
2 of 9
No
No
2 of 9
No
No
2 of 9
No
No
Saccharomyces cerevisiae (Brewer's yeast) (3)
9 of 15
Yes
Yes
3 of 15
No
No
3 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (5)
9 of 12
Yes
Yes
3 of 12
No
Yes
2 of 12
No
No
2 of 12
No
Yes
2 of 12
No
No
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091906OF )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915059V )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W05HG )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Danaus plexippus
Monarch butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X05DJ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G06FJ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (3)
6 of 10
6 of 10
2 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Dmel gene
    Ortholog showing functional complementation
    Supporting References
    Interactions
    Summary of Physical Interactions
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map
    2-52
    Cytogenetic map
    Sequence location
    2L:16,310,956..16,313,097 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    35F1-35F1
    Limits computationally determined from genome sequence between P{lacW}heixk11403&P{lacW}heixk12401 and P{EP}fzyEP1028&P{lacW}mRpL4k14608
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    35F8-35F11
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (17)
    Genomic Clones (14)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (134)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of

    Source for identity of: fzy CG4274

    Source for database merge of
    Additional comments
    Other Comments

    Eggs derived from mutant females almost invariably arrest in anaphase of meiosis II.

    cort and fzy are required for anaphase progression in both female meiotic divisions, performing partially redundant roles in meiosis I and non-redundant roles in meiosis II.

    SL2 cells treated with dsRNA against fzy show a clear increase in the mitotic index compared to control cells.

    S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

    RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.

    RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.

    dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

    RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 and S2R+ cells: cell morphology is aberrant and there is an increased frequency of microtubule-based mitotic spindles, indicative of a failure in mitosis.

    fzy is required for timely disengagement of mother and daughter centrioles in embryos.

    The metaphase arrest seen in fzy mutants persists in the absence of pim or thr function.

    fzy has been cloned and sequenced, and its expression pattern has been analysed.

    Mutations in fzy result in metaphase arrest, neither CycA, CycB or CycB3 are degraded in this arrest. Comparison of the fzy mutant phenotype with the phenotype resulting from expression of N-terminal truncated CycA, CycB or CycB3 suggests that fzy is not only required for mitotic cyclin degradation because fzy mutations, but not truncated cyclins, block chromosome separation. Inactivation of a temperature sensitive cdc2 in fzy metaphase arrest causes a reversion to interphase morphology. This reversion is not accompanied by completion of mitosis so there is no increase in anaphase cells or change in cell number or size.

    The fizzy gene function is required maternally and zygotically for dividing cells to exit metaphase and complete mitosis. Zygotic fzy is required for normal differentiation of the ventral neurogenic ectoderm. Double mutant analysis shows that stg is epistatic to fzy.

    3 additional alleles are discussed but are not named.

    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 80 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DPiM - Drosophila Protein interaction map
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (21)
    Reported As
    Symbol Synonym
    Cdc20fizzy
    cdc20/fizzy
    Secondary FlyBase IDs
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (191)