gsb-p, gsb, gsbn, BSH4, gsb-neuro
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.52
There is only one protein coding transcript and one polypeptide associated with this gene
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\gsb-n using the Feature Mapper tool.
gsb-n protein is first detected at embryonic stage 10, in a subset of neuroblasts, ganglion mother cells, and neurons. By the end of stage 11 neuronal expression has intensified and is segmentally repeated, from the mandibular to the abdominal neuromere. Expression is also detected in the terminal regions, like the neurons of the brain in the head, the neurons of abdominal segment 9, and the cells of the anal pad in the tail region. Subsequently expression continues to be detected in a subset of neurons until stage 17. Additional expression is detected after germ band retraction in a few lateral cells per hemisegment, in ventral ectodermal stripes in the posterior region of each segment, in patches of epidermal cells or their derivatives in the head region (such as the pharynx), and in the nuclei of one of the ventral superficial oblique muscles. Expression of en and gsb-n proteins overlap extensively in the central nervous system.
GBrowse - Visual display of RNA-Seq signalsView Dmel\gsb-n in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: gsb-n CG2692
gsb-n null mutants are sub-viable and all surviving males and most females are sterile.
Both gsb-n and gsb are involved in specification of the SNa motor neurons, but gsb appears to play the more important role (since while two doses of gsb or gsb-n are required to properly determine SNa fate, one of the doses must be gsb, whereas gsb-n is dispensible when two copies of gsb are present). gsb and gsb-n have partially redundant functions in embryonic segmentation.
Segment polarity gene expression is necessary for the survival of specific rows of epidermal cells.
The expression pattern of a number of genes in the larval genital discs, including gsb-n, has been studied to determine the segment-parasegment organisation of the genital discs.
gsb-n is expressed in a lineage-specific fashion which is consistent with the action of a selector gene.
gsb and gsb-n both share the same upstream sequence which includes the enhancer elements for gsb and gsb-n. Activation of gsb-n depends on gsb, both of which are probably expressed in the same neuroblasts.
Ectopic expression analysis demonstrates that the gsb-n gene product can substitute the function of the prd gene product during early embryonic development and prd gene product can substitute gsb-n gene product during late embryonic development in regulating expression of wg and en.
Comparisons of early development to that in other insects have revealed conservation of some aspects of development, as well as differences that may explain variations in early patterning events.
The establishment and maintenance of gsb stripes is under the control of two separable and consecutively acting cis-regulatory elements, the gsb-early element, or GEE, and the gsb-late element, or GLE. The GEE is activated by pair-rule proteins, to establish the gsb stripes while the GLE controls their maintenance in response to the wg signal rather than to the gsb protein itself.
gsb, gsb-n mutants are homozygous lethal; embryos show segment-polarity defects. The posterior portion of each segment is deleted and the anterior portion duplicated in mirror image fashion; the ventral segments are almost entirely covered with denticles, the posterior fraction of which point anteriorly. Segment boundaries persist normally and segments maintain their individuality.
gsb-n and gsb have a specific role in the control of cell fates during neurogenesis. Mutants display a complex series of alterations in neuronal identity, both underneath and outside the modified ectoderm; posterior commissures are almost totally absent.
Included in genetic and molecular analysis of the zipper-gooseberry region: no point mutations in gsb were obtained.