Gene model reviewed during 5.44
Gene model reviewed during 5.46
Gene model reviewed during 5.55
There is only one protein coding transcript and one polypeptide associated with this gene
466 (aa); 75 (kD observed); 48 (kD predicted)
The regions 203-253, 256-333 and 335-377 are thought to contain either alpha helical or beta pleated sheet motifs.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ImpE2 using the Feature Mapper tool.
Northern blotting reveals that the highest levels of ImpE2 transcript are present in white prepupae and early (24hr) pupae. Moderate levels are seen in 12-18 hr embryos and in late pupae (>48 hr). Low levels of transcript are found in 3-12 hour embryos and in larvae, while no transcripts are detected in adults, or in 0-3 hour embryos. When third instar larval imaginal discs are cultured for 3, 6, 9 or 12 hr in the presence of 20-hydroxyecdysone (20-HE), the 1.7 kb ImpE2 transcript is detected, while no transcript is detected in discs cultured for the same length of time without 20-HE. Radioactive in situ hybridization shows that in 12-15 hour embryos, transcript localizes to the ectoderm. In 0-4 hour prepupae, part of the columnar epithelium and the peripodal epithelium of each imaginal disc, and the epithelium of the proventriculus, accumulates ImpE2 transcript.
GBrowse - Visual display of RNA-Seq signalsView Dmel\ImpE2 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Expression throughout the onset of metamorphosis is not affected by Eip74EF mutations.
Gene encoding a transcript associated with membrane-bound polysomes in imaginal discs and expressed only in response to 20-hydroxyecdysone (20 HOE). Expression studied both in vitro and in vivo. One of the "early" genes involved in elongation of appendage-forming regions of the imaginal discs by means of epithelial-cell rearrangements (Natzle, Fristrom and Fristrom, 1988).