FlyBase Gene Report: Dmel\ImpE2

General Information

Symbol

Dmel\ImpE2

Species

D. melanogaster

Name

Ecdysone-inducible gene E2

Annotation Symbol

CG1934

Feature Type

protein_coding_gene

FlyBase ID

FBgn0001254

Gene Model Status

Current

Stock Availability

5 publicly available

Gene Summary

Probably has an essential role in embryogenesis, induces morphogenesis of imaginal disks, and may participate in multimolecular aggregates. (UniProt, P29681)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
UniProtKB
Red Book
Interactive Fly

Also Known As

IMP-E2

Key Links

Genomic Location

Cytogenetic map

63E2-63E2

Sequence location

3L:3,510,974..3,512,681 [-]

Recombination map

3-8

RefSeq locus

NT_037436 REGION:3510974..3512681

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (4 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in imaginal disc eversion

traceable author statement

(Andres et al., 1993)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Paine-Saunders et al., 1990)

located_in extracellular region

inferred from direct assay

(Paine-Saunders et al., 1990)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in extrinsic component of membrane

traceable author statement

(Andres et al., 1993)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Protein Function (UniProtKB)

Probably has an essential role in embryogenesis, induces morphogenesis of imaginal disks, and may participate in multimolecular aggregates.

(UniProt, P29681)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Imp-E: Inducible membrane-bound polysomes-Early

Name given to three genes encoding transcripts associated with membrane-bound polysomes in imaginal discs and expressed only in response to 20-hydroxyecdysone (20 HOE). Expression studied both in vitro and in vivo. Imp-E denotes three "early" genes involved in elongation of appendage-forming regions of the imaginal discs by means of epithelial-cell rearrangements (Natzle et al., 1988).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\ImpE2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P29681)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.44

Gene model reviewed during 5.46

Gene model reviewed during 5.55

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

ImpE2-RA

FBtr0073141

NM_057232

1708

466

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

ImpE2-PA

FBpp0073000

49.8

466

4.06

P29681

NP_476580

AAF47780

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

466 (aa); 75 (kD observed); 48 (kD predicted)

(Paine-Saunders et al., 1990)

Comments

External Data

Domain

The regions 203-253, 256-333 and 335-377 are thought to contain either alpha helical or beta pleated sheet motifs.

(UniProt, P29681)

Crossreferences

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ImpE2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ImpE2_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.36

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 6

anterior endoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

endoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

foregut anlage in statu nascendi

(Fisher et al., 2012)

posterior ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 7 -- 8

antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

anterior endoderm anlage

(Fisher et al., 2012)

foregut anlage

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic dorsal epidermis

(Fisher et al., 2012)

embryonic esophagus

(Fisher et al., 2012)

embryonic foregut

(Fisher et al., 2012)

embryonic head sensory system

(Fisher et al., 2012)

embryonic salivary gland duct

(Fisher et al., 2012)

embryonic stomatogastric nervous system

(Fisher et al., 2012)

embryonic ventral epidermis

(Fisher et al., 2012)

embryonic/larval corpus cardiacum

(Fisher et al., 2012)

embryonic stage 15 -- 16

ectoderm

Comment: reference states 12-15 hr AEL

(Paine-Saunders et al., 1990)

prepupal stage P1 -- P3

embryonic/larval proventriculus

Comment: reference states 0-4 hr APF

(Paine-Saunders et al., 1990)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- pupal stage

(Paine-Saunders et al., 1990)

Additional Descriptive Data

Northern blotting reveals that the highest levels of ImpE2 transcript are present in white prepupae and early (24hr) pupae. Moderate levels are seen in 12-18 hr embryos and in late pupae (>48 hr). Low levels of transcript are found in 3-12 hour embryos and in larvae, while no transcripts are detected in adults, or in 0-3 hour embryos. When third instar larval imaginal discs are cultured for 3, 6, 9 or 12 hr in the presence of 20-hydroxyecdysone (20-HE), the 1.7 kb ImpE2 transcript is detected, while no transcript is detected in discs cultured for the same length of time without 20-HE. Radioactive in situ hybridization shows that in 12-15 hour embryos, transcript localizes to the ectoderm. In 0-4 hour prepupae, part of the columnar epithelium and the peripodal epithelium of each imaginal disc, and the epithelium of the proventriculus, accumulates ImpE2 transcript.

(Paine-Saunders et al., 1990)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 15 -- 16

ectoderm

Comment: reference states 12-15 hr AEL

(Paine-Saunders et al., 1990)

Additional Descriptive Data

The ImpE2 protein is detected in the ectoderm, foregut and hindgut of 12-15 hr embryos. In 0-4 hr prepupae, the imaginal disc epithelium and the apical surface of the proventriculus are stained.

(Paine-Saunders et al., 1990)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Paine-Saunders et al., 1990)

located_in extracellular region

inferred from direct assay

(Paine-Saunders et al., 1990)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\ImpE2 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\ImpE2

View exonic expression by tissue for Dmel\ImpE2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0001254

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38432/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001254

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001254

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001254

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1934

Flygut - An atlas of the Drosophila adult midgut

FBgn0001254

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\ImpE2

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of ImpE2

Transgenic constructs containing regulatory region of ImpE2

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(3L)Exel6095

(Ryder, 2004.4.26)

Df(3L)HR119

(Paine-Saunders et al., 1990)

Inferred from location ( 2 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

ImpE2^GD5839

viable, with Scer\GAL4^Mef2.PR

ImpE2^GD5839

viable, with Scer\GAL4^pnr-MD237

ImpE2^GD5839

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (0)

Arabidopsis thaliana (thale-cress) (1)

Atha\AT1G67120

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:ImpE2. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

63925

DroID - A comprehensive database of gene and protein interactions.

FBgn0001254

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-8

Cytogenetic map

63E2-63E2

Sequence location

3L:3,510,974..3,512,681 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

63E2-63E2

Limits computationally determined from genome sequence between P{PZ}kst⁰¹³¹⁸ and P{PZ}Sc2⁰⁵⁶³⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

63E-63E

(determined by in situ hybridisation)

(Paine-Saunders et al., 1990, Natzle et al., 1986)

Experimentally Determined Recombination Data

Location

3-8

(FlyBase, 2016)

3-7.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (5)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

82502

y¹ sc^* v¹ sev²¹; P{WKO.3-A4}attP40

35235

y¹ sc^* v¹ sev²¹; P{TRiP.GL00120}attP2

55380

y¹ sc^* v¹ sev²¹; P{TRiP.HMC04068}attP40

VDRC

v100148

P{KK105038}VIE-260B

v16842

w¹¹¹⁸; P{GD5839}v16842/CyO

Genomic Clones (34)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (60)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD19609

Other clones

DPiM_LD19609

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0001254

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

LP02336
LP04186
LP04921
LP04943
LP05279
LP06952
LP07016
LP07355
LP07985
LP08783
LP10170
LP10416
LP15427
LP15871
LP15993
LP16918
LP18145
LP19163
LP19519
LP19862
LP19989
LP20137
LP20776
LP20856
LP22066
LP24011
RE09796
RE44285
RE50912
RE52312

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0001254

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Expression throughout the onset of metamorphosis is not affected by Eip74EF mutations.

(Fletcher and Thummel, 1995)

Ecdysteroid-regulated gene.

(Andres et al., 1993)

ImpE2 has been sequenced and its expression pattern analysed.

(Paine-Saunders et al., 1990)

Gene encoding a transcript associated with membrane-bound polysomes in imaginal discs and expressed only in response to 20-hydroxyecdysone (20 HOE). Expression studied both in vitro and in vivo. One of the "early" genes involved in elongation of appendage-forming regions of the imaginal discs by means of epithelial-cell rearrangements (Natzle, Fristrom and Fristrom, 1988).

Relationship to Other Genes

Source for database merge of

Additional comments

"ImpE2" may correspond to the "l(3)63Dc" locus.

(Munroe et al., 1998.5.12)

Nomenclature History

Source for database identify of

Source for identity of: ImpE2 CG1934

(Kodira, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CG1934

(Heinrichsen et al., 2014, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Carrera et al., 2008, Haussmann et al., 2008)

IMP-E2

(Beckstead et al., 2005, Fristrom and Fristrom, 1993, Andres and Thummel, 1992, Paine-Saunders, 1991.1.30, Paine-Saunders et al., 1990, Thummel, 1990)

Imp-E2

(Bayer et al., 1996, Fletcher and Thummel, 1995, Andres et al., 1993)

Name Synonyms

Ecdysone-inducible gene E2

(Gim et al., 2001)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 30 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38432

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P29681

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P29681

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P29681

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0001254

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38432/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001254

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001254

FlyMine - An integrated database for Drosophila genomics

FBgn0001254

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG1934

MARRVEL_MODEL - MARRVEL (model organism gene)

38432

Linkouts

BioGRID - A database of protein and genetic interactions.

63925

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0001254

DroID - A comprehensive database of gene and protein interactions.

FBgn0001254

DRSC - Results frm RNAi screens

FBgn0001254

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ImpE2_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001254

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0001254

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1934

Flygut - An atlas of the Drosophila adult midgut

FBgn0001254

References (70)

The following external sites may use different assemblies or annotations than FlyBase.

-0.36

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