Gene model reviewed during 5.47
There is only one protein coding transcript and one polypeptide associated with this gene
140 (aa); 15 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\janB using the Feature Mapper tool.
janB transcript is first detected in spermatocytes, and signal increases during spermatocyte growth. Signal decreases sharply after meiosis, and cannot be detected in sperm bundles.
The reporter protein is detected in the central region of the testes, which contains later stages of spermatogenesis. Reporter protein begins to be detected in the late pupa, and is not detected in larvae or early pupae.
GBrowse - Visual display of RNA-Seq signalsView Dmel\janB in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
The molecular organization of the 'rp49-Sry-jan' gene cluster, including gene order, direction of transcription and partial overlap of the janA and janB genes is strictly conserved between D.melanogaster and D.pseudoobscura.pseudoobscura.
Analysis of the expression of a number of janB-Ecol\lacZ constructs shows that the testis-specific expression of janB is mediated by a short DNA sequence (from -174 to +107bp) that is located entirely within the last exon of the upstream, partially overlapping, janA gene. The presence in cis of an active janA gene leads to a reduced accumulation of janB-Ecol\lacZ hybrid RNA, supporting the hypothesis that janA transcription interferes with the function of the janB cis-regulatory elements.
One of two partially overlapping genes, janA and janB, regulate the expression of somatic sex differentiation. janA is expressed in a complex way in both males and females; janB is only expressed in males (Yanicostas, Vincent and Lepesant, 1989).