FlyBase Gene Report: Dmel\egh

FB2026_02 , released June 18, 2026

FB2026_02 , released June 18, 2026

Gene: Dmel\egh

General Information

Symbol

Dmel\egh

Species

D. melanogaster

Name

egghead

Annotation Symbol

CG9659

Feature Type

protein_coding_gene

FlyBase ID

FBgn0001404

Gene Model Status

Stock Availability

18 publicly available

Gene Summary

egghead (egh) encodes a Golgi β1,4-mannosyltransferase that catalyzes the transfer of a mannose residue in a β1,4-linkage to the glucose residue of glucosylceramide (GlcCer). It is involved in the biosynthesis of the glycan core of glycosphingolipids. [Date last reviewed: 2025-08-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

zw4, l(1)zw4, zw-4, EG:BACR25B3.8 , zeste-white 4

Key Links

Genomic Location

Cytogenetic map

Sequence location

X:2,588,503..2,599,111 [+]

Recombination map

1-1

RefSeq locus

NC_004354 REGION:2588503..2599111

Sequence

Get Decorated FASTA

Genomic Maps

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (21 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables beta-1,4-mannosyltransferase activity

inferred from direct assay

(Wandall et al., 2003)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables beta-1,4-mannosyltransferase activity

inferred from biological aspect of ancestor with PANTHER:PTN001047798

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR027389

(InterPro Project Members, 2004-)

Biological Process (17 terms)

Terms Based on Experimental Evidence (17 terms)

CV Term

Evidence

References

involved_in arthro-series glucosylceramide biosynthetic process

inferred from direct assay

(Wandall et al., 2005, Wandall et al., 2003)

involved_in axon guidance

inferred from mutant phenotype

(Soller et al., 2006)

involved_in border follicle cell migration

inferred from mutant phenotype

(Goode et al., 1996)

involved_in cell fate commitment

inferred from mutant phenotype

(Goode et al., 1996)

inferred from mutant phenotype

(Rubsam et al., 1998)

involved_in follicle cell of egg chamber development

inferred from mutant phenotype

(Rubsam et al., 1998, Goode et al., 1996)

involved_in follicle cell of egg chamber-cell adhesion

inferred from mutant phenotype

(Goode et al., 1996)

involved_in germ cell development

inferred from mutant phenotype

(Rubsam et al., 1998)

involved_in germarium-derived egg chamber formation

inferred from mutant phenotype

(Goode et al., 1996)

involved_in insect visual primordium formation

inferred from mutant phenotype

(Fan et al., 2005)

involved_in maintenance of polarity of follicular epithelium

inferred from mutant phenotype

(Goode et al., 1996)

involved_in male courtship behavior

inferred from mutant phenotype

(Ellis and Carney, 2011)

involved_in morphogenesis of follicular epithelium

inferred from mutant phenotype

(Goode et al., 1996)

involved_in negative regulation of female receptivity, post-mating

inferred from mutant phenotype

(Soller et al., 2006)

involved_in oocyte localization involved in germarium-derived egg chamber formation

inferred from mutant phenotype

(Rubsam et al., 1998)

involved_in oocyte microtubule cytoskeleton organization

inferred from mutant phenotype

(Rubsam et al., 1998)

involved_in peripheral nervous system neuron development

inferred from mutant phenotype

(Dahlgaard et al., 2012)

involved_in regulation of egg-laying behavior

inferred from mutant phenotype

(Soller et al., 2006)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN001047798

(GO Reference Genome Project, 2011-)

located_in Golgi membrane

traceable author statement

(Wandall et al., 2005)

inferred from experiment

(Wandall et al., 2003)

located_in membrane

non-traceable author statement

(Goode et al., 1996)

Gene Group (FlyBase)

UNCLASSIFIED MANNOSYLTRANSFERASES

Pathway (FlyBase)

GLUCOSYLCERAMIDE BIOSYNTHESIS

Protein Family (UniProt)

Belongs to the glycosyltransferase 2 family. (O01346)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

egghead (egh) encodes a Golgi β1,4-mannosyltransferase that catalyzes the transfer of a mannose residue in a β1,4-linkage to the glucose residue of glucosylceramide (GlcCer). It is involved in the biosynthesis of the glycan core of glycosphingolipids. [Date last reviewed: 2025-08-07]

Gene Group (FlyBase)

UNCLASSIFIED MANNOSYLTRANSFERASES -: This group comprises mannosyltransferases that do not classify under other groups in FlyBase.

Pathway (FlyBase)

GLUCOSYLCERAMIDE BIOSYNTHESIS -: The biosynthesis of glucosylceramides, a type of glycosylsphingolipids, occurs in the Golgi apparatus and involves the sequential attachment of sugars to a ceramide lipid moiety that is exported from the endoplasmic reticulum. In D. melanogaster, the core of glucosylceramides is composed of a disaccharide core of mannose(β1,4)glucose β-linked to a ceramide, known as mactosylceramide (MacCer). The MacCer core is extended by the sequential addition of monosaccharides to form the 'arthro-series' of neutral, acidic, and zwitterionic glucosylceramides with variable complexity and oligosaccharide length. The N-acetylglucosamine residues can be further modified with phosphoethanolamine (pEtn). Glucosylceramides are usually transported to the plasma membrane and are often enriched in membrane microdomains, known as lipid rafts. They play various roles including the maintenance of membrane integrity and cell signaling. (Adapted from PMID:37279443).

Protein Function (UniProtKB)

A beta-1,4-glycosyltransferase that catalyzes the transfer of a mannose residue from GDP-mannose in a beta-1,4 linkage to glycosphingolipids (PubMed:12454022). Involved in the biosynthesis of arthroseries glycosphingolipids upstream of brn/brainiac; required for sequential elongation steps (PubMed:12454022, PubMed:15611100). Catalyzes addition of the second monosaccharide residue to form the dihexosylceramide glycolipid Man(beta1-4)Glc(beta1-1)Cer (MacCer) (PubMed:12454022). Highly specific for substrates containing a terminal beta-linked glucose residue (PubMed:12454022). Neurogenic protein implicated in epithelial development (PubMed:9012507). Required in the female germline for establishing and maintaining the follicular epithelium throughout oogenesis (PubMed:9012507). Collaborates with Notch on the apical surface of follicle cells to mediate germline-follicle cell adhesion (PubMed:9012507). Required, along with brn, in the lobula complex primordium for establishment of the lamina/lobula cortex boundary and correct targeting of photoreceptor (R-cell) neuron axons (PubMed:16182276). Downstream glycosphingolipid biosynthesis by beta4GalNAcTA is involved in motor coordination and locomotion (PubMed:17498683). Downstream glycosphingolipid biosynthesis by beta4GalNAcTB is involved in oocyte dorsal follicular cell fate specification by EGFR signaling (PubMed:17498683).

(UniProt, O01346)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

l(1)3Ae

Alleles tested [l(1)Ae2, l(1)Ae3, l(1)Ae6, l(1)Ae8, l(1)Ae10, l(1)Ae11] die in post puparial stage, but growth slowing starts at various earlier stages; tested alleles survive in hemizygous condition in gynandromorphs; hemizygous eyes rough. Pharate adults dissected from l(1)3Ae1 and l(1)Ae5 pupae show rough eyes, sparse microchaetae, and incomplete dorsal midline; XY but not XO males of both rescued by Dp(1;4)wm65g, exhibiting phenotype of dissected pharate adults described above (Shannon, Kaufman, Shen, and Judd, l972, Genetics 72: 615-38).

Summary (Interactive Fly)

glycosyltransferase - required for glycosphingolipid sugar chain elongation - affects extracellular Gurken gradiant by controlling Gurken planar transport in the extracellular space

Interactive Fly

Gene Model and Products

Number of Transcripts

3

Number of Unique Polypeptides

1

Please see the JBrowse view of Dmel\egh for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry O01346)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0070440

3303

457

FBtr0070439

3595

457

FBtr0340091

2946

457

Additional Transcript Data and Comments

Reported size (kB)

3.7 (northern blot)

(Goode et al., 1996)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

FBpp0070424

52.0

457

8.09

FBpp0070423

52.0

457

8.09

FBpp0309090

52.0

457

8.09

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

457 aa isoforms: egh-PB, egh-PC, egh-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

457 (aa); 52 (kD predicted)

(Goode et al., 1996)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\egh using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.42

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 5

organism | ubiquitous | uniform

(Soller et al., 2006)

embryonic stage 10 -- 14

presumptive embryonic/larval central nervous system

(Soller et al., 2006)

embryonic stage 15 -- larval stage

larval central nervous system

(Soller et al., 2006)

(Soller et al., 2006)

(Soller et al., 2006)

adult stage | male

(Ellis and Carney, 2011)

adult abdominal fat mass

(Ellis and Carney, 2011)

(Ellis and Carney, 2011)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Soller et al., 2006)

embryonic stage 1 -- 4

Comment: reference states 0-2 hr AEL

(Goode et al., 1996)

adult stage | female & oogenesis

germarium region 2b & germline cell

(Goode et al., 1996)

germarium region 2a & germline cell

(Goode et al., 1996)

adult stage & oogenesis stage S10

(Goode et al., 1996)

Additional Descriptive Data

All four egh transcripts are expressed at all developmental stages, with the 3.5kb transcript expressed at higher levels in adult females that have stored eggs. Maternally-supplied egh transcript is uniformly distributed in early embryos. In later embryos, egh transcripts become enriched in the developing CNS. Larval expression is also observed in the CNS, as well as in additional tissues including the imaginal discs. In adults, egh transcript is enriched in the head.

(Soller et al., 2006)

The in-situ localization of egh transcript is consistent with the proposed role of egh in epithelial morphogenesis during oogenesis. egh transcript is first detected during oogenesis in germ cells at the junction of germarium regions 2a and 2b. From stage S4 to S9, the egh transcript differentially accumulates in the oocyte. At stage S10, egh transcript expression declines in oocytes but increases in nurse cells. Subsequently, the content of nurse cells is transferred to oocytes. Northern analysis shows that the egh transcript is present in 0-2 hour embryos.

(Goode et al., 1996)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: P{egh-lacZ.S}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

presumptive embryonic/larval central nervous system

(Soller et al., 2006)

third instar larval stage

(Soller et al., 2006)

larval ventral nerve cord | restricted

(Soller et al., 2006)

(Soller et al., 2006)

adult ventral nerve cord

(Soller et al., 2006)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\egh in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\egh

View exonic expression by tissue for Dmel\egh

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31239/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001404

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001404

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001404

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9659

Flygut - An atlas of the Drosophila adult midgut

FBgn0001404

Images

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 53 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 12 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of egh

Transgenic constructs containing regulatory region of egh

Aberrations (Deficiencies and Duplications) ( 66 )

Inferred from experimentation ( 66 )

Gene disrupted in

(Nomura and Kurokawa, 1997)

(Goode et al., 1996, Williams et al., 1995)

(Thierry-Mieg, 1982, Judd et al., 1972)

(Kramers et al., 1983)

(Kramers et al., 1983)

(Thierry-Mieg, 1982, Judd et al., 1972)

(Nomura and Kurokawa, 1997)

(Nomura and Kurokawa, 1997)

(Kramers et al., 1991, Kramers et al., 1983, Lim and Snyder, 1974)

(Kramers et al., 1991, Kramers et al., 1983, Paradi et al., 1983, Thierry-Mieg, 1982, Liu and Lim, 1975, Lim and Snyder, 1974)

(Lim and Snyder, 1974)

(Kramers et al., 1991, Kramers et al., 1983, Lim and Snyder, 1974)

(Thierry-Mieg, 1982, Judd et al., 1972)

(Thierry-Mieg, 1982, Judd et al., 1972)

(Kramers et al., 1991, Kramers et al., 1983)

(Judd et al., 1972)

(Nomura and Kurokawa, 1997, Kramers et al., 1991, Kramers et al., 1983, Paradi et al., 1983, Thierry-Mieg, 1982, Liu and Lim, 1975, Lim and Snyder, 1974, Judd et al., 1972)

(Thierry-Mieg, 1982, Judd et al., 1972)

(Goldberg et al., 1989)

(Goldberg et al., 1989)

(Goldberg et al., 1989)

(Goldberg et al., 1989)

(Goldberg et al., 1989)

(Goldberg et al., 1989)

(Goldberg et al., 1989)

(Goldberg et al., 1989)

(Montgomery et al., 1991)

(Montgomery et al., 1991)

(Montgomery et al., 1991)

(Montgomery et al., 1991)

(Montgomery et al., 1991)

(Montgomery et al., 1991)

Gene duplicated in

(Kramers et al., 1991, Kramers et al., 1983, Judd et al., 1972)

(Popodi et al., 2010-, Venken et al., 2010)

(Judd et al., 1972)

Gene not disrupted in

(Judd et al., 1972)

(Thierry-Mieg, 1982, Judd et al., 1972)

(Lin and Wolfner, 1989, Thierry-Mieg, 1982, Judd et al., 1972)

(Lin and Wolfner, 1989, Thierry-Mieg, 1982, Judd et al., 1972)

(Thierry-Mieg, 1982, Judd et al., 1972)

(Nomura and Kurokawa, 1997)

(Nomura and Kurokawa, 1997)

(Kramers et al., 1991, Lin and Wolfner, 1989)

(Kramers et al., 1991)

(Kramers et al., 1991)

(Kramers et al., 1991)

(Schalet, 1986)

(Lim and Snyder, 1974)

(Kramers et al., 1991, Kramers et al., 1983, Paradi et al., 1983, Liu and Lim, 1975)

(Kramers et al., 1991, Kramers et al., 1983, Paradi et al., 1983, Liu and Lim, 1975, Lim and Snyder, 1974)

(Lin and Wolfner, 1989)

(Kramers et al., 1991)

(Lin and Wolfner, 1989, Thierry-Mieg, 1982, Judd et al., 1972)

(Judd et al., 1972)

(Goldberg et al., 1989)

(Goldberg et al., 1989)

(Goldberg et al., 1989)

(Goldberg et al., 1989)

(Goldberg et al., 1989)

(Goldberg et al., 1989)

Gene not duplicated in

(Kramers et al., 1991)

(Judd et al., 1972)

Dp(1;3)N^264-58

(Judd et al., 1972)

(Judd et al., 1972)

(Schalet, 1986)

(Judd et al., 1972)

Inferred from location ( 12 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | germline clone | maternal effect | recessive

egh²⁷

lethal | germline clone | neurogenic phenotype

egh²⁷

lethal | pharate adult stage | recessive

egh²⁷

lethal | pupal stage | recessive

egh²⁷

lethal | recessive

lethal - all die before end of P-stage | recessive

egh^62d18

lethal - all die before end of pupal stage | maternal effect

egh²⁷

lethal - all die before end of pupal stage | recessive

partially lethal - majority die

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

egh^GD3206

viable, with Scer\GAL4^pnr-MD237

egh^GD3206

Sterility

Allele

female sterile | germline clone | recessive

egh²⁷

Other Phenotypes

Allele

abnormal behavior

abnormal behavior (with egh⁷)

egh^cm

abnormal behavior (with egh^cm)

egh⁷

abnormal courtship behavior | male

abnormal courtship behavior | male | heat sensitive, with Scer\GAL4^ap-md544, Scer\GAL80^ts.αTub84B

egh^GD3206

abnormal courtship behavior | male | heat sensitive, with Scer\GAL4^elav-C155, Scer\GAL80^ts.αTub84B

egh^GD3206

abnormal neuroanatomy

abnormal neuroanatomy | larval stage, with Scer\GAL4^nSyb.PS

egh^UAS.Tag:MYC

abnormal neuroanatomy | larval stage, with Scer\GAL4^RapGAP1-OK6

egh^UAS.Tag:MYC

abnormal neuroanatomy | larval stage (with egh⁷)

egh^62d18

abnormal neuroanatomy | larval stage (with egh^62d18)

egh⁷

abnormal neuroanatomy | larval stage | recessive

egh^62d18

abnormal neuroanatomy | recessive | third instar larval stage

hyperplasia | recessive | third instar larval stage

egh^62d18

increased occurrence of cell division | germline clone

short lived, with Scer\GAL4^tsh-md621, egh⁷

egh^UAS.Tag:HA

short lived, with Scer\GAL4^tsh-md621, egh^UAS.Tag:HA

egh⁷

some die during pharate adult stage | recessive

egh^62d18

some die during pupal stage | maternal effect

egh²⁷

some die during pupal stage | recessive

egh^medley

Phenotype manifest in

Allele

abdominal tergite 1

abdominal tergite 2

abdominal tergite 3

abdominal tergite 4

abdominal tergite 5

abdominal tergite 6

abdominal tergite 7

dorsal appendage | germline clone

egh^medley

egg | germline clone

egh¹¹

egg chamber | germline clone

egg chamber | germline clone | maternal effect

egh²⁷

embryonic/larval midgut | embryonic stage

egh²⁷

embryonic/larval neuromuscular junction

egh^62d18

embryonic/larval neuromuscular junction, with Scer\GAL4^nSyb.PS

egh^UAS.Tag:MYC

embryonic/larval neuromuscular junction, with Scer\GAL4^RapGAP1-OK6

egh^UAS.Tag:MYC

embryonic/larval neuromuscular junction (with egh⁷)

egh^62d18

embryonic/larval neuromuscular junction (with egh^62d18)

egh⁷

embryonic/larval optic lobe

embryonic/larval plasmatocyte | ectopic

female germline cyst | germline clone

female reproductive system

egh¹¹

first optic chiasma

egh^cm

follicle cell | germline clone

egh⁷

lamina anlage glial cell

egh⁷

larval abdominal segment 9 | third instar larval stage

larval peripheral nervous system | third instar larval stage

larval ventral nerve cord (with egh⁷)

egh^cm

larval ventral nerve cord (with egh^cm)

egh⁷

larval ventral nerve cord | somatic clone

egh⁷

larval ventral nerve cord | third instar larval stage

egh⁷

lobula & neuron

egh⁷

nervous system | germline clone

egh²⁷

nurse cell | germline clone

oocyte | germline clone

oocyte | precursor

egh^cm

egh^cm

photoreceptor cell R1 & axon

photoreceptor cell R2 & axon

photoreceptor cell R3 & axon

photoreceptor cell R4 & axon

photoreceptor cell R5 & axon

photoreceptor cell R6 & axon

photoreceptor neuron

egh^cm

presumptive embryonic/larval nervous system | germline clone

procephalic segment

egh²⁵

stalk follicle cell

egh²⁵

stalk follicle cell | germline clone

subperineurial glial cell | increased number | third instar larval stage

egh^62d18

terminal bouton | larval stage

egh^62d18

terminal bouton | larval stage, with Scer\GAL4^nSyb.PS

egh^UAS.Tag:MYC

terminal bouton | larval stage, with Scer\GAL4^RapGAP1-OK6

egh^UAS.Tag:MYC

terminal bouton | larval stage (with egh⁷)

egh^62d18

terminal bouton | larval stage (with egh^62d18)

egh⁷

ventral denticle belt

egh²⁵

egh^medley

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (1)

13 of 14

Yes

Yes

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP000868

12 of 12

Yes

Yes

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:egh. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

ameliorates non-syndromic X-linked intellectual disability 63

modeled by Acsl⁰⁵⁸⁴⁷, Acsl^KO

(Huang et al., 2016)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Dahlgaard et al., 2012)

suppressible

(Dahlgaard et al., 2012)

suppressible

(Dahlgaard et al., 2012)

suppressible

(Dahlgaard et al., 2012)

enhanceable

(Dahlgaard et al., 2012)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Huang et al., 2018)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

57772

DroID - A comprehensive database of gene and protein interactions.

FBgn0001404

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

GLUCOSYLCERAMIDE BIOSYNTHESIS

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Dorso-ventral axis formation

Class of Gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

X

Recombination map

1-1

Cytogenetic map

Sequence location

X:2,588,503..2,599,111 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

3A6-3A6

Limits computationally determined from genome sequence between P{EP}CG32796^EP1385&P{EP}EP1160 and P{EP}egh^EP804

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

3A8-3A8

(Hollmann, 2000.2.9)

3A5-3A8

(determined by in situ hybridisation)

(Endow and Hatsumi, 1991)

Experimentally Determined Recombination Data

Location

1-1

(FlyBase, 2016)

1-1.0

(Comeron et al., 2012)

1-1.2

Left of (cM)

Right of (cM)

wds

(Judd et al., 1972)

Notes

Stocks and Reagents

Stocks (18)

Aberrations including deletions of this gene Aberrations including duplications of this gene

77890

w^*; P{UAS-egh.HA}2

600463

w^*; P{UASp-egh.P}3

28687

y¹ v¹; P{TRiP.JF03102}attP2

600462

w^*; P{UAS-egh.W}3/TM6B, Tb¹

15689

y¹ P{EPgy2}egh^EY03917 w^67c23

600466

egh^62d18 f^36a P{neoFRT}18A/FM6, w¹

107261

egh⁷/FM7a/Dp(1;2;Y)w⁺

124483

P{RS3}egh^UM-8329-3 w¹¹¹⁸

114134

P{GawB}egh^NP7190 w^* / FM7c

104847

y^* P{GawB}egh^NP5092 w^* / FM7c

v10137

w¹¹¹⁸ P{GD3206}v10137

v45160

w¹¹¹⁸; P{GD3206}v45160

More stocks available...

Genomic Clones (9)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (239)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

FI18161

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0001404

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0001404

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

egh is important for germ cell differentiation, for the integrity of the cortical actin cytoskeleton of the germ cells and for the establishment of a correct anteroposterior axis of the egg chamber.

(Rubsam et al., 1998)

Phenotypic analysis proposes that brn and egh, originating from the germline, collaborate with N on the apical surface of follicle cells to mediate germline-follicle cell adhesion. Wild type function of egh and brn is required for the formation of the follicular epithelium and maintenance of the apical-basal polarity, to inhibit follicle cell division, induction of dorsal follicle cell fates (but not specification of polar/stalk cell fates) and for concerted border cell-main body epithelium migration.

(Goode et al., 1996)

egh encodes a transmembrane protein.

(Schafer, 1995.9.19)

The egh and Egfr gene products cooperate in the same pathway during oogenesis and eye development. egh acts upstream of Egfr in the germ line. egh and Dl interact in the development of the embryonic nervous system.

(Simmerl et al., 1995)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: egh CG9659

(Nusskern, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

CG9659

(Yamamoto-Hino et al., 2015, Haelterman et al., 2014, Ellis and Carney, 2011, Perkins et al., 2009, Saleh et al., 2006, Norga et al., 2003, Benos, 2000.12.13)

EG:BACR25B3.8

(Benos et al., 2001, Benos, 2000.2.6, Benos, 2000.12.13)

Egh

(Xiao et al., 2025, Nishihara, 2020, Yamamoto-Hino et al., 2015)

ZW-4

(Paradi et al., 1983)

l(1)3Ae

l(1)zw4

(Rubsam et al., 1997, Williams et al., 1995, Williams et al., 1992, Endow and Hatsumi, 1991, Lin and Wolfner, 1989, Garcia-Bellido and Robbins, 1983, Paradi et al., 1983, Lim and Snyder, 1974, Shannon et al., 1972)

zw-4

(Nomura and Kurokawa, 1997, Kramers et al., 1991, Kramers et al., 1983, Liu and Lim, 1975)

zw4

(Williams et al., 1997, Goode et al., 1996, Melnick, 1995.2.17, Montgomery et al., 1991, Goldberg et al., 1989, Lefevre and Watkins, 1986, Schalet, 1986, Robbins, 1983, Thierry-Mieg, 1982, Lefevre, 1981, Judd et al., 1972)

β1,4ManT

(Yamamoto-Hino et al., 2015)

Name Synonyms

Egghead

(Nishihara, 2020, Wang et al., 2016, Dahlgaard et al., 2012)

egghead

(Satoh et al., 2013, Ellis and Carney, 2011, Kraut, 2011, Moran and Kyriacou, 2009, Pizette et al., 2009, Saleh et al., 2009, Beckstead et al., 2007, Chen et al., 2007, Saleh et al., 2006, Soller et al., 2006, Demakov et al., 2004)

zeste-white 4

(Melnick, 1995.2.17, Melnick, 1994.10.6)

β1,4-mannosyltransferase

(Nishihara, 2020)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 50 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31239

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

O01346

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

O01346

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q95ST6

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

O01346

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31239/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001404

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001404

FlyMine - An integrated database for Drosophila genomics

FBgn0001404

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG9659

MARRVEL_MODEL - MARRVEL (model organism gene)

31239

Linkouts

BioGRID - A database of protein and genetic interactions.

57772

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0001404

DroID - A comprehensive database of gene and protein interactions.

FBgn0001404

DRSC - Results frm RNAi screens

FBgn0001404

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001404

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0001404

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9659

Flygut - An atlas of the Drosophila adult midgut

FBgn0001404

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC008154

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Dorso-ventral axis formation

References (119)