FlyBase Gene Report: Dmel\crp

General Information

Symbol

Dmel\crp

Species

D. melanogaster

Name

cropped

Annotation Symbol

CG7664

Feature Type

protein_coding_gene

FlyBase ID

FBgn0001994

Gene Model Status

Current

Stock Availability

39 publicly available

Gene Summary

cropped (crp) encodes a transcription factor that controls cellular branching of the terminal cells at the ends of tracheal tubes. As a downstream target gene of the product of Myc, it affects cell growth, organ size and survival. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

dAP-4, l(2)35Fd, AP-4, BG:DS02740.3

Key Links

Genomic Location

Cytogenetic map

35F1-35F1

Sequence location

2L:16,263,556..16,287,730 [-]

Recombination map

2-52

RefSeq locus

NT_033779 REGION:16263556..16287730

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables myosin binding

inferred from physical interaction with FLYBASE:Myo10A; FB:FBgn0263705

(Liu et al., 2008)

enables sequence-specific DNA binding

inferred from direct assay

(King-Jones et al., 1999)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables DNA-binding transcription factor activity, RNA polymerase II-specific

inferred from biological aspect of ancestor with PANTHER:PTN000761437

(GO Reference Genome Project, 2011-)

enables protein dimerization activity

inferred from electronic annotation with InterPro:IPR011598, InterPro:IPR036638

(InterPro Project Members, 2004-)

enables RNA polymerase II cis-regulatory region sequence-specific DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN000761437

(GO Reference Genome Project, 2011-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in larval somatic muscle development

inferred from expression pattern

(Dobi et al., 2014)

inferred from mutant phenotype

(Dobi et al., 2014)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN000761437

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Wong et al., 2015, King-Jones et al., 1999)

inferred from high throughput direct assay

(Lye et al., 2014)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN001039502

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS -: Basic helix-loop-helix (bHLH) transcription factors are sequence-specific DNA-binding proteins that regulate transcription. They are characterized by a 60 amino acid region comprising a basic DNA binding domain followed by a HLH motif formed from two amphipathic α-helices connected by a loop. bHLH transcription factors form homo- and hetero-dimeric complexes, which bind to a E box consensus sequence. (Adapted from PMID:15186484).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\crp for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V406)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.52

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

crp-RA

FBtr0080854

NM_057257

3537

631

crp-RB

FBtr0343814

NM_001299047

4402

631

Additional Transcript Data and Comments

Reported size (kB)

3.867 (longest cDNA)

(King-Jones et al., 1999)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

crp-PA

FBpp0080411

67.2

631

5.99

FBpp0310368

67.2

631

5.99

Q9V406

NP_001285976

AHN54490

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

631 aa isoforms: crp-PA, crp-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

631 (aa); 85 (kD observed); 67 (kD predicted)

(King-Jones et al., 1999)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\crp using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.00

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 5

organism | ubiquitous

(Wong et al., 2015)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 8 -- 15

larval central nervous system

(Wong et al., 2015)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11

embryonic/larval gut

(Wong et al., 2015)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

gonad

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

third instar larval stage

larval central nervous system

Comment: high level

(Wong et al., 2015)

imaginal disc

Comment: high level

(Wong et al., 2015)

larval pericardial cell

(Wong et al., 2015)

larval cardial cell

(Wong et al., 2015)

embryonic/larval fat body

Comment: low level

(Wong et al., 2015)

embryonic/larval salivary gland

Comment: low level

(Wong et al., 2015)

embryonic/larval tracheal section

Comment: low level

(Wong et al., 2015)

embryonic/larval plasmatocyte

Comment: low level

(Wong et al., 2015)

embryonic/larval epidermis

Comment: low level

(Wong et al., 2015)

embryonic/larval midgut

(Wong et al., 2015)

gastric caecum

(Wong et al., 2015)

histoblast

(Wong et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

organism | ubiquitous

(King-Jones et al., 1999)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(King-Jones et al., 1999)

third instar larval stage

• nucleus

(King-Jones et al., 1999)

salivary gland

• nucleus

(King-Jones et al., 1999)

Additional Descriptive Data

Strong nuclear staining is observed in all third instar larval tissues examined.

(King-Jones et al., 1999)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Wong et al., 2015, King-Jones et al., 1999)

inferred from high throughput direct assay

(Lye et al., 2014)

Expression Deduced from Reporters

Reporter: P{PZ}crp⁰⁰²³²

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

embryonic/larval tracheal section

(Meister and Braun, 1995.10)

imaginal disc

(Meister and Braun, 1995.10)

larval brain

(Meister and Braun, 1995.10)

embryonic/larval fat body

(Meister and Braun, 1995.10)

adult stage

third segment of antenna

(Vosshall, 1998.8.10)

photoreceptor cell R1 -- photoreceptor cell R8

(Vosshall, 1998.8.10)

Reporter: PBac{CPTI-un}crp^CPTI002760

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain cell body rind

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\crp in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\crp

View exonic expression by tissue for Dmel\crp

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0001994

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34956/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001994

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001994

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001994

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7664

Flygut - An atlas of the Drosophila adult midgut

FBgn0001994

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\crp

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 75 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 15 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of crp

Transgenic constructs containing regulatory region of crp

Aberrations (Deficiencies and Duplications) ( 61 )

Inferred from experimentation ( 61 )

Gene not disrupted in

Df(2L)64j

(Ashburner et al., 1990)

Df(2L)A72

(Ashburner et al., 1990)

Df(2L)A267

(Ashburner et al., 1990)

Df(2L)ARR1

(Ashburner et al., 1990)

Df(2L)b80e4

(Ashburner et al., 1990)

Df(2L)cact-255rv64

(Spradling et al., 1999, Dawson et al., 1995, BDGP Project Members, 1994-1999)

Df(2L)el20

(Ashburner et al., 1990)

Df(2L)el28

(Ashburner et al., 1990)

Df(2L)fn12

(Ashburner et al., 1990)

Df(2L)fn26

(Ashburner et al., 1990)

Df(2L)fn27

(Ashburner et al., 1990)

Df(2L)H20

(Ashburner et al., 1990)

Df(2L)H60-3

(Dawson et al., 1995)

Df(2L)II30

(Roote, 1996.8)

Df(2L)n78l3

(Ashburner et al., 1990)

Df(2L)noc11

(Ashburner et al., 1990)

Df(2L)osp29

(Eberl et al., 1998)

Df(2L)osp38

(Ashburner et al., 1990)

Df(2L)RA5

(Ashburner et al., 1990)

Df(2L)rd9

(Ashburner et al., 1990)

Df(2L)Sco7

(Ashburner et al., 1990)

Df(2L)Sco^rv16

(Ashburner et al., 1990)

Df(2L)Sco^rv18

(Ashburner et al., 1990)

Df(2L)Sco^rv19

(Ashburner et al., 1990)

Df(2L)Sco^rv25

(Ashburner et al., 1990)

Df(2L)SS1

(Ashburner et al., 1990)

Df(2L)TE35BC-3

(Ashburner et al., 1990)

Df(2L)TE35BC-4

(Ashburner et al., 1990)

Df(2L)TE35BC-34

(Ashburner et al., 1990)

Df(2L)TE35D-1

(Ashburner et al., 1990)

Df(2L)TE35D-2

(Ashburner et al., 1990)

Df(2L)TE35D-3

(Ashburner et al., 1990)

Df(2L)TE35D-4

(Ashburner et al., 1990)

Df(2L)TE35D-6

(Ashburner et al., 1990)

Df(2L)TE35D-13

(Ashburner et al., 1990)

Df(2L)TE35D-14

(Eberl et al., 1998, Ashburner et al., 1990)

Df(2L)TE35D-15

(Ashburner et al., 1990)

Df(2L)TE35D-19

(Ashburner et al., 1990)

Df(2L)TE35D-20

(Ashburner et al., 1990)

Df(2L)TE35D-22

(Ashburner et al., 1990)

Df(2L)TW50

(BDGP Project Members, 1994-1999)

In(2L)dpp^s22Ldpp^d36R

(Ashburner et al., 1990)

In(2L)el18

(Eberl et al., 1998, Dawson et al., 1995, Ashburner et al., 1990)

In(2LR)Sco^rv1-DV7

(Ashburner et al., 1990)

T(2;3)Sco^rv7

(Ashburner et al., 1990)

Gene partially disrupted in

Df(2L)k08106-rv6

(Roote, 1999.7.7)

Df(2L)k08106-rv15

(Roote, 1999.7.7)

Gene duplicated in

Dp(2;3)osp³

(Ashburner et al., 1990)

Gene not duplicated in

Dp(2;1)Sco^rv23

(Ashburner et al., 1990)

Dp(2;2)GYL

(Ashburner et al., 1990)

Dp(2;2)TE35B-54^L

(Ashburner et al., 1990)

In(2L)C163.41^LC158^R

(Ashburner et al., 1990)

In(2L)Sco^rv17LC158^R

(Ashburner et al., 1990)

Inferred from location ( 3 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

lethal | male limited, with Scer\GAL4^Bx-MS1096

crp^KK108184

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

crp^GD13194

partially lethal - majority die, with Scer\GAL4^phtm.PO

crp^KK108184

partially lethal - majority die, with Scer\GAL4^pnr-MD237

crp^GD13194

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

crp^GD13194

viable, with Scer\GAL4^tin.CΔ4

crp^GD13194

Sterility

Allele

fertile

Other Phenotypes

Allele

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

crp^GD13194

decreased cell number, with Scer\GAL4^A9

crp^DN.UAS

decreased cell size, with Scer\GAL4^A9

crp^DN.UAS

increased cell death, with Scer\GAL4^A9

crp^DN.UAS

increased cell size, with Scer\GAL4^btl.PS

crp^UAS.W

some die during pupal stage, with Scer\GAL4^pnr-MD237

crp^GD13194

visible

crp^k08106b

visible, with Scer\GAL4^bbg-C96

crp^d04745

visible, with Scer\GAL4^Bx-MS1096

crp^{UAS.ORF.GW.Tag:HA}

visible, with Scer\GAL4^pnr-MD237

crp^GD13194

visible | female limited, with Scer\GAL4^Bx-MS1096

crp^KK108184

visible | recessive

Phenotype manifest in

Allele

dorsal tracheal branch primordium

crp^k10415

embryonic/larval dorsal tracheal branch

crp^k10415

embryonic/larval dorsal tracheal branch 7

crp^DN.UAS

embryonic/larval fat body, with Scer\GAL4^A9

crp^UAS.W

embryonic/larval ganglionic tracheal branch

crp^k10415

embryonic/larval salivary gland, with Scer\GAL4^A9

embryonic/larval tracheal dorsal trunk, with Scer\GAL4^btl.PS

crp^DN.UAS

embryonic/larval tracheal section

embryonic/larval tracheal section, with Scer\GAL4^bs-1348

crp^DN.UAS

embryonic/larval tracheal section, with Scer\GAL4^btl.PS

embryonic/larval tracheal section (with crp^k00809)

crp^k10415

embryonic/larval tracheal section (with crp^k10415)

crp^k00809

embryonic/larval tracheal section (with Df(2L)r10)

embryonic/larval visceral tracheal branch

crp^k10415

eye

crp²

interommatidial bristle | posterior, with Scer\GAL4^GMR.PU

larval brain, with Scer\GAL4^A9

crp^UAS.W

larval lateral G tracheal branch

crp^k10415

larval lateral G tracheal branch, with Scer\GAL4^bs-1348

crp^DN.UAS

larval lateral G tracheal branch, with Scer\GAL4^btl.PS

crp^DN.UAS

larval somatic muscle cell

crp^RAR46

larval somatic muscle cell (with Df(2L)BSC278)

crp^RAR46

larval ventral nerve cord, with Scer\GAL4^A9

crp^UAS.W

leg

crp²

mesonotum, with Scer\GAL4^pnr-MD237

crp^GD13194

ommatidium, with Scer\GAL4^GMR.PU

crp^UAS.W

ommatidium | posterior, with Scer\GAL4^GMR.PU

crp^DN.UAS

terminal tracheal cell, with Scer\GAL4^btl.PS

crp^DN.UAS

tracheal lumen

crp^k10415

trichogen cell, with Scer\GAL4^pnr-MD237

crp^GD13194

wing

wing, with Scer\GAL4^bbg-C96

crp^d04745

wing, with Scer\GAL4^Bx-MS1096

crp^{UAS.ORF.GW.Tag:HA}

wing | female limited, with Scer\GAL4^Bx-MS1096

crp^KK108184

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (62)

Hsap\TFAP4

10 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (40)

Rnor\Tfap4

10 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (44)

Mmus\Tfap4

9 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (34)

Xtro\tfap4

8 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\neurod4

1 of 13

Yes

Xtro\neurod6

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (51)

Drer\tfap4

9 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:ch211-196c10.13

1 of 14

Drer\si:ch211-246m6.4

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (21)

Cele\hlh-11

8 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (28)

Agam\AgaP_AGAP010201

10 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP013301

1 of 12

Agam\AgaP_AGAP013335

1 of 12

Agam\AgaP_AGAP013406

1 of 12

Arabidopsis thaliana (thale-cress) (35)

Atha\bHLH38

2 of 13

Yes

Atha\bHLH39

2 of 13

Yes

Atha\BHLH100

2 of 13

Yes

Atha\BHLH101

2 of 13

Yes

Atha\AIB

1 of 13

Atha\AT1G01260

1 of 13

Atha\AT1G05805

1 of 13

Yes

Atha\AT1G10120

1 of 13

Atha\AT1G12540

1 of 13

Yes

Atha\AT1G62975

1 of 13

Yes

Atha\AT1G71200

1 of 13

Yes

Atha\AT2G22760

1 of 13

Atha\AT3G07340

1 of 13

Atha\AT3G61950

1 of 13

Yes

Atha\AT4G16430

1 of 13

Atha\AT4G25400

1 of 13

Yes

Atha\AT4G25410

1 of 13

Yes

Atha\AT4G37850

1 of 13

Atha\AT5G51780

1 of 13

Yes

Atha\AT5G51790

1 of 13

Yes

Atha\AT5G56960

1 of 13

Atha\ATMYC1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\SPAC3F10.12c

1 of 12

Yes

Spom\sre2

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:crp. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (28)

bigmax

3 of 13

Mondo

3 of 13

cato

2 of 13

1 of 13

amos

1 of 13

ato

1 of 13

cwo

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Hey

1 of 13

HLH3B

1 of 13

HLH4C

1 of 13

HLH54F

1 of 13

Max

1 of 13

Mnt

1 of 13

nau

1 of 13

net

1 of 13

Oli

1 of 13

sage

1 of 13

soul

1 of 13

tap

1 of 13

twi

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

crp^EY07016

exacerbates tauopathy

modeled by Hsap\MAPT^GMR.Ex.PJ

(Ambegaokar and Jackson, 2011)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TFAP4; transcription factor AP-4

10 of 14

600743

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

crp - Myo10A

pull down, autoradiography, two hybrid

(Liu et al., 2008)

crp - Ubx

anti tag coimmunoprecipitation, anti tag western blot, proximity-dependent biotin identification, Identification by mass spectrometry

(Carnesecchi et al., 2020)

crp - cg

anti bait coimmunoprecipitation, western blot, Identification by mass spectrometry

(Chetverina et al., 2022)

crp - z

anti bait coimmunoprecipitation, Identification by mass spectrometry, western blot

(Chetverina et al., 2022)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

60968

DroID - A comprehensive database of gene and protein interactions.

FBgn0001994

MIST (protein-protein) - An integrated Molecular Interaction Database

34956

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-52

Cytogenetic map

35F1-35F1

Sequence location

2L:16,263,556..16,287,730 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

35F1-35F1

Limits computationally determined from genome sequence between P{lacW}CycE^k05007&P{lacW}CycE^k02602 and P{EP}crp^EP721&P{lacW}crp^k07829

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

35E4-35E4

(Oh et al., 2003)

35F1-35F2

(King-Jones, 1999.6.11, Chiu and Krasnow, 1997)

35F1-35F2

(determined by in situ hybridisation) 35F4--5 (determined by in situ hybridisation) 35F11--12 (determined by in situ hybridisation)

(Ashburner et al., 1999)

35F1-35F2

(determined by in situ hybridisation)

(Spradling et al., 1999, Chiu, 1997.4.18)

35F11-35F12

(determined by in situ hybridisation)

(Beaton and Laverty, 1998.3.6)

31F1-31F2

35F1--2 35F11--12 35F4--5

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-52

(FlyBase, 2016)

2-49.3

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (39)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

63149

w^*; P{UAS-crp.W}168

63150

w^*; P{UAS-crp.DN}238

43494

w^*; P{GSV1}crp^s-250

48240

w¹¹¹⁸; P{GMR10B02-GAL4}attP2

49234

w¹¹¹⁸; P{GMR10B01-GAL4}attP2

68931

w¹¹¹⁸; P{R10B01-p65.AD}attP40

69951

w¹¹¹⁸; P{R10B02-p65.AD}attP40

69952

w¹¹¹⁸; P{R10B02-GAL4.DBD}attP2

FlyORF

F001873

M{UAS-crp.ORF.3xHA.GW}ZH-86Fb

F004891

M{UAS-crp.ORF-VNshort}ZH-86Fb

VDRC

v100565

P{KK108184}VIE-260B

v26886

w¹¹¹⁸; P{GD13194}v26886

More stocks available...

Genomic Clones (37)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (154)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0001994

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0001994

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Chetverina et al., 2022, Wong et al., 2015, King-Jones et al., 1999)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

1 allele of crp recovered in a P-insertion screen.

(Oh et al., 2003)

Area matching Drosophila ESTs AA948907 and AA92191.

(Davies, 2001.3.15)

Identified as a gene with significant level of mRNA cycling as assessed by expression analysis using high density oligonucleotide arrays with probe generated from adult heads harvested over six time points over the course of a day.

(McDonald and Rosbash, 2001)

da and crp are two independent DNA-binding components of Sebp3, a protein complex that appears to be required for the transcriptional activation of Sgs4. Since Sebp3 binding activity is specific for the salivary gland, and da and crp are ubiquitously expressed, the Sebp3 complex probably contains additional protein(s). In direction of increasing cytology: twe- crp- pkaap+

(King-Jones et al., 1999)

Mutants isolated in a screen of the second chromosome identifying genes affecting disc morphology.

(Roch et al., 1998)

Expression pattern studies and structure of the predicted gene product suggest crp may control terminal branching by regulating the expression of bnl or other tracheogenic factors.

(Chiu and Krasnow, 1997)

1 additional allele is discussed but is not named.

(Ashburner et al., 1990)

Relationship to Other Genes

Source for database merge of

Source for merge of: crp l(2)k03505

(Roote, 1998.11.16)

Source for merge of: crp MS:35F.AT17

(FlyBase, 1996-)

Additional comments

Source for merge of crp MS:35F.AT17 was sequence comparison ( date:040910 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: crp CG7664

(Kodira, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (18)

Reported As

Symbol Synonym

35F(AT)₁₇

(Flores and Engels, 1999)

AP-4

(King-Jones, 1999.6.11)

AP4

(Li and White, 2003)

BG:DS02740.3

(Ashburner et al., 1999, Ashburner et al., 1999.10.12)

CG7664

(National Institute of Genetics Fly Stocks, 2022-, Hosono et al., 2015, Schertel et al., 2013, Ni et al., 2011.7.19, Keleman et al., 2009.8.5, Perkins et al., 2009, Liu et al., 2008, Stevens et al., 2008, Stevens et al., 2008, Molnar et al., 2006)

CRP

(Zhimulev et al., 2024, Carnesecchi et al., 2020)

Crp

(Carnesecchi et al., 2020)

MS:35F.AT17

bHLHe63

(Stevens et al., 2008)

crp

(Liu et al., 2024, Yheskel et al., 2023, Quiquand et al., 2021, Zhang et al., 2021, Sigalova et al., 2020, Wang et al., 2020, Zhao et al., 2020, Best, 2019, Hall et al., 2019, Bischof et al., 2018, Yeung et al., 2018, Ables et al., 2016, Atkins et al., 2016, Clandinin and Owens, 2016-, Gene Disruption Project members, 2015-, Nagarkar-Jaiswal et al., 2015, Schertel et al., 2015, Sopko et al., 2015, Van Bortle et al., 2015, Wong et al., 2015, Ashwal-Fluss et al., 2014, Dobi et al., 2014, Lye et al., 2014, Bonke et al., 2013, de Celis et al., 2013.9.11, Kwon et al., 2013, Moshkin et al., 2013, Schertel et al., 2013, Sen et al., 2013, Antao et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Spokony, 2012.12.12, Abruzzi et al., 2011, Ambegaokar and Jackson, 2011, Friedman et al., 2011, Levis et al., 2011.12.16, Blanco et al., 2010, Meadows et al., 2010, Buszczak et al., 2007, Christensen and Cook, 2007.5.8, Grieder et al., 2007, Molnar et al., 2006, Moorman et al., 2006, Liebl and Featherstone, 2005)

dAP-4

(Halme et al., 2008, King-Jones et al., 1999, King-Jones et al., 1999)

l(2)00232

l(2)35Fd

(Ashburner et al., 1999, Eberl et al., 1998)

l(2)SH1614

l(2)SH2 1614

(Oh et al., 2002.3.26)

l(2)k00809

(Chen et al., 1998)

l(2)k03505

Name Synonyms

cropped

(Atkins et al., 2016, Wong et al., 2015, Bonke et al., 2013, Ambegaokar and Jackson, 2011, Meadows et al., 2010, Gerhold et al., 2008, Halme et al., 2008, Liu et al., 2008, Kankel et al., 2007, Chan et al., 2005)

Secondary FlyBase IDs

FBgn0024216
FBgn0026860
FBgn0041143
FBgn0065396

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 85 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34956

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V406

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9V406

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0001994

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34956/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001994

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001994

FlyMine - An integrated database for Drosophila genomics

FBgn0001994

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG7664

MARRVEL_MODEL - MARRVEL (model organism gene)

34956

Linkouts

BioGRID - A database of protein and genetic interactions.

60968

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0001994

DroID - A comprehensive database of gene and protein interactions.

FBgn0001994

DRSC - Results frm RNAi screens

FBgn0001994

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001994

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0001994

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7664

Flygut - An atlas of the Drosophila adult midgut

FBgn0001994

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC007652

MIST (protein-protein) - An integrated Molecular Interaction Database

34956

References (149)

The following external sites may use different assemblies or annotations than FlyBase.

-1.00

Report Sections