Gene model reviewed during 5.46
There is only one protein coding transcript and one polypeptide associated with this gene
2616 (aa); 292 (kD predicted)
Requires cleavage for activation (presumably).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ndl using the Feature Mapper tool.
ndl transcripts are expressed almost exclusively in follicle cells. They are first detected at stage S7 of oogenesis but reach a peak at stages S9 and S10. In the majority of egg chambers, the transcript is asymmetrically distributed with a higher level seen in ventral than in dorsal follicle cells. The transcript decreases in abundance dramatically in late stage S10 and is undetectable by stage S11.
GBrowse - Visual display of RNA-Seq signalsView Dmel\ndl in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: ndl CG10129
The non-protease domains of ndl do not influence the signaling pathway that establishes embryonic DV polarity.
ndl has an integral role in the biogenesis of the vitelline membrane and may function in embryonic dorsal ventral patterning through creation of a matrix structure necessary for activity of the dorsal ventral protease cascade.
Catalytic activity of the protease domain of the modular ndl protein is required for dorsoventral patterning of the embryo.
The serine protease activity of ndl protein is essential for embryonic dorsoventral polarity. Active ndl protease is generated by autoproteolytic cleavage of a zymogen form. This activation is independent of the other known proteases involved in establishment of dorsoventral polarity and appears to occur symmetrically on the surface of the embryo.
A follicular cell marker system that yields a visible phenotype within the mature egg shell allows direct comparison of a clone and its effect on the dorsal ventral pattern of the embryos. Follicular epithelium has a lack of a localised requirement for ndl protease activity.
Phenotypic and genetic analysis reveals that ndl has two distinct roles important for embryogenesis: one in establishing dorsoventral polarity, the other in ensuring structural integrity of the egg.
Local activation of the protease cascade within the extra embryonic perivitelline compartment that defines embryonic dorsoventral polarity depends on a positional cue that is laid down during oogenesis outside the oocyte, ndl encodes an essential component of this cue. ndl function is required during oogenesis and also during the early stages of embryogenesis.
Double mutant combinations of ndl with ea alleles demonstrate that spatial regulation of ea activity by localized zymogen activation is a key initial event in defining the polarity of the dorsal-ventral embryonic pattern.