transcription factor - zinc finger - pair rule gene - required for the timely activation of and during segmentation - controls frequency doubling in Drosophila segmentation by altering the pair-rule gene regulatory network
Gene model reviewed during 5.49
Stop-codon suppression (UGA) postulated; FBrf0243886.
Gene model reviewed during 6.32
None of the polypeptides share 100% sequence identity.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\opa using the Feature Mapper tool.
opa is expressed in a broad domain encompassing the entire segmented region of the embryo during stages 5-9. At stage 5, expression extends from 16%-78% egg length in ventrally located cells fated to become mesoderm and in dorsally located ectodermal cells. During gastrulation, expression becomes periodic, showing 14 stripes. By late stage 9, expression is no longer seen in the ectoderm or the mesoderm with the exception of a small number of cells in the neurogenic region, the tip of the germ band, and the head. Late in stage 11, opa expression initiates in the visceral mesoderm in seven discrete clusters of cells, arranged in two groups. In stage 12, expression becomes continuous in each region yielding at stage 13, two distinct domains of expression in the visceral mesoderm. The anterior domain corresponds to parasegment 5 and the posterior domain to parasegments 9-12. During stage 15, the 2nd midgut constriction forms between the opa expression domains. At stage 16, the first midgut constriction forms at the posterior boundary of the anterior opa expression domain and the third midgut constriction forms within the anterior margin of the posterior opa expression domain.
opa transcripts are detected in embryonic, larval, and pupal stages with peak levels between 2 and 12 hours of embryogenesis. opa transcripts are first detected in early stage 5 embryos in a 10 cell wide stripe at about 80% egg length. Expression extends posteriorly and becomes uniform over the region from 20% to 80% egg length with the posterior border being more sharply defined than the anterior border. Expression continues in this domain through gastrulation. During germ band extension, a pattern of 14 stripes develops over a low background level of expression.
GBrowse - Visual display of RNA-Seq signalsView Dmel\opa in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: opa CG1133
One of 42 Drosophila genes identified as being most likely to reveal molecular and cellular mechanisms of nervous system development or plasticity relevant to human Mental Retardation disorders.
Mutants exhibit pair-rule pattern defects.
opa is required for formation of the three characteristic midgut constrictions. At the locations of the first and third constrictions, opa is positively regulated by Antp and abd-A, respectively, and negatively regulated by Ubx and dpp.
Deletes alternate metasegments, as defined by a line separating the anterior from the posterior parts of the anterior compartments; portions of denticle bands of T2, A1, A3, A5 and A7 and naked cuticle of T3, A2, A4 and A6 missing.
Mutations in zygotic pair rule gene opa interact with RpII140wimp.
Genetic analysis demonstrates that opa is dispensable for efficient homeotic gene expression in the visceral mesoderm.