FlyBase Gene Report: Dmel\pip

General Information

Symbol

Dmel\pip

Species

D. melanogaster

Name

pipe

Annotation Symbol

CG9614

Feature Type

protein_coding_gene

FlyBase ID

FBgn0003089

Gene Model Status

Current

Stock Availability

15 publicly available

Gene Summary

pipe (pip) encodes a sulfotransferase that contributes to the formation and polarity of the embryonic dorsal-ventral axis. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

PIP2

Key Links

Genomic Location

Cytogenetic map

76A5-76A6

Sequence location

3L:19,298,623..19,337,609 [-]

Recombination map

3-45

RefSeq locus

NT_037436 REGION:19298623..19337609

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (14 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

enables endopeptidase activator activity

inferred from mutant phenotype

(Cho et al., 2010)

enables protein binding

inferred from physical interaction with UniProtKB:O44342

(Barnewitz et al., 2004)

enables sulfotransferase activity

inferred from direct assay

(Zhang et al., 2009)

inferred from mutant phenotype

(Zhu et al., 2005)

NOT enables chondroitin sulfotransferase activity

inferred from direct assay

(Xu et al., 2007)

NOT enables heparan sulfate sulfotransferase activity

inferred from direct assay

(Xu et al., 2007)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables sulfotransferase activity

inferred from electronic annotation with InterPro:IPR005331, InterPro:IPR007734

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000951070

(GO Reference Genome Project, 2011-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in dorsal/ventral axis specification

inferred from genetic interaction with FLYBASE:ea; FB:FBgn0000533

(Chasan et al., 1992)

inferred from mutant phenotype

(Cho et al., 2010, Zhang et al., 2009, Ligoxygakis et al., 2003, Sen et al., 2000)

inferred from genetic interaction with FLYBASE:gd; FB:FBgn0000808

(Cho et al., 2012, Sen et al., 2000)

inferred from genetic interaction with FLYBASE:ndl; FB:FBgn0002926

(Sen et al., 2000)

inferred from genetic interaction with FLYBASE:wbl; FB:FBgn0004003

(Sen et al., 2000)

involved_in embryonic pattern specification

inferred from mutant phenotype

(Zhu et al., 2005)

involved_in regulation of multicellular organism growth

inferred from mutant phenotype

(Zhu et al., 2005)

involved_in Toll receptor ligand protein activation cascade

inferred from mutant phenotype

(Cho et al., 2010, Ligoxygakis et al., 2003)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in chromosome

inferred from direct assay

(Sen et al., 2000)

located_in Golgi apparatus

inferred from direct assay

(Sen et al., 2000)

located_in nucleus

inferred from direct assay

(Sen et al., 2000)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in Golgi membrane

non-traceable author statement

(Sen et al., 2000)

located_in membrane

inferred from electronic annotation with InterPro:IPR005331, InterPro:IPR007734

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

DORSAL GROUP

UNCLASSIFIED SULFOTRANSFERASES

Pathway (FlyBase)

EXTRACELLULAR SPATZLE ACTIVATING PATHWAY CORE COMPONENTS

Protein Family (UniProt)

Belongs to the sulfotransferase 3 family. (Q86BJ3)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

pipe (pip) encodes a sulfotransferase that contributes to the formation and polarity of the embryonic dorsal-ventral axis. [Date last reviewed: 2019-03-14]

Gene Group (FlyBase)

DORSAL GROUP -: The Dorsal group genes encode components of the Toll pathway and were initially identified by maternal effect mutations resulting in the dorsalization of the embryo. (Adapted from FBrf0091014 and FBrf0223077).
UNCLASSIFIED SULFOTRANSFERASES -: This group comprises sulfotransferases that do not classify under other groups in FlyBase.

Pathway (FlyBase)

EXTRACELLULAR SPATZLE ACTIVATING PATHWAY CORE COMPONENTS -: In Drosophila, activation of the Toll pathway is controlled by the generation of a cleaved, active, Toll-binding form of spatzle ligand. Proteolytic activation of spatzle ligand lies downstream of several zymogen activation cascades that are initiated by different cues. In dorsal-ventral patterning, localized activation of spz results in ventral nuclear accumulation of dl. During viral and fungal and gram positive bacterial immune challenge, zymogen cascades are activated by extracellular pattern recognition receptors or virulence factor-mediated cleavage of the zymogen persephone (psh). (Adapted from FBrf0091014 and FBrf0223077).

Protein Function (UniProtKB)

Sulfotransferase involved in dorsoventral axis patterning in early embryos (PubMed:9568717, PubMed:9827800). Required for the ventral activation of ea/easter by the protease snk in the perivitelline space between the embryonic membrane and the eggshell; activation of ea requires both activation of the ndl-gd-snk protease cascade and sulfation of a vitelline membrane component by pip (PubMed:16566925, PubMed:20605458, PubMed:9827800). Probably acts by mediating the sulfation of some glycoprotein or glycosaminoglycan stably deposited in the vitelline membrane, whose ventrally localized modification leads to spatially restricted activation of the protease cascade resulting in localized activation of the spz Toll receptor ligand by ea (Probable).

(UniProt, Q86BJ3)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

pip: pipe

Maternal-effect lethal; homozygous females are sterile. As in the mutant dl, lethal embryos produced by pip females lack ventral and lateral elements; all cells differentiate like the dorsal-most cells of normal embryos (Anderson and Nusslein-Volhard, 1984). Dorsalization is also observed in the pattern of ftz stripes in pip embryos (Carroll et al., 1987). Embryos are not rescued by injection of wild-type cytoplasm; injection of cytoplasm from ventralized embryos of Tl5/+ females, however, partially restores the pip+ embryonic pattern, resulting in differentiation of filzkorper in 25-40% of the treated pip embryos (Anderson et al., 1985).

Summary (Interactive Fly)

heparan sulfate 2-O-sulfotransferase - functions in follicle cells to set up the spatially restricted signal responsible for activating the spatially delimited protease cascade triggering Toll and the establishment of dorsal/ventral polarity during early development.

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\pip for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q86BJ3)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.43

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Gene model reviewed during 5.55

Gene model reviewed during 6.25

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

pip-RA

FBtr0075003

NM_079434

1724

413

pip-RC

FBtr0075004

NM_168793

1397

403

pip-RD

FBtr0075005

NM_176355

1509

418

pip-RF

FBtr0075007

NM_176356

1441

397

pip-RG

FBtr0075008

NM_176354

1614

407

pip-RH

FBtr0075009

NM_176353

1532

405

pip-RI

FBtr0075010

NM_176352

1377

358

pip-RJ

FBtr0075011

NM_176351

1490

401

pip-RK

FBtr0075012

NM_176359

1478

406

pip-RL

FBtr0075013

NM_176358

1848

514

pip-RN

FBtr0333897

NM_176357

1845

513

Additional Transcript Data and Comments

Reported size (kB)

1.7, 1.5 (northern blot)

(Sen et al., 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

pip-PA

FBpp0074770

48.1

413

8.76

NP_524158

AAF49170

pip-PC

FBpp0074771

48.2

403

9.76

NP_730402

AAN11662

pip-PD

FBpp0074772

48.7

418

8.34

NP_788533

AAO41229

pip-PF

FBpp0074774

46.7

397

7.99

NP_788534

AAO41230

pip-PG

FBpp0074775

48.2

407

9.19

NP_788532

AAO41228

pip-PH

FBpp0074776

47.8

405

9.70

NP_788531

AAO41227

pip-PI

FBpp0074777

41.9

358

9.85

NP_788530

AAO41226

pip-PJ

FBpp0074778

47.7

401

10.07

NP_788529

AAO41225

pip-PK

FBpp0074779

47.8

406

8.83

NP_788537

AAO41233

pip-PL

FBpp0074780

60.3

514

7.30

FBpp0306029

60.1

513

7.20

NP_788535

AAO41231

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

403, 392 (aa)

(Sen et al., 1998)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with wbl/windbeutel; the interaction is direct and does not require pip to be folded.

(UniProt, Q86BJ3)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q86BJ3

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\pip using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.51

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 9 -- 10

salivary gland body primordium

Comment: reported as salivary gland anlage

(Fisher et al., 2012)

embryonic stage 11 -- 12

salivary gland body primordium

Comment: reported as salivary gland primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

presumptive embryonic salivary gland

(Sergeev et al., 2001)

larval foregut atrium

(Fisher et al., 2012)

dorsal sensory complex proper primordium

Comment: reported as dorsal/lateral sensory complexes

(Fisher et al., 2012)

embryonic epipharynx

(Fisher et al., 2012)

embryonic foregut

(Fisher et al., 2012)

embryonic/larval posterior spiracle

(Fisher et al., 2012)

embryonic/larval salivary gland

(Fisher et al., 2012)

embryonic/larval salivary gland body

(Fisher et al., 2012)

embryonic stage 16

embryonic/larval salivary gland

(Sen et al., 2000)

oogenesis stage S8 -- S10 & adult stage | female

follicle cell | ventral

(Vreede et al., 2013)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Sen et al., 1998)

embryonic stage 12 -- 17

embryonic/larval pharynx | anterior

(Sen et al., 1998)

embryonic/larval salivary gland

(Sen et al., 1998)

embryonic stage 14 -- 17

gonad | presumptive

(Sen et al., 1998)

adult stage oogenesis stage S9 -- S10

follicle cell | ventral

(Sen et al., 1998)

oogenesis

(Sen et al., 1998)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval salivary gland

(Zhang et al., 2009)

adult stage | female

ovary

(Zhang et al., 2009)

Additional Descriptive Data

Use of isoform-specific oligonucleotides shows that all the major pip isoforms are abundantly expressed in embryos, presumably in salivary glands. Isoforms containing the first 7 alternative exons (after the 3 common amino-terminal exons) are detected in ovaries at a lower level.

(Zhang et al., 2009)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

epitope tag

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis

follicle cell

• Golgi apparatus

(Sen et al., 2000)

immuno-electron microscopy

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis

follicle cell

• Golgi apparatus

(Sen et al., 2000)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in chromosome

inferred from direct assay

(Sen et al., 2000)

located_in Golgi apparatus

inferred from direct assay

(Sen et al., 2000)

located_in nucleus

inferred from direct assay

(Sen et al., 2000)

Expression Deduced from Reporters

Reporter: P{pip-lacZ.5'}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage & oogenesis stage S10

follicle cell | ventral

(Sen et al., 1998)

oogenesis stage S9 -- S10

main body follicle cell

(James et al., 2002)

Reporter: PBac{544.SVS-1}pip^CPTI002126

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain

Comment: low expression throughout adult brain

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\pip in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\pip

View exonic expression by tissue for Dmel\pip

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0003089

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40104/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003089

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003089

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003089

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9614

Flygut - An atlas of the Drosophila adult midgut

FBgn0003089

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\pip

Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 31 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of pip

Transgenic constructs containing regulatory region of pip

Aberrations (Deficiencies and Duplications) ( 27 )

Inferred from experimentation ( 27 )

Gene disrupted in

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Act5C.PU

pip^RNAi.UAS.cYa

lethal | maternal effect (with Df(3L)ED4799)

pip¹

partially lethal - majority die (with Df(3L)pipe^A13)

viable

pip^PL00393

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

pip^GD4622

viable, with Scer\GAL4^Mef2.PR

pip^GD4622

viable, with Scer\GAL4^pnr-MD237

pip^GD4622

viable, with Scer\GAL4^tin.CΔ4

pip^GD4622

Sterility

Allele

fertile

pip^PL00393

Other Phenotypes

Allele

abnormal developmental rate (with Df(3L)pipe^A13)

decreased body size (with Df(3L)pipe^A13)

Phenotype manifest in

Allele

denticle field | maternal effect | somatic clone

pip²

embryo | maternal effect | somatic clone

pip²

embryo | ventral

pip^unspecified

embryonic/first instar larval cuticle

embryonic/first instar larval cuticle | maternal effect

pip¹

embryonic/first instar larval cuticle | maternal effect (with Df(3L)pipe^A13)

pip⁷

embryonic/first instar larval cuticle | maternal effect (with pip²)

pip⁷

embryonic/first instar larval cuticle | maternal effect (with pip⁷)

pip²

embryonic epidermis

pip^unspecified

embryonic epidermis | maternal effect | somatic clone

pip²

embryonic epidermis | ventral

pip¹

presumptive embryonic salivary gland

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (2)

Hsap\UST

6 of 14

Yes

Hsap\HS2ST1

3 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (2)

Rnor\Ust

6 of 14

Yes

Rnor\Hs2st1

3 of 14

Mus musculus (laboratory mouse) (2)

Mmus\Ust

6 of 14

Yes

Mmus\Hs2st1

2 of 14

Xenopus tropicalis (Western clawed frog) (2)

Xtro\hs2st1

2 of 13

Yes

Xtro\ust

2 of 13

Yes

Danio rerio (Zebrafish) (4)

Drer\usta

6 of 14

Yes

Drer\LOC100331876

4 of 14

Yes

Drer\hs2st1b

3 of 14

Drer\hs2st1a

2 of 14

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\hst-2

3 of 14

Yes

Anopheles gambiae (African malaria mosquito) (2)

Agam\AgaP_AGAP006058

11 of 12

Yes

Agam\AgaP_AGAP008166

3 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:pip. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

Hs2st

5 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

UST; uronyl 2-sulfotransferase

6 of 14

610752

HS2ST1; heparan sulfate 2-O-sulfotransferase 1

3 of 14

604844

NEUROFACIOSKELETAL SYNDROME WITH OR WITHOUT RENAL AGENESIS; NFSRA

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

pip - wbl

filter binding

(Barnewitz et al., 2004)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

pip

enhanceable

(Zhang et al., 2009)

pip

enhanceable

Vml

(Zhang et al., 2009)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Cho et al., 2010)

enhanceable

(Cho et al., 2010)

enhanceable

(Cho et al., 2010)

External Data

Subunit Structure (UniProtKB)

Interacts with wbl/windbeutel; the interaction is direct and does not require pip to be folded.

(UniProt, Q86BJ3 )

Linkouts

BioGRID - A database of protein and genetic interactions.

65379

DroID - A comprehensive database of gene and protein interactions.

FBgn0003089

MIST (genetic) - An integrated Molecular Interaction Database

40104

Pathways

Signaling Pathways (FlyBase)

EXTRACELLULAR SPATZLE ACTIVATING PATHWAY CORE COMPONENTS

Metabolic Pathways

FlyBase

External Links

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Dermatan sulfate biosynthesis

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Dermatan sulfate biosynthesis

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-45

Cytogenetic map

76A5-76A6

Sequence location

3L:19,298,623..19,337,609 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

76A5-76A6

Limits computationally determined from genome sequence between P{EP}MESR6^EP3142 and P{lacW}l(3)L3809^L3809

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

76B-76B

(Sen, 1998.10.27)

Experimentally Determined Recombination Data

Location

3-45

(FlyBase, 2016)

3-42.5

(Comeron et al., 2012)

3-47

(Tearle and Nusslein-Volhard, 1987)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (15)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

24732

w¹¹¹⁸; Mi{ET1}pip^MB04958

29054

w¹¹¹⁸; Mi{ET1}pip^MB07037

3110

st¹ pip¹ e¹/TM8, l(3)DTS4¹

34613

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01288}attP2

34178

y¹ w^*; Mi{MIC}pip^MI01309/TM3, Sb¹ Ser¹

50490

y¹ w^*; Mi{MIC}pip^MI08817/TM3, Sb¹ Ser¹

19478

w^*; PBac{GAL4D,EYFP}pip^PL00393 P{FRT(w^hs)}2A P{neoFRT}82B

Kyoto

115472

w¹¹¹⁸; PBac{544.SVS-1}pip^CPTI002126

106851

st¹ pip¹ e¹/TM8, l(3)DTS4¹

NIG-Fly

HMS01288

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01288}attP2

VDRC

v102352

P{KK111537}VIE-260B

v37775

w¹¹¹⁸; P{GD4622}v37775

More stocks available...

Genomic Clones (28)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (75)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

DPiM_RE07829

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0003089

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0003089

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

pip may promote 6-0- and/or N-sulfation of heparan sulfate but is not required for heparan sulfate 2-0-sulfation.

(Park et al., 2008)

pip protein does not show any heparan sulfate-sulfotransferase or chondroitin sulfate-sulfotransferase activity in vitro.

(Xu et al., 2007)

Embryonic extracts derived from a cross between pip mutant mothers and Oregon R males lack the 80 kD ea-Spn27A protein complex seen in wild type embryonic extracts.

(LeMosy, 2006)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

A follicular cell marker system that yields a visible phenotype within the mature egg shell allows direct comparison of a clone and its effect on the dorsal ventral pattern of the embryos. Wild type wbl activity is required only in the cells on the ventral side of the follicular epithelium and is restricted along the A/P axis.

(Nilson and Schupbach, 1998)

Double mutant combinations of pip with ea alleles demonstrate that spatial regulation of ea activity by localized zymogen activation is a key initial event in defining the polarity of the dorsal-ventral embryonic pattern.

(Chasan et al., 1992)

Strong dorsalizing mutants of ndl, snk, gd or pip have wild type axial ratios in pupae.

(Letsou et al., 1991)

Mutations in maternal dorsal class gene pip do not interact with RpII140^wimp.

(Parkhurst and Ish-Horowicz, 1991)

Analysis of mosaic females indicates that the expression of ndl, pip and wbl is required in the somatic tissue, in the follicle cells that surround the oocyte, for the production of embryos with the correct dorsoventral axis.

(Stein et al., 1991)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

CG9614

(Hosono et al., 2015, Yamamoto-Hino et al., 2015, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Häcker et al., 2005)

PIP2

(Križaj et al., 2023, Meyer et al., 2022, Muñoz-Soriano et al., 2018)

Pipe

(Tian and LeMosy, 2008)

l(3)76BDp

(Cooper et al., 2010)

pipe

(Roth, 2023, Gheisari et al., 2020, Martin, 2020, Stathopoulos and Newcomb, 2020, Holmqvist et al., 2012, Technau et al., 2012, Zhang et al., 2009, Hendrix et al., 2008, Hong et al., 2008, Biemar et al., 2005)

pipe-ST2

(Sen, 1998.10.27)

Name Synonyms

Pipe

(Yamamoto-Hino et al., 2015, Lindsay and Wasserman, 2014, Stein et al., 2008, Lippert et al., 2007)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 79 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40104

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q86BJ3

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q86BJ3

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q86BJ3

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0003089

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40104/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003089

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003089

FlyMine - An integrated database for Drosophila genomics

FBgn0003089

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG9614

MARRVEL_MODEL - MARRVEL (model organism gene)

40104

Linkouts

BioGRID - A database of protein and genetic interactions.

65379

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0003089

DroID - A comprehensive database of gene and protein interactions.

FBgn0003089

DRSC - Results frm RNAi screens

FBgn0003089

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003089

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0003089

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9614

Flygut - An atlas of the Drosophila adult midgut

FBgn0003089

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0003089

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC002293

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

40104

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Dermatan sulfate biosynthesis

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Dermatan sulfate biosynthesis

References (255)

The following external sites may use different assemblies or annotations than FlyBase.

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