heparan sulfate 2-O-sulfotransferase - functions in follicle cells to set up the spatially restricted signal responsible for activating the spatially delimited protease cascade triggering Toll and the establishment of dorsal/ventral polarity during early development.
Gene model reviewed during 5.43
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.46
Gene model reviewed during 5.55
Gene model reviewed during 6.25
1.7, 1.5 (northern blot)
None of the polypeptides share 100% sequence identity.
403, 392 (aa)
Interacts directly with windbeutel (wbl).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\pip using the Feature Mapper tool.
Comment: reported as dorsal/lateral sensory complexes
Use of isoform-specific oligonucleotides shows that all the major pip isoforms are abundantly expressed in embryos, presumably in salivary glands. Isoforms containing the first 7 alternative exons (after the 3 common amino-terminal exons) are detected in ovaries at a lower level.
GBrowse - Visual display of RNA-Seq signalsView Dmel\pip in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
pip may promote 6-0- and/or N-sulfation of heparan sulfate but is not required for heparan sulfate 2-0-sulfation.
pip protein does not show any heparan sulfate-sulfotransferase or chondroitin sulfate-sulfotransferase activity in vitro.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
A follicular cell marker system that yields a visible phenotype within the mature egg shell allows direct comparison of a clone and its effect on the dorsal ventral pattern of the embryos. Wild type wbl activity is required only in the cells on the ventral side of the follicular epithelium and is restricted along the A/P axis.
Double mutant combinations of pip with ea alleles demonstrate that spatial regulation of ea activity by localized zymogen activation is a key initial event in defining the polarity of the dorsal-ventral embryonic pattern.
Mutations in maternal dorsal class gene pip do not interact with RpII140wimp.
Analysis of mosaic females indicates that the expression of ndl, pip and wbl is required in the somatic tissue, in the follicle cells that surround the oocyte, for the production of embryos with the correct dorsoventral axis.