Gene model reviewed during 5.39
Gene model reviewed during 5.51
There is only one protein coding transcript and one polypeptide associated with this gene
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\pn using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\pn in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: pn CG3461
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days.
Novel family of predicted phosphoesterases is identified, including the pn gene product.
In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.
To understand the function of pn the nucleotide sequence of a pn cDNA and pn genomic DNA have been determined. Verification that the cDNA encodes a functional pn product is demonstrated by rescue of both the pn eye phenotype and the pn/awdK interaction.
Genetic interactions suggest that pn product in same haematopoetic pathway as awd and hop gene products.
pn shows a lethal interaction with awdK: a small number of larvae reach second instar. A maternal effect can shift the phenocritical period for one larval stage. This effect is temperature sensitive: low temperature, more larvae reach third instar. The perineurium, glia and lymph gland are the main cellular targets of the pn/awdK interaction.
Eye color of newly emerged flies transparent brownish red, becoming brownish purple with age. The eyes of pn1 males have about 25% as much drosopterin (red pigment) as the eyes of wild-type males (FBrf0021089); the concentrations of xanthopterin and sepiapterin (brown pigments) are increased to about 110% of wild-type (FBrf0012680). Control of drosopterin synthesis seems to be related to the activity of the enzyme GTP cyclohydrolase (FBrf0031059). The pn1 eye color is autonomous in larval optic discs transplanted into wild-type hosts (FBrf0003530). Larval Malpighian tube color is normal (FBrf0005752). Standard pn mutants are homo- and hemizygous viable in a wild-type background, but show a lethal interaction with the third chromosome dominant awdK (FBrf0020565; FBrf0024856). Some temperature-sensitive pn mutants ('pnts-e' series), however, are insensitive to the killing action of awdK; one temperature-sensitive mutant (pnts-ek) is insensitive to awdK at permissive temperatures (18oC, 22oC), but sensitive to awdK at restrictive temperatures (25oC, 29oC) (FBrf0024860; FBrf0027926). The TSP for the eye color phenotype occurs during the late pupal stage, while the TSP for the pn component of the pn-awdK interaction begins at the late pupal stage and lasts until eclosion (FBrf0027926). Homo- and hemizygous pn deficiencies and other chromosomal rearrangements have been induced by Ilyina (FBrf0034407).
Analysis of the pn locus suggests that it may encode a cloned 1.4kb cDNA, which encodes a protein with similarity to the catalytic domain of mammalian GTPase-activating proteins.