PPY, PPY 55A
Gene model reviewed during 5.50
Gene model reviewed during 5.55
314 (aa); 36 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\PpY-55A using the Feature Mapper tool.
In situ analysis of male adults shows that expression of PpY-55A transcript is testis-specific.
PpY-55A protein is detected only in extracts from testis on western blots. Immunolocalization studies demonstrate that it is found predominantly in the nuclei of cells distributed along the length of the testis. Staining is weak in the apical region. Colocalization studies with an enhancer trap marker line show that the site of PpY-55A protein expression is the cyst cells.
GBrowse - Visual display of RNA-Seq signalsView Dmel\PpY-55A in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
The PpY-55A gene may have been derived from a related phosphoprotein phosphatase gene by retroposition and subsequent tandem duplication.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
PpY-55A is a testis specific protein phosphatase with biochemical properties distinct from rabbit PP1. Localisation of PpY-55A to somatic cyst cells in testis indicates the protein may be important for differentiation of mature spermatozoa.
Isolated from a Drosophila head cDNA library, using a rabbit serine/threonine-specific protein phosphatase (PP-1) cDNA fragment as a probe.