Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.52
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\sei using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\sei in GBrowse 2
Mapped by B. Ganetzky. Probably maps a short interval (<1 mu) to 2-106, inferred from cytology.
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: sei CG3182
The ppk29 gene regulates neuronal excitability by a protein-independent mechanism, by affecting the level of activity of the sei potassium channel. This regulation occurs via a protein-independent mechanism: the 3'UTR of the ppk29 gene acts as a natural antisense transcript (the two genes are convergently transcribed and the 3'UTRs overlap by 88bp) which regulates the neuronal mRNA levels of sei via the canonical endogenous siRNA pathway. This regulatory interaction is not symmetric (the sei 3'UTR does not regulate ppk29 levels).
The erg polypeptide corresponds to sei, based on sequence analysis and failure of complementation.
From 'tight-seal whole-cell' recordings, using cultured neurons from sei1 embryos, this mutation found to cause sodium current density to be about half-normal; yet, voltage dependence and channel-gating properties were not different from wild type.
Though some of the data mentioned above suggest sei might code for a voltage-sensitive sodium-channel component, this gene could not be co-localized with a known sodium-channel-encoding factor in 2R, which on the basis of clone and sequence plus in situ hybridization, maps within 60D-E.
Paralyzed fairly rapidly at temperatures above 38oC; adults seem to have a fit before becoming immobile, as opposed to immediately ceasing their movements; increasing times of exposure to high temperature leads to progressively longer times necessary for recovery when temperature is lowered.
Wu and Ganetzky.