Sgs-3, Sgs, group IV
AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.43
Gene model reviewed during 5.45
Gene model reviewed during 5.55
There is only one protein coding transcript and one polypeptide associated with this gene
O-glycosylated by Pgnat9 in salivary glands.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Sgs3 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Sgs3 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Sgs3 CG11720
Binding sites for the EcR/usp heterodimer in the Sgs3 upstream region have been identified. Fragments covering the upstream region compete for binding of the ecdysone receptor for the Hsp27 EcRE. Each of the elements 3/I and 3/II is required for the full transcriptional activation of Sgs3, but Sgs3 can be induced to considerable transcriptional activity in the absence of both elements.
fkh protein strongly interacts with the proximal element of Sgs3 and it regulates Sgs3 tissue-specific expression. fkh is expressed in the appropriate tissue and is bound to many loci including the Sgs genes on polytene chromosomes.
Starting from gastrulation the Sgs3 enhancer is maintained in an inactive state by a positioned nucleosomal core particle when Sgs3 is not expressed. This nucleosome is displaced or modified during gene activation. Gebf-I binds to enhancer sequences and mutation of the binding sites reduces Sgs3 expression. Results clearly suggest the direct involvement of Gebf-I in the activity of the enhancer and in the arrangement of an alternative chromatin structure in vivo.
An investigation of the relationship between transcription, puffing and hormone regulation of intermoult puff was analysed using ecd1 mutant embryos: Sgs3 expression is more severely reduced by shifting ecd1 embryos to the restrictive temperature, 30oC, than the reduction seen in Sgs4 embryos.
One of a group of seven genes encoding proteins that are components of the secretion produced by the larval salivary glands during the third instar for the purpose of attaching the larva to the substrate preparative to pupariation.
Six base pairs in the Sgs3 proximal regulatory element that are important in regulating third instar salivary gland-specific expression have been identified using mutated Sgs3-Adh regulatory fusion constructs.
Analysis of deletion constructs of Sgs3 has identified at least three regions important for normal transcription of the gene.
The temporal expression of Sgs3 RNA and the effect of ecdysterone on this expression has been determined.
Expression of Sgs3 as well as Sgs7 and Sgs8 does not take place in the presence of a nonpupariating lethal mutation of the Broad Complex; expression cannot be rescued by the administration of ecdysterone; the mutant does not inhibit formation of intermolt puff 67C, thus dissociating transcription from puff formation.
Initiation of transcription, but not of intermolt-puff formation seems to depend on the presence of suitable levels of ecdysterone in early third instar larvae.
Identification: During a molecular analysis of the 68C puff locus.
Synthesis of the glue proteins begins about 106 h after egg deposition and ceases abruptly within a few minutes after the glue is released 14 h later.
Initiation of transcription of the Sgs genes is coincident with the formation of the intermolt puffs in early to mid third instar.