hls, spnE, homeless, fs(1)hls
DE-H family of RNA-dependent ATPases - possible roles during oocyte development in RNA processing, transport, or stabilization - required to maintain Aub and AGO3 protein levels for piRNA silencing in the Drosophila germline
Please see the JBrowse view of Dmel\spn-E for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.53
4.6 (northern blot)
There is only one protein coding transcript and one polypeptide associated with this gene
1441 (aa); 155 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\spn-E using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
spn-E transcripts are expressed predominantly in ovaries but small amounts are detected in total male and female carcass RNA. In the germarium, expression is limited to the germline cells. Expression decreases through oogenesis stages S4/S5, increases during S8 and peaks in nurse cells at stage S10. No transcript is detected in the oocyte until stage S11 when it is deposited from the nurse cells. spn-E transcripts are uniformly distributed in early embryos.
JBrowse - Visual display of RNA-Seq signals
View Dmel\spn-E in JBrowse3-58
3-57.6
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
spn-E is required for the accumulation of repeat-associated small interfering RNAs (rasiRNAs).
spn-E mutations have a dramatic effect on the distribution of Su(var)205 protein. Instead of the Su(var)205 protein being localised to pericentric heterochromatin and the fourth chromosome it is distributed across the whole of the polytene chromosomes. There is a reduction in the levels of the methyl-K9 form of His3.
spn-E mutants block RNAi activation which normally occurs during egg maturation.
Analysis of mutants of the 5 spindle genes (spn-A, spn-B, mus301, spn-D and spn-E) reveals that the group of genes is required for each of the symmetry-breaking steps that generate polarity during axis formation. spn-E is required for the localisation of fs(1)K10, bcd and osk mRNAs, spn-A. spn-B, mus301 and spn-D play an indirect role in this process.
spn-E gene product is necessary for the correct localisation of several RNAs that are involved in anteroposterior and dorsoventral axis formation during oogenesis.
Source for identity of: spn-E CG3158
Named "homeless" based on oocyte mislocalisation phenotype and RNA localisation phenotype.
