dGATAb, GATAb, l(3)01549, ABF, GATA
transcription factor - zinc finger - GATA family - plays a pivotal role in promoting morphogenesis of the anterior and posterior midgut - necessary for embryonic fat-cell differentiation and other mesodermal derivatives such as hemocytes and lymph gland - regulates hemopoiesis and glucose metabolism
Multiphase exon postulated: exon reading frame differs in alternative transcripts; overlap >20aa.
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.47
Gene model reviewed during 6.32
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\srp using the Feature Mapper tool.
srp transcript expression seen in this study agrees with the results previously documented in FBrf0065324. It is proposed that one of the mesodermal patches of srp expression seen at the blastoderm stage is the hemocyte primordium. After invagination with the ventral furrow, these primordial cells become located laterally to the stomodeum, and later differentiate into prohemocytes which migrate into the head. The cells are subsequently distributed throughout the body and differentiate into mature hemocytes, at which time srp expression is downregulated.
srp transcripts are detected throughout development on northern blots with the highest levels in 6-12hr embryos. In embryos, they are initially detected in four regions of the cellular blastoderm, the anterior and posterior midgut primordia, the primordium of the cephalic mesoderm, and the vitellophages. As gastrulation begins, transcripts are seen in the amnioserosa. During germ band extension, expression diminishes in the midgut but remains strong in the cephalic mesoderm. Later in embryogenesis, srp is expressed in the developing fat body. By stage 15, transcripts are observed exclusively in the fat body.
srp protein is first detected in fat cell precursors at embryonic stage 10, when it is expressed in a cluster of cells in the dorsal mesoderm of parasegment 13, and in segmentally repeated cell clusters in the lateral mesoderm of parasegments 4-9. At embryonic stages 11-12, additional expression is observed in segmentally repeated cell clusters in the ventral mesoderm of parasegments 3-11; these clusters are immediately posterior to the original clusters, and in the equivalent positions of parasegments 10-12.
GBrowse - Visual display of RNA-Seq signalsView Dmel\srp in GBrowse 2
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short spindles when assayed in S2 cells in the presence of Cdc27 dsRNA. This phenotype cannot be observed when the screen is performed without Cdc27 dsRNA.
S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.
dsRNA made from templates generated with primers directed against this gene.
RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
Misexpression of srp in the mesoderm induces the formation of ectopic fat cells and prevents the migration and coalescence of the somatic gonadal precursors. In addition, the differentiation of muscle cells is also affected; few somatic muscle precursors are specified and there is a loss of most somatic muscle fibres and the precursors of the visceral mesoderm and concomitantly the visceral muscle is absent.
srp is required zygotically for the migration of the germ cells through the posterior midgut wall.
There is a balance between fat body and somatic gonadal precursor (SGP) development with tin, wg and en driving cells in the primary clusters towards SGP development and srp driving them towards fat body development.
hh is required for the normal activation of bap and srp in anterior portions of each parasegment, whereas wg is required to suppress bap and srp expression in posterior portions. hh and wg play opposing roles in mesoderm segmentation.
A member of the ush-group of genes which are required for maintenance of the amnioserosa once it has differentiated.
Identification: Identified in a yeast system as a target gene regulated by the bithorax complex proteins.
In the yolk and in the amnioserosa srp function is required for proper gene expression and differentiation.
srp embryos lack the endodermal midgut and instead form additional foregut tissue and duplicated hindgut.
srp is expressed in the ovaries of adult flies where it produces an ovary specific protein isoform. The srp protein binds to a 12bp ovarian follicle cell-specific regulatory element located between the divergently transcribed Yp1 and Yp2. The 12bp element activates both in vivo and in vitro transcription of Yp1 and Yp2.
Identification: Identified as encoding a GATA family transcription factor that binds a conserved sequence element of the larval promoter of the Adh gene. The srp binding sites of the D.melanogaster and D.mulleri Adh larval promoters function as positive control elements.
The expression pattern of srp suggests that it may play a role in the organogenesis of the fat body.