tudor domain protein - serves as 'docking platform' for polar granule assembly - maternal effect gene required for germ cell formation and abdominal segmentation during oogenesis - interacts with Aubergine, a Piwi family protein, in a manner dependent on symmetrically dimethylated arginine residues located at the N-terminal end of Aub
AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.51
None of the polypeptides share 100% sequence identity.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\tud using the Feature Mapper tool.
Northern analysis indicates that the 8 kb tud transcript is present in all stages of development. During oogenesis, tud transcript is first detected in germarium region 2, in one cell within each cyst. This cell occupies a posterior position in later cysts, and is likely to be the oocyte precursor. In stage S1 to S2 egg chambers, tud transcript is detected completely surrounding the oocyte nucleus. From stage S4 to S7 of oogenesis, the tud transcript is detected in the posterior-most region of the oocyte. The amount of tud transcript detected decreases until stage S8, after which no signal is detected in the oocyte. From stage S10 to S14, nurse cells produce large amounts of tud transcript, which is apparently only transiently present in the oocyte, as no tud transcript is detected by in situ localization in late oogenesis, at egg deposition, or through early embryogenesis.
tud protein is detected in the germarium in early oogenesis. By stage S4-S6, tud protein is at the anterior margin of the oocyte, and by stage S6, tud protein is detected at the posterior portion of the oocyte. At stage S10 of oogenesis, tud protein is located in a thin crescent at the posterior of the oocyte. This posterior localization is seen in the unfertilized egg, as well as during the early embryonic stages up to cellularization. EM immunolocalization studies indicate that tud protein is present in the posterior pole plasm, with some of the protein localized to the polar granules, as well as in the mitochondria of cells throughout the early embryo.
GBrowse - Visual display of RNA-Seq signalsView Dmel\tud in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Eight alleles, l(2)57Ce1 to l(2)57Ce8, complement both lethal and grandchildless phenotypes of tud (O'Donnell et al., 1989).
tud is essential for pole cell specification and polar granule formation but is dispensible for somatic posterior patterning and oogenesis.
tud may mediate the transport of mitochondrial rRNAs from mitochondria to polar granules.
tud males produce seminal fluid but no spermatozoa. Lifespan of females mated to Cbβ\DT-A males is greater than those mated to tud males. The entire cost of mating is attributable to receiving male main-cell products, the main cell products have a quantitative effect. The main-cell products are involved in sperm competition, tud males incapacitated 80% of sperm stored by females during a previous mating, males thus increase the proportion of offspring they sire.
Mutations in tud affect two distinct determinative processes in embryogenesis: segmentation in the abdomen and determination of the primordial germ cells. The tud protein is concentrated in the posterior pole cytoplasm where it is found in polar granules and mitochondria. Throughout the rest of the embryo it is associated with cleavage nuclei. Mutations that affect the germ plasm eliminate the posterior localization, and mutations that affect segmentation disrupt the localization around the nuclei.
There is no cost to the female to receive sperm: lifespan, egg production, egg hatchability and remating rate of females intermittently exposed to males that could (tra mutants) or could not (tud mutants) transfer sperm are not significantly different.
tud is not required for the nurse cell synthesis of the posterior signal.
Grandchildless-knirps class of genes.
Identification: Transcription unit identified during molecular analysis of the Pu gene region.
Mutation in tud results in a maternal effect "grandchildless knirps-like" phenotype.
Wieschaus and Nusslein-Volhard.