FlyBase Gene Report: Dmel\ush

General Information

Symbol

Dmel\ush

Species

D. melanogaster

Name

u-shaped

Annotation Symbol

CG2762

Feature Type

protein_coding_gene

FlyBase ID

FBgn0003963

Gene Model Status

Stock Availability

30 publicly available

Gene Summary

u-shaped (ush) encodes a member of the Friend of GATA (FOG) multi-type zinc finger protein family, which bind GATA factors (like those encoded by srp or pnr). These complexes can interface with major signal transduction pathways such as TGFbeta, Hedgehog, JAK/STAT and Insulin/PI3K to regulate diverse biological processes such as embryonic development, heart, blood and neural development and body size. [Date last reviewed: 2018-09-06] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

FOG, dFOG

Key Links

Genomic Location

Cytogenetic map

Sequence location

2L:476,220..540,560 [+]

Recombination map

2-1.5

RefSeq locus

NT_033779 REGION:476220..540560

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (19 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with FLYBASE:srp; FB:FBgn0003507

(Waltzer et al., 2002)

enables RNA polymerase II-specific DNA-binding transcription factor binding

inferred from physical interaction with FLYBASE:pnr; FB:FBgn0003117

(Haenlin et al., 1997)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (16 terms)

Terms Based on Experimental Evidence (14 terms)

CV Term

Evidence

References

involved_in amnioserosa maintenance

inferred from mutant phenotype

(Frank and Rushlow, 1996)

involved_in chaeta development

inferred from genetic interaction with FLYBASE:CtBP; FB:FBgn0020496

(Stern et al., 2009)

involved_in compound eye development

inferred from mutant phenotype

(Fossett et al., 2001)

involved_in germ-band shortening

inferred from mutant phenotype

(Frank and Rushlow, 1996, Goldman-Levi et al., 1996)

involved_in heart development

inferred from mutant phenotype

(Fossett et al., 2001)

involved_in larval lymph gland hemopoiesis

inferred from mutant phenotype

(Gao et al., 2009, Sorrentino et al., 2007)

involved_in lymph gland crystal cell differentiation

inferred from mutant phenotype

(Dragojlovic-Munther and Martinez-Agosto, 2013)

involved_in lymph gland development

inferred from mutant phenotype

(Muratoglu et al., 2006)

involved_in lymph gland plasmatocyte differentiation

inferred from mutant phenotype

(Dragojlovic-Munther and Martinez-Agosto, 2013)

involved_in negative regulation of DNA-binding transcription factor activity

inferred from direct assay

(Haenlin et al., 1997)

involved_in negative regulation of hemocyte differentiation

inferred from mutant phenotype

(Gao et al., 2016)

involved_in negative regulation of transcription by RNA polymerase II

inferred from direct assay

(Cubadda et al., 1997)

inferred from mutant phenotype

(Fromental-Ramain et al., 2010)

involved_in positive regulation of antibacterial peptide biosynthetic process

inferred from mutant phenotype

(Valanne et al., 2010)

involved_in regulation of transcription, DNA-templated

inferred from direct assay

(Cubadda et al., 1997)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in cell differentiation

inferred from biological aspect of ancestor with PANTHER:PTN000311050

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in heart development

inferred from biological aspect of ancestor with PANTHER:PTN000311050

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in negative regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN000311050

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in positive regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN000311050

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(assigned by UniProt )

(Cubadda et al., 1997)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000311050

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

C2H2 ZINC FINGER TRANSCRIPTION FACTORS

Protein Family (UniProt)

Belongs to the FOG (Friend of GATA) family. (Q9VPQ6)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

u-shaped (ush) encodes a member of the Friend of GATA (FOG) multi-type zinc finger protein family, which bind GATA factors (like those encoded by srp or pnr). These complexes can interface with major signal transduction pathways such as TGFbeta, Hedgehog, JAK/STAT and Insulin/PI3K to regulate diverse biological processes such as embryonic development, heart, blood and neural development and body size. [Date last reviewed: 2018-09-06]

Gene Group (FlyBase)

C2H2 ZINC FINGER TRANSCRIPTION FACTORS -: Zinc finger C2H2 transcription factors are sequence-specific DNA binding proteins that regulate transcription. They possess DNA-binding domains that are formed from repeated Cys₂His₂ zinc finger motifs. (Adapted from PMID:1835093, FBrf0220103 and FBrf0155739).

Protein Function (UniProtKB)

Transcription regulator that modulates expression mediated by transcription factors of the GATA family such as pnr and srp. Represses transcription of proneural achaete-scute complex (AS-C), which is usually activated by pnr. Involved in cardiogenesis, blood, and eye development. During hematopoiesis, it is required to restrict the number of crystal cells, probably via its interaction with the isoform SrpNC of srp. Negatively regulates expression of sr. Probably acts by interacting with the GATA-type zinc finger of proteins such as pnr and srp, possibly antagonizing the interaction between the GATA-type zinc finger and some cofactor.

(UniProt, Q9VPQ6)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

ush: u-shaped

Embryonic lethal. No shortening of the germ band. Lateral fusion of anterior and posterior hypoderm.

Summary (Interactive Fly)

multiple zinc finger protein - antagonizes Pnr by physically interacting with the Pnr DNA-binding domain.

Interactive Fly

Gene Model and Products

Number of Transcripts

5

Number of Unique Polypeptides

4

Please see the JBrowse view of Dmel\ush for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VPQ6)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.44

Stop-codon suppression (UGA) postulated; FBrf0216884.

Gene model reviewed during 5.42

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene

gene_with_stop_codon_read_through

(Jungreis et al., 2011)

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0078063

4752

1191

FBtr0310027

4654

1198

FBtr0329895

4482

1212

FBtr0333012

4242

1175

FBtr0333013

6084

1198

Additional Transcript Data and Comments

Reported size (kB)

4.7 (northern blot)

(Cubadda et al., 1997)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

FBpp0077723

124.5

1191

6.35

FBpp0301730

125.4

1198

6.55

FBpp0302929

127.2

1212

6.35

FBpp0305227

122.8

1175

6.50

FBpp0305228

125.4

1198

6.55

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1198 aa isoforms: ush-PB, ush-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

1191 (aa); 123 (kD predicted)

(Cubadda et al., 1997)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with pnr, although weak this interaction is essential. Interacts with the isoform SrpNC of srp. Interacts with CtBP corepressor.

(UniProt, Q9VPQ6)

Domain

The CCHC FOG-type zinc fingers 1, 4 and 5 directly bind to GATA-type zinc fingers. The Tyr residue adjacent to the last Cys of the CCHC FOG-type zinc finger is essential for the interaction with GATA-type zinc fingers.

(UniProt, Q9VPQ6)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ush using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that the Fly Cell Atlas project found the gene expressed in that cell type. Darker colors mean that more cells of that cell type express the gene:

low

high

Colorless tiles indicate that there is no scRNAseq data for the gene in that cell type.

Colored tiles in ribbon indicate that expression data (RNA and/or protein) has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

Colored tiles in the ribbon indicate the average RNA expression level of the gene at the indicated stages:

low

high

as determined by RNA-seq (RPKM) using whole organism samples modENCODE, Brown et al., 2014. For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 8

hemocyte | precursor

(Fossett et al., 2001)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

epithelial cell

• cytoplasm | basal

(Lecuyer et al., 2007)

• cytoplasm | basal

(Lecuyer et al., 2007)

ventral midline of embryo

(Wheeler et al., 2009)

embryonic stage 4 -- 6

dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

(Fisher et al., 2012)

embryonic stage 5

amnioserosa anlage in statu nascendi

(Fossett et al., 2000)

organism | dorsal

(Sandler and Stathopoulos, 2016)

embryonic stage 6

dorsal ectoderm anlage

(Podos et al., 2001)

embryonic stage 6 -- 8

amnioserosa anlage

(Fossett et al., 2000)

embryonic stage 6 -- 12

dorsal ectoderm

(Fossett and Schulz, 2001)

embryonic stage 7 -- 8

amnioserosa anlage

(Fisher et al., 2012)

antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal ectoderm

(Fisher et al., 2012)

(Fisher et al., 2012)

(Fisher et al., 2012)

embryonic stage 8

embryonic hemocyte anlage

(Fossett et al., 2000)

anterior midgut primordium

(Fossett et al., 2000)

posterior midgut primordium

(Fossett et al., 2000)

embryonic stage 9

embryonic hemocyte primordium

(Fossett and Schulz, 2001)

embryonic stage 9 -- 10

amnioserosa primordium

(Fisher et al., 2012)

dorsal ectoderm

(Fisher et al., 2012)

(Fisher et al., 2012)

embryonic stage 11

embryonic hemocyte primordium

(Fossett et al., 2000)

fat body/gonad primordium

(Fossett et al., 2000)

embryonic stage 11 -- 12

(Fisher et al., 2012)

antennal primordium

(Fisher et al., 2012)

dorsal epidermis primordium

(Fisher et al., 2012)

(Fisher et al., 2012)

embryonic stage 11 -- 16

dorsal ectoderm

(Fossett et al., 2000)

embryonic stage 13 -- 16

embryonic dorsal epidermis

(Fisher et al., 2012)

embryonic head epidermis

(Fisher et al., 2012)

embryonic head sensory system

(Fisher et al., 2012)

embryonic/larval garland cell

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

sensory nervous system primordium

(Fisher et al., 2012)

ventral midline of embryo

(Fisher et al., 2012)

embryonic stage 16

ventral midline of embryo

(Fossett et al., 2000)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 9 -- 12

Comment: reference states 4-8 hr AEL

(Cubadda et al., 1997)

third instar larval stage

(Cubadda et al., 1997)

wandering third instar larval stage

imaginal disc | restricted

(Cubadda et al., 1997)

pupal stage & adult stage

(Cubadda et al., 1997)

Additional Descriptive Data

ush transcript is detected in early embryos, in third instar larvae, pupae, and at low levels in adults. In situ hybridization to late third instar larval imaginal discs show ush expression to be restricted to specific domains. These domains include the future dorsal-most region of the thorax, part of the hinge region, and the posterior pleura.

(Cubadda et al., 1997)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 8 -- 10

hemocyte | precursor

(Fossett et al., 2001)

embryonic stage 8 -- 13

(Fossett et al., 2001)

embryonic stage 10 -- 13

embryonic/larval head mesoderm derived hemocyte

(Fossett et al., 2001)

embryonic stage 13 -- 17

embryonic/larval plasmatocyte

(Fossett et al., 2001)

embryonic/larval plasmatocyte of embryonic/larval lymph gland

Comment: primary and secondary lobes of lymph gland

(Fossett et al., 2001)

lymph gland derived hemocyte

(Fossett et al., 2001)

embryonic/larval lymph gland

(Tokusumi et al., 2010)

third instar larval stage

embryonic/larval lymph gland

(Gao et al., 2009, Frandsen et al., 2008, Sorrentino et al., 2007)

(Gao et al., 2009)

embryonic/larval plasmatocyte

(Gao et al., 2009)

embryonic/larval crystal cell

(Gao et al., 2009)

embryonic/larval hemocyte

(Sorrentino et al., 2007)

embryonic/larval pericardial cell

(Sorrentino et al., 2007)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

(Hyun et al., 2009)

Additional Descriptive Data

ush protein is expressed in most lymph gland cells.

(Tokusumi et al., 2010)

The product of ush cDNA transfected into Cos cells localizes to the nucleus. In embryos, ush protein localizes to the nucleus of the cells in which it is expressed.

(Gao et al., 2009, Cubadda et al., 1997)

ush protein is expressed in blood cell lineages. ush is not expressed in crystal cell lineages during later embryonic stages.

(Fossett et al., 2001)

Marker for

embryonic/larval plasmatocyte

(Frandsen et al., 2008)

(Frandsen et al., 2008)

Subcellular Localization

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(assigned by UniProt )

(Cubadda et al., 1997)

Expression Deduced from Reporters

Reporter: P{GawB}NP5133

Stage

Tissue/Position (including subcellular localization)

Reference

dorsal closure stage

amnioserosa | surrounding

(Wada et al., 2007)

Reporter: P{GawB}ush^MD751

Stage

Tissue/Position (including subcellular localization)

Reference

wing disc | restricted

(Usui and Simpson, 2000)

mesothoracic tergum

(Usui and Simpson, 2000)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\ush in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\ush

View exonic expression by tissue for Dmel\ush

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0003963

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33225/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003963

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003963

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003963

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2762

Flygut - An atlas of the Drosophila adult midgut

FBgn0003963

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\ush

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 51 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 39 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of ush

Transgenic constructs containing regulatory region of ush

Aberrations (Deficiencies and Duplications) ( 9 )

Inferred from experimentation ( 9 )

Gene partially disrupted in

Df(2L)TE99(Z)XW88

(Heitzler, 1999.7.20)

In(2L)TE99(Z)XW88-DV1

(Heitzler, 1999.7.20)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal (with Df(2L)BSC107)

ush²

lethal | recessive

lethal - all die before end of first instar larval stage

ush^VE27

lethal - all die before end of P-stage

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

ush^GD1262

lethal - all die during embryonic stage

lethal - all die during embryonic stage | recessive

partially lethal - majority die, with Scer\GAL4^ey.PH

ush^UAS.cFa

partially lethal - majority live

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

ush^GD1262

viable, with Scer\GAL4^Mef2.PR

ush^GD1262

viable, with Scer\GAL4^tin.CΔ4

ush^GD1262

viable, with Scer\GAL4^twi.PB

ush^UAS.cFa

viable (with Df(2L)BSC107)

Other Phenotypes

Allele

abnormal planar polarity, with Scer\GAL4^ey.PH

ush^UAS.cFa

abnormal size | adult stage

ush^UAS.cFa

decreased cell number | third instar larval stage, with Scer\GAL4^Cg.PA

ush^UAS.cFa

decreased cell number | third instar larval stage (with ush^rev24)

ush^VX22

decreased cell number | third instar larval stage (with ush^VX22)

ush^rev24

hyperplasia | dominant | third instar larval stage

ush^VX22

hyperplasia | third instar larval stage (with ush^rev24)

ush^VX22

hyperplasia | third instar larval stage (with ush^VX22)

ush^rev24

increased cell number | dominant | third instar larval stage

ush^VX22

increased cell number | third instar larval stage, with Scer\GAL4^Cg.PA

increased cell number | third instar larval stage (with ush^rev24)

ush^VX22

increased cell number | third instar larval stage (with ush^VX22)

ush^rev24

some die during embryonic stage

some die during embryonic stage | recessive

some die during first instar larval stage

ush^VE27

some die during pharate adult stage

some die during pupal stage, with Scer\GAL4^ey.PH

ush^UAS.cFa

some die during pupal stage, with Scer\GAL4^pnr-MD237

ush^GD1262

visible, with Scer\GAL4^Bx-MS1096

ush^VDRC.cUa

visible, with Scer\GAL4^ey.PH

ush^UAS.cFa

visible, with Scer\GAL4^pnr-MD237

ush^UAS.cCa

visible | adult stage

ush^UAS.cFa

visible | heat sensitive

ush^hs.PC

visible | heat sensitive, with Scer\GAL4^ey.PH

ush^UAS.cFa

visible | male limited, with Scer\GAL4^Bx-MS1096

ush^{UAS.ORF.GW.Tag:HA}

visible | recessive

Phenotype manifest in

Allele

adult external thorax

ush^Q41

adult thorax | dorsal | somatic clone

ush²

antenna, with Scer\GAL4^ey.PH

ush^UAS.cFa

antenna | ectopic, with Scer\GAL4^ey.PH

ush^UAS.cFa

anterior dorsocentral bristle | adult stage, with Scer\GAL4^sca-109-68

anterior notopleural bristle | adult stage, with Scer\GAL4^sca-109-68

anterior postalar bristle | adult stage, with Scer\GAL4^sca-109-68

anterior scutellar bristle | adult stage, with Scer\GAL4^sca-109-68

anterior supraalar bristle | adult stage, with Scer\GAL4^sca-109-68

ush²

cardioblast, with Scer\GAL4^twi.PB

ush^UAS.cFa

cardioblast | ectopic

cephalopharyngeal skeleton

ush²

ush²

direct flight muscle cell

ush¹⁵¹³

direct flight muscle cell (with Df(2L)ush^rev18)

ush^rev5

ush²

dorsal medial indirect flight muscle cell

ush¹⁵¹³

dorsocentral bristle

dorsocentral bristle, with Scer\GAL4^pnr-MD237

ush^UAS.cCa

dorsocentral bristle & axon

ush^rev24

dorsocentral bristle | cell non-autonomous | somatic clone

dorsocentral bristle | heat sensitive

ush^hs.PC

dorsocentral bristle | precursor, with Scer\GAL4^pnr-MD237

ush^UAS.cCa

egg chorion | drug conditional, with Tn10\tetR^rtTA.Act5C

ush^3-38

embryo | dorsal closure stage

ush²

embryonic/larval crystal cell, with Scer\GAL4^lz-gal4

ush^UAS.cFa

embryonic/larval crystal cell, with Scer\GAL4^twi.PB

ush^UAS.cFa

embryonic/larval crystal cell | ectopic

ush¹

embryonic/larval crystal cell | embryonic stage, with Scer\GAL4^lz-gal4

ush^UAS.cCa

embryonic/larval crystal cell | larval stage

ush^VX22

embryonic/larval crystal cell | third instar larval stage

ush^VX22

embryonic/larval lymph gland, with Scer\GAL4^Mef2.PR

ush^UAS.cFa

embryonic/larval lymph gland, with Scer\GAL4^twi.PB

ush^UAS.cFa

embryonic/larval lymph gland (with ush^rev24)

ush^VX22

embryonic/larval lymph gland (with ush^VX22)

ush^rev24

embryonic/larval lymph gland | larval stage

ush^VX22

embryonic/larval lymph gland | second instar larval stage, with Scer\GAL4^Cg.PA

ush^302-1191.UAS

embryonic/larval lymph gland | second instar larval stage (with ush^rev24)

ush^VX22

embryonic/larval lymph gland | second instar larval stage (with ush^VX22)

ush^rev24

embryonic/larval lymph gland | third instar larval stage

ush^VX22

embryonic/larval lymph gland | third instar larval stage (with ush^rev24)

ush^VX22

embryonic/larval lymph gland | third instar larval stage (with ush^VX22)

ush^rev24

embryonic/larval plasmatocyte | third instar larval stage

ush^VX22

embryonic/larval plasmatocyte | third instar larval stage, with Scer\GAL4^Cg.PA

ush^UAS.cFa

embryonic/larval plasmatocyte | third instar larval stage (with ush^rev24)

ush^VX22

embryonic/larval plasmatocyte | third instar larval stage (with ush^VX22)

ush^rev24

ush²

epidermis | anterior

ush^unspecified

epidermis | posterior

ush^unspecified

eye, with Scer\GAL4^ey.PH

ush^UAS.cFa

eye disc | heat sensitive, with Scer\GAL4^ey.PH

ush^UAS.cFa

fat body | somatic clone, with Scer\GAL4^Act.PU

ush^UAS.cCa

ush²

heart primordium, with Scer\GAL4^twi.PB

ush^UAS.cFa

hemocyte | larval stage

ush^VX22

hemocyte | third instar larval stage, with Scer\GAL4^Cg.PA

hemocyte | third instar larval stage (with ush^rev24)

ush^VX22

hemocyte | third instar larval stage (with ush^VX22)

ush^rev24

lamellocyte (with ush^rev24)

ush^VX22

lamellocyte (with ush^VX22)

ush^rev24

lamellocyte | larval stage

ush^VX22

lamellocyte | third instar larval stage

ush^VX22

lamellocyte | third instar larval stage, with Scer\GAL4^Cg.PA

lamellocyte | third instar larval stage (with ush^rev24)

ush^VX22

lamellocyte | third instar larval stage (with ush^VX22)

ush^rev24

ush¹

mitotic domain 1 | extended germ band stage

ommatidium, with Scer\GAL4^ey.PH

ush^UAS.cFa

pericardial cell | ectopic

posterior dorsocentral bristle | adult stage, with Scer\GAL4^sca-109-68

posterior notopleural bristle | adult stage, with Scer\GAL4^sca-109-68

posterior scutellar bristle | adult stage, with Scer\GAL4^sca-109-68

ush^UAS.cSa

posterior signaling center of lymph gland primary lobe, with Scer\GAL4^{kn-col85-GAL4}

ush^UAS.cFa

posterior supraalar bristle | adult stage, with Scer\GAL4^sca-109-68

postvertical bristle

presutural bristle | adult stage, with Scer\GAL4^sca-109-68

ush^UAS.cSa

scutellar bristle

scutellum | somatic clone

vertical bridge

ush²

wing, with Scer\GAL4^Bx-MS1096

ush^VDRC.cUa

wing | male limited, with Scer\GAL4^Bx-MS1096

ush^{UAS.ORF.GW.Tag:HA}

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

6 of 15

Yes

Yes

5 of 15

No

Yes

1 of 15

No

No

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

6 of 15

Yes

Yes

1

5 of 15

No

Yes

2

1 of 15

No

No

Rattus norvegicus (Norway rat) (2)

5 of 13

Yes

Yes

1 of 13

No

Yes

Xenopus tropicalis (Western clawed frog) (3)

4 of 12

Yes

Yes

4 of 12

Yes

Yes

1 of 12

No

Yes

Danio rerio (Zebrafish) (3)

5 of 15

Yes

Yes

4 of 15

No

Yes

4 of 15

No

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

2 of 15

Yes

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 3 )

Human Ortholog

Disease

Evidence

References

ZFPM2; zinc finger protein, FOG family member 2

model of tetralogy of Fallot

IEA

(FlyBase, 2019-)

model of congenital diaphragmatic hernia

IEA

(FlyBase, 2019-)

model of 46,XY sex reversal 9

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

exacerbates hematologic cancer

modeled by hop^Tum

(Bausek and Zeidler, 2014)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ZFPM1; zinc finger protein, FOG family member 1

6 of 15

ZFPM2; zinc finger protein, FOG family member 2

5 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

RNA-RNA

Physical Interaction

Assay

References

luminiscence technology, western blot, quantitative reverse transcription pcr

(Lee and Hyun, 2014, Hyun et al., 2009)

protein-protein

Physical Interaction

Assay

References

experimental knowledge based

(Guruharsha et al., 2011)

anti tag coimmunoprecipitation, western blot

(Lenz et al., 2021)

anti tag coimmunoprecipitation, anti tag western blot, pull down, autoradiography

(Biryukova and Heitzler, 2008)

ush - MTA1-like

anti tag coimmunoprecipitation, western blot

(Lenz et al., 2021)

anti tag coimmunoprecipitation, western blot

(Lenz et al., 2021)

bimolecular fluorescence complementation, fluorescence microscopy

(Bischof et al., 2018)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

anti tag coimmunoprecipitation, western blot

(Lenz et al., 2021)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Fossett et al., 2000)

suppressible

(Sorrentino et al., 2007)

enhanceable

(Fossett et al., 2003, Goldman-Levi et al., 1996)

enhanceable

(Goldman-Levi et al., 1996)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Biryukova and Heitzler, 2008)

suppressible

(Stern et al., 2009)

enhanceable

(Bausek and Zeidler, 2014)

suppressible

(Sorrentino et al., 2007)

enhanceable

(Fossett et al., 2000)

suppressible

(Singh et al., 2005)

suppressible

(Hyun et al., 2009)

enhanceable

(Goldman-Levi et al., 1996)

enhanceable

(Cubadda et al., 1997)

suppressible

(Fossett et al., 2003)

enhanceable

(Goldman-Levi et al., 1996)

enhanceable

(Goldman-Levi et al., 1996)

External Data

Subunit Structure (UniProtKB)

Interacts with pnr, although weak this interaction is essential. Interacts with the isoform SrpNC of srp. Interacts with CtBP corepressor.

(UniProt, Q9VPQ6 )

Linkouts

BioGRID - A database of protein and genetic interactions.

59481

DroID - A comprehensive database of gene and protein interactions.

FBgn0003963

MIST (genetic) - An integrated Molecular Interaction Database

ush

MIST (protein-protein) - An integrated Molecular Interaction Database

ush

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0003963

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

2L

Recombination map

2-1.5

Cytogenetic map

Sequence location

2L:476,220..540,560 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

21E1-21E2

Limits computationally determined from genome sequence between P{lacW}l(2)k09610^k09610&P{lacW}S^k09538a and P{PZ}l(2)10685¹⁰⁶⁸⁵&P{lacW}Tango14^k00619

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-1.5

(FlyBase, 2016)

2-0.1

(Comeron et al., 2012)

2-0

(Tearle and Nusslein-Volhard, 1987)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (30)

Aberrations including deletions of this gene Aberrations including duplications of this gene

23467

w¹¹¹⁸; Mi{ET1}ush^MB02155

18988

w¹¹¹⁸; PBac{WH}ush^f06555/CyO

62667

w¹¹¹⁸; PBac{IT.GAL4}ush^0144-G4

63750

w¹¹¹⁸; PBac{IT.GAL4}ush^0697-G4

26662

y¹ w^*; P{GawB}ush^MD751

93098

y¹ w^*; PBac{ush-GFP.FPTB}VK00031

F001549

M{UAS-ush.ORF}ZH-86Fb

F004833

M{UAS-ush.ORF-VN}ZH-86Fb

F001800

M{UAS-ush.ORF.3xHA.GW}ZH-86Fb

106086

In(2L)Tg, ush^Tg dpp^Tg/SM5

v104102

P{KK104016}VIE-260B

v5712

w¹¹¹⁸; P{GD1262}v5712

More stocks available...

Genomic Clones (37)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (29)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD12631

Other clones

SD10668

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0003963

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0003963

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

33225

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Cubadda et al., 1997)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: ush CG2762

(Guan, 1999.11)

Source for database merge of

Additional comments

Other Comments

S2-derived S2-NP cells are treated with dsRNA made from templates generated with primers directed against ush to assess the role of this gene in Stat92E tyrosine phosphorylation.

(Baeg et al., 2005)

ush is required for os-induced Stat92E phosphorylation.

(Baeg et al., 2005)

ush is a negative regulator of hemocoel, heart and eye development.

(Fossett et al., 2001)

Identified as a gene with significant level of mRNA cycling as assessed by expression analysis using high density oligonucleotide arrays with probe generated from adult heads harvested over six time points over the course of a day.

(McDonald and Rosbash, 2001)

ush has a critical role in several diverse processes of mesoderm differentiation and heart formation.

(Fossett et al., 2000)

The pnr/ush complex serves as a repressor and a transcriptional activator, respectively for wg and ush expression.

(Sato and Saigo, 2000)

The dpp gradient may regulate notal wg expression through induction of pnr and ush.

(Tomoyasu et al., 2000)

pnr and ush are main effectors of the regulation of wg expression in the presumptive notum.

(Garcia-Garcia et al., 1999)

pnr and ush provide positional information for the patterning of the dorsocentral mechanosensory bristle cluster.

(Garcia-Garcia et al., 1999)

ush encodes a zinc-finger protein that regulates the proneural genes ac and sc during bristle formation.

(Cubadda et al., 1997)

A member of the ush-group of genes which are required for maintenance of the amnioserosa once it has differentiated.

(Frank and Rushlow, 1996)

Mutants affect amnioserosa differentiation as well as germ band retraction.

(Frank and Rushlow, 1995)

Mutations in zygotic gene ush do not interact with RpII140^wimp.

(Parkhurst and Ish-Horowicz, 1991)

Zygotically active locus involved in the terminal developmental program in the embryo.

(Strecker et al., 1991)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 65 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

33225

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VPQ6

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VPQ6

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VPQ6

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0003963

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0003963

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33225/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003963

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003963

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003963

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2762

Flygut - An atlas of the Drosophila adult midgut

FBgn0003963

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0003963

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

33225

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC013689

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG2762

MARRVEL_MODEL - MARRVEL (model organism gene)

33225

modMine - A data warehouse for the modENCODE project

FBgn0003963

PDB - An information portal to biological macromolecular structures

Linkouts

BioGRID - A database of protein and genetic interactions.

59481

DroID - A comprehensive database of gene and protein interactions.

FBgn0003963

DRSC - Results frm RNAi screens

FBgn0003963

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0003963

FlyMine - An integrated database for Drosophila genomics

FBgn0003963

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

ush

MIST (protein-protein) - An integrated Molecular Interaction Database

ush

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

CG2762

(Schertel et al., 2013, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Khokhar et al., 2008)

FOG

(Vlisidou and Wood, 2015, Fossett and Schulz, 2001)

USH

(Lehmann, 2018, Lee et al., 2015, Hyun, 2013)

Ush

(Lan et al., 2020, Yu et al., 2018, Van Bortle et al., 2015, Gao et al., 2014, Honti et al., 2014, Lee and Hyun, 2014, Wang et al., 2014, Amoyel and Bach, 2012, Chlon and Crispino, 2012, Tokusumi et al., 2010, Tokusumi et al., 2009, Frandsen et al., 2008, Gesellchen et al., 2005, Fossett et al., 2001)

ZFPM2

(Gieger et al., 2011)

dFOG

(Fossett, 2013, Liew et al., 2005)

l(2)19

u-sh

(Haenlin et al., 1995)

ush

(Dufourt et al., 2021, Hoppe and Ashe, 2021, Moussalem et al., 2021, Winbush and Singh, 2021, Creed et al., 2020, Hoppe et al., 2020, Lepesant et al., 2020, Luo et al., 2020, Overton et al., 2020, Soluri et al., 2020, Banerjee et al., 2019, Michalak et al., 2019, Baldeosingh et al., 2018, Bischof et al., 2018, Rajpurohit et al., 2018, Karaiskos et al., 2017, Liu et al., 2017, Transgenic RNAi Project members, 2017-, Brookheart and Duncan, 2016, Clandinin and Owens, 2016-, Gao et al., 2016, Gomez et al., 2016, Sandler and Stathopoulos, 2016, Sandler and Stathopoulos, 2016, Duff et al., 2015, Morán et al., 2015, Newton et al., 2015, Sawala et al., 2015, Schertel et al., 2015, Vedanayagam and Garrigan, 2015, Vlisidou and Wood, 2015, Winstanley et al., 2015, Bausek and Zeidler, 2014, Bischof, 2014.10.20, Gonzalez et al., 2014, Kim and Choe, 2014, Salazar-Jaramillo et al., 2014, Valanne, 2014, de Celis et al., 2013.9.11, Saunders et al., 2013, Schertel et al., 2013, Amoyel and Bach, 2012, Belozerov et al., 2012, Garcia et al., 2012, Hainaut et al., 2012, Hironaka et al., 2012, Holmqvist et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Jin et al., 2012, Kennell et al., 2012, Lada et al., 2012, Gao et al., 2011, Gieger et al., 2011, Jungreis et al., 2011, Avet-Rochex et al., 2010, Campos et al., 2010, Fromental-Ramain et al., 2010, Heitzler, 2010.5.12, Pauli et al., 2010, Simone and DiNardo, 2010, Sorrentino, 2010, Stofanko et al., 2010, Boettiger and Levine, 2009, Gao et al., 2009, Hyun et al., 2009, Stern et al., 2009, Wheeler et al., 2009, Biryukova and Heitzler, 2008, Khokhar et al., 2008, Kwon et al., 2008, Kwong et al., 2008, Lee et al., 2008, Miles et al., 2008, Wang et al., 2008, Zhou et al., 2008, Baeg et al., 2007, de Navascués and Modolell, 2007, Fernández et al., 2007, Lecuyer et al., 2007, Letizia et al., 2007, Liang et al., 2007, Sorrentino et al., 2007, Tokusumi et al., 2007, Wada et al., 2007, Mizutani et al., 2006, Sandmann et al., 2006, Sellin et al., 2006, Simpson et al., 2006, Scuderi and Letsou, 2005, Stathopoulos and Levine, 2005)

ushaped

(Fossett and Schulz, 2001)

Name Synonyms

Drosophila Friend of GATA

(Muratoglu et al., 2006)

Friend of GATA

(Sorrentino et al., 2007)

U-shaped

(Ramond et al., 2020, Yu et al., 2018, Gao et al., 2016, Kanoh et al., 2015, Lucas et al., 2015, Gao et al., 2014, Wang et al., 2014, Lada et al., 2012, Fromental-Ramain et al., 2010, Tokusumi et al., 2010, Stern et al., 2009, Biryukova and Heitzler, 2008, Frandsen et al., 2008, Letizia et al., 2007, Muratoglu et al., 2007, Sorrentino et al., 2007, Tokusumi et al., 2007, Gesellchen et al., 2005)

ushaped

(Khokhar et al., 2008)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

References (284)