FB2021_02, released April 13, 2021

Gene: Dmel\ush

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General Information
Symbol
Dmel\ush
Species
D. melanogaster
Name
u-shaped
Annotation Symbol
CG2762
Feature Type
protein_coding_gene
FlyBase ID
FBgn0003963
Gene Model Status
Current
Stock Availability
29 publicly available
Gene Summary
u-shaped (ush) encodes a member of the Friend of GATA (FOG) multi-type zinc finger protein family, which bind GATA factors (like those encoded by srp or pnr). These complexes can interface with major signal transduction pathways such as TGFbeta, Hedgehog, JAK/STAT and Insulin/PI3K to regulate diverse biological processes such as embryonic development, heart, blood and neural development and body size. [Date last reviewed: 2018-09-06] (FlyBase Gene Snapshot)
All Summaries
Also Known As

FOG, dFOG

Key Links
Genomic Location
Cytogenetic map
21E1-21E2
Sequence location
2L:476,220..540,560 [+]
Recombination map
2-1.5
RefSeq locus
NT_033779 REGION:476220..540560
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (21 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
enables protein binding
inferred from physical interaction with FLYBASE:srp; FB:FBgn0003507
(Waltzer et al., 2002)
enables RNA polymerase II activating transcription factor binding
inferred from physical interaction with FLYBASE:pnr; FB:FBgn0003117
(Haenlin et al., 1997)
enables transcription factor binding
inferred from mutant phenotype
(assigned by UniProt )
(Cubadda et al., 1997)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables RNA polymerase II transcription factor binding
inferred from biological aspect of ancestor with PANTHER:PTN000311050
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (16 terms)
Terms Based on Experimental Evidence (14 terms)
CV Term
Evidence
References
involved_in amnioserosa maintenance
inferred from mutant phenotype
(Frank and Rushlow, 1996)
involved_in chaeta development
inferred from genetic interaction with FLYBASE:CtBP; FB:FBgn0020496
(Stern et al., 2009)
involved_in compound eye development
inferred from mutant phenotype
(Fossett et al., 2001)
involved_in germ-band shortening
inferred from mutant phenotype
(Frank and Rushlow, 1996, Goldman-Levi et al., 1996)
involved_in heart development
inferred from mutant phenotype
(Fossett et al., 2001)
involved_in larval lymph gland hemopoiesis
inferred from mutant phenotype
(Gao et al., 2009, Sorrentino et al., 2007)
involved_in lymph gland crystal cell differentiation
inferred from mutant phenotype
(Dragojlovic-Munther and Martinez-Agosto, 2013)
involved_in lymph gland development
inferred from mutant phenotype
(Muratoglu et al., 2006)
involved_in lymph gland plasmatocyte differentiation
inferred from mutant phenotype
(Dragojlovic-Munther and Martinez-Agosto, 2013)
involved_in negative regulation of DNA-binding transcription factor activity
inferred from direct assay
(Haenlin et al., 1997)
involved_in negative regulation of hemocyte differentiation
inferred from mutant phenotype
(Gao et al., 2016)
involved_in negative regulation of transcription by RNA polymerase II
inferred from direct assay
(Cubadda et al., 1997)
inferred from mutant phenotype
(Fromental-Ramain et al., 2010)
involved_in positive regulation of antibacterial peptide biosynthetic process
inferred from mutant phenotype
(Valanne et al., 2010)
involved_in regulation of transcription, DNA-templated
inferred from direct assay
(Cubadda et al., 1997)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
involved_in cell differentiation
inferred from biological aspect of ancestor with PANTHER:PTN000311050
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in heart development
inferred from biological aspect of ancestor with PANTHER:PTN000311050
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in negative regulation of transcription by RNA polymerase II
inferred from biological aspect of ancestor with PANTHER:PTN000311050
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in positive regulation of transcription by RNA polymerase II
inferred from biological aspect of ancestor with PANTHER:PTN000311050
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in nucleus
inferred from direct assay
(assigned by UniProt )
(Cubadda et al., 1997)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
located_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000311050
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the FOG (Friend of GATA) family. (Q9VPQ6)
Protein Signatures (InterPro)
Summaries
Gene Snapshot
u-shaped (ush) encodes a member of the Friend of GATA (FOG) multi-type zinc finger protein family, which bind GATA factors (like those encoded by srp or pnr). These complexes can interface with major signal transduction pathways such as TGFbeta, Hedgehog, JAK/STAT and Insulin/PI3K to regulate diverse biological processes such as embryonic development, heart, blood and neural development and body size. [Date last reviewed: 2018-09-06]
Gene Group (FlyBase)
C2H2 ZINC FINGER TRANSCRIPTION FACTORS -
Zinc finger C2H2 transcription factors are sequence-specific DNA binding proteins that regulate transcription. They possess DNA-binding domains that are formed from repeated Cys2His2 zinc finger motifs. (Adapted from PMID:1835093, FBrf0220103 and FBrf0155739).
Protein Function (UniProtKB)
Transcription regulator that modulates expression mediated by transcription factors of the GATA family such as pnr and srp. Represses transcription of proneural achaete-scute complex (AS-C), which is usually activated by pnr. Involved in cardiogenesis, blood, and eye development. During hematopoiesis, it is required to restrict the number of crystal cells, probably via its interaction with the isoform SrpNC of srp. Negatively regulates expression of sr. Probably acts by interacting with the GATA-type zinc finger of proteins such as pnr and srp, possibly antagonizing the interaction between the GATA-type zinc finger and some cofactor.
(UniProt, Q9VPQ6)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
ush: u-shaped
Embryonic lethal. No shortening of the germ band. Lateral fusion of anterior and posterior hypoderm.
Summary (Interactive Fly)

multiple zinc finger protein - antagonizes Pnr by physically interacting with the Pnr DNA-binding domain.

Gene Model and Products
Number of Transcripts
5
Number of Unique Polypeptides
4

Please see the JBrowse view of Dmel\ush for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.44

Stop-codon suppression (UGA) postulated; FBrf0216884.

Gene model reviewed during 5.42

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene
gene_with_stop_codon_read_through
(Jungreis et al., 2011)
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
ush-RA
FBtr0078063
NM_057432
4752
1191
ush-RB
FBtr0310027
NM_001272899
4654
1198
ush-RC
FBtr0329895
NM_001272900
4482
1212
ush-RD
FBtr0333012
NM_001272901
4242
1175
ush-RE
FBtr0333013
NM_001272902
6084
1198
Additional Transcript Data and Comments
Reported size (kB)

4.7 (northern blot)

(Cubadda et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
ush-PA
FBpp0077723
124.5
1191
6.35
NP_476780
AAF51488
ush-PB
FBpp0301730
125.4
1198
6.55
NP_001259828
AGB92365
ush-PC
FBpp0302929
127.2
1212
6.35
NP_001259829
AGB92366
ush-PD
FBpp0305227
122.8
1175
6.50
NP_001259830
AGB92367
ush-PE
FBpp0305228
125.4
1198
6.55
NP_001259831
AGB92368
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1198 aa isoforms: ush-PB, ush-PE
Additional Polypeptide Data and Comments
Reported size (kDa)

1191 (aa); 123 (kD predicted)

(Cubadda et al., 1997)
Comments
External Data
Subunit Structure (UniProtKB)

Interacts with pnr, although weak this interaction is essential. Interacts with the isoform SrpNC of srp. Interacts with CtBP corepressor.

(UniProt, Q9VPQ6)
Domain

The CCHC FOG-type zinc fingers 1, 4 and 5 directly bind to GATA-type zinc fingers. The Tyr residue adjacent to the last Cys of the CCHC FOG-type zinc finger is essential for the interaction with GATA-type zinc fingers.

(UniProt, Q9VPQ6)
Crossreferences
InterPro - A database of protein families, domains and functional sites
PDB - An information portal to biological macromolecular structures
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ush using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 8
hemocyte | precursor
(Fossett et al., 2001)
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
epithelial cell
• cytoplasm | basal
(Lecuyer et al., 2007)
epithelium
• cytoplasm | basal
(Lecuyer et al., 2007)
ventral midline of embryo
(Wheeler et al., 2009)
embryonic stage 4 -- 6
dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
yolk nucleus
(Fisher et al., 2012)
embryonic stage 5
amnioserosa anlage in statu nascendi
(Fossett et al., 2000)
organism | dorsal
(Sandler and Stathopoulos, 2016)
embryonic stage 6
dorsal ectoderm anlage
(Podos et al., 2001)
embryonic stage 6 -- 8
amnioserosa anlage
(Fossett et al., 2000)
embryonic stage 6 -- 12
dorsal ectoderm
(Fossett and Schulz, 2001)
embryonic stage 7 -- 8
amnioserosa anlage
(Fisher et al., 2012)
antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
dorsal ectoderm
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
yolk nucleus
(Fisher et al., 2012)
embryonic stage 8
embryonic hemocyte anlage
(Fossett et al., 2000)
anterior midgut primordium
(Fossett et al., 2000)
posterior midgut primordium
(Fossett et al., 2000)
embryonic stage 9
embryonic hemocyte primordium
(Fossett and Schulz, 2001)
embryonic stage 9 -- 10
amnioserosa primordium
(Fisher et al., 2012)
dorsal ectoderm
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
embryonic stage 11
embryonic hemocyte primordium
(Fossett et al., 2000)
fat body/gonad primordium
(Fossett et al., 2000)
embryonic stage 11 -- 12
amnioserosa
(Fisher et al., 2012)
antennal primordium
(Fisher et al., 2012)
dorsal epidermis primordium
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
embryonic stage 11 -- 16
dorsal ectoderm
(Fossett et al., 2000)
embryonic stage 13 -- 16
embryonic dorsal epidermis
(Fisher et al., 2012)
embryonic head epidermis
(Fisher et al., 2012)
embryonic head sensory system
(Fisher et al., 2012)
embryonic/larval garland cell
(Fisher et al., 2012)
embryonic/larval midgut
(Fisher et al., 2012)
sensory nervous system primordium
(Fisher et al., 2012)
ventral midline of embryo
(Fisher et al., 2012)
embryonic stage 16
ventral midline of embryo
(Fossett et al., 2000)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 9 -- 12

Comment: reference states 4-8 hr AEL

(Cubadda et al., 1997)
third instar larval stage
(Cubadda et al., 1997)
wandering third instar larval stage
imaginal disc | restricted
(Cubadda et al., 1997)
pupal stage & adult stage
(Cubadda et al., 1997)
Additional Descriptive Data

ush transcript is detected in early embryos, in third instar larvae, pupae, and at low levels in adults. In situ hybridization to late third instar larval imaginal discs show ush expression to be restricted to specific domains. These domains include the future dorsal-most region of the thorax, part of the hinge region, and the posterior pleura.

(Cubadda et al., 1997)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 8 -- 10
hemocyte | precursor
(Fossett et al., 2001)
embryonic stage 8 -- 13
hemocyte
(Fossett et al., 2001)
embryonic stage 10 -- 13
head mesoderm derived embryonic hemocyte
(Fossett et al., 2001)
embryonic stage 13 -- 17
plasmatocyte
(Fossett et al., 2001)
larval stage
plasmatocyte of embryonic/larval lymph gland

Comment: primary and secondary lobes of lymph gland

(Fossett et al., 2001)
lymph gland derived hemocyte
(Fossett et al., 2001)
embryonic/larval lymph gland
(Tokusumi et al., 2010)
third instar larval stage
embryonic/larval lymph gland
(Gao et al., 2009, Frandsen et al., 2008, Sorrentino et al., 2007)
prohemocyte
(Gao et al., 2009)
plasmatocyte
(Gao et al., 2009)
crystal cell
(Gao et al., 2009)
embryonic/larval hemocyte
(Sorrentino et al., 2007)
embryonic/larval pericardial cell
(Sorrentino et al., 2007)
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
fat body
(Hyun et al., 2009)
Additional Descriptive Data

ush protein is expressed in most lymph gland cells.

(Tokusumi et al., 2010)

The product of ush cDNA transfected into Cos cells localizes to the nucleus. In embryos, ush protein localizes to the nucleus of the cells in which it is expressed.

(Gao et al., 2009, Cubadda et al., 1997)

ush protein is expressed in blood cell lineages. ush is not expressed in crystal cell lineages during later embryonic stages.

(Fossett et al., 2001)
Marker for
 
plasmatocyte
(Frandsen et al., 2008)
prohemocyte
(Frandsen et al., 2008)
Subcellular Localization
CV Term
Evidence
References
located_in nucleus
inferred from direct assay
(assigned by UniProt )
(Cubadda et al., 1997)
Expression Deduced from Reporters
Reporter: P{GawB}NP5133
Stage
Tissue/Position (including subcellular localization)
Reference
dorsal closure stage
amnioserosa | surrounding
(Wada et al., 2007)
Reporter: P{GawB}ushMD751
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
wing disc | restricted
(Usui and Simpson, 2000)
pupal stage
mesothoracic tergum
(Usui and Simpson, 2000)
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\ush in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 48 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 38 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of ush
Transgenic constructs containing regulatory region of ush
Aberrations (Deficiencies and Duplications) ( 9 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal (with Df(2L)BSC107)
lethal | recessive
lethal - all die before end of first instar larval stage
lethal - all die before end of P-stage
lethal - all die before end of pupal stage, with Scer\GAL4pnr-MD237
lethal - all die during embryonic stage
lethal - all die during embryonic stage | recessive
partially lethal - majority die, with Scer\GAL4ey.PH
partially lethal - majority live
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4tin.CΔ4
viable, with Scer\GAL4twi.PB
viable (with Df(2L)BSC107)
Other Phenotypes
Allele
abnormal planar polarity, with Scer\GAL4ey.PH
abnormal size | adult stage
decreased cell number | third instar larval stage, with Scer\GAL4Cg.PA
decreased cell number | third instar larval stage (with ushrev24)
decreased cell number | third instar larval stage (with ushVX22)
hyperplasia | dominant | third instar larval stage
hyperplasia | third instar larval stage (with ushrev24)
hyperplasia | third instar larval stage (with ushVX22)
increased cell number | dominant | third instar larval stage
increased cell number | third instar larval stage, with Scer\GAL4Cg.PA
increased cell number | third instar larval stage (with ushrev24)
increased cell number | third instar larval stage (with ushVX22)
some die during embryonic stage
some die during embryonic stage | recessive
some die during first instar larval stage
some die during pharate adult stage
some die during pupal stage, with Scer\GAL4ey.PH
some die during pupal stage, with Scer\GAL4pnr-MD237
visible, with Scer\GAL4Bx-MS1096
visible, with Scer\GAL4ey.PH
visible, with Scer\GAL4pnr-MD237
visible | adult stage
visible | heat sensitive
visible | heat sensitive, with Scer\GAL4ey.PH
visible | male limited, with Scer\GAL4Bx-MS1096
visible | recessive
wild-type
Phenotype manifest in
Allele
adult external thorax
adult thorax
adult thorax | dorsal | somatic clone
amnioserosa
antenna, with Scer\GAL4ey.PH
antenna | ectopic, with Scer\GAL4ey.PH
anterior dorsocentral bristle | adult stage, with Scer\GAL4sca-109-68
anterior notopleural bristle | adult stage, with Scer\GAL4sca-109-68
anterior postalar bristle | adult stage, with Scer\GAL4sca-109-68
anterior scutellar bristle | adult stage, with Scer\GAL4sca-109-68
anterior supraalar bristle | adult stage, with Scer\GAL4sca-109-68
cardioblast
cardioblast, with Scer\GAL4twi.PB
cardioblast | ectopic
cephalopharyngeal skeleton
crystal cell, with Scer\GAL4lz-gal4
crystal cell, with Scer\GAL4twi.PB
crystal cell | ectopic
crystal cell | embryonic stage, with Scer\GAL4lz-gal4
crystal cell | larval stage
crystal cell | third instar larval stage
cuticle
direct flight muscle
direct flight muscle (with Df(2L)ushrev18)
dorsal bridge
dorsal medial indirect flight muscle
dorsocentral bristle
dorsocentral bristle, with Scer\GAL4pnr-MD237
dorsocentral bristle & axon
dorsocentral bristle | cell non-autonomous | somatic clone
dorsocentral bristle | heat sensitive
dorsocentral bristle | precursor, with Scer\GAL4pnr-MD237
egg chorion | drug conditional, with Tn10\tetRrtTA.Act5C
embryo | dorsal closure stage
embryonic/larval lymph gland, with Scer\GAL4Mef2.PR
embryonic/larval lymph gland, with Scer\GAL4twi.PB
embryonic/larval lymph gland (with ushrev24)
embryonic/larval lymph gland (with ushVX22)
embryonic/larval lymph gland | larval stage
embryonic/larval lymph gland | second instar larval stage, with Scer\GAL4Cg.PA
embryonic/larval lymph gland | second instar larval stage (with ushrev24)
embryonic/larval lymph gland | second instar larval stage (with ushVX22)
embryonic/larval lymph gland | third instar larval stage
embryonic/larval lymph gland | third instar larval stage (with ushrev24)
embryonic/larval lymph gland | third instar larval stage (with ushVX22)
embryonic head
epidermis | anterior
epidermis | posterior
eye, with Scer\GAL4ey.PH
eye disc | heat sensitive, with Scer\GAL4ey.PH
fat body | somatic clone, with Scer\GAL4Act.PU
filzkorper
heart primordium, with Scer\GAL4twi.PB
hemocyte | larval stage
hemocyte | third instar larval stage, with Scer\GAL4Cg.PA
hemocyte | third instar larval stage (with ushrev24)
hemocyte | third instar larval stage (with ushVX22)
lamellocyte (with ushrev24)
lamellocyte (with ushVX22)
lamellocyte | larval stage
lamellocyte | third instar larval stage
lamellocyte | third instar larval stage, with Scer\GAL4Cg.PA
lamellocyte | third instar larval stage (with ushrev24)
lamellocyte | third instar larval stage (with ushVX22)
mesoderm
mitotic domain 1 | extended germ band stage
ommatidium, with Scer\GAL4ey.PH
pericardial cell | ectopic
plasmatocyte | third instar larval stage
plasmatocyte | third instar larval stage, with Scer\GAL4Cg.PA
plasmatocyte | third instar larval stage (with ushrev24)
plasmatocyte | third instar larval stage (with ushVX22)
posterior dorsocentral bristle | adult stage, with Scer\GAL4sca-109-68
posterior notopleural bristle | adult stage, with Scer\GAL4sca-109-68
posterior scutellar bristle | adult stage, with Scer\GAL4sca-109-68
posterior signalling center of lymph gland primary lobe, with Scer\GAL4kn-col85-GAL4
posterior supraalar bristle | adult stage, with Scer\GAL4sca-109-68
postvertical bristle
presutural bristle | adult stage, with Scer\GAL4sca-109-68
scutellar bristle
scutellum | somatic clone
vertical bridge
wing, with Scer\GAL4Bx-MS1096
wing | male limited, with Scer\GAL4Bx-MS1096
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\ZFPM1
6 of 15
Yes
Yes
Hsap\ZFPM2
5 of 15
No
Yes
Hsap\ZNF445
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Zfpm1
6 of 15
Yes
Yes
1  
Mmus\Zfpm2
5 of 15
No
Yes
2  
Mmus\Zfp445
1 of 15
No
No
Rattus norvegicus (Norway rat) (2)
Rnor\Zfpm1
5 of 13
Yes
Yes
Rnor\Zfpm2
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (3)
Xtro\zfpm1
4 of 12
Yes
Yes
Xtro\zfpm2
4 of 12
Yes
Yes
Xtro\znf341
1 of 12
No
Yes
Danio rerio (Zebrafish) (3)
Drer\zfpm2a
5 of 15
Yes
Yes
Drer\zfpm1
4 of 15
No
Yes
Drer\zfpm2b
4 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\F21A9.2
2 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091902K5 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00000053
Drosophila simulans
Dsim\GD23030
Drosophila sechellia
Dsec\GM16748
Drosophila erecta
Dere\GG24725
Drosophila yakuba
Dyak\GE16880
Drosophila ananassae
Dana\GF20666
Drosophila pseudoobscura pseudoobscura
Dpse\GA15455
Drosophila persimilis
Dper\GL18881
Drosophila willistoni
Dwil\GK24402
Drosophila virilis
Dvir\GJ17781
Drosophila mojavensis
Dmoj\GI21745
Drosophila grimshawi
Dgri\GH11230
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150132 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17014105
Glossina morsitans
Tsetse fly
Gmor\GMOY007015
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_06937
Mayetiola destructor
Hessian fly
Mdes\Mdes012384
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL003327
Anopheles darlingi
American malaria mosquito
Adar\ADAC003488
Anopheles gambiae
Malaria mosquito
Agam\AGAP009066
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ005812
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ005813
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W04O6 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA006027
Danaus plexippus
Monarch butterfly
Dple\DPOGS201552-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL010924
Apis florea
Little honeybee
Aflo\2228501908
Apis mellifera
Western honey bee
Amel\GB45131
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP15034
Bombus terrestris
Buff-tailed bumblebee
Bter\15212381
Linepithema humile
Argentine ant
Lhum\LH22570
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:0028b1
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG003305
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_03987
Tribolium castaneum
Red flour beetle
Tcas\TC031592
Tribolium castaneum
Red flour beetle
Tcas\ush
Pediculus humanus
Human body louse
Phum\PHUM248250
Rhodnius prolixus
Kissing bug
Rpro\RPRC004201
Cimex lectularius
Bed bug
Clec\CLEC005474
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI005951
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_14315
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X04KR )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR003843
Strigamia maritima
European centipede
Smar\SMAR003842
Ixodes scapularis
Black-legged tick
Isca\ISCW003495
Stegodyphus mimosarum
African social velvet spider
Smim\KFM60082.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM77022.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur13g03940
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_311572
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0GCP )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Z227
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Z102
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Z28
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Z250
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 3 )
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    ushVDRC.cUa
    exacerbates  hematologic cancer
    modeled by hopTum
    (Bausek and Zeidler, 2014)
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    ZFPM1; zinc finger protein, FOG family member 1
    6 of 15
    601950
        ZFPM2; zinc finger protein, FOG family member 2
        5 of 15
        603693
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Summary of Genetic Interactions
        esyN Network Diagram
        esyN Network Key:
        Suppression
        Enhancement
        View in Interactions Browser
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        ush
        enhanceable
        htl
        (Fossett et al., 2000)
        ush
        suppressible
        srp
        (Sorrentino et al., 2007)
        ush
        enhanceable
        srp
        (Fossett et al., 2003, Goldman-Levi et al., 1996)
        ush
        enhanceable
        tup
        (Goldman-Levi et al., 1996)
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Chi
        enhanceable
        ush
        (Biryukova and Heitzler, 2008)
        CtBP
        suppressible
        ush
        (Stern et al., 2009)
        hop
        enhanceable
        ush
        (Bausek and Zeidler, 2014)
        hop
        suppressible
        ush
        (Sorrentino et al., 2007)
        htl
        enhanceable
        ush
        (Fossett et al., 2000)
        L
        suppressible
        ush
        (Singh et al., 2005)
        mir-8
        suppressible
        ush
        (Hyun et al., 2009)
        peb
        enhanceable
        ush
        (Goldman-Levi et al., 1996)
        pnr
        enhanceable
        ush
        (Cubadda et al., 1997)
        srp
        enhanceable
        ush
        (Goldman-Levi et al., 1996)
        srp
        suppressible
        ush
        (Fossett et al., 2003)
        tup
        enhanceable
        ush
        (Goldman-Levi et al., 1996)
        External Data
        Subunit Structure (UniProtKB)
        Interacts with pnr, although weak this interaction is essential. Interacts with the isoform SrpNC of srp. Interacts with CtBP corepressor.
        (UniProt, Q9VPQ6 )
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        External Data
        Linkouts
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        2L
        Recombination map
        2-1.5
        Cytogenetic map
        21E1-21E2
        Sequence location
        2L:476,220..540,560 [+]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        21E1-21E2
        Limits computationally determined from genome sequence between P{lacW}l(2)k09610k09610&P{lacW}Sk09538a and P{PZ}l(2)1068510685&P{lacW}Tango14k00619
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location

        2-1.5

        (FlyBase, 2016)

        2-0.1

        (Comeron et al., 2012)

        2-0

        (Tearle and Nusslein-Volhard, 1987)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (29)
        Genomic Clones (37)
        cDNA Clones (29)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         

        monoclonal

        (Cubadda et al., 1997)
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of

        Source for identity of: ush CG2762

        (Guan, 1999.11)
        Source for database merge of
        Additional comments
        Other Comments

        S2-derived S2-NP cells are treated with dsRNA made from templates generated with primers directed against ush to assess the role of this gene in Stat92E tyrosine phosphorylation.

        (Baeg et al., 2005)

        ush is required for os-induced Stat92E phosphorylation.

        (Baeg et al., 2005)

        ush is a negative regulator of hemocoel, heart and eye development.

        (Fossett et al., 2001)

        Identified as a gene with significant level of mRNA cycling as assessed by expression analysis using high density oligonucleotide arrays with probe generated from adult heads harvested over six time points over the course of a day.

        (McDonald and Rosbash, 2001)

        ush has a critical role in several diverse processes of mesoderm differentiation and heart formation.

        (Fossett et al., 2000)

        The pnr/ush complex serves as a repressor and a transcriptional activator, respectively for wg and ush expression.

        (Sato and Saigo, 2000)

        The dpp gradient may regulate notal wg expression through induction of pnr and ush.

        (Tomoyasu et al., 2000)

        pnr and ush are main effectors of the regulation of wg expression in the presumptive notum.

        (Garcia-Garcia et al., 1999)

        pnr and ush provide positional information for the patterning of the dorsocentral mechanosensory bristle cluster.

        (Garcia-Garcia et al., 1999)

        ush encodes a zinc-finger protein that regulates the proneural genes ac and sc during bristle formation.

        (Cubadda et al., 1997)

        A member of the ush-group of genes which are required for maintenance of the amnioserosa once it has differentiated.

        (Frank and Rushlow, 1996)

        Mutants affect amnioserosa differentiation as well as germ band retraction.

        (Frank and Rushlow, 1995)

        Mutations in zygotic gene ush do not interact with RpII140wimp.

        (Parkhurst and Ish-Horowicz, 1991)

        Zygotically active locus involved in the terminal developmental program in the embryo.

        (Strecker et al., 1991)
        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 60 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        modMine - A data warehouse for the modENCODE project
        PDB - An information portal to biological macromolecular structures
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyMine - An integrated database for Drosophila genomics
        Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Synonyms and Secondary IDs (14)
        Reported As
        Symbol Synonym
        CG2762
        (Schertel et al., 2013, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Khokhar et al., 2008)
        FOG
        (Vlisidou and Wood, 2015, Fossett and Schulz, 2001)
        USH
        (Lehmann, 2018, Lee et al., 2015, Hyun, 2013)
        Ush
        (Yu et al., 2018, Van Bortle et al., 2015, Gao et al., 2014, Honti et al., 2014, Lee and Hyun, 2014, Wang et al., 2014, Amoyel and Bach, 2012, Chlon and Crispino, 2012, Tokusumi et al., 2010, Tokusumi et al., 2009, Frandsen et al., 2008, Gesellchen et al., 2005, Fossett et al., 2001)
        ZFPM2
        (Gieger et al., 2011)
        dFOG
        (Fossett, 2013, Liew et al., 2005)
        l(2)19
        u-sh
        (Haenlin et al., 1995)
        ush
        (Creed et al., 2020, Hoppe et al., 2020, Lepesant et al., 2020, Overton et al., 2020, Soluri et al., 2020, Banerjee et al., 2019, Michalak et al., 2019, Baldeosingh et al., 2018, Bischof et al., 2018, Rajpurohit et al., 2018, Karaiskos et al., 2017, Liu et al., 2017, Transgenic RNAi Project members, 2017-, Brookheart and Duncan, 2016, Clandinin and Owens, 2016-, Gao et al., 2016, Gomez et al., 2016, Sandler and Stathopoulos, 2016, Sandler and Stathopoulos, 2016, Duff et al., 2015, Morán et al., 2015, Newton et al., 2015, Sawala et al., 2015, Schertel et al., 2015, Vedanayagam and Garrigan, 2015, Vlisidou and Wood, 2015, Winstanley et al., 2015, Bausek and Zeidler, 2014, Bischof, 2014.10.20, Gonzalez et al., 2014, Kim and Choe, 2014, Salazar-Jaramillo et al., 2014, Valanne, 2014, de Celis et al., 2013.9.11, Saunders et al., 2013, Schertel et al., 2013, Amoyel and Bach, 2012, Belozerov et al., 2012, Garcia et al., 2012, Hainaut et al., 2012, Hironaka et al., 2012, Holmqvist et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Jin et al., 2012, Kennell et al., 2012, Lada et al., 2012, Gao et al., 2011, Gieger et al., 2011, Jungreis et al., 2011, Avet-Rochex et al., 2010, Campos et al., 2010, Fromental-Ramain et al., 2010, Heitzler, 2010.5.12, Pauli et al., 2010, Simone and DiNardo, 2010, Sorrentino, 2010, Stofanko et al., 2010, Boettiger and Levine, 2009, Gao et al., 2009, Hyun et al., 2009, Stern et al., 2009, Wheeler et al., 2009, Biryukova and Heitzler, 2008, Khokhar et al., 2008, Kwon et al., 2008, Kwong et al., 2008, Lee et al., 2008, Miles et al., 2008, Wang et al., 2008, Zhou et al., 2008, Baeg et al., 2007, de Navascués and Modolell, 2007, Fernández et al., 2007, Lecuyer et al., 2007, Letizia et al., 2007, Liang et al., 2007, Sorrentino et al., 2007, Tokusumi et al., 2007, Wada et al., 2007, Mizutani et al., 2006, Sandmann et al., 2006, Sellin et al., 2006, Simpson et al., 2006, Scuderi and Letsou, 2005, Stathopoulos and Levine, 2005)
        ushaped
        (Fossett and Schulz, 2001)
        Name Synonyms
        Drosophila Friend of GATA
        (Muratoglu et al., 2006)
        Friend of GATA
        (Sorrentino et al., 2007)
        U-shaped
        (Ramond et al., 2020, Yu et al., 2018, Gao et al., 2016, Kanoh et al., 2015, Lucas et al., 2015, Gao et al., 2014, Wang et al., 2014, Lada et al., 2012, Fromental-Ramain et al., 2010, Tokusumi et al., 2010, Stern et al., 2009, Biryukova and Heitzler, 2008, Frandsen et al., 2008, Letizia et al., 2007, Muratoglu et al., 2007, Sorrentino et al., 2007, Tokusumi et al., 2007, Gesellchen et al., 2005)
        u-shaped
        (Abhyankar et al., 2018, Brookheart and Duncan, 2016, Gomez et al., 2016, Wieschaus and Nüsslein-Volhard, 2016, Newton et al., 2015, Sawala et al., 2015, Vedanayagam and Garrigan, 2015, Winstanley et al., 2015, Amoyel and Bach, 2012, Hazelett et al., 2012, Holmqvist et al., 2012, Jin et al., 2012, Kennell et al., 2012, Avet-Rochex et al., 2010, Campos et al., 2010, Simone and DiNardo, 2010, Sorrentino, 2010, Valanne et al., 2010, Boettiger and Levine, 2009, Gao et al., 2009, Hyun et al., 2009, Miles et al., 2008, Wang et al., 2008, Fernández et al., 2007, Muratoglu et al., 2007, Muratoglu et al., 2006, Simpson et al., 2006, Scuderi and Letsou, 2005, Haenlin, 1997.4.5)
        ushaped
        (Khokhar et al., 2008)
        Secondary FlyBase IDs
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          References (273)