PP2A, PP2A-C, PP2Ac, protein phosphatase 2A, PP2A catalytic subunit
Gene model reviewed during 5.52
Low-frequency RNA-Seq exon junction(s) not annotated.
309 (aa); 35.42 (kD predicted)
Reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization (By similarity).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mts using the Feature Mapper tool.
The 1.5kb mts transcript is strongly expressed in eary embryos and decreases about 60% in late embryos and larvae. It is also detected in adults.
The 2.3kb mts transcript is strongly expressed in early embryos and is reduced ~20fold in late embryos and larvae.
Expression was observed in cryostat sections at several different stages of development. In adult females, basal levels of Pp2A-29B transcripts are detected in all tissues. High levels of expression are observed in the ovary in the nurse cells and oocytes. Slightly elevated levels are observed in the brain and the gut. In early embryos, transcripts are evenly distributed at high levels. In later embryos, the levels decline but slightly higher levels are seen in the developing CNS than in surrounding tissue. In third instar larvae, transcripts are enriched in the supraesophageal ganglion but are at very low levels in the neuropil. Elevated levels are observed in imaginal discs and salivary glands. In early embryos, in situ hybridization reveals uniform expression. Enrichment is observed in neuroblasts at germ band extension and in the ventral nerve cord and supraesophageal ganglion at later embryonic stages. In addition elevated levels are observed in the gonads.
Pp2A-29B and mts proteins show the same cytoplasmic localization in embryos and imaginal discs as the tws protein. In syncytial blastoderm embryos the protein is found in the cytoplasmic region surrounding the cortical layer of nuclei. The protein is cytoplasmic in pole cells. In cycle 14 embryos, the protein is located throughout the cytoplasm of interphase cells, and throughout the whole cell as it progresses through mitosis. In dividing third larval instar brain neuroblasts, the protein is detected in the cytoplasm of interphase and mitotic cells.
GBrowse - Visual display of RNA-Seq signalsView Dmel\mts in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: mts CG7109
dsRNA directed against this gene has been used in a screen for genes required for constitutive protein secretion.
S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.
Identified as a potential component of the hh signalling pathway in a genome-wide RNAi screen. dsRNA made from templates generated with primers directed affects the extent of expression of a hh signaling reporter construct in Clone 8 cells.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 cells: change from round to spindle-shaped, with the formation of F-actin puncta and microtubule extensions. Alterations of cell shape are also evident in S2R+ cells; cells become round and detached.
Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.
Lack of mts causes a block in mitosis and uncouples the centrosome cycle from the nuclear cycle and prevents the attachment of microtubules to the kinetochore.
Encodes the catalytic subunit of PP2A.
mts has been molecularly cloned and developmental expression patterns examined.