FlyBase Gene Report: Dmel\Hrb87F

General Information

Symbol

Dmel\Hrb87F

Species

D. melanogaster

Name

Heterogeneous nuclear ribonucleoprotein at 87F

Annotation Symbol

CG12749

Feature Type

protein_coding_gene

FlyBase ID

FBgn0004237

Gene Model Status

Current

Stock Availability

11 publicly available

Gene Summary

Heterogeneous nuclear ribonucleoprotein at 87F (Hrb87F) encodes a hnRNP-A family RNA-binding protein, involved in gene expression and RNA processing. It is an essential component of the nucleoplasmic omega speckles and necessary for telomere maintenance. Hrb87F null mutants show defects in development, female fertility and stress tolerance. [Date last reviewed: 2018-09-06] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

hrp36, P11, hnRNPA1, Hrb87Fa, Q16

Key Links

Genomic Location

Cytogenetic map

87F7-87F7

Sequence location

3R:13,656,846..13,660,531 [-]

Recombination map

3-54

RefSeq locus

NT_033777 REGION:13656846..13660531

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (20 terms)

Molecular Function (6 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables sequence-specific DNA binding

inferred from direct assay

(Borah et al., 2009)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

enables mRNA 3'-UTR binding

inferred from biological aspect of ancestor with PANTHER:PTN002689981

(GO Reference Genome Project, 2011-)

enables mRNA binding

inferred from sequence or structural similarity

(Lasko, 2000)

enables nucleic acid binding

inferred from electronic annotation with InterPro:IPR035979

(InterPro Project Members, 2004-)

enables poly(G) binding

inferred from biological aspect of ancestor with PANTHER:PTN002689981

(GO Reference Genome Project, 2011-)

enables RNA binding

inferred from electronic annotation with InterPro:IPR000504

(InterPro Project Members, 2004-)

Biological Process (7 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in compound eye morphogenesis

inferred from genetic interaction with UniProtKB:Q92879

(Sofola et al., 2007)

involved_in female gonad development

inferred from mutant phenotype

(Singh and Lakhotia, 2012)

involved_in mitotic cell cycle

inferred from high throughput mutant phenotype

(Ducat et al., 2008)

involved_in regulation of alternative mRNA splicing, via spliceosome

inferred from high throughput mutant phenotype

(Park et al., 2004)

inferred from mutant phenotype

(Borah et al., 2009)

involved_in response to heat

inferred from mutant phenotype

(Singh and Lakhotia, 2012)

involved_in response to starvation

inferred from mutant phenotype

(Singh and Lakhotia, 2012)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in positive regulation of mRNA splicing, via spliceosome

inferred from biological aspect of ancestor with PANTHER:PTN002689981

(GO Reference Genome Project, 2011-)

Cellular Component (7 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

located_in chromatin

inferred from direct assay

(Prasanth et al., 2000)

located_in heterochromatin

inferred from direct assay

(Swenson et al., 2016)

located_in nucleoplasm

inferred from direct assay

(Matunis et al., 1992)

located_in nucleus

inferred from direct assay

(Prasanth et al., 2000)

located_in omega speckle

inferred from direct assay

(Prasanth et al., 2000)

located_in polytene chromosome puff

inferred from direct assay

(Buszczak and Spradling, 2006, Matunis et al., 1992)

part_of ribonucleoprotein complex

inferred from direct assay

(Matunis et al., 1992)

inferred from physical interaction with FLYBASE:sqd; FB:FBgn0263396

(Matunis et al., 1992)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN004470902

(GO Reference Genome Project, 2011-)

located_in nucleus

inferred by curator from GO:0000381

(Park et al., 2004)

part_of ribonucleoprotein complex

inferred from sequence or structural similarity with UniProtKB:P09651

(Matunis et al., 1992)

Gene Group (FlyBase)

SPLICEOSOME COMPLEX A

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

SPLICEOSOME COMPLEX A -: Nuclear pre-mRNA splicing is catalyzed by the spliceosome which assembles in a step-wise manner. Complex A, the pre-spliceosome, is composed of U1 and U2 snRNPs and proteins involved in recognition of the 5' splice site and branch point. (Adapted from PMID:24452469 and PMID:23118483).

Protein Function (UniProtKB)

This protein is a component of ribonucleosomes. Could be needed to organize a concentration gradient of a dorsalizing morphogen (Dm) originating in the germinal vesicle.

(UniProt, P48810)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Hrb2: hnRNA-binding protein 2 (S. Haynes)

Homology to the mammalian A and B hnRNP proteins based on amino acid sequence inferred from nucleotide sequence.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Hrb87F for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P48810)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.43

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Hrb87F-RA

FBtr0082851

NM_057459

2281

385

Hrb87F-RC

FBtr0300590

NM_001170131

1810

385

Hrb87F-RD

FBtr0310621

NM_001275609

1587

385

Hrb87F-RE

FBtr0334495

NM_057458

2759

385

Additional Transcript Data and Comments

Reported size (kB)

1.1 (longest cDNA)

(Matunis et al., 1992)

2.2, 1.7 (northern blot)

(Haynes et al., 1991)

2.3, 1.8 (compiled cDNA)

(Hovemann et al., 1991)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Hrb87F-PA

FBpp0082316

39.5

385

9.46

FBpp0289817

39.5

385

9.46

FBpp0302741

39.5

385

9.46

FBpp0306562

39.5

385

9.46

P48810

NP_476806

AAN13574

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

385 aa isoforms: Hrb87F-PA, Hrb87F-PC, Hrb87F-PD, Hrb87F-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

36 (kD observed)

(Matunis et al., 1992)

326 (aa); 34 (kD observed); 34 (kD predicted)

(Matunis et al., 1992)

386 (aa); 40 (kD predicted)

(Haynes et al., 1991)

386 (aa); 38 (kD observed); 40 (kD predicted)

(Hovemann et al., 1991)

Comments

One of a couple of protein products.

(FlyBase, 1992-)

hnRNP-specific mAbs were obtained and used to purify hnRNP complexes from S2 cells. hnRNP proteins were identified on the basis of nucleoplasmic localization, association with nascent transcripts, crosslinking to poly(A)-containing RNA in living cells, and amino acid sequence. Staining of polytene chromosomes reveals that Hrb87F protein is associated with actively transcribing loci.

(Matunis et al., 1992)

Hrb87F protein has two amino-terminal RNP-CS RNA-binding domains and a carboxy-terminal glycine-rich auxiliary domain.

(Matunis et al., 1992)

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Hrb87F using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.40

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13 -- 16

larval midline neuron

(Kearney et al., 2004)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Haynes et al., 1991)

oogenesis & adult stage | female

ovary

(Haynes et al., 1991)

Additional Descriptive Data

2.3kb Hrb87F transcripts are expressed throughout development. Transcripts are abundant in ovaries and early embryos, decline in late embryos and early larvae, and then increase again from the second larval instar stage.

(Haynes et al., 1991)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

ovary

(Pisa et al., 2009)

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in chromatin

inferred from direct assay

(Prasanth et al., 2000)

located_in heterochromatin

inferred from direct assay

(Swenson et al., 2016)

located_in nucleoplasm

inferred from direct assay

(Matunis et al., 1992)

located_in nucleus

inferred from direct assay

(Prasanth et al., 2000)

located_in omega speckle

inferred from direct assay

(Prasanth et al., 2000)

located_in polytene chromosome puff

inferred from direct assay

(Buszczak and Spradling, 2006, Matunis et al., 1992)

part_of ribonucleoprotein complex

inferred from direct assay

(Matunis et al., 1992)

inferred from physical interaction with FLYBASE:sqd; FB:FBgn0263396

(Matunis et al., 1992)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Hrb87F in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Hrb87F

View exonic expression by tissue for Dmel\Hrb87F

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

48535/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004237

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004237

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004237

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12749

Flygut - An atlas of the Drosophila adult midgut

FBgn0004237

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 9 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 14 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Hrb87F

Transgenic constructs containing regulatory region of Hrb87F

Aberrations (Deficiencies and Duplications) ( 10 )

Inferred from experimentation ( 10 )

Gene not disrupted in

Df(3R)red3l

(Peng and Mount, 1995)

Df(3R)ry27

(Peng and Mount, 1995)

Inferred from location ( 3 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^insc-Mz1407

Hrb87F^GD7818

lethal - all die before end of larval stage, with Scer\GAL4^elav.PLu

Hrb87F^GS15926

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Hrb87F^GD7818

viable, with Scer\GAL4^tin.CΔ4

Hrb87F^GD7818

Other Phenotypes

Allele

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav.PU

Hrb87F^KK108162

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

Hrb87F^GD7818

visible | adult stage, with Scer\GAL4^GMR.PU

Hrb87F^KK108162

Phenotype manifest in

Allele

eye, with Scer\GAL4^GMR.PU

Hrb87F^KK108162

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

Hrb87F^GD7818

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

Hrb87F^GD7818

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (71)

Hsap\HNRNPA1L2

11 of 14

Yes

Hsap\HNRNPA1

10 of 14

Yes

Hsap\HNRNPA2B1

10 of 14

Yes

Hsap\HNRNPA3

9 of 14

Yes

Hsap\HNRNPA1P48

8 of 14

Yes

Hsap\HNRNPA0

3 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\RBMXL3

1 of 14

Yes

Hsap\RBMY1A1

1 of 14

Hsap\RBMY1B

1 of 14

Hsap\RBMY1C

1 of 14

Yes

Hsap\RBMY1D

1 of 14

Hsap\RBMY1E

1 of 14

Yes

Hsap\RBMY1F

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

143

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (39)

Rnor\Hnrnpa1

11 of 14

Yes

Rnor\Hnrnpa2b1

10 of 14

Yes

Rnor\Hnrnpa3

9 of 14

Yes

Rnor\LOC100911361

8 of 14

Yes

Rnor\LOC681410

3 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (47)

Mmus\Hnrnpa1

11 of 14

Yes

Mmus\Hnrnpa2b1

10 of 14

Yes

Mmus\Hnrnpa3

9 of 14

Yes

Mmus\Gm17190

6 of 14

Yes

Mmus\Hnrnpa0

3 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Gm7324

1 of 14

Mmus\Gm10256

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (32)

Xtro\hnrnpa3

9 of 13

Yes

Xtro\hnrnpa1

8 of 13

Yes

Xtro\hnrnpa2b1

6 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (41)

Drer\hnrnpa1b

11 of 14

Yes

Drer\hnrnpa1a

10 of 14

Yes

Drer\hnrnpa3

9 of 14

Yes

Drer\hnrnpa0l

5 of 14

Yes

Drer\hnrnpa0a

3 of 14

Yes

Drer\hnrnpa0b

3 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (30)

Cele\hrpa-1

10 of 14

Yes

Cele\hrpa-2

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (37)

Agam\AgaP_AGAP002374

10 of 12

Yes

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (91)

Atha\AT3G13224

3 of 13

Yes

Atha\AT5G40490

3 of 13

Yes

Atha\AT1G01080

2 of 13

Yes

Atha\AT1G17640

2 of 13

Atha\AT1G60000

2 of 13

Atha\AT2G22100

2 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

Yes

Atha\AT3G07810

2 of 13

Atha\AT3G15010

2 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Yes

Atha\NUC-L2

2 of 13

Yes

Atha\PHIP1

2 of 13

Yes

Atha\RBP31

2 of 13

Atha\UBA1A

2 of 13

Yes

Atha\UBA2A

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT4G36960

1 of 13

Atha\AT5G03495

1 of 13

Yes

Atha\AT5G03580

1 of 13

Atha\AT5G06210

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT5G46840

1 of 13

Atha\AT5G47620

1 of 13

Atha\AT5G51730

1 of 13

Yes

Atha\AT5G52545

1 of 13

Atha\AT5G53680

1 of 13

Atha\AT5G53700

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\LIF2

1 of 13

Atha\ML5

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (8)

Scer\HRP1

3 of 13

Yes

Scer\NOP12

1 of 13

Scer\NOP13

1 of 13

Yes

Scer\NSR1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (12)

Spom\SPBC660.15

3 of 12

Yes

Spom\gar2

2 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Hrb87F. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (41)

12 of 13

9 of 13

4 of 13

3 of 13

3 of 13

sqd

3 of 13

Caper

2 of 13

CG34354

2 of 13

msi

2 of 13

SLIRP1

2 of 13

SLIRP2

2 of 13

Sxl

2 of 13

Syp

2 of 13

bol

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

fne

1 of 13

hfp

1 of 13

lark

1 of 13

LS2

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Spx

1 of 13

ssx

1 of 13

TBPH

1 of 13

trv

1 of 13

U2af50

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 4 )

Human Ortholog

Disease

Evidence

References

HNRNPA1; heterogeneous nuclear ribonucleoprotein A1

model of inclusion body myopathy with early-onset Paget disease of bone with or without frontotemporal dementia 3

IEA

(FlyBase, 2019-)

model of distal myopathy 3

IEA

(FlyBase, 2019-)

model of amyotrophic lateral sclerosis type 20

IEA

(FlyBase, 2019-)

HNRNPA2B1; heterogeneous nuclear ribonucleoprotein A2/B1

model of inclusion body myopathy with early-onset Paget disease of bone with or without frontotemporal dementia 2

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 4 )

Allele

Disease

Interaction

References

Hrb87F^UAS.cSa

ameliorates fragile X-associated tremor/ataxia syndrome

modeled by Zzzz\CGG^90.UAS.EGFP

(Sofola et al., 2007)

modeled by Zzzz\CGG^60.UAS.EGFP

(Tan et al., 2012)

Hrb87F^JF01757

exacerbates neurodegenerative disease

modeled by Zzzz\CGG^90.UAS.EGFP

(He et al., 2014)

Hrb87F^KK108162

DOES NOT ameliorate spinocerebellar ataxia type 31

modeled by Zzzz\UGGAA^W.UAS

(Ishiguro et al., 2017)

modeled by Zzzz\UGGAA^W.UAS, Hsap\TARDBP^UAS.cIb

(Ishiguro et al., 2017)

modeled by Zzzz\UGGAA^S.UAS

(Ishiguro et al., 2017)

modeled by Zzzz\UGGAA^S.UAS, Hsap\TARDBP^UAS.cIb

(Ishiguro et al., 2017)

exacerbates frontotemporal dementia and/or amyotrophic lateral sclerosis 1

modeled by Zzzz\poly-GR^50.UAS.EGFP

(Lee et al., 2016)

modeled by Zzzz\GGGGCC^89.UAS

(Taminato et al., 2023)

Hrb87F^RNAi.UAS.cUa

exacerbates amyotrophic lateral sclerosis type 6

modeled by Hsap\FUS^UAS.cMa

(An et al., 2022)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

HNRNPA1L2; heterogeneous nuclear ribonucleoprotein A1 like 2

11 of 14

HNRNPA1; heterogeneous nuclear ribonucleoprotein A1

10 of 14

164017

HNRNPA2B1; heterogeneous nuclear ribonucleoprotein A2/B1

10 of 14

600124

inclusion body myopathy with early-onset Paget disease of bone with or without frontotemporal dementia 2

HNRNPA3; heterogeneous nuclear ribonucleoprotein A3

9 of 14

605372

HNRNPA1L3; heterogeneous nuclear ribonucleoprotein A1 like 3

8 of 14

HNRNPA0; heterogeneous nuclear ribonucleoprotein A0

3 of 14

609409

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Hrb87F - HnRNP-K

anti bait coimmunoprecipitation, western blot

(Hovemann et al., 2000, Prasanth et al., 2000)

Hrb87F - Hrb27C

pull down, autoradiography

(Horan et al., 2015)

Hrb87F - Hrb98DE

experimental knowledge based

(Guruharsha et al., 2011)

Hrb87F - Mgtor

anti bait coimmunoprecipitation, western blot

(Singh and Lakhotia, 2015)

Hrb87F - Polr2A

anti bait coimmunoprecipitation, western blot

(Singh et al., 2019)

Hrb87F - Raf

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Hrb87F - Saf-B

anti tag coimmunoprecipitation, western blot

(Singh and Lakhotia, 2015)

Hrb87F - Src42A

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Hrb87F - Su(var)205

anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation

(Tan et al., 2012, Piacentini et al., 2009)

Hrb87F - TBPH

anti bait coimmunoprecipitation, western blot

(Lo Piccolo et al., 2018)

Hrb87F - glo

experimental knowledge based

(Guruharsha et al., 2011)

Hrb87F - mle

anti tag coimmunoprecipitation, Identification by mass spectrometry, western blot

(Cugusi et al., 2015)

Hrb87F - nej

anti bait coimmunoprecipitation, western blot

(Mallik and Lakhotia, 2010)

Hrb87F - orb2

anti tag coimmunoprecipitation, western blot

(Khan et al., 2015)

Hrb87F - sqd

anti bait coimmunoprecipitation, molecular weight estimation by autoradiography, western blot, molecular weight estimation by staining

(Prasanth et al., 2000, Amero et al., 1993, Matunis et al., 1992)

RNA-protein

Physical Interaction

Assay

References

Hrb87F - Dscam1

electrophoretic mobility shift assay, autoradiography, anti bait coimmunoprecipitation, nucleotide sequence identification

(Olson et al., 2007)

Hrb87F - lncRNA:Hsrω

pull down, anti tag western blot

(Onorati et al., 2011)

Hrb87F - pros

pull down, Identification by mass spectrometry

(Borah et al., 2009)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Hrb87F

enhanceable

TBPH

(Appocher et al., 2017)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

TBPH

suppressible

Hrb87F

(Appocher et al., 2017)

TBPH

enhanceable

Hrb87F

(Appocher et al., 2017)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

71477

DroID - A comprehensive database of gene and protein interactions.

FBgn0004237

MIST (protein-protein) - An integrated Molecular Interaction Database

48535

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Spliceosome

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-54

Cytogenetic map

87F7-87F7

Sequence location

3R:13,656,846..13,660,531 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

87F7-87F7

Limits computationally determined from genome sequence between P{PZ}CtBP⁰³⁴⁶³ and P{lacW}B52^s2249&P{lacW}flfl^L4179

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

85C-85D

(Hovemann, 1991.2.28)

87F-87F

(determined by in situ hybridisation)

(Matunis et al., 1992, Haynes et al., 1991)

Experimentally Determined Recombination Data

Location

3-54

(FlyBase, 2016)

3-51.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (11)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

12869

w¹¹¹⁸; P{GT1}Hrb87F^BG02743

31244

y¹ v¹; P{TRiP.JF01757}attP2

31472

y¹ v¹; P{TRiP.JF01250}attP2/TM3, Ser¹

52937

y¹ sc^* v¹ sev²¹; P{TRiP.HMC03679}attP40

14414

y¹ w^67c23; ry⁵⁰⁶ P{SUPor-P}Hrb87F^KG02089

99683

w^*; Df(3R)B52²⁸, Hrb87F²⁸ B52²⁸/TM6B, Tb¹

Kyoto

206416

y¹ w^67c23; P{GSV6}GS15926/TM3, Sb¹ Ser¹

12749R-1

v100732

v51759

w¹¹¹⁸; P{GD7818}v51759

v318186

PBac{fTRG00688.sfGFP-TVPTBF}VK00033

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (130)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

MIP15350

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0004237

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0004237

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Blanchette et al., 2009, Borah et al., 2009, Hovemann et al., 1991)

monoclonal

(Matunis et al., 1992, Saumweber et al., 1980)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Loss of Hrb87F slows development, with a significant reduction in adult life span, decreased female fecundity and high sensitivity to starvation and thermal stresses.

(Singh and Lakhotia, 2012)

The consequences of HRB overexpression and absence in living flies is examined. Overexpression of Hrb87F does alter the splicing of Ddc pre-mRNA, indicating the overexpressed protein can reproduce the in vitro effect of excess protein.

(Zu et al., 1996)

Complete deletion of Hrb87F has no obvious defect on viability or fertility, but when in combined with hypodosage of Hrb98DE leads to reduced male and female fertility.

(Haynes et al., 1995)

The distribution of the hnRNP protein Hrb87F on nascent transcripts from polytene chromosomes has been studied.

(Matunis et al., 1993)

An Hrb87F cDNA clone has been isolated and sequenced.

(Matunis et al., 1992)

Isolated from an early embryonic cDNA library under reduced stringency conditions, using an Hrb98DE cDNA probe. Some Canton S strains have a 412 element insertion in the 3' untranslated region.

(Haynes et al., 1991)

Hrb87F has been cloned and sequenced.

(Haynes et al., 1991, Hovemann et al., 1991)

412 element inserted in 3' UTR in some Canton S lines

Homology to the mammalian A and B hnRNP proteins based on amino acid sequence inferred from nucleotide sequence.

Relationship to Other Genes

Source for database merge of

Source for merge of: Hrb87F CG12749

(Bayraktaroglu, 2000.4.12)

Source for merge of: Hrb87F Hrb85CD

(Mount, 2000.10.26)

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (26)

Reported As

Symbol Synonym

CG12749

(Appocher et al., 2017, Kolkhof et al., 2017, Brooks et al., 2015, Horan et al., 2015, Rosa-Ferreira et al., 2015, Cammarato et al., 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Pisa et al., 2009, Andersen and Tapon, 2008, Ducat et al., 2008, Ni et al., 2008, Olson et al., 2007, McIntyre et al., 2006, Brody et al., 2002)

HRB87F

(Yadav and Tapadia, 2016, Akanksha et al., 2008, Sofola et al., 2007, Lakhotia, 2003)

HRB87F/hrp36

(Beyer et al., 1996)

HRP36

(Turnage et al., 2000)

Hrb2

Hrb85CD

Hrb87F

(Finger et al., 2023, An et al., 2022, Lama et al., 2022, Kao et al., 2021, Cacchione et al., 2020, Meyer-Nava et al., 2020, Park et al., 2020, Sapiro et al., 2020, Berson et al., 2019, Ray et al., 2019, Azuma et al., 2018, Lo Piccolo et al., 2018, Appocher et al., 2017, Ishiguro et al., 2017, Ji and Tulin, 2016, Lee et al., 2016, Li et al., 2016, Sarov et al., 2016, Tan et al., 2016, Zhang et al., 2016, Brooks et al., 2015, Horan et al., 2015, Khan et al., 2015, Li et al., 2015, He et al., 2014, Kwon et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Tan et al., 2012, Friedman et al., 2011, Manita et al., 2011, Neumüller et al., 2011, Shukla and Tapadia, 2011, Baig et al., 2010, Mallik and Lakhotia, 2010, Hurt et al., 2009, Mallik and Lakhotia, 2009, Mallik and Lakhotia, 2009, Piacentini et al., 2009, Pisa et al., 2009, Buszczak et al., 2007, Olson et al., 2007, Rasmussen and Kellis, 2007, Sofola et al., 2007, Jolly and Lakhotia, 2006, McIntyre et al., 2006, Sengupta and Lakhotia, 2006, Reim et al., 1999)

Hrb87Fa

(Burnette et al., 1999)

Hrb87f

(Onorati et al., 2011, Sofola et al., 2007, Clouse and Schupbach, 2003)

Hrp36

(Ng and Kurtz, 2020, Ustaoglu et al., 2019, Lakhotia et al., 2012, Singh and Lakhotia, 2012, Venables et al., 2012, Verma et al., 2012, Hessle et al., 2009, Lakhotia et al., 1999)

P11

(Buszczak and Spradling, 2006, Hovemann et al., 2000, Lakhotia et al., 1999, Hovemann, 1991.2.28, Hovemann, 1991.2.28, Hovemann et al., 1991, Saumweber et al., 1980)

P11/Hrb87F

(Reim et al., 1999)

Q14

(Lakhotia et al., 1999)

Q16

(Lakhotia et al., 1999, Saumweber et al., 1980)

hnRNP A2/B1

(Tan et al., 2012)

hnRNP36

(Ni et al., 2006)

hnRNPA1

(Singh et al., 2019, Jolly and Lakhotia, 2006, Sengupta and Lakhotia, 2006, Lakhotia, 2003)

hnRNPA1/A2

(Romano et al., 2014)

hnRNPA2

(Jolly and Lakhotia, 2006, Lakhotia, 2003)

hnRNPA2/B1

(Castro et al., 2020)

hrb87f

(Ramesh et al., 2020)

hrp36

(Li, 2021, Ghanim et al., 2020, Xu et al., 2019, Azuma et al., 2018, Ji and Tulin, 2016, Horan et al., 2015, Venables et al., 2012, Blanchette et al., 2009, Borah et al., 2009, Graveley et al., 2008, Olson et al., 2007, Sofola et al., 2007, Park et al., 2004, Amero et al., 1993, Matunis et al., 1993, Weeks et al., 1993, Matunis et al., 1992, Matunis et al., 1992)

p38

(Reim et al., 1999)

Name Synonyms

Heterogeneous nuclear ribonucleoprotein at 85CD

Heterogeneous nuclear ribonucleoprotein at 87F

(Cammarato et al., 2011, Baig et al., 2010, Pisa et al., 2009)

hnRNA-binding protein 2

Secondary FlyBase IDs

FBgn0010230

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 52 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

48535

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P48810

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P48810

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

E1JIK0

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P48810

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

48535/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004237

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004237

FlyMine - An integrated database for Drosophila genomics

FBgn0004237

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG12749

MARRVEL_MODEL - MARRVEL (model organism gene)

48535

Linkouts

BioGRID - A database of protein and genetic interactions.

71477

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0004237

DroID - A comprehensive database of gene and protein interactions.

FBgn0004237

DRSC - Results frm RNAi screens

FBgn0004237

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004237

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0004237

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12749

Flygut - An atlas of the Drosophila adult midgut

FBgn0004237

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC004556

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Spliceosome

MIST (protein-protein) - An integrated Molecular Interaction Database

48535

References (187)

The following external sites may use different assemblies or annotations than FlyBase.

-0.40

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