FB2021_01, released Feb 18, 2021

Gene: Dmel\Klp67A

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General Information
Symbol
Dmel\Klp67A
Species
D. melanogaster
Name
Kinesin-like protein at 67A
Annotation Symbol
CG10923
Feature Type
protein_coding_gene
FlyBase ID
FBgn0004379
Gene Model Status
Current
Stock Availability
12 publicly available
Gene Snapshot
Kinesin-like protein at 67A (Klp67A) encodes a microtubule motor protein involved in chromosome congression and mitotic spindle morphogenesis. [Date last reviewed: 2019-07-11]
Other Summaries
Also Known As

DmKlp67A, KLP3

Key Links
Genomic Location
Cytogenetic map
67B2-67B2
Sequence location
3L:9,362,636..9,367,046 [-]
Recombination map

3-29

RefSeq locus
NT_037436 REGION:9362636..9367046
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (32 terms)
Molecular Function (6 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables motor activity
inferred from direct assay
(Pereira et al., 1997)
Terms Based on Predictions or Assertions (6 terms)
CV Term
Evidence
References
enables ATP binding
inferred from electronic annotation with InterPro:IPR001752
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
enables ATP-dependent microtubule motor activity, plus-end-directed
inferred from biological aspect of ancestor with PANTHER:PTN000649974
(assigned by GO_Central )
(Gaudet et al., 2011)
enables ATPase activity
inferred from biological aspect of ancestor with PANTHER:PTN000648413
(assigned by GO_Central )
(Gaudet et al., 2011)
enables microtubule binding
inferred from biological aspect of ancestor with PANTHER:PTN000648413
(assigned by GO_Central )
(Gaudet et al., 2011)
enables microtubule motor activity
inferred from biological aspect of ancestor with PANTHER:PTN000648413
(assigned by GO_Central )
(Gaudet et al., 2011)
enables motor activity
inferred from sequence or structural similarity
(Pereira et al., 1997)
Biological Process (15 terms)
Terms Based on Experimental Evidence (13 terms)
CV Term
Evidence
References
involved_in chromosome segregation
inferred from mutant phenotype
(Wang et al., 2010)
involved_in generative cell mitosis
inferred from mutant phenotype
(Gandhi et al., 2004)
involved_in male meiosis chromosome segregation
inferred from mutant phenotype
(Savoian et al., 2004)
involved_in male meiosis I
inferred from mutant phenotype
(Gandhi et al., 2004)
involved_in male meiotic chromosome movement towards spindle pole
inferred from mutant phenotype
(Savoian et al., 2004)
involved_in meiosis I cytokinesis
inferred from mutant phenotype
(Gandhi et al., 2004)
involved_in microtubule depolymerization
inferred from mutant phenotype
(Goshima and Vale, 2005)
involved_in mitotic centrosome separation
inferred from mutant phenotype
(Gandhi et al., 2004)
involved_in mitotic spindle organization
inferred from mutant phenotype
(Goshima and Vale, 2005)
involved_in sister chromatid biorientation
inferred from mutant phenotype
(Savoian et al., 2004)
involved_in spindle assembly involved in male meiosis I
inferred from mutant phenotype
(Gandhi et al., 2004)
involved_in spindle assembly
inferred from mutant phenotype
(Savoian et al., 2004)
involved_in spindle elongation
inferred from mutant phenotype
(Wang et al., 2010)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
involved_in microtubule depolymerization
inferred from biological aspect of ancestor with PANTHER:PTN000649974
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in microtubule-based movement
inferred from biological aspect of ancestor with PANTHER:PTN000648413
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in mitotic sister chromatid segregation
inferred from biological aspect of ancestor with PANTHER:PTN000649974
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (11 terms)
Terms Based on Experimental Evidence (8 terms)
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Pereira et al., 1997)
located_in kinetochore microtubule
inferred from direct assay
(Goshima and Vale, 2005)
located_in kinetochore
inferred from direct assay
(Savoian and Glover, 2010, Wang et al., 2010, Goshima and Vale, 2005)
part_of microtubule associated complex
inferred from direct assay
(Pereira et al., 1997)
located_in mitotic spindle midzone
inferred from direct assay
(Goshima and Vale, 2005)
located_in mitotic spindle
inferred from direct assay
(Savoian and Glover, 2010)
located_in nucleus
inferred from direct assay
(Wang et al., 2010, Goshima and Vale, 2005, Savoian et al., 2004)
located_in spindle microtubule
inferred from direct assay
(Wang et al., 2010)
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
part_of kinesin complex
inferred from biological aspect of ancestor with PANTHER:PTN000648413
(assigned by GO_Central )
(Gaudet et al., 2011)
traceable author statement
(Pereira et al., 1997)
located_in microtubule
inferred from biological aspect of ancestor with PANTHER:PTN000648413
(assigned by GO_Central )
(Gaudet et al., 2011)
located_in mitotic spindle astral microtubule
inferred from biological aspect of ancestor with PANTHER:PTN000649974
(assigned by GO_Central )
(Gaudet et al., 2011)
located_in mitotic spindle midzone
inferred from biological aspect of ancestor with PANTHER:PTN000649974
(assigned by GO_Central )
(Gaudet et al., 2011)
located_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000649974
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
KINESINS -
Kinesin superfamily proteins (KIFs) are microtubule motor proteins which use the hydrolysis of ATP to drive directional movement along microtubules. KIFs possess a well-conserved 360 residue globular head domain which binds and hydrolyses ATP and interacts with microtubules. Many KIFs homodimerize via coiled-coil interactions in the stalk region. KIFs bind cargo through their variable tail regions and are involved in transporting organelles, protein complexes, mRNAs and the movement of spindles and chromosomes during cell division. (Adapted from FBrf0219884).
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\Klp67A for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.46

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Klp67A-RA
FBtr0076499
NM_079268
2899
814
Klp67A-RB
FBtr0333886
NM_001274695
2991
740
Klp67A-RC
FBtr0346530
NM_001300070
2994
814
Additional Transcript Data and Comments
Reported size (kB)

3.0, 2.8 (northern blot)

(Stewart et al., 1991)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Klp67A-PA
FBpp0076227
92.3
814
9.67
NP_523992
AAF50296
Klp67A-PB
FBpp0306018
84.4
740
8.74
NP_001261624
AGB94319
Klp67A-PC
FBpp0312148
92.3
814
9.67
NP_001286999
AHN58024
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

814 aa isoforms: Klp67A-PA, Klp67A-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Klp67A using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism
(Fisher et al., 2012)
embryonic stage 7 -- 8
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 9 -- 10
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 11 -- 12
central brain primordium
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
embryonic central brain neuron
(Fisher et al., 2012)
embryonic central brain surface glia
(Fisher et al., 2012)
embryonic head sensory system
(Fisher et al., 2012)
embryonic/larval lymph gland
(Fisher et al., 2012)
embryonic/larval nervous system
(Fisher et al., 2012)
gonad
(Fisher et al., 2012)
lateral cord neuron
(Fisher et al., 2012)
lateral cord surface glial cell
(Fisher et al., 2012)
ventral nerve cord
(Fisher et al., 2012)
wandering third instar larval stage
wing pouch | restricted
(Molnar et al., 2012)
wing hinge primordium | restricted
(Molnar et al., 2012)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage & larval stage & pupal stage
(Stewart et al., 1991)
adult stage | female
ovary
(Stewart et al., 1991)
adult stage | male
testis
(Stewart et al., 1991)
Additional Descriptive Data

Klp67A transcripts are expressed throughout development. In adults, they are detected only in the gonads.

(Stewart et al., 1991)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Pereira et al., 1997)
located_in kinetochore microtubule
inferred from direct assay
(Goshima and Vale, 2005)
located_in kinetochore
inferred from direct assay
(Savoian and Glover, 2010, Wang et al., 2010, Goshima and Vale, 2005)
part_of microtubule associated complex
inferred from direct assay
(Pereira et al., 1997)
located_in mitotic spindle midzone
inferred from direct assay
(Goshima and Vale, 2005)
located_in mitotic spindle
inferred from direct assay
(Savoian and Glover, 2010)
located_in nucleus
inferred from direct assay
(Wang et al., 2010, Goshima and Vale, 2005, Savoian et al., 2004)
located_in spindle microtubule
inferred from direct assay
(Wang et al., 2010)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Klp67A in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 5 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 13 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Klp67A
Transgenic constructs containing regulatory region of Klp67A
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation
Inferred from location
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
partially lethal - majority die | recessive
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
female semi-sterile (with Df(3L)29A6)
fertile (with Klp67A322b24), with Df(3L)29A6
fertile (with Klp67AUbi-p63E.GFP), with Df(3L)29A6
male sterile (with Df(3L)29A6)
Other Phenotypes
Allele
abnormal cytokinesis | male (with Df(3L)29A6)
abnormal meiotic cell cycle | male
abnormal meiotic cell cycle | male, with Scer\GAL4- :
abnormal meiotic cell cycle | male (with Df(3L)29A6)
abnormal mitotic cell cycle (with Df(3L)29A6)
Phenotype manifest in
Allele
centrosome | blastoderm stage | maternal effect (with Df(3L)29A6)
meiosis I | male (with Df(3L)29A6)
meiosis II | male (with Df(3L)29A6)
meiotic anaphase II & condensed nuclear chromosome | male (with Df(3L)29A6)
meiotic anaphase II & condensed nuclear chromosome | male (with Df(3L)29A6), with Scer\GAL4- :
mitotic anaphase B | blastoderm stage | maternal effect (with Df(3L)29A6)
mitotic metaphase | blastoderm stage | maternal effect (with Df(3L)29A6)
mitotic prometaphase & nuclear chromosome | male (with Df(3L)29A6)
mitotic prometaphase & nuclear chromosome | male (with Df(3L)29A6), with Scer\GAL4- :
spermatid & Nebenkern (with Df(3L)29A6)
spermatid & nucleus (with Df(3L)29A6)
spermatocyte & aster | ectopic (with Df(3L)29A6)
spermatocyte & astral microtubule (with Df(3L)29A6)
spermatocyte & centrosome | supernumerary (with Df(3L)29A6)
spermatocyte & condensed nuclear chromosome (with Df(3L)29A6)
spermatocyte & contractile ring (with Df(3L)29A6)
spermatocyte & nuclear chromosome (with Df(3L)29A6)
spermatocyte & nuclear chromosome (with Df(3L)29A6), with Scer\GAL4- :
spermatocyte & spindle | supernumerary (with Df(3L)29A6)
spindle | blastoderm stage | maternal effect (with Df(3L)29A6)
spindle | embryonic stage 4 (with Df(3L)29A6)
spindle microtubule | blastoderm stage | maternal effect (with Df(3L)29A6)
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (6)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\KIF18A
12 of 15
Yes
Yes
Hsap\KIF18B
8 of 15
No
Yes
Hsap\KIF19
3 of 15
No
No
Hsap\CENPE
1 of 15
No
No
Hsap\KIF6
1 of 15
No
Yes
Hsap\KIF22
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (7)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Kif18a
11 of 15
Yes
Yes
Mmus\Kif18b
8 of 15
No
Yes
Mmus\Kif19a
4 of 15
No
No
Mmus\Cenpe
1 of 15
No
No
Mmus\Gm4869
1 of 15
No
No
Mmus\Kif6
1 of 15
No
Yes
Mmus\Kif22
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (7)
Rnor\Kif18a
9 of 13
Yes
Yes
Rnor\Kif18b
8 of 13
No
Yes
Rnor\Kif19
2 of 13
No
No
Rnor\Cenpe
1 of 13
No
No
Rnor\Kif6
1 of 13
No
Yes
Rnor\Kif22
1 of 13
No
Yes
Rnor\LOC679924
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (6)
Xtro\kif18a
10 of 12
Yes
Yes
Xtro\kif18b
6 of 12
No
Yes
Xtro\kif19
2 of 12
No
No
Xtro\cenpe
1 of 12
No
No
Xtro\kif22
1 of 12
No
Yes
Xtro\LOC100494536
1 of 12
No
No
Danio rerio (Zebrafish) (5)
Drer\kif18a
12 of 15
Yes
Yes
Drer\kif19
3 of 15
No
No
Drer\si:dkey-26i13.8
3 of 15
No
No
Drer\cenpe
1 of 15
No
No
Drer\kif22
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\klp-13
3 of 15
Yes
No
Arabidopsis thaliana (thale-cress) (2)
Atha\AT1G18550
5 of 9
Yes
Yes
Atha\AT3G49650
4 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (2)
Scer\KIP3
10 of 15
Yes
Yes
Scer\KIP2
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (2)
Spom\klp5
8 of 12
Yes
Yes
Spom\klp6
8 of 12
Yes
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091902SO )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00003834
Drosophila simulans
Dsim\GD12936
Drosophila sechellia
Dsec\GM24884
Drosophila erecta
Dere\GG14050
Drosophila yakuba
Dyak\GE21253
Drosophila ananassae
Dana\GF23742
Drosophila pseudoobscura pseudoobscura
Dpse\GA10646
Drosophila persimilis
Dper\GL22636
Drosophila willistoni
Dwil\GK16568
Drosophila virilis
Dvir\GJ13594
Drosophila mojavensis
Dmoj\GI11399
Drosophila grimshawi
Dgri\GH16987
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091501LG )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17007862
Glossina morsitans
Tsetse fly
Gmor\GMOY007810
Glossina morsitans
Tsetse fly
Gmor\GMOY007054
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_05926
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_05917
Mayetiola destructor
Hessian fly
Mdes\Mdes006266
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL009227
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL013401
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL015012
Anopheles darlingi
American malaria mosquito
Adar\ADAC004682
Anopheles gambiae
Malaria mosquito
Agam\AGAP006803
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ004544
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W02R9 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA010931
Danaus plexippus
Monarch butterfly
Dple\DPOGS209928-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL017394
Apis florea
Little honeybee
Aflo\2446442260
Apis mellifera
Western honey bee
Amel\GB45830
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP22453
Bombus terrestris
Buff-tailed bumblebee
Bter\15214089
Linepithema humile
Argentine ant
Lhum\LH19284
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:00125a
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG014906
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_06157
Tribolium castaneum
Red flour beetle
Tcas\TC002763
Rhodnius prolixus
Kissing bug
Rpro\RPRC001407
Cimex lectularius
Bed bug
Clec\CLEC000400
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI001356
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_13914
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X014J )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Tetranychus urticae
Two-spotted spider mite
Turt\tetur21g02500
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_129226
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0438 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Kif18
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Kif18AL3
Gallus gallus
Domestic chicken
Ggal\KIF18A
Gallus gallus
Domestic chicken
Ggal\KIF18B
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (26)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Kif19A
6 of 10
cana
3 of 10
cmet
3 of 10
CG32318
2 of 10
cos
2 of 10
Khc-73
2 of 10
Khc
2 of 10
Kif3C
2 of 10
Klp3A
2 of 10
Klp10A
2 of 10
Klp31E
2 of 10
Klp54D
2 of 10
Klp59C
2 of 10
Klp59D
2 of 10
Klp61F
2 of 10
Klp64D
2 of 10
Klp68D
2 of 10
Klp98A
2 of 10
ncd
2 of 10
neb
2 of 10
nod
2 of 10
pav
2 of 10
sub
2 of 10
unc-104
2 of 10
CG5004
1 of 10
CG14535
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    KIF18A; kinesin family member 18A
    12 of 15
    611271
        KIF18B; kinesin family member 18B
        8 of 15
        614570
            KIF19; kinesin family member 19
            3 of 15
                Functional Complementation Data
                Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                Interactions
                Summary of Physical Interactions
                esyN Network Diagram
                Interactions Browser
                Summary of Genetic Interactions
                esyN Network Diagram
                Starting gene(s)
                Interaction type
                Interacting gene(s)
                Reference
                Starting gene(s)
                Interaction type
                Interacting gene(s)
                Reference
                External Data
                Linkouts
                DroID - A comprehensive database of gene and protein interactions.
                InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
                MIST (genetic) - An integrated Molecular Interaction Database
                Pathways
                Signaling Pathways (FlyBase)
                Metabolic Pathways
                External Data
                Linkouts
                Genomic Location and Detailed Mapping Data
                Chromosome (arm)
                3L
                Recombination map

                3-29

                Cytogenetic map
                67B2-67B2
                Sequence location
                3L:9,362,636..9,367,046 [-]
                FlyBase Computed Cytological Location
                Cytogenetic map
                Evidence for location
                67B2-67B2
                Limits computationally determined from genome sequence between P{PZ}l(3)0162901629&P{PZ}mRpL1210534 and P{EP}Hsp26EP3336&P{EP}Hsp26EP3315
                Experimentally Determined Cytological Location
                Cytogenetic map
                Notes
                References
                67B1-67B5
                (determined by in situ hybridisation)
                (Deak et al., 1997)
                Experimentally Determined Recombination Data
                Location

                3-29

                (FlyBase, 2016)

                3-23.0

                (Comeron et al., 2012)
                Left of (cM)
                Right of (cM)
                Notes
                Stocks and Reagents
                Stocks (12)
                Genomic Clones (22)
                cDNA Clones (22)
                 

                Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

                cDNA clones, fully sequenced
                BDGP DGC clones
                Other clones
                Drosophila Genomics Resource Center cDNA clones

                For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                cDNA Clones, End Sequenced (ESTs)
                BDGP DGC clones
                Other clones
                RNAi and Array Information
                Linkouts
                DRSC - Results frm RNAi screens
                GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                Antibody Information
                Laboratory Generated Antibodies
                 

                polyclonal

                (Wang et al., 2010)
                Commercially Available Antibodies
                 
                Other Information
                Relationship to Other Genes
                Source for database identify of

                Source for identity of: Klp67A CG10923

                (Milshina, 1999.11)
                Source for database merge of

                Source for merge of: Klp67A l(3)S042705

                (Savoian et al., 2004)

                Source for merge of: Klp67A anon-WO0140519.224 anon-WO0172774.120

                (FlyBase, 1996-)
                Additional comments

                Source for merge of Klp67A anon-WO0140519.224 anon-WO0172774.120 was sequence comparison ( date:051113 ).

                (FlyBase, 1996-)
                Other Comments

                S2 cells treated with RNAi against Klp10A show increased poleward flux rates of tubulin subunits in mitotic spindles and show suppressed α-tubulin turnover at the spindle equator but not at the poles. The chromatid-to-pole velocity during anaphase is increased in these cells. Pole-to-pole spindle lengths are significantly increased in the RNAi treated cells compared to controls.

                (Buster et al., 2007)

                RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle and an aberrantly long spindle that is monastral and bipolar when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

                (Goshima et al., 2007)

                S2 cells transfected with dsRNA made from templates generated with primers directed against this gene show an expansion of the metaphase spindle. Sometimes RNAi of this gene show centrosome detachment from the kinetochore microtubules.

                (Goshima et al., 2005)

                RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in DL2 cells: cells become arrested in metaphase and contain abnormally elongated, curved spindles which cause the entire cell to assume an elongated shape and exclude mitochondria from the cell poles. Additionally, metaphase chromosomes show an abnormal configuration.

                (Gandhi et al., 2004)

                The majority of post-meiotic Klp67A mutant spermatids contain two nuclei, which vary in size. During meiosis I, mutant primary spermatocytes have an increased number of microtubules throughout the cell and lack a distinct central spindle. Additionally, the k-fibres that link the chromosomes to the spindle are bent and wavy, instead of being straight, as in wild type.

                (Savoian et al., 2004)

                dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

                (Kiger et al., 2003)

                Klp67A is a mitotic motor that may have a unique role of positioning mitochondria near the spindle.

                (Pereira et al., 1997)

                Klp67A has been cloned, partially sequenced and its expression pattern analysed.

                (Stewart et al., 1991)
                Origin and Etymology
                Discoverer
                Etymology
                Identification
                External Crossreferences and Linkouts ( 46 )
                Sequence Crossreferences
                NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                Other crossreferences
                BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                Flygut - An atlas of the Drosophila adult midgut
                GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                InterPro - A database of protein families, domains and functional sites
                KEGG Genes - Molecular building blocks of life in the genomic space.
                modMine - A data warehouse for the modENCODE project
                Linkouts
                DPiM - Drosophila Protein interaction map
                DroID - A comprehensive database of gene and protein interactions.
                DRSC - Results frm RNAi screens
                FLIGHT - Cell culture data for RNAi and other high-throughput technologies
                FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                FlyMine - An integrated database for Drosophila genomics
                InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
                MIST (genetic) - An integrated Molecular Interaction Database
                Synonyms and Secondary IDs (24)
                Reported As
                Symbol Synonym
                0427/05
                (Deak et al., 1997)
                CG10923
                (Çiçek et al., 2016, Cui et al., 2013, Moutinho-Pereira et al., 2013, Schertel et al., 2013, Ni et al., 2011.7.19, Keleman et al., 2009.8.5, Perkins et al., 2009, Hughes et al., 2008, Goshima et al., 2007, Goldstein and Gunawardena, 2000)
                DmKlp67A
                (Hirokawa, 2012, Dagenbach and Endow, 2004, Dagenbach and Endow, 2004)
                KIF 18A
                (Miki et al., 2001)
                KIF18A/B
                (Hirokawa, 2012.11.20)
                KLP3
                (Pereira et al., 1991, Stewart et al., 1991)
                KLP67A
                (Giansanti et al., 2012, Fernandez et al., 2009, Buster et al., 2007, Vendra et al., 2007, Miki et al., 2005, Gandhi et al., 2004, Kwon and Scholey, 2004, Lawrence et al., 2002, Hirokawa et al., 1998, Pereira and Karr, 1998, Pereira et al., 1997)
                KRP67A
                (Lane and Allan, 1998)
                Kin-8
                (Pandey et al., 2007)
                Klp3
                Klp67
                (Damasco et al., 2011)
                Klp67A
                (Parker et al., 2020, Witte et al., 2020, Fingerhut et al., 2019, Jia et al., 2019, Rotelli et al., 2019, Yee et al., 2019, Tubman et al., 2018, Bougé and Parmentier, 2016, Tao et al., 2016, Liang et al., 2014, Cui et al., 2013, Moutinho-Pereira et al., 2013, Schertel et al., 2013, Giansanti et al., 2012, Hirokawa, 2012.11.20, Korenjak et al., 2012, Savoian and Glover, 2010, Fisher et al., 2008, Somma et al., 2008, Georlette et al., 2007, Goshima et al., 2007, Pandey et al., 2007, Laycock et al., 2006, D'Avino et al., 2005, Gatt et al., 2005, Goshima and Vale, 2005, Miki et al., 2001)
                anon-WO0140519.224
                anon-WO0172774.120
                klp67
                (Blanchard et al., 2014)
                klp67A
                (Giansanti et al., 2012, Savoian and Glover, 2010, Wang et al., 2010)
                l(3)S042705
                Name Synonyms
                Kinesin 8
                (Pandey et al., 2007)
                Kinesin-8
                (Savoian and Glover, 2010)
                Kinesin-like protein at 67A
                (Hirokawa, 2012.11.20)
                Kinesin8A
                (Kollmar, 2015.9.15)
                kinesin like protein 67a
                (Pereira, 1997.2.10)
                kinesin-8
                (Fernandez et al., 2009)
                lethal (3) S042705
                Secondary FlyBase IDs
                • FBgn0044647
                • FBgn0046473
                • FBgn0060914
                Datasets (0)
                Study focus (0)
                Experimental Role
                Project
                Project Type
                Title
                References (96)