A DEAD-box helicase, part of a ribonuclear protein complex, that restricts translation of oocyte-localizing RNAs - in neurons Me31B acts to promote translational repression and/or mRNA degradation in response to miRNAs
Gene model reviewed during 5.46
None of the polypeptides share 100% sequence identity.
Conserved component of different types of multiprotein ribonucleoprotein complexes (RNPs) that form distinct germ granules (P-body, nuage, sponge body or polar granules) and P-body-like neuronal RNPs (PubMed:11546740, PubMed:16256742, PubMed:18765641, PubMed:18590813, PubMed:19285948, PubMed:28388438). Consequently it interacts with a wide variety of proteins, some of which appear to be common interactive partners in almost all RNPs types i.e. cup and tral, whereas other interactions are specific to a germ granule/RNP (PubMed:28945271). Core functional components in me31B-containing RNPs include RNA regulatory proteins (such as translational repressor, RNA-decapping and exonuclease proteins), RNA localization proteins and additional proteins depending on the biological context of the RNPs (PubMed:17178403, PubMed:28945271). In the P-body RNPs, interacts with at least the translation repressor proteins tral, cup and Edc3, and the mRNA localization factor yps (PubMed:16256742, PubMed:18765641, PubMed:18590813, PubMed:19285948). Interaction with tral or Edc3 is required for translation repression and possibly RNA decapping; binding to tral and Edc3 is mutually exclusive (PubMed:18765641, PubMed:19285948). In the nuage and germ plasm polar granule RNPs, interacts with at least tral, cup, and additional proteins required for assembly and function of the germ granules such as tud, vas and aub (PubMed:18765641, PubMed:18590813, PubMed:19285948, PubMed:28945271). Interacts (when dimethylated on Arg residues) with tud; interaction is RNA-independent (PubMed:28945271). Component of the osk RNP complex, which is composed of at least me31B, exu, yps, aret/bruno, cup, and the mRNA of osk (PubMed:10662770). Component of the nos RNP complex, which is composed of at least smg, cup, tral, me31B, the CCR4-NOT complex members Rga/NOT2 and Caf1-55, and the mRNA of nos (PubMed:21081899). Interacts with tral and piRNA pathway components papi and AGO3; promotes interaction between nuage RNPs and the piRNA-mediated transposon silencing (PubMed:21447556). Forms a RNP containing at least me31B, eIF4E1, cup, tral and pAbp; this interaction is required for the translational silencing of maternal mRNAs during the maternal-to-zygotic transition (PubMed:28875934). In the sponge body, forms a RNP containing at least me31B, exu, yps and the mRNA of osk; interactions with exu and yps are RNA dependent (PubMed:11546740). Component of a neuronal RNP, at least composed of me31B, tral and Fmr1 (PubMed:17178403). Component of the Atx2-Not1 repressor complex, composed of at least me31B, Atx2, tyf and pAbp (PubMed:28388438). Interacts (via the C-terminus) with Atx2, tyf, pAbp and Lsm12a (PubMed:28388438). Interacts (via RecA-like domain 2) with 4EHP-GYF2 complex member Gyf (via the me31B binding motif) (PubMed:31114929, PubMed:31439631). Interacts with 4E-T, Edc3 and Patr-1 (PubMed:31439631).
Symmetrically dimethylated on arginine residues.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\me31B using the Feature Mapper tool.
me31B is ubiquitously expressed in the adult brain. Closer examination in antennal lobes shows punctate expression in cell bodies and in the synaptic neuropil. Expression is observed in projection neurons, local interneurons, and mushroom body Kenyon cells. me31B is also strongly expressed in glial cells which send processes into the antennal lobe.
GBrowse - Visual display of RNA-Seq signalsView Dmel\me31B in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: me31B l(2)k06607