αPS1, αPS1, PS1, PS1α, αPS1 integrin
Stop-codon suppression (UAA) postulated; FBrf0216884.
Gene model reviewed during 5.43
Shares 5' UTR with upstream gene.
Gene model reviewed during 5.44
Gene model reviewed during 5.50
5.4 (northern blot)
None of the polypeptides share 100% sequence identity.
1146 (aa); 128 (kD predicted)
Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-PS1 associates with beta-PS.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mew using the Feature Mapper tool.
Transcript was detected in stage 11 salivary gland primordia coincident with salivary gland internalization. Expression continued in all cells through stage 12 with high expression in invaginating cells. Transcritp was no longer detectable at stage 14. mew is also expressed in the invaginating tracheal pits.
mew transcripts are first detected at the cellular blastoderm stage in the cells of the presumptive ectoderm. During gastrulation, the transcript is apparent over the entire ectoderm with increased levels in the dorsal region. In stage 8, high transcript levels are observed in the ectoderm and in the anterior and posterior midgut primordia. By stage 11, a strong segmentally repeated pattern has been established in the ectoderm and the CNS. The regions of high concentration are correlated with segment boundaries. Strong mew expression is also detected in the dorsal compartment of the wing disc as well as in adepithelial cells and on the peripodial membrane.
The α1 integrin, mew, strongly accumulates on the basal membrane of esg-positive cells contacting ECM, but is barely detected in any other regions along the interface between the muscle cells and the differentiated epithelial cells.
In stage 15 embryos, before the leading processes of the cardioblasts make contact across the midline, mew is localised primarily on the leading edge of the migrating cardioblasts, and less so on the trailing edge. This preponderantly apical distribution was not significantly altered once the heart lumen was formed at stage 17.
mew staining is observed along lateral, apical, and basal follicle cell membranes through stage 10A. Apical staining peaks in stage 9 and 10A columnal follicle cells overlying the oocyte while downregulating in the most posterior follicle cells. Expression is also seen in border cells at intercellular junctions. After stage 10A, the remaining follicle cell populations begin down-regulating apically localized mys while maintaining lateral and basal localization during dorsal appendage morphogenesis. Staining is observed from stage 9 in dorsal appendage forming follicle cells.
GBrowse - Visual display of RNA-Seq signalsView Dmel\mew in GBrowse 2
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
One of 42 Drosophila genes identified as being most likely to reveal molecular and cellular mechanisms of nervous system development or plasticity relevant to human Mental Retardation disorders.
Identified as a gene with significant level of mRNA cycling as assessed by expression analysis using high density oligonucleotide arrays with probe generated from adult heads harvested over six time points over the course of a day.
The functional significance of the cytoplasmic domains of the if, mew and mys subunits of the Position Specific (PS) integrin family are studied by analysing the relationship between the cytoplasmic domain structure and function in the context of a developing organism. The cytoplasmic tail of mew is not required for function in the wing and in the retina.
The ability of two different integrin α subunits (encoded by mew and if) to substitute for each other during embryonic development has been studied. The two α subunits encoded by mew and if are not equivalent and have distinct functions which reside in the extracellular domains.
Clonal analysis demonstrates that the different integrins, mew and if, are required on opposite wing epithelia. An early integrin-dependent process, not obviously required for prepupal adhesion, is essential to permit subsequent wing morphogenesis.
Phenotypic analysis of mew, if and mys embryos suggests multiple roles for PS integrins in the adhesion of cells and in the formation, organization and migration of embryonic tissues. Although the proteins are often expressed in adjacent embryonic tissues, this distribution does not necessarily reflect equivalent requirements. The complete loss of both α subunits, encoded by mew and if, does not produce all the phenotypes observed in embryos lacking the mys encoded β subunit.
The chosen gene symbol, "mew", will commemorate the name of the late Michael E. Wilcox, who made many important contributions to this field.