Dtrk, Dtkr
CCK-4 family of 'dead' receptor tyrosine kinases, Ig-domains - required for lamina-specific targeting of R1-R6 axons - associates with Plexin, the receptor for Semaphorin ligand
Please see the JBrowse view of Dmel\otk for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.49
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.55
5.7 (northern blot)
None of the polypeptides share 100% sequence identity.
1033 (aa); 160 (kD observed)
Interacts with plexA; component of a receptor complex that mediates the repulsive signaling in response to Semaphorin ligands.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\otk using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: cyclical, peak ZT1-4
Expressed cyclically in the adult fat body.
Two major peaks of otk expression are seen on northern blots. They occur in early-mid embryos and in early pupae.
otk protein expression is first detected at embryonic stage 7 in the anterior midgut primordium, cephalic furrow, and along the germ band. At stage 11, expression is observed in 15 stripes corresponding to parasegments 1-15, including external epidermal cells and internalized neuroblasts of the CNS. Within each parasegment, staining is in the posterior compartment. On the epidermis, staining comprises ventral and lateral regions but does not include the midline. Later, staining is observed in the anterior compartment. At the beginni g of germ band retraction, expression is observed in segmentally repeated patches of neuroepithelial cells and more strongly in underlying neuronal cells. It is also detected transiently in the maxillary bud. In stage 13, protein disappears from the epithelium and condenses in the ventral nerve cord. It is also observed in three discontinuous gut areas including the anterior midgut, posterior midgut and hindgut where it is restricted to the visceral mesoderm. At later stages, expression is barely detectable in mesodermal tissues but is strong in the ventral cord. Protein is present throughou the CNS on neuronal cell bodies and their axons. Staining is stronger in anterior commissures than posterior commissures. Protein is also detected on axons leaving the CNS through the segmental and intersegmental nerves, in neurons of a subset of sensory organs of the PNS, and on fasciculating axons growing toward muscle targets.
JBrowse - Visual display of RNA-Seq signals
View Dmel\otk in JBrowse2-65
2-66.7
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Phylogenetic analysis of the PTK family.
The otk gene product promotes cell adhesion in a homophilic calcium- dependent manner and this adhesion process specifically activates its kinase behaviour.
Identification: By sequence similarity to human trk (receptor tyrosine kinase) cDNA probes.
otk has been identified, sequence and its expression pattern characterised.
