RNA polymerase II, RpII15, PolII, Pol II, RNAP II
Gene model reviewed during 5.53
Gene model reviewed during 5.41
Gene model reviewed during 5.56
1.2, 0.85 (northern blot)
129 (aa); 15.1 (kD)
Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Polr2I using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Polr2I in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Polr2I RpII15
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly long spindles with misaligned chromosomes when assayed in S2 cells in the presence of Cdc27 dsRNA. This phenotype cannot be observed when the screen is performed without Cdc27 dsRNA.
Used in a phylogenetic analysis, the tree in inferred by parsimony method from RpoB sequences, or homologous, multiple alignment.
Designation as "RpII215" was in error.
Isolation of the coding sequence and immunoblotting experiments demonstrate the RpII15 gene codes for a small subunit of RNA polymerase II.
The technique of paramagnetic particle-mediated selection of terminated run-on transcripts was used to examine RNA polymerase II pausing and 5' cap formation at high resolution on the heat shock genes Hsp70A, Hsp70B, Hsp26 and Hsp27. Results demonstrate that polymerases pause over a narrow region of each heat shock gene, not at a defined point. 5' capping occurs over a region coincidental with that of pausing.
Protein complexes of RNA polymerase II localise to major developmental puffs and heat shock puffs.
RpII15 is an essential gene required maternally for embryogenesis.
Named 'Polr2I' after the human ortholog.