FlyBase Gene Report: Dmel\Eip78C

General Information

Symbol

Dmel\Eip78C

Species

D. melanogaster

Name

Ecdysone-induced protein 78C

Annotation Symbol

CG18023

Feature Type

protein_coding_gene

FlyBase ID

FBgn0004865

Gene Model Status

Current

Stock Availability

32 publicly available

Gene Summary

Induces the early late puff 78C which triggers puparium formation and development. (UniProt, P45447)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

E78, E78B, E78A, DR-78, nuclear hormone receptor

Key Links

Genomic Location

Cytogenetic map

78C2-78C3

Sequence location

3L:21,226,609..21,266,503 [+]

Recombination map

3-47

RefSeq locus

NT_037436 REGION:21226609..21266503

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (5 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables DNA-binding transcription factor activity

inferred from electronic annotation with InterPro:IPR001628

(InterPro Project Members, 2004-)

enables sequence-specific DNA binding

inferred from electronic annotation with InterPro:IPR001628

(InterPro Project Members, 2004-)

enables zinc ion binding

inferred from electronic annotation with InterPro:IPR001628, InterPro:IPR013088

(InterPro Project Members, 2004-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in negative regulation of DNA-templated transcription

inferred from sequence or structural similarity with UniProtKB:Q14995

(Gene Ontology Curators, 2002-)

involved_in regulation of DNA-templated transcription

inferred from electronic annotation with InterPro:IPR001628, InterPro:IPR013088

(InterPro Project Members, 2004-)

Cellular Component (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

NUCLEAR RECEPTOR (LIGAND-DEPENDENT) TRANSCRIPTION FACTORS

Protein Family (UniProt)

Belongs to the nuclear hormone receptor family. NR1 subfamily. (P45447)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

NUCLEAR RECEPTOR (LIGAND-DEPENDENT) TRANSCRIPTION FACTORS -: Nuclear receptors (NR) are C4 zinc finger ligand-dependent DNA-binding transcription factors. Members of the NR superfamily are defined by the presence of a highly conserved DNA-binding domain and a less conserved C-terminal ligand-binding domain. (Adapted from FBrf0184203).

Protein Function (UniProtKB)

Induces the early late puff 78C which triggers puparium formation and development.

(UniProt, P45447)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Eip78C for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P45447)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.40

Unconventional translation start (CUG) postulated; FBrf0064719

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Eip78C-RA

FBtr0078306

NM_079471

5223

862

Eip78C-RB

FBtr0078307

NM_168892

3832

392

Eip78C-RD

FBtr0306157

NM_001259933

4192

862

Eip78C-RE

FBtr0306158

NM_001259934

3541

862

Additional Transcript Data and Comments

Reported size (kB)

4.8, 4.3, 4.1, 4.0, 3.5, 3.2, 2.6 (northern blot)

(Stone and Thummel, 1993)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Eip78C-PA

FBpp0077963

94.7

862

5.14

FBpp0077964

43.2

392

6.51

NP_730609

AAN12161

Eip78C-PD

FBpp0297287

94.7

862

5.14

FBpp0297288

94.7

862

5.14

P45447

NP_001246863

AFH04534

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

862 aa isoforms: Eip78C-PA, Eip78C-PD, Eip78C-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

864, 390 (aa); 95.9, 43.8 (kD)

(Stone and Thummel, 1993)

Comments

Translation initiates at a CUG codon. This protein lacks a DNA-binding domain but shares the ligand-binding/dimerization domain with the other major protein product of the Eip78C gene, Eip78C⁺P864.

(Stone and Thummel, 1993)

One of a couple of protein products.

(FlyBase, 1992-)

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Eip78C using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.44

Transcript Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

second instar larval stage

Comment: reference states 32-48 hr post-hatching

(Stone and Thummel, 1993)

pharate adult stage P8

Comment: reference states 48-56 hr APF

(Stone and Thummel, 1993)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

second instar larval stage

Comment: reference states 32-48 hr post-hatching

(Stone and Thummel, 1993)

wandering third instar larval stage -- prepupal stage P4

organism

Comment: -3-12 hr APF

(Stone and Thummel, 1993)

third instar larval stage L2 -- prepupal stage P4

organism

Comment: -3-12 hr APF

(Stone and Thummel, 1993)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: PBac{Eip78C-GFP.FLAG}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female oogenesis stage S1 -- S10

nurse cell

• nucleus

(Ables et al., 2015)

adult stage | female oogenesis stage S8 -- S12

main body follicle cell

• nucleus

(Ables et al., 2015)

border follicle cell

• nucleus

(Ables et al., 2015)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Eip78C in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Eip78C

View exonic expression by tissue for Dmel\Eip78C

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40345/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004865

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004865

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004865

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

Flygut - An atlas of the Drosophila adult midgut

FBgn0004865

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 43 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 18 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Eip78C

Transgenic constructs containing regulatory region of Eip78C

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(2L)ppl⁰⁶⁹¹³

(Zinke et al., 1999)

Df(3L)ME1325

(Russell et al., 1996, Carpenter, 1994)

Df(3L)Pc-101

(Russell et al., 1996)

Inferred from location ( 3 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Bx-MS1096

Eip78C^{UAS.ORF.GW.Tag:HA}

lethal | cold sensitive, with Scer\GAL4^Bx-MS1096

Eip78C^{UAS.ORF.GW.Tag:HA}

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Eip78C^GD4135

partially lethal - majority die | nutrition conditional (with Df(3L)BSC419)

Eip78C^Δ31

viable

viable, with Scer\GAL4^tin.CΔ4

Eip78C^GD4135

viable (with Eip78C^Δ18)

Eip78C^Δ37

viable (with Eip78C^Δ37)

Eip78C^Δ18

Sterility

Allele

female fertile

Eip78C^A464.2M3

female semi-sterile (with Df(3L)BSC419)

Eip78C^Δ31

female sterile | recessive

Eip78C^Δ31

fertile

Eip78C^ME534

fertile (with Eip78C^Δ18)

Eip78C^Δ37

fertile (with Eip78C^Δ37)

Eip78C^Δ18

male fertile

Eip78C^A464.2M3

Other Phenotypes

Allele

decreased fecundity | female (with Df(3L)BSC419)

Eip78C^Δ31

increased cell death | adult stage, with Scer\GAL4^{VP16.nanos.UTR}

increased cell death | adult stage (with Df(3L)BSC419)

Eip78C^Δ31

increased cell death | adult stage | germline clone

Eip78C^Δ31

increased mortality during development | parental effect, with Scer\GAL4^esg.PU

Eip78C^JF02258

majority die during embryonic stage | maternal effect (with Df(3L)BSC419)

Eip78C^Δ31

some die during embryonic stage | maternal effect

Eip78C^8h

some die during pupal stage, with Scer\GAL4^pnr-MD237

Eip78C^GD4135

visible, with Scer\GAL4^ey.PH

Eip78C^EP3468

visible, with Scer\GAL4^GMR.PF

Eip78C^EP521

visible | female limited, with Scer\GAL4^Bx-MS1096

Eip78C^GD4135

Phenotype manifest in

Allele

dorsal appendage

eye, with Scer\GAL4^ey.PH

Eip78C^EP3468

eye, with Scer\GAL4^GMR.PF

Eip78C^EP521

female germline cyst, with Scer\GAL4^{VP16.nanos.UTR}

female germline cyst (with Df(3L)BSC419)

Eip78C^Δ31

female germline cyst | germline clone

Eip78C^Δ31

germarium, with Scer\GAL4^bab1-Gal4

germarium (with Df(3L)BSC419)

Eip78C^Δ31

germarium cap cell, with Scer\GAL4^bab1-Gal4

germarium cap cell (with Df(3L)BSC419)

Eip78C^Δ31

germline stem cell, with Scer\GAL4^bab1-Gal4

germline stem cell | adult stage (with Df(3L)BSC419)

Eip78C^Δ31

ommatidium, with Scer\GAL4^ey.PH

Eip78C^EP3468

wing | female limited, with Scer\GAL4^Bx-MS1096

Eip78C^GD4135

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (46)

Hsap\NR1D2

5 of 14

Yes

Hsap\NR1D1

4 of 14

Yes

4 of 14

4 of 14

4 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (37)

Rnor\Nr1d1

4 of 14

Yes

Rnor\Nr1d2

4 of 14

Yes

Rnor\Rora

4 of 14

Yes

Rnor\Rorb

4 of 14

Yes

Rnor\Rorc

4 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (38)

Mmus\Nr1d2

5 of 14

Yes

Mmus\Nr1d1

4 of 14

Yes

4 of 14

4 of 14

4 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (32)

Xtro\banf1

3 of 13

Yes

Xtro\nr1d1

3 of 13

Yes

Xtro\rora

3 of 13

Yes

Xtro\rorb.2

3 of 13

Yes

Xtro\ubxn6

3 of 13

Yes

Xtro\cep55

2 of 13

Xtro\rorc

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (58)

Drer\nr1d2a

5 of 14

Yes

Drer\nr1d2b

5 of 14

Yes

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

3 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (74)

Cele\sex-1

5 of 14

Yes

4 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\nhr-69

1 of 14

Cele\nhr-71

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (18)

Agam\AgaP_AGAP006571

8 of 12

Yes

Agam\AgaP_AGAP009002

3 of 12

Agam\AgaP_AGAP012222

2 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Eip78C. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (17)

Hr3

4 of 13

EcR

2 of 13

Eip75B

2 of 13

dsf

1 of 13

ERR

1 of 13

Hnf4

1 of 13

Hr4

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

kni

1 of 13

knrl

1 of 13

svp

1 of 13

tll

1 of 13

usp

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

Eip78C^JF02258

model of amyotrophic lateral sclerosis

CEA with Hsap\TARDBP^{G298S.UAS.YFP}

(Joardar et al., 2015)

CEA with Hsap\TARDBP^UAS.YFP.cEa

(Joardar et al., 2015)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Eip78C^JF02258

ameliorates C9orf72 frontotemporal dementia and/or amyotrophic lateral sclerosis

modeled by Zzzz\GGGGCC^30.UAS.EGFP

(Pun et al., 2022)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

NR1D2; nuclear receptor subfamily 1 group D member 2

5 of 14

602304

NR1D1; nuclear receptor subfamily 1 group D member 1

4 of 14

602408

RORA; RAR related orphan receptor A

4 of 14

600825

INTELLECTUAL DEVELOPMENTAL DISORDER WITH OR WITHOUT EPILEPSY OR CEREBELLAR ATAXIA; IDDECA

RORB; RAR related orphan receptor B

4 of 14

601972

EPILEPSY, IDIOPATHIC GENERALIZED, SUSCEPTIBILITY TO, 15; EIG15

RORC; RAR related orphan receptor C

4 of 14

602943

IMMUNODEFICIENCY 42; IMD42

immunodeficiency 42

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Eip78C - Bgb

two hybrid array

(Shokri et al., 2019)

Eip78C - Blimp-1

two hybrid array

(Shokri et al., 2019)

Eip78C - BtbVII

two hybrid array

(Shokri et al., 2019)

Eip78C - CG10209

two hybrid array, bimolecular fluorescence complementation, fluorescence microscopy

(Shokri et al., 2019)

Eip78C - CG10654

two hybrid array

(Shokri et al., 2019)

Eip78C - CG13204

two hybrid array

(Shokri et al., 2019)

Eip78C - CG14667

two hybrid array

(Shokri et al., 2019)

Eip78C - CG14711

two hybrid array

(Shokri et al., 2019)

Eip78C - CG15269

two hybrid array

(Shokri et al., 2019)

Eip78C - CG17287

two hybrid array

(Shokri et al., 2019)

Eip78C - CG31835

two hybrid array

(Shokri et al., 2019)

Eip78C - CG32264

two hybrid array

(Shokri et al., 2019)

Eip78C - CG32772

two hybrid array

(Shokri et al., 2019)

Eip78C - CG3726

two hybrid array

(Shokri et al., 2019)

Eip78C - CG3815

two hybrid array

(Shokri et al., 2019)

Eip78C - CG4424

two hybrid array

(Shokri et al., 2019)

Eip78C - CG6689

two hybrid array

(Shokri et al., 2019)

Eip78C - CG8159

two hybrid array

(Shokri et al., 2019)

Eip78C - Clamp

two hybrid array

(Shokri et al., 2019)

Eip78C - Dr

two hybrid array

(Shokri et al., 2019)

Eip78C - Hey

two hybrid array

(Shokri et al., 2019)

Eip78C - L

two hybrid array

(Shokri et al., 2019)

Eip78C - MBD-like

two hybrid array

(Shokri et al., 2019)

Eip78C - Mef2

two hybrid array

(Shokri et al., 2019)

Eip78C - Myb

two hybrid array

(Shokri et al., 2019)

Eip78C - Poxm

two hybrid array

(Shokri et al., 2019)

Eip78C - SoxN

two hybrid array

(Shokri et al., 2019)

Eip78C - Sry-β

two hybrid array

(Shokri et al., 2019)

Eip78C - TfAP-2

two hybrid array

(Shokri et al., 2019)

Eip78C - cbt

two hybrid array

(Shokri et al., 2019)

Eip78C - cyc

two hybrid array

(Shokri et al., 2019)

Eip78C - d4

two hybrid array

(Shokri et al., 2019)

Eip78C - da

two hybrid array

(Shokri et al., 2019)

Eip78C - drm

two hybrid array

(Shokri et al., 2019)

Eip78C - dsx

two hybrid array

(Shokri et al., 2019)

Eip78C - elB

two hybrid array

(Shokri et al., 2019)

Eip78C - gcm2

two hybrid array

(Shokri et al., 2019)

Eip78C - grh

two hybrid array

(Shokri et al., 2019)

Eip78C - gro

two hybrid array

(Shokri et al., 2019)

Eip78C - hkb

two hybrid array

(Shokri et al., 2019)

Eip78C - hry

two hybrid array

(Shokri et al., 2019)

Eip78C - ken

two hybrid array

(Shokri et al., 2019)

Eip78C - ktub

two hybrid array

(Shokri et al., 2019)

Eip78C - mbf1

two hybrid array

(Shokri et al., 2019)

Eip78C - ovo

two hybrid array

(Shokri et al., 2019)

Eip78C - ph-p

two hybrid array

(Shokri et al., 2019)

Eip78C - pho

two hybrid array

(Shokri et al., 2019)

Eip78C - trabl

two hybrid array

(Shokri et al., 2019)

Eip78C - unpg

two hybrid array

(Shokri et al., 2019)

Eip78C - zen

two hybrid array

(Shokri et al., 2019)

Eip78C - zfh1

two hybrid array

(Shokri et al., 2019)

Eip78C - zfh2

two hybrid array

(Shokri et al., 2019)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Eip78C

suppressible

Diap1

(Müller et al., 2005)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Zhu et al., 2005)

enhanceable

(Zhu et al., 2005)

enhanceable

(Zhu et al., 2005)

enhanceable

(Kim et al., 2010)

enhanceable

Eip78C

(Müller et al., 2005)

Rac1

enhanceable

Eip78C

(Zhu et al., 2005)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0004865

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene_with_start_codon_CUG

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-47

Cytogenetic map

78C2-78C3

Sequence location

3L:21,226,609..21,266,503 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

78C2-78C3

Limits computationally determined from genome sequence between P{EP}chb^EP3403 and P{PZ}ppl⁰⁰²¹⁷

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

78C4-78C6

(Martin-Blanco, 1993.4.20)

78D-78D

(determined by in situ hybridisation)

(Martin-Blanco and Kornberg, 1993)

Experimentally Determined Recombination Data

Location

3-47

(FlyBase, 2016)

3-43.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (32)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

91370

w¹¹¹⁸; Eip78C^Δ18

91371

w¹¹¹⁸; Eip78C^Δ37

49817

w¹¹¹⁸; P{GMR35B06-GAL4}attP2

54729

w¹¹¹⁸; P{GMR35B06-lexA}attP40

54770

w¹¹¹⁸; P{GMR35B09-lexA}attP40

44390

w^*; P{UAS-Eip78C.RNAi}attP16/CyO

91372

w¹¹¹⁸; PBac{UAS-Eip78C.A}VK00031

38637

w¹¹¹⁸; PBac{Eip78C-GFP.FLAG}VK00037

26718

y¹ v¹; P{TRiP.JF02258}attP2

FlyORF

F000656

M{UAS-Eip78C.ORF.3xHA.GW}ZH-86Fb

VDRC

v330304

P{VSH330304}attP40

v10396

w¹¹¹⁸ P{GD4135}v10396

More stocks available...

Genomic Clones (36)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (20)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

MIP25931

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0004865

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RE40623

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0004865

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Eip78C is required to establish the somatic germline stem cell niche in the ovary and it functions in the germline to promote the survival of developing follicles.

(Ables et al., 2015)

Gene expression is increased in response to the presence of two copies of Scer\GAL4^hs.PB.

(Liu and Lehmann, 2008)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

Normal Eip78C gene function is not required for viability or fertility, and its disruption has no visible phenotype. Deletion and overexpression of Eip78C demonstrates it function is required for the maximal induction of a subset of late puffs.

(Russell et al., 1996)

Eip78C expression is temporally shifted throughout the onset of metamorphosis by Eip74EF mutations.

(Fletcher and Thummel, 1995)

This ecdysone inducible gene encodes two nested transcription units. The larger encodes a member of the nuclear hormone receptor superfamily, the smaller encodes a truncated version that lacks the DNA binding domain.

(Stone and Thummel, 1993)

Eip78C gene structure and gene regulation have been determined.

(Stone and Thummel, 1992)

Relationship to Other Genes

Source for database merge of

Source for merge of: Eip78C anon-WO0172774.42 anon-WO0172774.44

(FlyBase, 1996-)

Additional comments

Source for merge of Eip78C anon-WO0172774.42 anon-WO0172774.44 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (20)

Reported As

Symbol Synonym

CG18023

(Meadows, 2017.1.11, Perkins et al., 2009, Perkins et al., 2009.3.31, Lee et al., 2003, Mount, 2001.8.14)

DR-78

(Martin-Blanco, 1993.4.20, Martin-Blanco and Kornberg, 1993)

E78A

(Praggastis et al., 2021, Sun and Song, 2006, Henrich and Brown, 1995, Horner et al., 1995, Thummel, 1993.8.27)

E78B

(Sinenko et al., 2010, Sun and Song, 2006, Sullivan and Thummel, 2003, Basso et al., 2002, Bialecki et al., 2002, Bryant et al., 1999, Richards et al., 1999, Fletcher et al., 1997, Lam et al., 1997, Fletcher and Thummel, 1995, Heimbeck et al., 1995, Henrich and Brown, 1995, Thummel, 1993.8.27)

E78D

(Stone and Thummel, 1992)

EIP78C

(Ritter and Beckstead, 2010)

Eip 78C

(Kaplow et al., 2007)

Eip78

(Shlyueva et al., 2014, Zinke et al., 1999)

Eip78D

Eip78c

(Ugrankar et al., 2018)

NR1E1

(King-Jones and Thummel, 2005, Nuclear Receptors Nomenclature Committee, 1999)

anon-WO0172774.42

anon-WO0172774.44

dE78

(Laudet and Adelmant, 1995)

eip78c

(Mazina et al., 2021)

Name Synonyms

Ecdysone-induced protein 78C

(Fichelson et al., 2012, Kaplow et al., 2007, Zeitouni et al., 2007, Cho et al., 2005)

ecdysone induced protein 78C

(Kim et al., 2010)

nuclear hormone receptor

(Russell, 1996.6.28, Russell, 1996.6.28)

Secondary FlyBase IDs

FBgn0046387
FBgn0046388

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 71 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40345

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P45447

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P45447

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

M9NDE9

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P45447

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40345/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004865

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004865

FlyMine - An integrated database for Drosophila genomics

FBgn0004865

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG18023

MARRVEL_MODEL - MARRVEL (model organism gene)

40345

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0004865

DroID - A comprehensive database of gene and protein interactions.

FBgn0004865

DRSC - Results frm RNAi screens

FBgn0004865

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004865

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0004865

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

Flygut - An atlas of the Drosophila adult midgut

FBgn0004865

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0004865

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC003935

References (162)

The following external sites may use different assemblies or annotations than FlyBase.

-0.44

Report Sections