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General Information
Symbol
Dmel\piwi
Species
D. melanogaster
Name
P-element induced wimpy testis
Annotation Symbol
CG6122
Feature Type
FlyBase ID
FBgn0004872
Gene Model Status
Stock Availability
Gene Summary
Acts via the piwi-interacting RNA (piRNA) metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (PubMed:26808625, PubMed:15817569, PubMed:17346786). Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements (PubMed:16882972). In ovarian somatic cells, mediates silencing of transposable elements at the transcriptional level in a mael-dependent manner (PubMed:23159368, PubMed:28472469). Involved in silencing of long terminal repeat (LTR) retrotransposons in male germline (PubMed:15817569). In testis, regulates spermatogenesis together with Tudor-SN (PubMed:26808625). In germ cells, mediates silencing at both transcriptional and post-transcriptional levels and is involved in the maintenance of populations of primary and secondary piRNAs. Piwi-mediated transcriptional silencing is accompanied by the formation of His3 'Lys-9' trimethylated (H3K9me3) euchromatin and heterochromatin (PubMed:23434410, PubMed:23392610). In ovary, associates predominantly with antisense piRNAs that contain uridine at their 5' end. Association with sense piRNAs is also observed but to a lesser extent. Mediates a somatic signaling mechanism required for the maintenance of germline stem cells to produce and maintain a daughter germline stem cell (PubMed:9851978, PubMed:10631171, PubMed:9199372, PubMed:16949822). It is not essential for the further differentiation of the committed daughter cell (PubMed:9851978). Acts cell autonomously to promote germline stem cell division (PubMed:9851978, PubMed:10631171). Its role in stem cell maintenance does not seem to require nuclear localization. Required maternally for the posterior localization of osk and vas and for pole cell formation during oogenesis and early embryogenesis (PubMed:16949822). Together with Hop and Hsp83, mediates canalization, also known as developmental robustness, likely via epigenetic silencing of existing genetic variants and suppression of transposon-induced new genetic variation (PubMed:21186352). Shows RNA cleavage activity, although is not required for any of its known functions (PubMed:9199372, PubMed:16882972, PubMed:23297219). In the ovaries, forms a complex with nxf2, Panx and Nxt1 which acts as effectors of cotranscriptional transposon silencing (PubMed:31368590, PubMed:31384064). (UniProt, Q9VKM1)
Contribute a Gene Snapshot for this gene.
Key Links
Genomic Location
Cytogenetic map
Sequence location
2L:10,982,205..10,987,420 [-]
Recombination map
2-44
RefSeq locus
NT_033779 REGION:10982205..10987420
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (32 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
inferred from physical interaction with UniProtKB:Q9VQ91
(assigned by UniProt )
inferred from physical interaction with UniProtKB:Q9U3V9
(assigned by UniProt )
inferred from physical interaction with UniProtKB:Q9VWE6
(assigned by UniProt )
inferred from physical interaction with UniProtKB:Q9W1F4
(assigned by UniProt )
inferred from physical interaction with UniProtKB:Q9V3H8,UniProtKB:Q9VV73,UniProtKB:Q9W2H9
(assigned by UniProt )
inferred from physical interaction with UniProtKB:A0A0B4K7J2
(assigned by UniProt )
inferred from physical interaction with FLYBASE:Fmr1; FB:FBgn0028734
inferred from physical interaction with FLYBASE:Dcr-1; FB:FBgn0039016
inferred from physical interaction with FLYBASE:vas; FB:FBgn0283442
enables RNA binding
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
Terms Based on Predictions or Assertions (0 terms)
Biological Process (17 terms)
Terms Based on Experimental Evidence (17 terms)
CV Term
Evidence
References
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from direct assay
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
involved_in oogenesis
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
involved_in spermatogenesis
inferred from mutant phenotype
(assigned by UniProt )
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (11 terms)
Terms Based on Experimental Evidence (11 terms)
CV Term
Evidence
References
located_in chromatin
inferred from direct assay
located_in chromocenter
inferred from direct assay
located_in cytoplasm
inferred from direct assay
(assigned by UniProt )
located_in cytosol
inferred from high throughput direct assay
located_in euchromatin
inferred from direct assay
inferred from direct assay
located_in heterochromatin
inferred from direct assay
located_in nucleoplasm
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
(assigned by UniProt )
located_in Yb body
inferred from physical interaction with FLYBASE:armi; FB:FBgn0041164
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the argonaute family. Piwi subfamily. (Q9VKM1)
Summaries
Protein Function (UniProtKB)
Acts via the piwi-interacting RNA (piRNA) metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (PubMed:26808625, PubMed:15817569, PubMed:17346786). Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements (PubMed:16882972). In ovarian somatic cells, mediates silencing of transposable elements at the transcriptional level in a mael-dependent manner (PubMed:23159368, PubMed:28472469). Involved in silencing of long terminal repeat (LTR) retrotransposons in male germline (PubMed:15817569). In testis, regulates spermatogenesis together with Tudor-SN (PubMed:26808625). In germ cells, mediates silencing at both transcriptional and post-transcriptional levels and is involved in the maintenance of populations of primary and secondary piRNAs. Piwi-mediated transcriptional silencing is accompanied by the formation of His3 'Lys-9' trimethylated (H3K9me3) euchromatin and heterochromatin (PubMed:23434410, PubMed:23392610). In ovary, associates predominantly with antisense piRNAs that contain uridine at their 5' end. Association with sense piRNAs is also observed but to a lesser extent. Mediates a somatic signaling mechanism required for the maintenance of germline stem cells to produce and maintain a daughter germline stem cell (PubMed:9851978, PubMed:10631171, PubMed:9199372, PubMed:16949822). It is not essential for the further differentiation of the committed daughter cell (PubMed:9851978). Acts cell autonomously to promote germline stem cell division (PubMed:9851978, PubMed:10631171). Its role in stem cell maintenance does not seem to require nuclear localization. Required maternally for the posterior localization of osk and vas and for pole cell formation during oogenesis and early embryogenesis (PubMed:16949822). Together with Hop and Hsp83, mediates canalization, also known as developmental robustness, likely via epigenetic silencing of existing genetic variants and suppression of transposon-induced new genetic variation (PubMed:21186352). Shows RNA cleavage activity, although is not required for any of its known functions (PubMed:9199372, PubMed:16882972, PubMed:23297219). In the ovaries, forms a complex with nxf2, Panx and Nxt1 which acts as effectors of cotranscriptional transposon silencing (PubMed:31368590, PubMed:31384064).
(UniProt, Q9VKM1)
Summary (Interactive Fly)

a novel protein involved in stem cell renewal and asymmetric cell division - piRNA-guided slicing of transposon transcripts enforces their transcriptional silencing via specifying the nuclear piRNA repertoire - maintains germline stem cells and oogenesis in Drosophila through negative regulation of Polycomb group proteins Piwi modulates chromatin accessibility by regulating multiple factors including Histone H1 to repress transposons

Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\piwi for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0080166
3073
843
FBtr0340227
2969
843
Additional Transcript Data and Comments
Reported size (kB)

3.1 (northern blot)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0079755
97.2
843
10.00
FBpp0309202
97.2
843
10.00
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

843 aa isoforms: piwi-PA, piwi-PB
Additional Polypeptide Data and Comments
Reported size (kDa)

843 (aa); 97.2 (kD predicted)

Comments
External Data
Subunit Structure (UniProtKB)

In the ovaries, part of a complex composed of at least Panx, nxf2, piwi and Nxt1 (PubMed:26472911, PubMed:26494711, PubMed:31368590, PubMed:31384064). The complex is knowns as Panx-induced co-transcriptional silencing (PICTS) complex, Panx-nxf2-dependent TAP/p15 silencing (Pandas complex), SFiNX (silencing factor interacting nuclear export variant) or piwi-Panx-nxf2-p15 (PPNP) complex (PubMed:26472911, PubMed:26494711, PubMed:31368590, PubMed:31384064). Interacts with vas; this interaction is RNA-independent (PubMed:16949822). Interacts with Dcr-1 and Fmr1; these interactions occur in polar granules (PubMed:16949822). Interacts (via N-terminal region) with CBX5 (via chromoshadow domain) (PubMed:17875665). Forms a complex with Hsp83 and Hop; probably Hop mediates the interaction between piwi and Hsp83 (PubMed:21186352). Forms a complex with Yb body components armi and fs(1)Yb; this interaction is required for proper piRNA loading and nuclear localization of piwi (PubMed:20966047). Interaction of Piwi and fs(1)Yb is likely to occur via armi (PubMed:20966047). Interacts (via the N-terminal region when unmethylated or symmetrically methylated at Arg-10) with papi (via Tudor domain) (PubMed:21447556, PubMed:29531043). Interacts with vret (PubMed:21831924). Interacts with Panx (PubMed:26472911, PubMed:26494711). Interacts with arx (PubMed:23913921, PubMed:23913922). Interacts with Tudor-SN (PubMed:26808625). Interacts with Nup358 (via N-terminus) (PubMed:29735528). Associates with the nuclear pore complex via interaction with Elys (PubMed:28472469). Interacts with thoc5; the interaction might be partly RNA-mediated (PubMed:28472469). Interacts with xmas-2 (PubMed:28472469).

(UniProt, Q9VKM1)
Post Translational Modification

Symmetrically dimethylated, most likely by csul (PubMed:19377467, PubMed:29531043). Methylation at Arg-10 enhances binding to papi whereas methylation at Arg-7, Arg-9 or Arg-11 reduces binding affinity to papi (PubMed:29531043).

Phosphorylated on serine and tyrosine residues in an Hsp83-dependent manner.

(UniProt, Q9VKM1)
Crossreferences
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\piwi using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
pole plasm

Comment: maternally deposited

organism

Comment: maternally deposited

RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

piwi transcript is detected in germline and soma cells during oogenesis. In the germarium, expression is detected in the terminal filament cells and the epithelial sheath cells. In germ cells, expression is detected as early as germarium region 2, continues during stages S1-S6 of oogenesis, is much diminished but still detectable between S7-S9, and is again at high levels at stage S10. In early embryos, expression is uniform.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in chromatin
inferred from direct assay
located_in chromocenter
inferred from direct assay
located_in cytoplasm
inferred from direct assay
(assigned by UniProt )
located_in cytosol
inferred from high throughput direct assay
located_in euchromatin
inferred from direct assay
inferred from direct assay
located_in heterochromatin
inferred from direct assay
located_in nucleoplasm
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
(assigned by UniProt )
located_in Yb body
inferred from physical interaction with FLYBASE:armi; FB:FBgn0041164
Expression Deduced from Reporters
Stage
Tissue/Position (including subcellular localization)
Reference
adult brain

Comment: punctate expression throughout adult brain

High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\piwi in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
EMBL-EBI Single Cell Expression Atlas
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 9-10
  • Stages(s) 13-16
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 21 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 67 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of piwi
Transgenic constructs containing regulatory region of piwi
Aberrations (Deficiencies and Duplications) ( 3 )
Inferred from experimentation ( 3 )
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
9 of 15
Yes
Yes
8 of 15
No
No
6 of 15
No
No
6 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
7 of 15
Yes
No
6 of 15
No
No
6 of 15
No
Yes
Rattus norvegicus (Norway rat) (3)
8 of 13
Yes
Yes
6 of 13
No
No
5 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (4)
7 of 12
Yes
Yes
6 of 12
No
Yes
5 of 12
No
No
2 of 12
No
Yes
Danio rerio (Zebrafish) (2)
8 of 15
Yes
Yes
6 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (9)
10 of 15
Yes
No
7 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (7)
2 of 9
Yes
Yes
2 of 9
Yes
Yes
1 of 9
No
Yes
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (1)
1 of 12
Yes
No
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091902E1 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502F7 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Mayetiola destructor
Hessian fly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W027S )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X01B5 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G020J )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (4)
6 of 10
5 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser

Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
RNA-protein
Physical Interaction
Assay
References
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Subunit Structure (UniProtKB)
In the ovaries, part of a complex composed of at least Panx, nxf2, piwi and Nxt1 (PubMed:26472911, PubMed:26494711, PubMed:31368590, PubMed:31384064). The complex is knowns as Panx-induced co-transcriptional silencing (PICTS) complex, Panx-nxf2-dependent TAP/p15 silencing (Pandas complex), SFiNX (silencing factor interacting nuclear export variant) or piwi-Panx-nxf2-p15 (PPNP) complex (PubMed:26472911, PubMed:26494711, PubMed:31368590, PubMed:31384064). Interacts with vas; this interaction is RNA-independent (PubMed:16949822). Interacts with Dcr-1 and Fmr1; these interactions occur in polar granules (PubMed:16949822). Interacts (via N-terminal region) with CBX5 (via chromoshadow domain) (PubMed:17875665). Forms a complex with Hsp83 and Hop; probably Hop mediates the interaction between piwi and Hsp83 (PubMed:21186352). Forms a complex with Yb body components armi and fs(1)Yb; this interaction is required for proper piRNA loading and nuclear localization of piwi (PubMed:20966047). Interaction of Piwi and fs(1)Yb is likely to occur via armi (PubMed:20966047). Interacts (via the N-terminal region when unmethylated or symmetrically methylated at Arg-10) with papi (via Tudor domain) (PubMed:21447556, PubMed:29531043). Interacts with vret (PubMed:21831924). Interacts with Panx (PubMed:26472911, PubMed:26494711). Interacts with arx (PubMed:23913921, PubMed:23913922). Interacts with Tudor-SN (PubMed:26808625). Interacts with Nup358 (via N-terminus) (PubMed:29735528). Associates with the nuclear pore complex via interaction with Elys (PubMed:28472469). Interacts with thoc5; the interaction might be partly RNA-mediated (PubMed:28472469). Interacts with xmas-2 (PubMed:28472469).
(UniProt, Q9VKM1 )
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
External Data
Linkouts
KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2L
Recombination map
2-44
Cytogenetic map
Sequence location
2L:10,982,205..10,987,420 [-]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
32C1-32C1
Limits computationally determined from genome sequence between P{lacW}l(2)k05812k05812&P{lacW}l(2)k09104k09104 and P{EP}ppoEP2478&P{lacW}l(2)k15817k15817
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
32B-32C
(determined by in situ hybridisation)
32C-32C
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (17)
Genomic Clones (21)
cDNA Clones (82)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
RNAi and Array Information
Linkouts
DRSC - Results frm RNAi screens
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Laboratory Generated Antibodies
Commercially Available Antibodies
 
Other Information
Relationship to Other Genes
Source for database identify of

Source for identity of: piwi CG6122

Source for database merge of
Additional comments
Other Comments

piwi-RISC (RNA-induced silencing complex) mediates silencing of transposable elements at the transcriptional level, and this is accompanied by local heterochromatin formation.

piwi is required for maintenance of long-distance chromosomal interactions between endogenous PcG target loci.

piwi protein is localised to the nucleus and is specifically associated with rasiRNAs (repeat-associated small RNAs) derived from various repetitive elements in the genome such as retrotransposons and heterochromatic regions in vivo and shows shows target RNA cleavage ("Slicer") activity in the presence of a single-stranded guide siRNA in vitro.

Expression is enriched in embryonic gonads.

The piwi product binds directly to repeat-associated small interfering RNAs (rasiRNAs).

piwi is required for the accumulation of repeat-associated small interfering RNAs (rasiRNAs).

piwi is required for the silencing of retrotransposons in the male germline.

bam is epistatic to somatic niche piwi function in the ovary, but germline piwi is epistatic to bam function in the ovary.

Mutations in piwi or aub result in partial loss of the methyl-K9 form of His3, most evident at minor sites within the euchromatic arms.

piwi, aub and spn-E are suppressors of repeat-induced silencing, as seen at heterochromatic tandem arrays of insertions of the P{lacW} transposon.

piwi has a cell autonomous function in promoting stem cell division in the germline.

piwi mediates a somatic signalling mechanism required for the asymmetric division of germ-line stem cells to produce and maintain a daughter germ-line stem cell but is not essential for the further differentiation of the committed daughter cell.

Mutant analysis demonstrates the piwi function is required both to maintain germline stem cells and subsequently for the division and differentiation of the stem cell progeny in both sexes. Mutations abolish the proliferation ability of both female and male germline stem cells.

piwi gene product affects the asymmetric division of the male and female germline stem cell (GSC).

Origin and Etymology
Discoverer
Etymology
Identification
External Crossreferences and Linkouts ( 46 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
Other crossreferences
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
EMBL-EBI Single Cell Expression Atlas
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
KEGG Genes - Molecular building blocks of life in the genomic space.
MARRVEL_MODEL
modMine - A data warehouse for the modENCODE project
Linkouts
ApoDroso - Functional genomic database for photoreceptor development, survival and function
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
DRSC - Results frm RNAi screens
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
FlyMine - An integrated database for Drosophila genomics
Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
MIST (protein-protein) - An integrated Molecular Interaction Database
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
Piwi
(Eastwood et al., 2021, Schneider and Imler, 2021, Adashev et al., 2020, Gonzalez et al., 2020, Kelleher et al., 2020, Lepesant et al., 2020, Mugat et al., 2020, Ninova et al., 2020, Sato and Siomi, 2020, Tindell et al., 2020, Tsuboyama et al., 2020, Yamaguchi et al., 2020, Zhang and Cai, 2020, Batki et al., 2019, Fabry et al., 2019, Ge et al., 2019, Hirakata et al., 2019, Ishizu et al., 2019, Munafò et al., 2019, Murano et al., 2019, Russo et al., 2019, Schüpbach, 2019, Specchia et al., 2019, Zhao et al., 2019, Jankovics et al., 2018, Parikh et al., 2018, Rojas-Ríos and Simonelig, 2018, Sato and Siomi, 2018, Yamashiro and Siomi, 2018, Yashiro et al., 2018, Zhang et al., 2018, Dehghani and Lasko, 2017, Fefelova et al., 2017, Huang et al., 2017, Hyun, 2017, Ilyin et al., 2017, Karam et al., 2017, Luo and Lu, 2017, Pandey et al., 2017, Rogers et al., 2017, Ryazansky et al., 2017, Vrettos et al., 2017, Yang and Xi, 2017, Haase, 2016, Hayashi et al., 2016, Hirakata and Siomi, 2016, Jiang et al., 2016, Kelleher, 2016, Kotov et al., 2016, Ku et al., 2016, Lewis et al., 2016, Peng et al., 2016, Wang et al., 2016, Gonzalez et al., 2015, Han et al., 2015, Lin et al., 2015, Malone et al., 2015, Meller et al., 2015, Molla-Herman et al., 2015, Sato and Siomi, 2015, Sato et al., 2015, Hamada-Kawaguchi et al., 2014, Matzat and Lei, 2014, McElroy et al., 2014, Murota et al., 2014, Patil et al., 2014, Bausek, 2013, Czech et al., 2013, Darricarrère et al., 2013, Gomes et al., 2013, Guzzardo et al., 2013, Lasko, 2013, Le Thomas et al., 2013, Mani and Juliano, 2013, Ohtani et al., 2013, Olovnikov and Kalmykova, 2013, Peng and Lin, 2013, Rozhkov et al., 2013, Saito, 2013, Seervai and Wessel, 2013, Sentmanat et al., 2013, Vagin et al., 2013, Anand and Kai, 2012, Bozzetti et al., 2012, Kelleher et al., 2012, Kibanov et al., 2012, Kumar and Chen, 2012, McCue and Slotkin, 2012, Nishimasu et al., 2012, Olivieri et al., 2012, Preall et al., 2012, Stefanov et al., 2012, Vazquez-Pianzola and Suter, 2012, Eliazer and Buszczak, 2011, Ishizu et al., 2011, Khurana et al., 2011, Pane et al., 2011, Pek and Kai, 2011, Sato et al., 2011, Tiwari et al., 2011, Zhang et al., 2011, Kirino et al., 2010, Rigoutsos, 2010, Klattenhoff et al., 2009, Li et al., 2009, Miyoshi et al., 2009, Nishida et al., 2009, Czech et al., 2008, Kawamura et al., 2008, Lei et al., 2008, Shpiz et al., 2008, Antoniewski et al., 2007, Gunawardane et al., 2007, Gunawardane et al., 2007, Klenov et al., 2007, Lei, 2007, Lin, 2007, Nishida et al., 2007, Peng and Karpen, 2007, Prasanth and Spector, 2007, Saito et al., 2007, Yin and Lin, 2007, Zamore, 2007, Carthew, 2006, Dorner et al., 2006, Nystul and Spradling, 2006, Zamore, 2006, Gim et al., 2001)
piwi
(Proshkina et al., 2021, Yang, 2021, Angelova et al., 2020, Duan et al., 2020, Ellison et al., 2020, Fort-Aznar et al., 2020, Hinnant et al., 2020, Kordyukova et al., 2020, Onishi et al., 2020, Parker et al., 2020, Chang et al., 2019, Kina et al., 2019, Kneuss et al., 2019, Kolliopoulou et al., 2019, Kotov et al., 2019, Molnar et al., 2019, Park et al., 2019, Schaub et al., 2019, Stein et al., 2019, Trcek and Lehmann, 2019, Wakisaka et al., 2019, Whittle and Extavour, 2019, Yang et al., 2019, Gene Disruption Project members, 2018-, Hao et al., 2018, Hughes et al., 2018, Kang et al., 2018, Levis, 2018.8.30, Ma et al., 2018, Mehrotra and Deshpande, 2018, Tasnim and Kelleher, 2018, Théron et al., 2018, Tian and Wang, 2018, van den Beek et al., 2018, Akkouche et al., 2017, Marie et al., 2017, Rashpa et al., 2017, Sousa-Victor et al., 2017, Specchia et al., 2017, Teixeira et al., 2017, Basu et al., 2016, Blumenstiel et al., 2016, Fagegaltier et al., 2016, Na et al., 2016, Reid and O'Brochta, 2016, Wang et al., 2016, Flores et al., 2015, Ishizu et al., 2015, Mikhaleva et al., 2015, Mugat et al., 2015, Onorati et al., 2015, Sato et al., 2015, Senti et al., 2015, Sienski et al., 2015, Yu et al., 2015, Zhang et al., 2015, Atikukke et al., 2014, Basquin et al., 2014, Blanchard et al., 2014, Dufourt et al., 2014, Hamada-Kawaguchi et al., 2014, Hayashi et al., 2014, Le Thomas et al., 2014, Lim et al., 2014, Ma et al., 2014, Mani et al., 2014, Minakhina et al., 2014, Pargett et al., 2014, Ren et al., 2014, Ross et al., 2014, Wang et al., 2014, Bausek, 2013, Czech et al., 2013, Darricarrère et al., 2013, Dennis et al., 2013, Dönertas et al., 2013, Gell and Reenan, 2013, Handler et al., 2013, Huang et al., 2013, Jin et al., 2013, Muerdter et al., 2013, Ohtani et al., 2013, Bozzetti et al., 2012, Japanese National Institute of Genetics, 2012.5.21, McEachern, 2012, Meier et al., 2012, Menon and Meller, 2012, Siddiqui et al., 2012, Sienski et al., 2012, Simmons et al., 2012, Zhang et al., 2012, Castillo et al., 2011, Ding and Lu, 2011, Eliazer and Buszczak, 2011, Gangaraju et al., 2011, Handler et al., 2011, Klenov et al., 2011, Klenov et al., 2011, Liu et al., 2011, Miles et al., 2011, Ni et al., 2011, Parrott et al., 2011, Pek and Kai, 2011, Pek and Kai, 2011, Poulton et al., 2011, Qi et al., 2011, Shpiz et al., 2011, Tchurikov and Kretova, 2011, Wang and Elgin, 2011, Zamparini et al., 2011, Fernández-Ayala et al., 2010, Haase et al., 2010, Janic et al., 2010, Lu and Clark, 2010, Moshkovich and Lei, 2010, Nagao et al., 2010, Olivieri et al., 2010, Rouget et al., 2010, Saito et al., 2010, Simmons et al., 2010, Smulders-Srinivasan et al., 2010, Stefanov et al., 2010, Thomsen et al., 2010, Belinco et al., 2009, Chau et al., 2009, Habayeb et al., 2009, Jepson and Reenan, 2009, Kavi and Birchler, 2009, Kirino et al., 2009, Lau et al., 2009, Li and Lu, 2009, Malone et al., 2009, Robine et al., 2009, Saito et al., 2009, Shpiz et al., 2009, Szakmary et al., 2009, Venken et al., 2009, Berdnik et al., 2008, Blumenstiel et al., 2008, Chambeyron et al., 2008, Chau et al., 2008, Chotkowski et al., 2008, Cross and Simmons, 2008, Desset et al., 2008, Kalmykova et al., 2008, Kavi and Birchler, 2008, McGraw et al., 2008, Ronsseray et al., 2008, Bhadra et al., 2007, Brennecke et al., 2007, Brower-Toland et al., 2007, Georlette et al., 2007, Glaser et al., 2007, Josse et al., 2007, Kavi and Birchler, 2007, Kruger, 2007, Pelisson et al., 2007, Ronsseray et al., 2007, Shpiz et al., 2007, Simmons et al., 2007, Sofola et al., 2007, Wu and Silverman, 2007, Xing et al., 2007, Yang et al., 2007, Zaratiegui, 2007, Christensen and Cook, 2006.8.30, Haynes et al., 2006, Kavi et al., 2006, Lei and Corces, 2006, Lei and Corces, 2006, Megosh et al., 2006, Musters et al., 2006, Payne and Braun, 2006, Saito et al., 2006, Shigenobu et al., 2006, Vagin et al., 2006, Vagin et al., 2006, Kalmykova et al., 2005, Xie et al., 2005, Gonzalez-Reyes, 2003)
Secondary FlyBase IDs
    Datasets (3)
    Study focus (3)
    Experimental Role
    Project
    Project Type
    Title
    • bait_protein
    Genome-wide localization of chromosomal proteins in cell lines by ChIP-chip and ChIP-Seq.
    • allele_used
    Genome-wide localization of chromosomal proteins in fly tissues by ChIP-chip and ChIP-Seq.
    • allele_used
    Genome-wide localization of histones and their modifications in fly tissues by ChIP-chip and ChIP-Seq.
    References (494)