TSC2, dTsc2, l(3)109
Rap1 GAP domain protein - tuberous sclerosis-2 homolog - regulates growth rate - modifies the insulin pathway - maintains Drosophila germline stem cells by preventing differentiation
Please see the JBrowse view of Dmel\gig for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.45
6.2 (northern blot)
None of the polypeptides share 100% sequence identity.
1847 (aa)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\gig using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
JBrowse - Visual display of RNA-Seq signals
View Dmel\gig in JBrowse3-46
3-43.5
This map location corrects that reported in FBrf0076453.
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
dsRNA made from templates generated with primers directed against this gene results in an increase in mean cell diameter.
dsRNA made from templates generated with primers directed against CG32343, CG7845, CG10805, CG12301, CG5114, CG5033, CG10648, CG7006, CG3071, CG1542, bys, Ski6, RpI135, l(1)G0020, ppan, Nnp-1, NHP2, or pit in combination with dsRNA against gig results in suppression of the final average cell size, with it being closer to the control average cell size, rather than the average cell size resulting from knockdown of the candidate gene alone.
S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.
dsRNA made from templates generated with primers directed against this gene decreases the level of Akt1 phosphorylation, especially in response to insulin stimulation, in S2 cells.
One of 42 Drosophila genes identified as being most likely to reveal molecular and cellular mechanisms of nervous system development or plasticity relevant to human Mental Retardation disorders.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Area matching Drosophila EST AI456286. Probable intron in gene represented by EST AI456286.
gig is a negative regulator of insulin signalling.
In contrast to the findings reported in FBrf0107789, it has become apparent that while gig mutant cells are enlarged, there is no endoreduplication of their DNA.
Mutations in gig result in enhanced growth and cell size with no change in ploidy. Overall, mutant cells spend less time in G1.
gig blocks rereplication of DNA or promotes mitosis during imaginal disc development.
Study of synaptic frequency in the enlarged neurons of mutants reveals a causal relationship between cell size and synaptic number: the frequency of synaptic profiles increases as the size of terminal perimeters is increased.
'gigas' means 'giant' in Latin.
