conserved Cys and His domains - ubiquitin-specific protease - plays multiple roles in eye and oocyte development - Fat facets and Liquid facets promote Delta endocytosis and Delta signaling
Gene model reviewed during 5.49
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.53
8.9, 8.5 (compiled cDNA)
2747, 2711 (aa); 300 (kD predicted)
Interacts with imd.
Ubiquitinated. Ubiquitination is enhanced by the expression of imd.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\faf using the Feature Mapper tool.
faf transcripts are detected in the germarium and in the egg chambers at subsequent stages of oogenesis. faf transcripts are observed in nurse cells and unlike faf protein, do not localize to the posterior of the oocyte at stage S10. The probe used does not distinguish between the two faf transcripts.
faf protein distribution was deduced from the localization of a fafFBtr0091350:pb-XRlacZ fusion protein. faf protein is detected in eye imaginal discs ahead of and behind the morphogenetic furrow but not in the furrow. It is also observed in other imaginal discs, fat body, gut, larval ovary and testis, and adult male sex organs. faf protein is detected in ovaries in the germarium and at all subsequent stages of oogenesis. Staining is restricted to the nurse cells until stage S10 when it becomes apparent at the oocyte posterior pole. Posterior localization of faf is dependent on osk.
faf protein distribution was deduced from the localization of a fafFBtr0091350:pb-XRlacZ fusion protein. faf protein is detected in eye imaginal discs ahead of the morphogenetic furrow and at a lower level behind the furrow but not in the furrow. It is also observed in other imaginal discs, fat body, gut, larval ovary and testis, and adult male sex organs. faf protein is detected in ovaries in the germarium and at all subsequent stages of oogenesis. Staining is restricted to the nurse cells until stage S10 when it becomes apparent at the oocyte posterior pole. Posterior localization of faf is dependent on osk.
GBrowse - Visual display of RNA-Seq signalsView Dmel\faf in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
faf de-ubiquitination has a role in controlling the level of activity of critical regulatory molecules at the synapse.
Analysis of genetic interactions among faf and R and Ras85D reveals that in addition to its critical role anterior to the morphogenetic furrow, faf has a function in undifferentiated cells alter in eye development that involves, probably indirectly, Ras85D and R. These results suggest that cells outside the facet influence cell fates within the facet.
faf is essential only early in eye development and only outside of the 7-cell precluster and cone cell precursors. The critical role of faf in eye development is not in the regulation of argos, aop or Gap1 activities, nor in the regulation of any other protein that functions within cells of the developing facets. Undifferentiated cells outside the facet precluster direct ommatidial assembly by sending to the mystery cells and other cells an inhibitory signal, regulated by faf protein.
The faf protein acts as a regulatory Ubiquitin-specific protease that prevents degradation of its substrate by the proteasome. The faf Ubiquitin-specific protease is cell type- and substrate-specific and regulates cell fate decisions.
faf is required for cell interactions that prevent particular cells in the developing eye from becoming photoreceptors. Mosaic analysis indicates that faf is required in cells close to, but outside the normal developing photoreceptors and also outside the ectopic photoreceptors in mutant facets. The faf product is also required during oogenesis (embryos from faf mutant mothers never cellularize, except for the pole cells), but is not required for nos localization or function. The faf gene has been cloned, and all regions essential for its function identified by transformation rescue of the mutant phenotypes. faf-Ecol\lacZ chimeric proteins become posteriorly localized in oocytes, and the first 392 amino acids of faf protein is sufficient for this localization.