Gene model reviewed during 5.41
Gene model reviewed during 5.45
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Con using the Feature Mapper tool.
Expression pattern inferred from unspecified enhancer trap line.
Con transcripts are first detected at stage 10 in a subset of the cells that will give rise to the visceral mesoderm. There is expression in the presumptive foregut and hindgut mesoderm and in a segmentally repeated subset of the presumptive midgut visceral mesoderm. At late stage 11, transcripts appear in the CNS and in the somatic mesoderm. The initial expression in the somatic mesoderm is strong in the thoracic segments and relatively weak in the abdominal segments. By stage 14, the intensity differences in staining of the somatic mesoderm have subsided by there are differences in the arrangement of staining cells in the thoracic and abdominal segments. In the CNS, the initially uniform staining is succeeded by a more modulated pattern. Differences appear by stage 14 and by stage 16, there is strong staining in the anterior CNS, including the brain lobes and the thoracic neuromeres but little labeling in the abdominal neuromeres. Labelling is also seen in gnathal sense organs.
Con is expressed on the surface of eight muscles, the motoneurons that innervate them, and several glial cells along the pathways leading to them. During synapse formation, Con protein localizes to the synaptic sites and afterwards it largely disappears. Expression is also observed in a subset of neurons and glia in the CNS, in the dorsal cluster of the PNS and in the visceral mesoderm.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Con in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Ectopic expression of Con in all muscles causes SNa motorneurons to often inappropriately innervate a neighbouring non-target muscle that ectopically expresses Con. The ectopic synapse formation is dependent on the endogenous Con expression on the SNa motoneurons. Results show that Con can function as an attractive and homophilic target recognition molecule in vivo. Con has a specific function during SNb pathfinding.
The pattern of expression of Con protein during embryogenesis has been analysed.
In pros mutants, where innervation of muscles fails, connectin is expressed on muscle surface begins in same developmental pattern as in wild type.
A chromatin immunopurification approach isolated the Connectin gene as a binding site for Ubx in vivo. The product is predicted to be a cell surface molecule containing leucine rich repeats and can mediate cell-cell adhesion.
Con is a novel cell adhesion molecule whose expression suggests a role in target recognition.
The genes encoding the 35 and 48 transcripts, Con and T48 respectively, are good candidates for the target genes directly regulated by the homeotic gene Ubx.
Cloned as a potential Ubx target gene.