Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\HIS-C using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\HIS-C in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
The Histone gene family exists as a discontinuous array of approximately 100 repeats, 8-12 blocks of histone repeats of approximately 10 copies each. Most arrays have multiple repeats with at least one restriction site not present in the published sequence. The repeats with these nonconsensus sites are adjacent to each other, or much less often are separated by a repeat. Therefore movement of variants in the gene family is predominantly to nearest neighbours. The sequence similarity should be a monotonically decreasing function of distance along the histone array.
Topoisomerase II cleavage sites in the HIS-C repeat have been studied in vitro and in vivo.
HIS-C refers collectively to five structural genes (His1, His2A, His2B, His3, and His4) for the five different histones (H1, H2A, H2B, H3 and H4). H2A, H2B, H3 and H4 participate in equimolar quantities in the formation of histone octomers, which form the protein core of nucleosomes. Heterozygosity for full or partial deficiency of the histone genes suppresses variegation (BSV, SbV, wm4); duplications without effect on level of variegation. Transcription not repressed by heat shock.
The expression of HIS-C genes during oogenesis has been studied, and compared to periods of DNA synthesis and actin expression during this developmental stage.
4.8kb and 5.0kb repeats containing the histone genes His1, His2A, His2B, His3 and His4 are present in all of the more than 20 D.melanogaster strains studied. The strains differ in the relative amounts of the two repeat types, with the 5.0kb repeat always present in equal or greater amounts than the 4.8kb repeat. The strains also differ in a number of far less abundant fragments containing histone gene sequences.